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Article ; Online: Seminal plasma proteins of Atlantic halibut (Hippoglossus hippoglossus L.).

Mommens, Maren / Wojtczak, Mariola / Ciereszko, Andrzej / Babiak, Igor

2008 Volume 34, Issue 4, Page(s) 349–355

Abstract: ... 367.4 U l(-l). A high correlation between anti-proteolytic activity and protein concentration (r = 0 ...

Abstract	Protein content and properties in the seminal plasma of Atlantic halibut (Hippoglossus hippoglossus) were assayed using spectrophotometric and electrophoretic methods. The protein concentration ranged from 6.4 +/- 3.1 to 19.4 +/- 3.4 mg ml(-1) and anti-proteolytic activity from 585.2 +/- 104.6 to 2912.4 +/- 367.4 U l(-l). A high correlation between anti-proteolytic activity and protein concentration (r = 0.95), and between sperm concentration and osmolality was found (r = 0.92). There was a significant decrease in anti-proteolytic activity from the first to the second sampling, but not in protein concentration. Anti-proteolytic activity and protein concentration were significantly affected by variations in individual males. Electrophoresis revealed four anti-proteolytic bands and individual differences in bands of proteolytic activity, which were subsequently characterized as metalloproteases and serine proteases.
MeSH term(s)	Animals ; Benzamidines/pharmacology ; Calcium Chloride/pharmacology ; Chelating Agents/pharmacology ; Edetic Acid/pharmacology ; Flounder/metabolism ; Flounder/physiology ; Gelatinases/metabolism ; Male ; Osmolar Concentration ; Semen/drug effects ; Semen/enzymology ; Semen/metabolism ; Seminal Plasma Proteins/metabolism ; Serine Proteinase Inhibitors/pharmacology ; Sperm Count
Chemical Substances	Benzamidines ; Chelating Agents ; Seminal Plasma Proteins ; Serine Proteinase Inhibitors ; Edetic Acid (9G34HU7RV0) ; Gelatinases (EC 3.4.24.-) ; benzamidine (KUE3ZY3J1F) ; Calcium Chloride (M4I0D6VV5M)
Language	English
Publishing date	2008-12
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	292907-7
ISSN	1573-5168 ; 0920-1742
ISSN (online)	1573-5168
ISSN	0920-1742
DOI	10.1007/s10695-007-9194-x
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Article: Protective role of L-methionine against free radical damage of rat brain synaptosomes.

Slyshenkov, Vyacheslav S / Shevalye, Anna A / Liopo, Anton V / Wojtczak, Lech

Acta biochimica Polonica

2002 Volume 49, Issue 4, Page(s) 907–916

Abstract: ... glutathione transferase. Preincubation of the synaptosomal/mitochondrial fraction with 0.5 or 1.0 mM L-methionine ... The importance of L-methionine in antioxidant protection is discussed. ...

Abstract	Incubation of rat brain synaptosomal/mitochondrial fraction with tert-butylhydroperoxide resulted in accumulation of the lipid peroxidation product, conjugated dienes, damage of the synaptosomal membrane as evidenced by leakage of lactate dehydrogenase, and decrease of the total content of glutathione and of the GSH/GSSG ratio. This treatment also produced a considerable decrease of the ouabain-sensitive ATPase activity and a much smaller diminution of the activities of glutathione reductase and glutathione transferase. Preincubation of the synaptosomal/mitochondrial fraction with 0.5 or 1.0 mM L-methionine significantly protected against lipid peroxidation, membrane damage and changes in the glutathione system produced by low (1 mM) concentrations of tert-butylhydroperoxide and completely prevented inactivation of ouabain-sensitive ATPase, glutathione reductase and glutathione transferase by such treatment. The importance of L-methionine in antioxidant protection is discussed.
MeSH term(s)	Animals ; Brain/cytology ; Brain/drug effects ; Brain/metabolism ; Enzyme Activation/drug effects ; Free Radicals/metabolism ; Glutathione/metabolism ; Glutathione Peroxidase/metabolism ; Glutathione Reductase/metabolism ; Glutathione Transferase/metabolism ; Methionine/pharmacology ; Mitochondria/drug effects ; Mitochondria/enzymology ; Mitochondria/metabolism ; Oxidation-Reduction/drug effects ; Oxidative Stress/drug effects ; Rats ; Rats, Inbred Strains ; Synaptosomes/drug effects ; Synaptosomes/enzymology ; Synaptosomes/metabolism ; tert-Butylhydroperoxide/pharmacology
Chemical Substances	Free Radicals ; tert-Butylhydroperoxide (955VYL842B) ; Methionine (AE28F7PNPL) ; Glutathione Peroxidase (EC 1.11.1.9) ; Glutathione Reductase (EC 1.8.1.7) ; Glutathione Transferase (EC 2.5.1.18) ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2002
Publishing country	Poland
Document type	Journal Article
ZDB-ID	595762-x
ISSN	1734-154X ; 0001-527X
ISSN (online)	1734-154X
ISSN	0001-527X
DOI	024904907
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Article: Półwiecze :chemiosmotycznej" koncepcji sprzezenia energetycznego. Z historii biochemii.

Wojtczak, Lech

Postepy biochemii

2014 Volume 60, Issue 1, Page(s) 33–38

Abstract: History of the formulation of the "chemiosmotic" energy coupling concept of oxidative phosphorylation and photophosphorylation is described. A short CV of its author Peter Mitchell is also presented. ...

Title translation	Half century of the concept of "chemiosmotic" energy coupling ,.
Abstract	History of the formulation of the "chemiosmotic" energy coupling concept of oxidative phosphorylation and photophosphorylation is described. A short CV of its author Peter Mitchell is also presented.
MeSH term(s)	Biochemistry/history ; Energy Metabolism/physiology ; History, 20th Century ; Mitochondria/metabolism ; Oxidative Phosphorylation ; Photophosphorylation ; Poland
Language	Polish
Publishing date	2014
Publishing country	Poland
Document type	Biography ; English Abstract ; Historical Article ; Journal Article ; Portraits ; Review
ZDB-ID	414019-9
ISSN	0032-5422
ISSN	0032-5422
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Article: Seminal plasma proteins of Atlantic halibut (Hippoglossus hippoglossus L.)

Mommens, Maren / Wojtczak, Mariola / Ciereszko, Andrzej / Babiak, Igor

Fish physiology and biochemistry. 2008 Dec., v. 34, no. 4

2008

Abstract: Protein content and properties in the seminal plasma of Atlantic halibut (Hippoglossus hippoglossus) were assayed using spectrophotometric and electrophoretic methods. The protein concentration ranged from 6.4 ± 3.1 to 19.4 ± 3.4 mg ml⁻¹ and anti- ... ...

Abstract	Protein content and properties in the seminal plasma of Atlantic halibut (Hippoglossus hippoglossus) were assayed using spectrophotometric and electrophoretic methods. The protein concentration ranged from 6.4 ± 3.1 to 19.4 ± 3.4 mg ml⁻¹ and anti-proteolytic activity from 585.2 ± 104.6 to 2912.4 ± 367.4 U l⁻l. A high correlation between anti-proteolytic activity and protein concentration (r = 0.95), and between sperm concentration and osmolality was found (r = 0.92). There was a significant decrease in anti-proteolytic activity from the first to the second sampling, but not in protein concentration. Anti-proteolytic activity and protein concentration were significantly affected by variations in individual males. Electrophoresis revealed four anti-proteolytic bands and individual differences in bands of proteolytic activity, which were subsequently characterized as metalloproteases and serine proteases.
Keywords	Hippoglossus hippoglossus ; blood proteins ; electrophoresis ; males ; metalloproteinases ; osmolality ; protein content ; seminal plasma ; serine proteinases ; spermatozoa
Language	English
Dates of publication	2008-12
Size	p. 349-355.
Publisher	Springer Netherlands
Publishing place	Dordrecht
Document type	Article
ZDB-ID	292907-7
ISSN	1573-5168 ; 0920-1742
ISSN (online)	1573-5168
ISSN	0920-1742
DOI	10.1007/s10695-007-9194-x
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: An Update on Isolation of Functional Mitochondria from Cells for Bioenergetics Studies.

Lebiedzinska-Arciszewska, Magdalena / Wojtczak, Lech / Wieckowski, Mariusz R

Methods in molecular biology (Clifton, N.J.)

2021 Volume 2310, Page(s) 79–89

Abstract: Mitochondria are the organelles where the most fundamental processes of energy transformation within the cell are located. They are also involved in several processes like apoptosis and autophagy, reactive oxygen species formation, and calcium signaling, ...

Abstract	Mitochondria are the organelles where the most fundamental processes of energy transformation within the cell are located. They are also involved in several processes like apoptosis and autophagy, reactive oxygen species formation, and calcium signaling, which are crucial for proper cell functioning. In addition, mitochondrial genome hosts genes encoding important proteins incorporated in respiratory chain complexes and indispensable for the oxidative phosphorylation. Studying isolated mitochondria is, therefore, crucial for better understanding of cell physiology. The presented protocol describes a relatively simple and handy method for crude mitochondrial fraction isolation from different mammalian cell lines. It includes mechanical cells disruption (homogenization) and differential centrifugation. In addition, this chapter presents two basic ways to assess mitochondrial functionality: by measuring mitochondrial inner membrane potential and coupled respiration.
MeSH term(s)	Animals ; Cell Fractionation ; Cell Respiration ; Centrifugation ; Energy Metabolism ; Fluorometry ; HeLa Cells ; Humans ; Jurkat Cells ; Membrane Potential, Mitochondrial ; Mice ; Mitochondria/metabolism ; Oxygen Consumption ; Rats
Language	English
Publishing date	2021-06-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-1433-4_7
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Article: Effects of Age on Cortical Tracking of Word-Level Features of Continuous Competing Speech.

Mesik, Juraj / Ray, Lucia / Wojtczak, Magdalena

Frontiers in neuroscience

2021 Volume 15, Page(s) 635126

Abstract: Speech-in-noise comprehension difficulties are common among the elderly population, yet traditional objective measures of speech perception are largely insensitive to this deficit, particularly in the absence of clinical hearing loss. In recent years, a ... ...

Abstract	Speech-in-noise comprehension difficulties are common among the elderly population, yet traditional objective measures of speech perception are largely insensitive to this deficit, particularly in the absence of clinical hearing loss. In recent years, a growing body of research in young normal-hearing adults has demonstrated that high-level features related to speech semantics and lexical predictability elicit strong centro-parietal negativity in the EEG signal around 400 ms following the word onset. Here we investigate effects of age on cortical tracking of these word-level features within a two-talker speech mixture, and their relationship with self-reported difficulties with speech-in-noise understanding. While undergoing EEG recordings, younger and older adult participants listened to a continuous narrative story in the presence of a distractor story. We then utilized forward encoding models to estimate cortical tracking of four speech features: (1) word onsets, (2) "semantic" dissimilarity of each word relative to the preceding context, (3) lexical surprisal for each word, and (4) overall word audibility. Our results revealed robust tracking of all features for attended speech, with surprisal and word audibility showing significantly stronger contributions to neural activity than dissimilarity. Additionally, older adults exhibited significantly stronger tracking of word-level features than younger adults, especially over frontal electrode sites, potentially reflecting increased listening effort. Finally, neuro-behavioral analyses revealed trends of a negative relationship between subjective speech-in-noise perception difficulties and the model goodness-of-fit for attended speech, as well as a positive relationship between task performance and the goodness-of-fit, indicating behavioral relevance of these measures. Together, our results demonstrate the utility of modeling cortical responses to multi-talker speech using complex, word-level features and the potential for their use to study changes in speech processing due to aging and hearing loss.
Language	English
Publishing date	2021-04-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2411902-7
ISSN	1662-453X ; 1662-4548
ISSN (online)	1662-453X
ISSN	1662-4548
DOI	10.3389/fnins.2021.635126
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Article ; Online: Maria und das Alte Testament, red. M. Hauke, Regensburg 2015

Adam Wojtczak

Seminare, Vol 37, Iss

2016 Volume 2

Keywords	Education ; L ; Social Sciences ; H
Language	German
Publishing date	2016-05-01T00:00:00Z
Publisher	The Francis de Sales Scientific Society (TNFS.PL)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Britton Chance (1913-2010)--od zeglarstwa do biofizyki i biochemii i z powrotem. Z historii biochemii.

Wojtczak, Lech

Postepy biochemii

2011 Volume 57, Issue 1, Page(s) 9–12

Abstract: A short CV of Britton Chance, an outstanding American biophysicist and biochemists, who has passed away in November 2010, is presented. Chance invented and applied in biochemical research fast recording of difference spectra during chemical reactions. ... ...

Title translation	Britton Chance (1913-2010)--from sailing to biophysics and biochemistry and back.
Abstract	A short CV of Britton Chance, an outstanding American biophysicist and biochemists, who has passed away in November 2010, is presented. Chance invented and applied in biochemical research fast recording of difference spectra during chemical reactions. Due to this technique he discovered and studied formation of enzyme-substrate complexes as exemplified by the peroxidase reaction. He also described spectral changes of electron carriers of mitochondrial respiratory chain that accompany transition from the resting to the active (phosphorylating) states. Applying vibrating platinum electrode, he recorded changes in the rate of oxygen uptake by isolated mitochondria in resting and phosphorylating states and defined the so-called respiratory states, called after his name. He was the first to describe formation of reactive oxygen species by mitochondria. Britton Chance was a devoted yachtsman and won a gold medal for the United States in sailing at the 1952 Olympic Games in Helsinki.
MeSH term(s)	Biophysics/history ; Famous Persons ; History, 20th Century ; History, 21st Century ; Immunoenzyme Techniques/history ; Mitochondria/metabolism ; Oxygen Consumption ; Reactive Oxygen Species/metabolism ; Sports/history ; United States
Chemical Substances	Reactive Oxygen Species
Language	Polish
Publishing date	2011
Publishing country	Poland
Document type	Biography ; English Abstract ; Historical Article ; Journal Article ; Portraits
ZDB-ID	414019-9
ISSN	0032-5422
ISSN	0032-5422
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Article ; Online: The age threshold of the 8th edition AJCC classification is useful for indicating patients with aggressive papillary thyroid cancer in clinical practice.

Kaliszewski, Krzysztof / Diakowska, Dorota / Nowak, Łukasz / Wojtczak, Beata / Rudnicki, Jerzy

BMC cancer

2020 Volume 20, Issue 1, Page(s) 1166

Abstract: Background: Papillary thyroid cancer (PTC) is unique among cancers in that patient age is a consideration in staging. One of the most important modifications in the 8th Edition of the American Joint Committee on Cancer (AJCC) classification is to ... ...

Abstract	Background: Papillary thyroid cancer (PTC) is unique among cancers in that patient age is a consideration in staging. One of the most important modifications in the 8th Edition of the American Joint Committee on Cancer (AJCC) classification is to increase the age cut off for risk stratification in PTC from 45 to 55 years. However, whether this cut off is useful in clinical practice remains controversial. In the present study, we assessed how well this new age threshold stratifies patients with aggressive PTC. Methods: We retrospectively analyzed the clinicopathological features and overall survival rate of patients with PTC admitted to and surgically treated at a single surgical center. The study protocol was divided into two series. In each series all patients (n = 523) were divided in 2 groups according to age cut off. In the first series (cut off 45) patients < 45 (n = 193) vs. ≥45 (n = 330) were compared, and in the second series (cut off 55) patients < 55 (n = 306) vs. ≥55 (n = 217) were compared. Results: The rate of the prevalence of locally advanced disease (pT3 and pT4) was significantly higher in the patients above 55 years old than in those below 55 years old (p = 0.013). No significant differences were found for this parameter in series with cut off point 45 years old. A significantly higher risk of locally advanced disease T3 + T4 (OR = 4.87) and presence of LNM (N1) (OR = 3.78) was observed in ≥45 years old group (p = 0.021 and p < 0.0001, respectively). More expressive results were found for the patients ≥55 years old group, where the risk of locally advanced disease (T3 + T4) was higher (OR = 5.21) and LNM presence was OR = 4.76 (p < 0.001 and p < 0.0001, respectively). None of the patients below 55 years old showed distant metastasis, but 19 patients above 55 years old showed M1 (p < 0.0001). In older patients group (≥55 years old) we observed deaths related thyroid cancer in 11 individuals. Conclusions: The age cut off of 55 years old for risk stratification proposed by the 8th Edition of AJCC effectively stratifies PTC patients with a poor prognosis, indicating it is likely to be useful in clinical practice.
MeSH term(s)	Age Factors ; Female ; Humans ; Male ; Middle Aged ; Periodicals as Topic/classification ; Retrospective Studies ; Thyroid Cancer, Papillary/epidemiology
Language	English
Publishing date	2020-11-30
Publishing country	England
Document type	Journal Article
ISSN	1471-2407
ISSN (online)	1471-2407
DOI	10.1186/s12885-020-07636-0
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Article: From mitochondrial large amplitude swelling to the permeability transition - a short historic overview.

Wojtczak, Lech / Więckowski, Mariusz R

Postepy biochemii

2016 Volume 62, Issue 3, Page(s) 298–302

Abstract: An outline of studies on the mitochondrial large conductance permeability pore is presented starting from the early observations in the 1950s on the large amplitude mitochondrial swelling, through the concept of the permeability transition and various ... ...

Title translation	Od pęcznienia mitochondriów po koncepcję transformacji przepuszczalnościowej - krótki zarys historyczny.
Abstract	An outline of studies on the mitochondrial large conductance permeability pore is presented starting from the early observations in the 1950s on the large amplitude mitochondrial swelling, through the concept of the permeability transition and various theories on the structure of the related permeability transition pore, up to its present identification as a part of mitochondrial (F
Language	English
Publishing date	2016
Publishing country	Poland
Document type	Journal Article ; Review
ZDB-ID	414019-9
ISSN	0032-5422
ISSN	0032-5422
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