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Article ; Online: The Characterization of

Steyn, Angela / Keep, Sarah / Bickerton, Erica / Fife, Mark

2020 Volume 11, Issue 8

Abstract: The coronaviruses are a large family of enveloped RNA viruses that commonly cause gastrointestinal or respiratory illnesses in the infected host. Avian coronavirus infectious bronchitis virus (IBV) is a highly contagious respiratory pathogen of chickens ... ...

Abstract	The coronaviruses are a large family of enveloped RNA viruses that commonly cause gastrointestinal or respiratory illnesses in the infected host. Avian coronavirus infectious bronchitis virus (IBV) is a highly contagious respiratory pathogen of chickens that can affect the kidneys and reproductive systems resulting in bird mortality and decreased reproductivity. The interferon-inducible transmembrane (IFITM) proteins are activated in response to viral infections and represent a class of cellular restriction factors that restrict the replication of many viral pathogens. Here, we characterize the relative mRNA expression of the chicken
MeSH term(s)	Animals ; Chickens/genetics ; Chickens/virology ; Coronavirus Infections/genetics ; Coronavirus Infections/veterinary ; Gene Expression Regulation, Viral ; Host-Pathogen Interactions/genetics ; Host-Pathogen Interactions/physiology ; Infectious bronchitis virus/pathogenicity ; Infectious bronchitis virus/physiology ; Membrane Proteins/genetics ; Organ Culture Techniques ; Poultry Diseases/etiology ; Poultry Diseases/genetics ; Poultry Diseases/virology ; Viral Load ; Viral Tropism
Chemical Substances	Membrane Proteins
Keywords	covid19
Language	English
Publishing date	2020-08-10
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes11080918
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The Characterization of chIFITMs in Avian Coronavirus Infection In Vivo, Ex Vivo and In Vitro

Steyn, Angela / Keep, Sarah / Bickerton, Erica / Fife, Mark

Genes. 2020 Aug. 10, v. 11, no. 8

2020

Abstract	The coronaviruses are a large family of enveloped RNA viruses that commonly cause gastrointestinal or respiratory illnesses in the infected host. Avian coronavirus infectious bronchitis virus (IBV) is a highly contagious respiratory pathogen of chickens that can affect the kidneys and reproductive systems resulting in bird mortality and decreased reproductivity. The interferon-inducible transmembrane (IFITM) proteins are activated in response to viral infections and represent a class of cellular restriction factors that restrict the replication of many viral pathogens. Here, we characterize the relative mRNA expression of the chicken IFITM genes in response to IBV infection, in vivo, ex vivo and in vitro using the pathogenic M41-CK strain, the nephropathogenic QX strain and the nonpathogenic Beaudette strain. In vivo we demonstrate a significant upregulation of chIFITM1, 2, 3 and 5 in M41-CK- and QX-infected trachea two days post-infection. In vitro infection with Beaudette, M41-CK and QX results in a significant upregulation of chIFITM1, 2 and 3 at 24 h post-infection. We confirmed a differential innate response following infection with distinct IBV strains and believe that our data provide new insights into the possible role of chIFITMs in early IBV infection.
Keywords	Coronavirus infections ; Infectious bronchitis virus ; chickens ; gastrointestinal system ; gene expression ; genes ; kidneys ; messenger RNA ; mortality ; proteins ; respiratory tract diseases
Language	English
Dates of publication	2020-0810
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2527218-4
ISSN	2073-4425
ISSN	2073-4425
DOI	10.3390/genes11080918
Database	NAL-Catalogue (AGRICOLA)

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Article: Zn Fertilizer and Mycorrhizal Inoculation Effect on Bread Wheat Cultivar Grown under Water Deficit.

Abdi, Neila / Van Biljon, Angeline / Steyn, Chrisna / Labuschagne, Maryke

Life (Basel, Switzerland)

2023 Volume 13, Issue 5

Abstract: During drought stress, many enzymes are inactivated in plants due to Zn deficiency. Zn application and arbuscular mycorrhiza fungi (AMF)-wheat symbiosis reportedly improve the tolerance of plants to drought stress. This study was done to investigate the ... ...

Abstract	During drought stress, many enzymes are inactivated in plants due to Zn deficiency. Zn application and arbuscular mycorrhiza fungi (AMF)-wheat symbiosis reportedly improve the tolerance of plants to drought stress. This study was done to investigate the effect of Zn and AMF on plant growth, yield attributes, relative water content (RWC), harvest index (HI), photosynthetic activity, solute accumulation, glycine betaine (GB) accumulation, antioxidant activities [(catalase (CAT) and superoxide dismutase (SOD)], and ionic attributes in a bread wheat cultivar (SST806) under drought-stress in plants grown under greenhouse conditions. Zn application and AMF inoculation, separately and combined, enhanced all plant growth parameters and yield. Root dry weight (RDW) was increased by 25, 30, and 46% for these three treatments, respectively, under drought conditions compared to the control treatment. Overall, Zn application, AMF inoculation, and their combination increased protein content, RWC, and harvest index (HI) under drought stress. However, AMF inoculation improved proline content more than Zn application under the same conditions. Regarding GB accumulation, AMF, Zn, and the combination of Zn and AMF increased GB under drought compared to well-watered conditions by 31.71, 10.36, and 70.70%, respectively. For the antioxidant defense, AMF inoculation and Zn application improved SOD and CAT activity by 58 and 56%, respectively. This study showed that Zn and/or AMF increased antioxidant levels and ionic attributes under abiotic stress.
Language	English
Publishing date	2023-04-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life13051078
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Article: The Characterization of chIFITMs in Avian Coronavirus Infection In Vivo, Ex Vivo and In Vitro

Steyn, Angela / Keep, Sarah / Bickerton, Erica / Fife, Mark

Abstract	The coronaviruses are a large family of enveloped RNA viruses that commonly cause gastrointestinal or respiratory illnesses in the infected host. Avian coronavirus infectious bronchitis virus (IBV) is a highly contagious respiratory pathogen of chickens that can affect the kidneys and reproductive systems resulting in bird mortality and decreased reproductivity. The interferon-inducible transmembrane (IFITM) proteins are activated in response to viral infections and represent a class of cellular restriction factors that restrict the replication of many viral pathogens. Here, we characterize the relative mRNA expression of the chicken IFITM genes in response to IBV infection, in vivo, ex vivo and in vitro using the pathogenic M41-CK strain, the nephropathogenic QX strain and the nonpathogenic Beaudette strain. In vivo we demonstrate a significant upregulation of chIFITM1, 2, 3 and 5 in M41-CK- and QX-infected trachea two days post-infection. In vitro infection with Beaudette, M41-CK and QX results in a significant upregulation of chIFITM1, 2 and 3 at 24 h post-infection. We confirmed a differential innate response following infection with distinct IBV strains and believe that our data provide new insights into the possible role of chIFITMs in early IBV infection.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #708422
Database	COVID19

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Article ; Online: Molecular cloning and characterisation of chicken IL-18 binding protein.

Gibson, Mark S / Steyn, Angela / Kealy, David / Kaspers, Bernd / Fife, Mark S

Developmental and comparative immunology

2020 Volume 114, Page(s) 103850

Abstract: The human IL-1 receptor family is comprised of 11 membrane bound or soluble receptors and the IL-18 binding protein (IL-18BP). These receptors are dispersed across seven genomic loci, with the majority at a single locus. Direct orthologues were ... ...

Abstract	The human IL-1 receptor family is comprised of 11 membrane bound or soluble receptors and the IL-18 binding protein (IL-18BP). These receptors are dispersed across seven genomic loci, with the majority at a single locus. Direct orthologues were identified in the chicken at conserved genomic loci; however, the IL-18BP remained absent from the first four builds of the chicken genome sequence. Subsequent assemblies identified the gene at a locus syntenic with mammals; however, these predicted sequences differed between genome builds and contained multiple errors. A partial IL-18BP-like sequence in the NCBI EST database was used to clone the full-length cDNA. A splice variant, which lacks the exon that encodes part of the signal peptide, was also cloned. Human IL-18BP is differentially spliced to produce a number of variants, which are all secreted. By contrast, the spliced chicken isoform was predicted to be intracellular, and we identified similar variants with the same exon missing in a limited number of divergent vertebrate species. Mammalian and viral IL-18BPs inhibit IL-18 activity by directly binding to this cytokine. Full-length and intracellular chicken IL-18BPs were equally effective at inhibiting IL-18-mediated IFN-γ release from an avian B-cell line. Analysis of the predicted chIL-18BP protein sequence revealed two crucial residues, which account for 50% of the binding affinity between human IL-18 and IL-18BP, are conserved in the chicken and a fowlpox-encoded homologue, fpv214. This suggests specific fowlpox viruses used in humans as a vaccine vector have the potential to dampen anti-viral host immune responses.
MeSH term(s)	Animals ; Avian Proteins/genetics ; Avian Proteins/metabolism ; B-Lymphocytes/immunology ; Cell Line ; Chickens/immunology ; Cloning, Molecular ; Fowlpox virus/genetics ; Fowlpox virus/metabolism ; Genetic Loci/genetics ; Genetic Vectors/genetics ; Host-Pathogen Interactions ; Immunomodulation ; Intercellular Signaling Peptides and Proteins/genetics ; Intercellular Signaling Peptides and Proteins/metabolism ; Interferon-gamma/metabolism ; Interleukin-18/metabolism ; Lymphocyte Activation ; Mammals ; Protein Binding ; Protein Isoforms/genetics ; Synteny ; Viral Proteins/genetics ; Viral Proteins/metabolism
Chemical Substances	Avian Proteins ; Intercellular Signaling Peptides and Proteins ; Interleukin-18 ; Protein Isoforms ; Viral Proteins ; interleukin-18 binding protein ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2020-09-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752411-0
ISSN	1879-0089 ; 0145-305X
ISSN (online)	1879-0089
ISSN	0145-305X
DOI	10.1016/j.dci.2020.103850
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Article ; Online: Epigenetic modification of the pentose phosphate pathway and the IGF-axis in women with gestational diabetes mellitus.

Steyn, Angela / Crowther, Nigel J / Norris, Shane A / Rabionet, Raquel / Estivill, Xavier / Ramsay, Michèle

Epigenomics

2019 Volume 11, Issue 12, Page(s) 1371–1385

Abstract: Aim: ...

Abstract	Aim:
MeSH term(s)	DNA Methylation ; Diabetes, Gestational/genetics ; Down-Regulation ; Epigenesis, Genetic ; Female ; Gene Expression Profiling ; Gestational Age ; Glucosephosphate Dehydrogenase/genetics ; Humans ; Infant, Newborn ; Insulin-Like Growth Factor Binding Proteins/genetics ; Pentose Phosphate Pathway ; Pregnancy ; Sequence Analysis, RNA
Chemical Substances	Insulin-Like Growth Factor Binding Proteins ; G6PD protein, human (EC 1.1.1.49) ; Glucosephosphate Dehydrogenase (EC 1.1.1.49)
Language	English
Publishing date	2019-10-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1750-192X
ISSN (online)	1750-192X
DOI	10.2217/epi-2018-0206
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Article: A Customizable Suite of Methods to Sequence and Annotate Cattle Antibodies.

Ramirez Valdez, Kristel / Nzau, Benjamin / Dorey-Robinson, Daniel / Jarman, Michael / Nyagwange, James / Schwartz, John C / Freimanis, Graham / Steyn, Angela W / Warimwe, George M / Morrison, Liam J / Mwangi, William / Charleston, Bryan / Bonnet-Di Placido, Marie / Hammond, John A

Vaccines

2023 Volume 11, Issue 6

Abstract: Studying the antibody response to infection or vaccination is essential for developing more effective vaccines and therapeutics. Advances in high-throughput antibody sequencing technologies and immunoinformatic tools now allow the fast and comprehensive ... ...

Abstract	Studying the antibody response to infection or vaccination is essential for developing more effective vaccines and therapeutics. Advances in high-throughput antibody sequencing technologies and immunoinformatic tools now allow the fast and comprehensive analysis of antibody repertoires at high resolution in any species. Here, we detail a flexible and customizable suite of methods from flow cytometry, single cell sorting, heavy and light chain amplification to antibody sequencing in cattle. These methods were used successfully, including adaptation to the 10x Genomics platform, to isolate native heavy-light chain pairs. When combined with the Ig-Sequence Multi-Species Annotation Tool, this suite represents a powerful toolkit for studying the cattle antibody response with high resolution and precision. Using three workflows, we processed 84, 96, and 8313 cattle B cells from which we sequenced 24, 31, and 4756 antibody heavy-light chain pairs, respectively. Each method has strengths and limitations in terms of the throughput, timeline, specialist equipment, and cost that are each discussed. Moreover, the principles outlined here can be applied to study antibody responses in other mammalian species.
Language	English
Publishing date	2023-06-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines11061099
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Article: Salicylic Acid Improves Growth and Physiological Attributes and Salt Tolerance Differentially in Two Bread Wheat Cultivars.

Abdi, Neila / Van Biljon, Angeline / Steyn, Chrisna / Labuschagne, Maryke Tine

Plants (Basel, Switzerland)

2022 Volume 11, Issue 14

Abstract: Abiotic constraints such as salinity stress reduce cereal production. Salicylic acid is an elicitor of abiotic stress tolerance in plants. The aim of this study was to investigate the effects of salicylic acid on two bread wheat cultivars (SST806 and ... ...

Abstract	Abiotic constraints such as salinity stress reduce cereal production. Salicylic acid is an elicitor of abiotic stress tolerance in plants. The aim of this study was to investigate the effects of salicylic acid on two bread wheat cultivars (SST806 and PAN3497) grown under salt stress (100 and 200 mM NaCl) in the presence and absence of 0.5 mM salicylic acid. The highest salt concentration (200 mM), in both PAN3497 and SST806, increased the days to germination and reduced the coleoptile and radicle dry weights. The shoot dry weight was reduced by 75 and 39%, root dry weight by 73 and 37%, spike number of both by 50%, spike weight by 73 and 54%, grain number by 62 and 15%, grain weight per spike by 80 and 45%, and 1000 grain weight by 9 and 29% for 200 and 100 mM NaCl, respectively. Salicylic acid in combination with 100 mM and 200 mM NaCl increased the shoot, root, and yield attributes. Salicylic acid increased the grain protein content, especially at 200 mM NaCl, and the increase was higher in SST806 than PAN3497. The macro-mineral concentration was markedly increased by an increase of NaCl. This was further increased by salicylic acid treatment for both SST806 and PAN3497. Regarding micro-minerals, Na was increased more than the other minerals in both cultivars. Mn, Zn, Fe, and Cu were increased under 100 mM and 200 Mm of salt, and salicylic acid application increased these elements further in both cultivars. These results suggested that salicylic acid application improved the salt tolerance of these two bread wheat cultivars.
Language	English
Publishing date	2022-07-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11141853
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Article ; Online: A Customizable Suite of Methods to Sequence and Annotate Cattle Antibodies

Kristel Ramirez Valdez / Benjamin Nzau / Daniel Dorey-Robinson / Michael Jarman / James Nyagwange / John C. Schwartz / Graham Freimanis / Angela W. Steyn / George M. Warimwe / Liam J. Morrison / William Mwangi / Bryan Charleston / Marie Bonnet-Di Placido / John A. Hammond

Vaccines, Vol 11, Iss 1099, p

2023 Volume 1099

Abstract	Studying the antibody response to infection or vaccination is essential for developing more effective vaccines and therapeutics. Advances in high-throughput antibody sequencing technologies and immunoinformatic tools now allow the fast and comprehensive analysis of antibody repertoires at high resolution in any species. Here, we detail a flexible and customizable suite of methods from flow cytometry, single cell sorting, heavy and light chain amplification to antibody sequencing in cattle. These methods were used successfully, including adaptation to the 10x Genomics platform, to isolate native heavy–light chain pairs. When combined with the Ig-Sequence Multi-Species Annotation Tool, this suite represents a powerful toolkit for studying the cattle antibody response with high resolution and precision. Using three workflows, we processed 84, 96, and 8313 cattle B cells from which we sequenced 24, 31, and 4756 antibody heavy–light chain pairs, respectively. Each method has strengths and limitations in terms of the throughput, timeline, specialist equipment, and cost that are each discussed. Moreover, the principles outlined here can be applied to study antibody responses in other mammalian species.
Keywords	antibody discovery ; IgMAT ; 10x Genomics ; antibody sequencing ; antibody repertoire ; Medicine ; R
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Feasibility of Using Endobronchial Ultrasound for a Complete Examination of the Thoracic Aorta: A Case Report of Acute Traumatic Aortic Injury.

Steyn, Johannes W / Rebel, Annette / Martin, Jeremiah / Mahan, Angela / Hassan, Zaki-Udin

Journal of cardiothoracic and vascular anesthesia

2017 Volume 31, Issue 4, Page(s) 1322–1325

MeSH term(s)	Aged ; Aorta, Thoracic/diagnostic imaging ; Aorta, Thoracic/surgery ; Aortic Rupture/diagnostic imaging ; Aortic Rupture/surgery ; Bronchoscopy/methods ; Feasibility Studies ; Female ; Humans ; Ultrasonography, Interventional/methods
Language	English
Publishing date	2017
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	1067317-9
ISSN	1532-8422 ; 1053-0770
ISSN (online)	1532-8422
ISSN	1053-0770
DOI	10.1053/j.jvca.2016.11.028
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Zs.MO 31: Show issues

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