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  1. Book ; Online: Imaging Sensors and Applications

    Lee, Changho / Yoon, Changhan / Yoon, Changhan

    2021  

    Keywords Technology: general issues ; fluorescence LiDAR ; laser-induced fluorescence ; vegetation monitoring ; classification discrimination ; 3D measurement ; fringe projection ; 3D Fourier transform ; phase unwrapping ; phase measurement ; ultrasound ; photoacoustic imaging ; photoacoustic microscopy ; biomedical imaging ; multifocal point transducer ; wave patterns ; analytical model ; directivity pattern ; guided wave (GW) ; non-destructive testing (NDT) ; macro-fiber composite (MFC) ; transducer ; elastography ; soft tissue ; nonlinearity ; viscoelasticity ; acoustic emission ; hydrogel ; nanosilica ; transcranial ; skull bone ; aberration ; photoacoustic ; distortion ; brain imaging ; balloon catheter ; image guiding ; medical diagnostic imaging ; ultrasonic imaging ; abdominal ultrasound ; plane wave imaging ; diverging wave imaging ; synthetic focusing ; back muscle stiffness ; spine ; elasticity ; shear-wave elastography (SWE) ; tissue ultrasound palpation system (TUPS) ; reliability ; Young's modulus ; carfilzomib ; peripheral vasculature ; quantitative analysis ; transrectal probe ; optical lens ; ultrasound imaging ; prostate cancer ; optical coherence tomography ; quad-scanner scanning strategy ; whole-directional scanning ; full-directional imaging ; IoT ; remote control ; remote operation ; remote sharing economy ; research equipment sharing ; two-photon laser scanning microscopy ; MQTT ; deep learning ; ensemble learning ; brain tumor classification ; machine learning ; transfer learning ; around view monitoring system ; automatic camera calibration ; vision-based advanced driver assistance systems ; high-frequency ultrasound ; ophthalmic imaging ; synthetic aperture ; convex array transducer ; bandwidth expander ; ultrasound transducer device ; power amplifier ; super-resolution ; clinical applications ; n/a
    Size 1 electronic resource (350 pages)
    Publisher MDPI - Multidisciplinary Digital Publishing Institute
    Publishing place Basel, Switzerland
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021291617
    ISBN 9783036526058 ; 3036526056
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article: Optimization of Transposon Mutagenesis Methods in

    Kim, Sangha / Lee, Changhan

    Microorganisms

    2023  Volume 11, Issue 1

    Abstract: ... ...

    Abstract Pseudomonas
    Language English
    Publishing date 2023-01-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11010118
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica

    Sangha Kim / Changhan Lee

    Microorganisms, Vol 11, Iss 118, p

    2023  Volume 118

    Abstract: Pseudomonas is a widespread genus in various host and environmental niches. Pseudomonas exists even in extremely cold environments such as Antarctica. Pseudomonas antarctica is a psychrophilic bacterium isolated from Antarctica. P. antarctica is also ... ...

    Abstract Pseudomonas is a widespread genus in various host and environmental niches. Pseudomonas exists even in extremely cold environments such as Antarctica. Pseudomonas antarctica is a psychrophilic bacterium isolated from Antarctica. P. antarctica is also known to produce antimicrobial substances. Although P. antarctica can provide insight into how bacteria have adapted to low temperatures and has significant potential for developing novel antimicrobial substances, progress in genetic and molecular studies has not been achieved. Transposon mutagenesis is a useful tool to screen genes of interest in bacteria. Therefore, we attempted for the first time in P. antarctica to generate transposon insertion mutants using the transfer of a conjugational plasmid encoding a transposon. To increase the yield of transposon insertion mutants, we optimized the methods, in terms of temperature for conjugation, the ratio of donor and recipient during conjugation, and the concentration of antibiotics. Here, we describe the optimized methods to successfully generate transposon insertion mutants in P. antarctica .
    Keywords Pseudomonas ; Pseudomonas antarctica ; transposon ; transposon insertion mutant library ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  4. Article ; Online: Exploring the impact of nucleic acids on protein stability in bacterial cell lysate.

    Ham, Soojeong / Lee, Changhan

    Biochimica et biophysica acta. General subjects

    2023  Volume 1867, Issue 10, Page(s) 130445

    Abstract: Addition of salt enhanced thermal stability of model substrate proteins by reducing electrostatic repulsion between protein molecules. - However, the opposite effect was observed with bacterial cell lysate, indicating that certain molecules within the ... ...

    Abstract - Addition of salt enhanced thermal stability of model substrate proteins by reducing electrostatic repulsion between protein molecules. - However, the opposite effect was observed with bacterial cell lysate, indicating that certain molecules within the lysate could enhance protein stability via electrostatic interactions. - Such molecules present in cell lysate were found to be nucleic acids known to have a potent anti-aggregation activity toward proteins involving electrostatic interactions. - Nucleic acids showed chaperone activity in physiological salt concentration within cells and in buffer or medium commonly used in experiments. - The chaperone activity of nucleic acids should be taken into account when performing various in vitro assays using cell lysate or samples containing nucleic acids.
    MeSH term(s) Nucleic Acids ; Protein Stability ; Protein Folding ; Static Electricity
    Chemical Substances Nucleic Acids
    Language English
    Publishing date 2023-08-15
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbagen.2023.130445
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.247: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Nuclear transcriptional regulation by mitochondrial-encoded MOTS-c.

    Lee, Changhan

    Molecular & cellular oncology

    2019  Volume 6, Issue 2, Page(s) 1549464

    Abstract: Cellular stress response is coordinated through the communication between mitochondria and the nucleus. However, whereas mitochondria are regulated by nuclear-encoded proteins, the nucleus was considered ungoverned by mitochondrial-encoded factors. We ... ...

    Abstract Cellular stress response is coordinated through the communication between mitochondria and the nucleus. However, whereas mitochondria are regulated by nuclear-encoded proteins, the nucleus was considered ungoverned by mitochondrial-encoded factors. We recently reported that a mitochondrial-encoded peptide directly regulates the nuclear genome upon cellular stress, indicating an integrated bi-genomic cross-communication mechanism.
    Language English
    Publishing date 2019-02-15
    Publishing country United States
    Document type Journal Article
    ISSN 2372-3556
    ISSN 2372-3556
    DOI 10.1080/23723556.2018.1549464
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Recent Advances in Imaging Sensors and Applications.

    Yoon, Changhan / Lee, Changho

    Sensors (Basel, Switzerland)

    2021  Volume 21, Issue 12

    Abstract: Recent advances in sensor technology have allowed us to develop many interesting applications and enhance the quality of human life [ ... ]. ...

    Abstract Recent advances in sensor technology have allowed us to develop many interesting applications and enhance the quality of human life [...].
    Language English
    Publishing date 2021-06-09
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s21123970
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Mitochondrial-Encoded Peptide MOTS-c, Diabetes, and Aging-Related Diseases.

    Kong, Byung Soo / Lee, Changhan / Cho, Young Min

    Diabetes & metabolism journal

    2023  Volume 47, Issue 3, Page(s) 315–324

    Abstract: Mitochondria are complex metabolic organelles with manifold pathophysiological implications in diabetes. Currently published mitochondrial-encoded peptides, which are expressed from the mitochondrial open reading frame of the 12S ribosomal RNA type-c ( ... ...

    Abstract Mitochondria are complex metabolic organelles with manifold pathophysiological implications in diabetes. Currently published mitochondrial-encoded peptides, which are expressed from the mitochondrial open reading frame of the 12S ribosomal RNA type-c (MOTS-c), 16S rRNA (humanin and short humanin like peptide 1-6 [SHLP1-6]), or small human mitochondrial open reading frame over serine tRNA (SHMOOSE) are associated with regulation of cellular metabolism and insulin action in age-related diseases, such as type 2 diabetes mellitus. This review focuses mainly on recent advances in MOTS-c research with regards to diabetes, including both type 1 and type 2. The emerging understanding of MOTS-c in diabetes may provide insight into the development of new therapies for diabetes and other age or senescence-related diseases.
    MeSH term(s) Humans ; RNA, Ribosomal, 16S/metabolism ; Diabetes Mellitus, Type 2/metabolism ; Mitochondria/metabolism ; Peptides ; Transcription Factors/metabolism ; Aging
    Chemical Substances RNA, Ribosomal, 16S ; Peptides ; Transcription Factors
    Language English
    Publishing date 2023-02-24
    Publishing country Korea (South)
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2602402-0
    ISSN 2233-6087 ; 2233-6087
    ISSN (online) 2233-6087
    ISSN 2233-6087
    DOI 10.4093/dmj.2022.0333
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Protocol for the assessment of human T cell activation by real-time metabolic flux analysis

    Byung Soo Kong / Changhan Lee / Young Min Cho

    STAR Protocols, Vol 3, Iss 1, Pp 101084- (2022)

    2022  

    Abstract: Summary: The elevation of glycolysis in autoreactive T cells is a key target for the prevention and treatment of T cell-related autoimmune diseases, such as type 1 diabetes (T1D). Here, we describe a simple and efficient protocol for isolating human ... ...

    Abstract Summary: The elevation of glycolysis in autoreactive T cells is a key target for the prevention and treatment of T cell-related autoimmune diseases, such as type 1 diabetes (T1D). Here, we describe a simple and efficient protocol for isolating human peripheral blood mononuclear cells (PBMCs) and T cells, and the subsequent assessment of T cell glycolysis using Seahorse analyzer. This protocol is useful to analyze different subsets of T cells and applicable to different autoimmune disease models (i.e., T1D, multiple sclerosis).For complete details on the use and execution of this profile, please refer to Kong et al. (2021).
    Keywords Classification Description: Cell Biology ; Cell isolation ; Cell-based Assays ; Immunology ; Metabolism ; Science (General) ; Q1-390
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Aging: All roads lead to mitochondria.

    Son, Jyung Mean / Lee, Changhan

    Seminars in cell & developmental biology

    2021  Volume 116, Page(s) 160–168

    Abstract: Mitochondria were described as early as 1890 as ubiquitous intracellular structures by Ernster and Schatz (1981) [1]. Since then, the accretion of knowledge in the past century has revealed much of the molecular details of mitochondria, ranging from ... ...

    Abstract Mitochondria were described as early as 1890 as ubiquitous intracellular structures by Ernster and Schatz (1981) [1]. Since then, the accretion of knowledge in the past century has revealed much of the molecular details of mitochondria, ranging from mitochondrial origin, structure, metabolism, genetics, and signaling, and their implications in health and disease. We now know that mitochondria are remarkably multifunctional and deeply intertwined with many vital cellular processes. They are quasi-self organelles that still possess remnants of its bacterial ancestry, including an independent genome. The mitochondrial free radical theory of aging (MFRTA), which postulated that aging is a product of oxidative damage to mitochondrial DNA, provided a conceptual framework that put mitochondria on the map of aging research. However, several studies have more recently challenged the general validity of the theory, favoring novel ideas based on emerging evidence to understand how mitochondria contribute to aging and age-related diseases. One prominent topic of investigation lies on the fact that mitochondria are not only production sites for bioenergetics and macromolecules, but also regulatory hubs that communicate and coordinate many vital physiological processes at the cellular and organismal level. The bi-directional communication and coordination between the co-evolved mitochondrial and nuclear genomes is especially interesting in terms of cellular regulation. Mitochondria are dynamic and adaptive, rendering their function sensitive to cellular context. Tissues with high energy demands, such as the brain, seem to be uniquely affected by age-dependent mitochondrial dysfunction, providing a foundation for the development of novel mitochondrial-based therapeutics and diagnostics.
    MeSH term(s) Aging/genetics ; Animals ; Humans ; Mice ; Mitochondria/metabolism ; Rats
    Language English
    Publishing date 2021-03-23
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1312473-0
    ISSN 1096-3634 ; 1084-9521
    ISSN (online) 1096-3634
    ISSN 1084-9521
    DOI 10.1016/j.semcdb.2021.02.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2918: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  10. Article ; Online: Mitochondrial-encoded MOTS-c prevents pancreatic islet destruction in autoimmune diabetes.

    Kong, Byung Soo / Min, Se Hee / Lee, Changhan / Cho, Young Min

    Cell reports

    2021  Volume 36, Issue 11, Page(s) 109724

    Language English
    Publishing date 2021-09-14
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2021.109724
    Database MEDical Literature Analysis and Retrieval System OnLINE

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