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  1. Article ; Online: Visualizing Effector Triggered Immunity in Response to Pore-Forming Toxins by Live-Cell Imaging.

    Chow, Seong H / Naderer, Thomas

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2523, Page(s) 239–252

    Abstract: The NLRP3 inflammasome senses the activity of pore-forming toxins secreted by Staphylococcus aureus. The bacterial toxins compromise plasma membrane integrity which activates the NLRP3 inflammasome to induce host pore-forming proteins and cellular ... ...

    Abstract The NLRP3 inflammasome senses the activity of pore-forming toxins secreted by Staphylococcus aureus. The bacterial toxins compromise plasma membrane integrity which activates the NLRP3 inflammasome to induce host pore-forming proteins and cellular suicide, termed pyroptosis. Host cell death rates are routinely determined at pre-defined time points and on whole cell populations. To capture the dynamic interactions between bacterial pore-forming toxins and host cell death factors, we have applied live-cell imaging techniques capable of analyzing single cell events in real time. Here, we describe methods using live-cell imaging to determine the host responses, such as plasma membrane integrity, mitochondrial health, and apoptotic caspases, towards pore-forming toxins.
    MeSH term(s) Bacterial Toxins/metabolism ; Cell Survival ; Humans ; Inflammasomes/metabolism ; Macrophages/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Pyroptosis ; Signal Transduction
    Chemical Substances Bacterial Toxins ; Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein
    Language English
    Publishing date 2022-06-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2449-4_15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Towards re-purposing BH3-mimetics in Legionella and viral infections.

    Naderer, Thomas

    Expert review of anti-infective therapy

    2017  Volume 15, Issue 12, Page(s) 1071–1073

    MeSH term(s) Animals ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Antiviral Agents/chemistry ; Antiviral Agents/pharmacology ; Humans ; Legionellosis/drug therapy ; Peptide Fragments/chemistry ; Peptidomimetics/chemistry ; Peptidomimetics/pharmacology ; Proto-Oncogene Proteins/chemistry ; Virus Diseases/drug therapy
    Chemical Substances Anti-Bacterial Agents ; Antiviral Agents ; Bax protein (53-86) ; Peptide Fragments ; Peptidomimetics ; Proto-Oncogene Proteins
    Language English
    Publishing date 2017-11-29
    Publishing country England
    Document type Editorial ; Research Support, Non-U.S. Gov't
    ZDB-ID 2181279-2
    ISSN 1744-8336 ; 1478-7210
    ISSN (online) 1744-8336
    ISSN 1478-7210
    DOI 10.1080/14787210.2017.1409621
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  3. Article ; Online: The role of monocausal versus multicausal explanations of suicide in suicide reporting: A randomized controlled trial.

    Till, Benedikt / Arendt, Florian / Kirchner, Stefanie / Naderer, Brigitte / Niederkrotenthaler, Thomas

    Suicide & life-threatening behavior

    2023  Volume 53, Issue 6, Page(s) 1063–1075

    Abstract: Background: Media guidelines for reporting on suicide recommend that journalists should avoid monocausal explanations of suicide, but it is unclear if media items with monocausal explanations elicit different effects as compared to multicausal ... ...

    Abstract Background: Media guidelines for reporting on suicide recommend that journalists should avoid monocausal explanations of suicide, but it is unclear if media items with monocausal explanations elicit different effects as compared to multicausal portrayals.
    Method: Using a web-based randomized controlled trial (n = 969), we tested five versions of a news article about the suicide of a teenage girl with varying portrayals of reasons for the suicide: (1) bullying as the sole (external) factor (i.e., monocausal), (2) several external social factors, (3) a combination of internal and external factors, (4) a combination of internal and external factors along with a focus on suicide prevention, or (5) no reason for the suicide (control group). We measured perceptions about the cause of suicide, attitudes toward suicide and suicide prevention, and identification with the suicidal protagonist with questionnaires.
    Results: Readers of articles that portrayed suicide as being caused by one specific reason or exclusively social factors tended to adopt these misconceptions. Identification with the suicidal protagonist did not vary between interventions groups, but was lower in the control group.
    Conclusion: Highlighting the multifactorial etiology of suicide in news articles may help to avoid the misconception that suicide is a monocausal issue.
    MeSH term(s) Female ; Adolescent ; Humans ; Mass Media ; Suicide ; Suicide Prevention ; Suicidal Ideation ; Bullying
    Language English
    Publishing date 2023-10-12
    Publishing country England
    Document type Randomized Controlled Trial ; Journal Article
    ZDB-ID 750058-0
    ISSN 1943-278X ; 0047-4592 ; 0363-0234
    ISSN (online) 1943-278X
    ISSN 0047-4592 ; 0363-0234
    DOI 10.1111/sltb.13007
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    Zs.B 1729: Show issues Location:
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  4. Article ; Online: Editorial: Understanding the effects of metabolites and trace minerals on microbes during infection.

    Naderer, Thomas / Mu, Andre / Monteith, Andrew J / Wong Fok Lung, Tania

    Frontiers in cellular and infection microbiology

    2023  Volume 13, Page(s) 1276271

    MeSH term(s) Trace Elements ; Infections ; Bacteria
    Chemical Substances Trace Elements
    Language English
    Publishing date 2023-08-30
    Publishing country Switzerland
    Document type Editorial ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2023.1276271
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  5. Article: Transcriptomic interplay between

    Kho, Zhi Ying / Azad, Mohammad Abul Kalam / Zhu, Yan / Han, Mei-Ling / Zhou, Qi Tony / Velkov, Tony / Naderer, Thomas / Li, Jian

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Optimization of antibiotic therapy has been hindered by our dearth of understanding on the mechanism of the host-pathogen-drug interactions. Here, we employed dual RNA-sequencing to examine transcriptomic perturbations in response to polymyxin B in a co- ... ...

    Abstract Optimization of antibiotic therapy has been hindered by our dearth of understanding on the mechanism of the host-pathogen-drug interactions. Here, we employed dual RNA-sequencing to examine transcriptomic perturbations in response to polymyxin B in a co-culture infection model of
    Importance: In the context of the development of bacterial resistance during the course of antibiotic therapy, the role of macrophages in shaping bacterial response to antibiotic killing remains enigmatic. Herein we employed dual RNA-sequencing and an
    Language English
    Publishing date 2024-01-23
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.01.23.576770
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  6. Article ; Online: Low-Fluorescence Starter for Optical 3D Lithography of Sub-40 nm Structures.

    Gvindzhiliia, Georgii / Sivun, Dmitry / Naderer, Christoph / Jacak, Jaroslaw / Klar, Thomas A

    ACS applied optical materials

    2023  Volume 1, Issue 5, Page(s) 945–951

    Abstract: Stimulated emission depletion (STED) has been used to break the diffraction limit in fluorescence microscopy. Inspired by this success, similar methods were used to reduce the structure size in three-dimensional, subdiffractional optical lithography. So ... ...

    Abstract Stimulated emission depletion (STED) has been used to break the diffraction limit in fluorescence microscopy. Inspired by this success, similar methods were used to reduce the structure size in three-dimensional, subdiffractional optical lithography. So far, only a very limited number of radical polymerization starters proved to be suitable for STED-inspired lithography. In this contribution, we introduce the starter Michler's ethyl ketone (MEK), which has not been used so far for STED-inspired lithography. In contrast to the commonly used 7-diethylamino-3-thenoylcoumarin (DETC), nanostructures written with MEK show low autofluorescence in the visible range. Therefore, MEK is promising for being used as a starter for protein or cell scaffolds in physiological research because the autofluorescence of DETC so far excluded the use of the green emission channel in multicolor fluorescence or confocal microscopy. In turn, because of the weak transitions of MEK in the visible spectrum, STED, in its original sense, cannot be applied to deplete MEK in the outer rim of the point spread function. However, a 660 nm laser can be used for depletion because this wavelength is well within the absorption spectrum of transient states, possibly of triplet states. We show that polymerization can be fully stopped by applying transient state absorption at 660 nm and that structure sizes down to approx. 40 nm in the lateral and axial directions can be achieved, which means 1/20 of the optical wavelength used for writing.
    Language English
    Publishing date 2023-05-12
    Publishing country United States
    Document type Journal Article
    ISSN 2771-9855
    ISSN (online) 2771-9855
    DOI 10.1021/acsaom.3c00031
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  7. Article ; Online: Bacterial outer membrane vesicles and host cell death signaling.

    Dhital, Subhash / Deo, Pankaj / Stuart, Isabella / Naderer, Thomas

    Trends in microbiology

    2021  Volume 29, Issue 12, Page(s) 1106–1116

    Abstract: The programmed cell death pathways of pyroptosis and apoptosis protect mammals from infections. The activation of host cell death signaling depends on cell surface and cytosolic receptors that bind bacterial molecules or sense their activity. The ... ...

    Abstract The programmed cell death pathways of pyroptosis and apoptosis protect mammals from infections. The activation of host cell death signaling depends on cell surface and cytosolic receptors that bind bacterial molecules or sense their activity. The formation of cytosolic protein complexes, such as the inflammasome and apoptosome, activates caspases, pore-forming proteins, and inflammatory cytokines. These pathways respond to bacteria and their released membrane vesicles. Outer membrane vesicles (OMVs) that emerge from the outer membrane of Gram-negative bacteria deliver a range of bacterial molecules, including lipids, proteins, polysaccharides and nucleic acids to host cells. Recent findings describe how OMV-associated molecules activate pyroptosis, apoptosis, and other inflammatory pathways. We discuss here how OMV-associated molecules are sensed by the immune system and how this contributes to infections and inflammatory diseases.
    MeSH term(s) Animals ; Apoptosis ; Bacterial Outer Membrane ; Bacterial Outer Membrane Proteins/metabolism ; Cell Membrane/metabolism ; Extracellular Vesicles/metabolism ; Gram-Negative Bacteria/metabolism ; Inflammasomes/metabolism ; Mammals
    Chemical Substances Bacterial Outer Membrane Proteins ; Inflammasomes
    Language English
    Publishing date 2021-05-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2021.04.003
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    Zs.A 3738: Show issues Location:
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  8. Article ; Online: Central metabolic interactions of immune cells and microbes: prospects for defeating infections.

    Traven, Ana / Naderer, Thomas

    EMBO reports

    2019  Volume 20, Issue 7, Page(s) e47995

    Abstract: Antimicrobial drug resistance is threatening to take us to the "pre-antibiotic era", where people are dying from preventable and treatable diseases and the risk of hospital-associated infections compromises the success of surgery and cancer treatments. ... ...

    Abstract Antimicrobial drug resistance is threatening to take us to the "pre-antibiotic era", where people are dying from preventable and treatable diseases and the risk of hospital-associated infections compromises the success of surgery and cancer treatments. Development of new antibiotics is slow, and alternative approaches to control infections have emerged based on insights into metabolic pathways in host-microbe interactions. Central carbon metabolism of immune cells is pivotal in mounting an effective response to invading pathogens, not only to meet energy requirements, but to directly activate antimicrobial responses. Microbes are not passive players here-they remodel their metabolism to survive and grow in host environments. Sometimes, microbes might even benefit from the metabolic reprogramming of immune cells, and pathogens such as Candida albicans, Salmonella Typhimurium and Staphylococcus aureus can compete with activated host cells for sugars that are needed for essential metabolic pathways linked to inflammatory processes. Here, we discuss how metabolic interactions between innate immune cells and microbes determine their survival during infection, and ways in which metabolism could be manipulated as a therapeutic strategy.
    MeSH term(s) Animals ; Communicable Diseases/immunology ; Communicable Diseases/metabolism ; Communicable Diseases/microbiology ; Host-Pathogen Interactions ; Humans ; Immunity, Innate ; Macrophages/immunology ; Macrophages/metabolism ; Macrophages/microbiology ; Metabolome
    Language English
    Publishing date 2019-06-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2020896-0
    ISSN 1469-3178 ; 1469-221X
    ISSN (online) 1469-3178
    ISSN 1469-221X
    DOI 10.15252/embr.201947995
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    Zs.A 5433: Show issues Location:
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  9. Article ; Online: Targeting apoptosis pathways in infections.

    Naderer, Thomas / Fulcher, Maria Cecilia

    Journal of leukocyte biology

    2018  Volume 103, Issue 2, Page(s) 275–285

    Abstract: The programmed cell death pathway of apoptosis is essential for mammalian development and immunity as it eliminates unwanted and dangerous cells. As part of the cellular immune response, apoptosis removes the replicative niche of intracellular pathogens ... ...

    Abstract The programmed cell death pathway of apoptosis is essential for mammalian development and immunity as it eliminates unwanted and dangerous cells. As part of the cellular immune response, apoptosis removes the replicative niche of intracellular pathogens and enables the resolution of infections. To subvert apoptosis, pathogens have evolved a diverse range of mechanisms. In some circumstances, however, pathogens express effector molecules that induce apoptotic cell death. In this review, we focus on selected host-pathogen interactions that affect apoptotic pathways. We discuss how pathogens control the fate of host cells and how this determines the outcome of infections. Finally, small molecule inhibitors that activate apoptosis in cancer cells can also induce apoptotic cell death of infected cells. This suggests that targeting host death factors to kill infected cells is a potential therapeutic option to treat infectious diseases.
    MeSH term(s) Animals ; Apoptosis/drug effects ; Apoptosis Regulatory Proteins/metabolism ; Communicable Diseases/drug therapy ; Disease Models, Animal ; Host-Pathogen Interactions ; Humans ; Immune System/physiology ; Mice, Knockout ; Mitochondria/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism
    Chemical Substances Apoptosis Regulatory Proteins ; Proto-Oncogene Proteins c-bcl-2
    Language English
    Publishing date 2018-01-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 605722-6
    ISSN 1938-3673 ; 0741-5400
    ISSN (online) 1938-3673
    ISSN 0741-5400
    DOI 10.1189/JLB.4MR0717-286R
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    Zs.A 603: Show issues Location:
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  10. Article ; Online: ChatGPT, Artificial Intelligence, and Suicide Prevention.

    Arendt, Florian / Till, Benedikt / Voracek, Martin / Kirchner, Stefanie / Sonneck, Gernot / Naderer, Brigitte / Pürcher, Paul / Niederkrotenthaler, Thomas

    Crisis

    2023  Volume 44, Issue 5, Page(s) 367–370

    MeSH term(s) Humans ; Artificial Intelligence ; Suicide Prevention
    Language English
    Publishing date 2023-09-22
    Publishing country Canada
    Document type Editorial
    ZDB-ID 801380-9
    ISSN 2151-2396 ; 0227-5910
    ISSN (online) 2151-2396
    ISSN 0227-5910
    DOI 10.1027/0227-5910/a000915
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