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  1. Article ; Online: Protocol for High Throughput Screening of Antibody Phage Libraries.

    Singh, Vanshika / Garg, Sonal / Raj, Nisha / Lukose, Asha / Jamwal, Deepti / Perween, Reshma / Dhyani, Samridhi / Parray, Hilal Ahamed / Sharma, Chandresh / Kumar, Rajesh

    Bio-protocol

    2022  Volume 12, Issue 12, Page(s) e4450

    Abstract: Phage display is a proven and widely used technology for selecting specific antibodies against desired targets. However, an immense amount of effort is required to identify and screen the desired positive clones from large and diverse combinatorial ... ...

    Abstract Phage display is a proven and widely used technology for selecting specific antibodies against desired targets. However, an immense amount of effort is required to identify and screen the desired positive clones from large and diverse combinatorial libraries. On the other hand, the selection of positive binding clones from synthetic and semi-synthetic libraries has an inherent bias toward clones with randomly produced amber stop codons, making it more difficult to identify desirable binding antibodies. To overcome the screening of desired clones with amber codons, we present a step-by-step approach for effective phage library screening to isolate useful antibodies. The procedure calls for creating a simple new vector system for soluble production of phage ELISA positive binding clones with one or more amber stop codons in their single-chain antibody fragment (scFv) gene sequences, which is otherwise difficult in standard screening. Graphical abstract.
    Language English
    Publishing date 2022-06-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4450
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A rapid novel strategy for screening of antibody phage libraries for production, purification, and functional characterization of amber stop codons containing single-chain antibody fragments.

    Perween, Reshma / Ahmed, Shubbir / Shrivastava, Tripti / Parray, Hilal A / Singh, Balwant / Pindari, Kamal S / Sharma, Chandresh / Shukla, Shivangi / Sinha, Subrata / Panchal, Anil Kumar / Kumar, Rajesh

    Biotechnology progress

    2021  Volume 37, Issue 3, Page(s) e3136

    Abstract: Phage display antibody (PDA) libraries, allows the rapid isolation and characterization of high specificity monoclonal antibodies for therapeutic and diagnostic applications. However, selection of positive binding clones from synthetic and semi-synthetic ...

    Abstract Phage display antibody (PDA) libraries, allows the rapid isolation and characterization of high specificity monoclonal antibodies for therapeutic and diagnostic applications. However, selection of positive binding clones from synthetic and semi-synthetic libraries has an inherent bias towards clones containing randomly generated amber stop codons, complicating the identification of high affinity binding antibodies. We screened Tomlinson I and J library against receptor binding domain (RBD) of SARS CoV2, eight clones which showed positive binding in phage ELISA, contained one or more amber stop codons in their single-chain antibody fragment (scFv) gene sequences. The presence of amber stop codons within the antibody sequence causes the premature termination of soluble form of scFv expression in nonsuppressor Escherichia coli strain. In the present study, we have used a novel strategy that allows soluble expression of scFvs having amber stop codon in their gene sequences (without phage PIII protein fusion), in the suppressor strain. This strategy of introduction of Ochre (TAA) codon at the junction of scFv and PIII gene, speeds up the initial screening process which is critical for selecting the right scFvs for further studies. Present strategy leads to the identification of a scFv, B8 that binds specifically with nanomolar affinity toward SARS CoV 2 RBD, which otherwise lost in terms of traditional methodology.
    Language English
    Publishing date 2021-03-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 165657-0
    ISSN 1520-6033 ; 8756-7938
    ISSN (online) 1520-6033
    ISSN 8756-7938
    DOI 10.1002/btpr.3136
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    Zs.A 4253: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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  3. Article ; Online: Inhalation monoclonal antibody therapy: a new way to treat and manage respiratory infections.

    Parray, Hilal Ahmad / Shukla, Shivangi / Perween, Reshma / Khatri, Ritika / Shrivastava, Tripti / Singh, Vanshika / Murugavelu, Praveenkumar / Ahmed, Shubbir / Samal, Sweety / Sharma, Chandresh / Sinha, Subrata / Luthra, Kalpana / Kumar, Rajesh

    Applied microbiology and biotechnology

    2021  Volume 105, Issue 16-17, Page(s) 6315–6332

    Abstract: The route of administration of a therapeutic agent has a substantial impact on its success. Therapeutic antibodies are usually administered systemically, either directly by intravenous route, or indirectly by intramuscular or subcutaneous injection. ... ...

    Abstract The route of administration of a therapeutic agent has a substantial impact on its success. Therapeutic antibodies are usually administered systemically, either directly by intravenous route, or indirectly by intramuscular or subcutaneous injection. However, treatment of diseases contained within a specific tissue necessitates a better alternate route of administration for targeting localised infections. Inhalation is a promising non-invasive strategy for antibody delivery to treat respiratory maladies because it provides higher concentrations of antibody in the respiratory airways overcoming the constraints of entry through systemic circulation and uncertainity in the amount reaching the target tissue. The nasal drug delivery route is one of the extensively researched modes of administration, and nasal sprays for molecular drugs are deemed successful and are presently commercially marketed. This review highlights the current state and future prospects of inhaled therapies, with an emphasis on the use of monoclonal antibodies for the treatment of respiratory infections, as well as an overview of their importance, practical challenges, and clinical trial outcomes.Key points• Immunologic strategies for preventing mucosal transmission of respiratory pathogens.• Mucosal-mediated immunoprophylaxis could play a major role in COVID-19 prevention.• Applications of monoclonal antibodies in passive immunisation.
    MeSH term(s) Antibodies, Monoclonal/therapeutic use ; COVID-19 ; Humans ; Immunization, Passive ; Immunotherapy ; SARS-CoV-2
    Chemical Substances Antibodies, Monoclonal
    Language English
    Publishing date 2021-08-23
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-021-11488-4
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    Zs.A 2229: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article: Inhalation monoclonal antibody therapy: a new way to treat and manage respiratory infections

    Parray, Hilal Ahmad / Shukla, Shivangi / Perween, Reshma / Khatri, Ritika / Shrivastava, Tripti / Singh, Vanshika / Murugavelu, Praveenkumar / Ahmed, Shubbir / Samal, Sweety / Sharma, Chandresh / Sinha, Subrata / Luthra, Kalpana / Kumar, Rajesh

    Applied microbiology and biotechnology. 2021 Aug., v. 105, no. 16-17

    2021  

    Abstract: The route of administration of a therapeutic agent has a substantial impact on its success. Therapeutic antibodies are usually administered systemically, either directly by intravenous route, or indirectly by intramuscular or subcutaneous injection. ... ...

    Abstract The route of administration of a therapeutic agent has a substantial impact on its success. Therapeutic antibodies are usually administered systemically, either directly by intravenous route, or indirectly by intramuscular or subcutaneous injection. However, treatment of diseases contained within a specific tissue necessitates a better alternate route of administration for targeting localised infections. Inhalation is a promising non-invasive strategy for antibody delivery to treat respiratory maladies because it provides higher concentrations of antibody in the respiratory airways overcoming the constraints of entry through systemic circulation and uncertainity in the amount reaching the target tissue. The nasal drug delivery route is one of the extensively researched modes of administration, and nasal sprays for molecular drugs are deemed successful and are presently commercially marketed. This review highlights the current state and future prospects of inhaled therapies, with an emphasis on the use of monoclonal antibodies for the treatment of respiratory infections, as well as an overview of their importance, practical challenges, and clinical trial outcomes.Key points• Immunologic strategies for preventing mucosal transmission of respiratory pathogens.• Mucosal-mediated immunoprophylaxis could play a major role in COVID-19 prevention.• Applications of monoclonal antibodies in passive immunisation.
    Keywords COVID-19 infection ; biotechnology ; breathing ; clinical trials ; immunization ; intravenous injection ; microbiology ; monoclonal antibodies ; nose ; subcutaneous injection
    Language English
    Dates of publication 2021-08
    Size p. 6315-6332.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note Review
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-021-11488-4
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 79/727: Show issues

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  5. Article ; Online: The SARS CoV-2 spike directed non-neutralizing polyclonal antibodies cross-react with Human immunodeficiency virus (HIV-1) gp41.

    Perween, Reshma / PraveenKumar, Murugavelu / Shrivastava, Tripti / Parray, Hilal Ahmed / Singh, Vanshika / Singh, Swarandeep / Chiranjivi, Adarsh / Jakhar, Kamini / Sonar, Sudipta / Tiwari, Mahima / Reema / Panchal, Anil Kumar / Sharma, Chandresh / Rathore, Deepak Kumar / Ahamed, Shubbir / Samal, Sweety / Mani, Shailendra / Bhattacharyya, Sankar / Das, Supratik /
    Luthra, Kalpana / Kumar, Rajesh

    International immunopharmacology

    2021  Volume 101, Issue Pt B, Page(s) 108187

    Abstract: Cross-reactivity among the two diverse viruses is believed to originate from the concept of antibodies recognizing similar epitopes on the two viral surfaces. Cross-reactive antibody responses have been seen in previous variants of SARS and SARS-CoV-2, ... ...

    Abstract Cross-reactivity among the two diverse viruses is believed to originate from the concept of antibodies recognizing similar epitopes on the two viral surfaces. Cross-reactive antibody responses have been seen in previous variants of SARS and SARS-CoV-2, but little is known about the cross reactivity with other similar RNA viruses like HIV-1. In the present study, we examined the reactivity the SARS-CoV-2 directed antibodies, via spike, immunized mice sera and demonstrated whether they conferred any cross-reactive neutralization against HIV-1. Our findings show that SARS-CoV-2 spike immunized mice antibodies cross-react with the HIV-1 Env protein. Cross-neutralization among the two viruses is uncommon, suggesting the presence of a non-neutralizing antibody response to conserved epitopes amongst the two viruses. Our results indicate, that SARS-CoV-2 spike antibody cross reactivity is targeted towards the gp41 region of the HIV-1 Env (gp160) protein. Overall, our investigation not only answers a crucial question about the understanding of cross-reactive epitopes of antibodies generated in different viral infections, but also provides critical evidence for developing vaccine immunogens and novel treatment strategies with enhanced efficacy capable of recognising diverse pathogens with similar antigenic features.
    MeSH term(s) Animals ; Antibodies, Viral/immunology ; Cross Reactions ; HIV Envelope Protein gp41/immunology ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Recombinant Proteins/immunology ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/immunology ; Mice
    Chemical Substances Antibodies, Viral ; HIV Envelope Protein gp41 ; Recombinant Proteins ; Spike Glycoprotein, Coronavirus ; gp41 protein, Human immunodeficiency virus 1 ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2021-09-28
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2043785-7
    ISSN 1878-1705 ; 1567-5769
    ISSN (online) 1878-1705
    ISSN 1567-5769
    DOI 10.1016/j.intimp.2021.108187
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5408: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Non-neutralizing SARS CoV-2 directed polyclonal antibodies demonstrate cross-reactivity with the HA glycans of influenza virus.

    Murugavelu, Praveenkumar / Perween, Reshma / Shrivastava, Tripti / Singh, Vanshika / Ahmad Parray, Hilal / Singh, Swarandeep / Chiranjivi, Adarsh Kumar / Thiruvengadam, Ramachandran / Singh, Savita / Yadav, Naveen / Jakhar, Kamini / Sonar, Sudipta / Mani, Shailendra / Bhattacharyya, Sankar / Sharma, Chandresh / Vishwakarma, Preeti / Khatri, Ritika / Kumar Panchal, Anil / Das, Supratik /
    Ahmed, Shubbir / Samal, Sweety / Kshetrapal, Pallavi / Bhatnagar, Shinjini / Luthra, Kalpana / Kumar, Rajesh

    International immunopharmacology

    2021  Volume 99, Page(s) 108020

    Abstract: The spike protein of the SARS-CoV-2 virus is the foremost target for the designing of vaccines and therapeutic antibodies and also acts as a crucial antigen in the assessment of COVID-19 immune responses. The enveloped viruses; such as SARS-CoV-2, Human ... ...

    Abstract The spike protein of the SARS-CoV-2 virus is the foremost target for the designing of vaccines and therapeutic antibodies and also acts as a crucial antigen in the assessment of COVID-19 immune responses. The enveloped viruses; such as SARS-CoV-2, Human Immunodeficiency Virus-1 (HIV-1) and influenza, often hijack host-cell glycosylation pathways and influence pathobiology and immune selection. These glycan motifs can lead to either immune evasion or viral neutralization by the production of cross-reactive antibodies that can lead to antibody-dependent enhancement (ADE) of infection. Potential cross-protection from influenza vaccine has also been reported in COVID-19 infected individuals in several epidemiological studies recently; however, the scientific basis for these observations remains elusive. Herein, we show that the anti-SARS-CoV2 antibodies cross-reacts with the Hemagglutinin (HA) protein. This phenomenon is common to both the sera from convalescent SARS-CoV-2 donors and spike immunized mice, although these antibodies were unable to cross-neutralize, suggesting the presence of a non-neutralizing antibody response. Epitope mapping suggests that the cross-reactive antibodies are targeted towards glycan epitopes of the SARS-CoV-2 spike and HA. Overall, our findings address the cross-reactive responses, although non-neutralizing, elicited against RNA viruses and warrant further studies to investigate whether such non-neutralizing antibody responses can contribute to effector functions such as antibody-dependent cellular cytotoxicity (ADCC) or ADE.
    MeSH term(s) Animals ; Antibodies, Neutralizing ; Antigen-Antibody Reactions ; Binding Sites, Antibody/immunology ; COVID-19/immunology ; Cell Culture Techniques ; Chlorocebus aethiops ; Cross Reactions/immunology ; Dogs ; Epitope Mapping ; Epitopes/immunology ; Glycosylation ; Hemagglutinin Glycoproteins, Influenza Virus/immunology ; Humans ; Influenza Vaccines/immunology ; Madin Darby Canine Kidney Cells ; Membrane Glycoproteins/metabolism ; Mice ; Mice, Inbred C57BL ; SARS-CoV-2/immunology ; Spike Glycoprotein, Coronavirus/immunology ; Vero Cells
    Chemical Substances Antibodies, Neutralizing ; Epitopes ; Hemagglutinin Glycoproteins, Influenza Virus ; Influenza Vaccines ; Membrane Glycoproteins ; Spike Glycoprotein, Coronavirus
    Language English
    Publishing date 2021-07-29
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2043785-7
    ISSN 1878-1705 ; 1567-5769
    ISSN (online) 1878-1705
    ISSN 1567-5769
    DOI 10.1016/j.intimp.2021.108020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5408: Show issues Location:
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    ab Jg. 2022: Lesesaal (EG)

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