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  1. Article ; Online: The Concept of Making On-Chip Thermal Cycler for RT-PCR Using Conjugate Heat Transfer in Diverging Microchannel.

    Duryodhan, V S / Singh, Shiv Govind / Agrawal, Amit

    Transactions of the Indian National Academy of Engineering : an international journal of engineering and technology

    2020  Volume 5, Issue 2, Page(s) 221–223

    Abstract: Covid-19 is pandemic to which the world is fighting. Various precautionary measures are being imposed all over the world which is affecting the routine life of an individual and also the economy worldwide. Although, a definite vaccine is still not known ... ...

    Abstract Covid-19 is pandemic to which the world is fighting. Various precautionary measures are being imposed all over the world which is affecting the routine life of an individual and also the economy worldwide. Although, a definite vaccine is still not known to medical science but they are able to distinguish Covid-19 from the other types of flu. Presently this is being done by detecting the SARS-CoV-2 virus using RT-PCR technique as recommended by the World Health Organization (WHO) (WHO, Geneva, 2020). Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a nucleic acid amplification test that converts the RNA into DNA and subsequently amplifies the specific DNA targets. This method was already being employed to detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) (Emery et al. in Emerg Infect Dis 10(2):311, 2004). The entire process of DNA amplification takes place in three steps: denaturation, annealing, and extension for which the sample is required to be maintained at constant temperatures of 95, 55 and 72 °C, respectively. This article introduces the technology to achieve a constant temperature which can be tweaked to develop on-chip RT-PCR.
    Language English
    Publishing date 2020-05-25
    Publishing country Singapore
    Document type Journal Article
    ISSN 2662-5423
    ISSN (online) 2662-5423
    DOI 10.1007/s41403-020-00108-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The Concept of Making On-Chip Thermal Cycler for RT-PCR Using Conjugate Heat Transfer in Diverging Microchannel

    Duryodhan, V. S. / Singh, Shiv Govind / Agrawal, Amit

    Transactions of the Indian National Academy of Engineering

    Abstract: Covid-19 is pandemic to which the world is fighting Various precautionary measures are being imposed all over the world which is affecting the routine life of an individual and also the economy worldwide Although, a definite vaccine is still not known to ...

    Abstract Covid-19 is pandemic to which the world is fighting Various precautionary measures are being imposed all over the world which is affecting the routine life of an individual and also the economy worldwide Although, a definite vaccine is still not known to medical science but they are able to distinguish Covid-19 from the other types of flu Presently this is being done by detecting the SARS-CoV-2 virus using RT-PCR technique as recommended by the World Health Organization (WHO) (WHO, Geneva, 2020) Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a nucleic acid amplification test that converts the RNA into DNA and subsequently amplifies the specific DNA targets This method was already being employed to detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) (Emery et al in Emerg Infect Dis 10(2):311, 2004) The entire process of DNA amplification takes place in three steps: denaturation, annealing, and extension for which the sample is required to be maintained at constant temperatures of 95, 55 and 72 °C, respectively This article introduces the technology to achieve a constant temperature which can be tweaked to develop on-chip RT-PCR
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #361380
    Database COVID19

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  3. Article ; Online: A simple and novel way of maintaining constant wall temperature in microdevices.

    Duryodhan, V S / Singh, Abhimanyu / Singh, Shiv Govind / Agrawal, Amit

    Scientific reports

    2016  Volume 6, Page(s) 18230

    Abstract: ... and input conditions (mass flow rate: 4.17 × 10(-5) -9.17 × 10(-5) kg/s, heat flux: 2.4-9.6 W/cm(2 ...

    Abstract Constant wall temperature /homogeneity in wall temperature is the need of various lab-on-chip devices employed in biological and chemical investigations. However method to maintain this condition does not seem to be available. In this work, a novel and simple way of maintaining constant wall temperature is proposed. A diverging microchannel along with conjugate effects is utilized towards this end. Both measurements and three dimensional numerical simulations are undertaken to prove the design. The investigation has been carried out over a large parameter range (divergence angle: 1-8°; length: 10-30 mm; depth: 86-200 μm; solid-to-fluid thickness ratio: 1.5-4.0, and solid-to-fluid thermal conductivity ratio: 27-646) and input conditions (mass flow rate: 4.17 × 10(-5) -9.17 × 10(-5) kg/s, heat flux: 2.4-9.6 W/cm(2)) which helped in establishing the finding. It is observed that a nearly constant wall temperature condition can be achieved over a large parameter range investigated. A model to arrive at the design parameter values is also proposed. The method is further demonstrated for series of microchannels where we successfully maintain each station at different temperature within ± 1 °C. The finding is therefore significant and can be employed in both single and multi-stage processes such as PCR requiring different constant wall temperature with a fine resolution.
    Language English
    Publishing date 2016-01-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/srep18230
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Three-Dimensional Numerical Study of Conjugate Heat Transfer in Diverging Microchannel

    V S Duryodhan, Abhimanyu Singh, S G Singh and Amit

    Proceedings of Indian National Science Academy, Vol 82, Iss 2, Pp 321-

    2016  Volume 328

    Abstract: Three-Dimensional Numerical Study of Conjugate Heat Transfer in Diverging ... ...

    Abstract Three-Dimensional Numerical Study of Conjugate Heat Transfer in Diverging Microchannel
    Keywords Wall conduction ; diverging microchannel ; heat flux gradient ; Science ; Q
    Language English
    Publishing date 2016-06-01T00:00:00Z
    Publisher Indian National Science Academy
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  5. Article: Enhancing Miscible Fluid Mixing by Introducing Pseudo Turbulent Flow in Golden Ratio Spiral Microchannel

    Kommalapati, Sahil / Agrawal, Amit / Duryodhan, Vijay S

    Industrial & engineering chemistry process design and development. 2019 Oct. 17, v. 59, no. 9

    2019  

    Abstract: Mixing of fluids has numerous applications in laboratory processes, and several different designs have been proposed in the literature to achieve this objective at the microscale. In this work, the mixing efficiency for various spiral microchannels is ... ...

    Abstract Mixing of fluids has numerous applications in laboratory processes, and several different designs have been proposed in the literature to achieve this objective at the microscale. In this work, the mixing efficiency for various spiral microchannels is computed numerically. We also propose a new geometry, a Golden ratio spiral microchannel, which is found to have a higher mixing efficiency as compared to four other types of spiral designs (logarithmic, Archimedean, hyperbolic, Euler) tested here. A Golden ratio spiral microchannel involves a stepwise varying radius of curvature. The larger mixing efficiency of Golden ratio spiral microchannel is owing to pseudo turbulence, created through a superposition of different vortex formations. The mixing efficiency over a large range of Reynolds number (5 ≤ Re ≤ 100) is studied to generalize the findings and the results apply till at least Re = 1000. The present results will be useful in the design of industrial micromixers for various applications.
    Keywords Reynolds number ; mixing ; turbulent flow
    Language English
    Dates of publication 2019-1017
    Size p. 3784-3793.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1484436-9
    ISSN 1520-5045 ; 0888-5885
    ISSN (online) 1520-5045
    ISSN 0888-5885
    DOI 10.1021/acs.iecr.9b05276
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Synthesis of Dy doped Co–Zn ferrite by sol–gel auto combustion method and its characterization

    Kulal, Shivaji R / Khetre, Sanjay S / Jagdale, Pramod N / Gurame, Vashishtha M / Waghmode, Duryodhan P / Kolekar, Govind B / Sabale, Sandip R / Bamane, Sambhaji R

    Materials letters. 2012 Oct. 1, v. 84

    2012  

    Abstract: Dysprosium doped Co–Zn ferrites were synthesized by sol–gel auto combustion method. These synthesized compounds were characterized by using XRD, SEM, FTIR, EDS, TGA-DTA and TEM. TGA weight loss in the region of 100–200°C and this loss is due to ... ...

    Abstract Dysprosium doped Co–Zn ferrites were synthesized by sol–gel auto combustion method. These synthesized compounds were characterized by using XRD, SEM, FTIR, EDS, TGA-DTA and TEM. TGA weight loss in the region of 100–200°C and this loss is due to autocatalytic oxidation reduction reactions of nitrates with glycine. The composition Co₀.₅Zn₀.₅Fe₂₋ₓDyₓO₄ (x=0.075) shows fibrous reticulated mesh like morphology. The average grain size increases with increasing the Dy content in the Co–Zn ferrite. The XRD study of Dy doped Co–Zn ferrite shows in Cubic spinel phase. Particle size obtained from TEM analysis was found to be 30–40nm. The FTIR spectra showed the characteristic of two strong absorption bands at 560cm⁻¹ corresponds to intrinsic stretching vibrations of the metal at the tetrahedral site and lowest band is observed at 455cm⁻¹ corresponds to octahedral site.
    Keywords Fourier transform infrared spectroscopy ; X-ray diffraction ; absorption ; combustion ; dysprosium ; nitrates ; particle size ; scanning electron microscopy ; transmission electron microscopy ; weight loss
    Language English
    Dates of publication 2012-1001
    Size p. 169-172.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1491964-3
    ISSN 1873-4979 ; 0167-577X
    ISSN (online) 1873-4979
    ISSN 0167-577X
    DOI 10.1016/j.matlet.2012.06.025
    Database NAL-Catalogue (AGRICOLA)

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