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  1. Book ; Online: Towards a Metropolitan City Centre for Brussels

    Berger, Mathieu / Boussauw, Kobe / Corijn, Eric / Creten, Alexis / da Schio, Nicola / Decroly, Jean-Michel / De Visscher, Jean-Philippe / Donnen, Alexandre / Fenton, Gabrielle / Genard, Jean-Louis / Hardy, Margaux / Houlstan-Hasaerts, Rafaella / HUBERT, Michel / Jaumain, Serge / Keserü, Imre / Laki, Giulietta / Ledent, Gérald / Letesson, Quentin / Mezoued, Aniss M. /
    Neuwels, Julie / Perilleux, Hugo / Rosa, Elisabetta / Salembier, Chloé / Strale, Mathieu / Vaesen, Joost

    2020  

    Keywords Regional & area planning ; pedestrianization ; city centre ; sustainability ; urban development
    Size 1 electronic resource (342 pages)
    Publisher Éditions de l'Université de Bruxelles
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021031171
    ISBN 9789057185175 ; 9057185172
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Cryo-EM reveals binding of linoleic acid to SARS-CoV-2 spike glycoprotein, suggesting an antiviral treatment strategy.

    Toelzer, Christine / Gupta, Kapil / Berger, Imre / Schaffitzel, Christiane

    Acta crystallographica. Section D, Structural biology

    2023  Volume 79, Issue Pt 2, Page(s) 111–121

    Abstract: The COVID-19 pandemic and concomitant lockdowns presented a global health challenge and triggered unprecedented research efforts to elucidate the molecular mechanisms and pathogenicity of SARS-CoV-2. The spike glycoprotein decorating the surface of SARS- ... ...

    Abstract The COVID-19 pandemic and concomitant lockdowns presented a global health challenge and triggered unprecedented research efforts to elucidate the molecular mechanisms and pathogenicity of SARS-CoV-2. The spike glycoprotein decorating the surface of SARS-CoV-2 virions is a prime target for vaccine development, antibody therapy and serology as it binds the host cell receptor and is central for viral cell entry. The electron cryo-microscopy structure of the spike protein revealed a hydrophobic pocket in the receptor-binding domain that is occupied by an essential fatty acid, linoleic acid (LA). The LA-bound spike protein adopts a non-infectious locked conformation which is more stable than the infectious form and shields important immunogenic epitopes. Here, the impact of LA binding on viral infectivity and replication, and the evolutionary conservation of the pocket in other highly pathogenic coronaviruses, including SARS-CoV-2 variants of concern (VOCs), are reviewed. The importance of LA metabolic products, the eicosanoids, in regulating the human immune response and inflammation is highlighted. Lipid and fatty-acid binding to a hydrophobic pocket in proteins on the virion surface appears to be a broader strategy employed by viruses, including picornaviruses and Zika virus. Ligand binding stabilizes their protein structure and assembly, and downregulates infectivity. In the case of rhinoviruses, this has been exploited to develop small-molecule antiviral drugs that bind to the hydrophobic pocket. The results suggest a COVID-19 antiviral treatment based on the LA-binding pocket.
    MeSH term(s) Humans ; Antiviral Agents/metabolism ; Antiviral Agents/pharmacology ; Communicable Disease Control ; COVID-19/therapy ; COVID-19/virology ; Cryoelectron Microscopy ; Linoleic Acid/metabolism ; Linoleic Acid/pharmacology ; Pandemics ; SARS-CoV-2/drug effects ; SARS-CoV-2/metabolism ; SARS-CoV-2/ultrastructure ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/ultrastructure
    Chemical Substances Antiviral Agents ; Linoleic Acid (9KJL21T0QJ) ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2023-01-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2968623-4
    ISSN 2059-7983 ; 0907-4449
    ISSN (online) 2059-7983
    ISSN 0907-4449
    DOI 10.1107/S2059798323000049
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The SARS-CoV-2 spike protein: balancing stability and infectivity.

    Berger, Imre / Schaffitzel, Christiane

    Cell research

    2020  Volume 30, Issue 12, Page(s) 1059–1060

    MeSH term(s) Angiotensin-Converting Enzyme 2/metabolism ; COVID-19/pathology ; COVID-19/virology ; Humans ; Mutation ; Protein Binding ; Protein Domains ; Protein Stability ; Protein Structure, Tertiary ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/metabolism ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/metabolism
    Chemical Substances Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23)
    Keywords covid19
    Language English
    Publishing date 2020-10-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 1319303-x
    ISSN 1748-7838 ; 1001-0602
    ISSN (online) 1748-7838
    ISSN 1001-0602
    DOI 10.1038/s41422-020-00430-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5283: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  5. Article ; Online: The MultiBac BEVS: Basics, applications, performance and recent developments.

    Gorda, Barbara / Toelzer, Christine / Aulicino, Francesco / Berger, Imre

    Methods in enzymology

    2021  Volume 660, Page(s) 129–154

    Abstract: The baculovirus expression vector system (BEVS) delivers high yield heterologous protein expression and is widely used in academic and industrial R&D. The proteins produced enable many applications including structure/function analysis, drug screening ... ...

    Abstract The baculovirus expression vector system (BEVS) delivers high yield heterologous protein expression and is widely used in academic and industrial R&D. The proteins produced enable many applications including structure/function analysis, drug screening and manufacture of protein therapeutics. Vital cellular functions are controlled by multi-protein complexes, MultiBac, a BEVS specifically designed for heterologous multigene delivery and expression, has unlocked many of these machines to atomic resolution studies. Baculovirus can accommodate very large foreign DNA cargo for faithful delivery into a target host cell, tissue or organism. Engineered MultiBac variants exploit this valuable feature for delivery of customized multifunctional DNA circuitry in mammalian cells and for production of virus-like particles for vaccines manufacture. Here, latest developments and applications of the MultiBac system are reviewed.
    MeSH term(s) Animals ; Baculoviridae/genetics ; Baculoviridae/metabolism ; DNA/genetics ; DNA/metabolism ; Genetic Vectors/genetics ; Mammals/genetics ; Proteins/metabolism ; Recombinant Proteins/metabolism
    Chemical Substances Proteins ; Recombinant Proteins ; DNA (9007-49-2)
    Language English
    Publishing date 2021-07-13
    Publishing country United States
    Document type Journal Article
    ISSN 1557-7988
    ISSN (online) 1557-7988
    DOI 10.1016/bs.mie.2021.06.018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Bottom-up assembly of viral replication cycles.

    Staufer, Oskar / Gantner, Gösta / Platzman, Ilia / Tanner, Klaus / Berger, Imre / Spatz, Joachim P

    Nature communications

    2022  Volume 13, Issue 1, Page(s) 6530

    Abstract: Bottom-up synthetic biology provides new means to understand living matter by constructing minimal life-like systems. This principle can also be applied to study infectious diseases. Here we summarize approaches and ethical considerations for the bottom- ... ...

    Abstract Bottom-up synthetic biology provides new means to understand living matter by constructing minimal life-like systems. This principle can also be applied to study infectious diseases. Here we summarize approaches and ethical considerations for the bottom-up assembly of viral replication cycles.
    MeSH term(s) Synthetic Biology ; Virus Replication
    Language English
    Publishing date 2022-11-02
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-022-33661-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: The MultiBac system: a perspective.

    Berger, Imre / Tölzer, Christine / Gupta, Kapil

    Emerging topics in life sciences

    2021  Volume 3, Issue 5, Page(s) 477–482

    Abstract: Baculovirus expression is a time-tested technique to produce proteins in insect cells, in high quality and quantity for a range of applications. MultiBac is a baculovirus expression system we developed originally for producing multiprotein complexes ... ...

    Abstract Baculovirus expression is a time-tested technique to produce proteins in insect cells, in high quality and quantity for a range of applications. MultiBac is a baculovirus expression system we developed originally for producing multiprotein complexes comprising many subunits, for structural and mechanistic studies. First introduced in 2004, MultiBac is now in use in many laboratories worldwide, accelerating research programmes in academia and industry. We have continuously optimized our MultiBac system, providing customized reagents and standard operating protocols to facilitate its use also by non-specialists. More recently, we have generated MultiBac genomes tailored for specific purposes, for example, to produce humanized glycoproteins, high-value pharmaceutical targets including kinases, viral polymerases, and virus-like particles (VLPs) as promising vaccine candidates. By altering the host tropism of the baculovirion, we created MultiBacMam, a heterologous DNA delivery toolkit to target mammalian cells, tissues and organisms. Introducing CRISPR/Cas modalities, we set the stage for large-scale genomic engineering applications utilizing this high-capacity DNA delivery tool. Exploiting synthetic biology approaches and bottom-up design, we engage in optimizing the properties of our baculoviral genome, also to improve manufacturing at scale. Here we provide a perspective of our MultiBac system and its developments, past, present and future.
    Language English
    Publishing date 2021-02-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2882721-1
    ISSN 2397-8554 ; 2397-8554 ; 2397-8562
    ISSN (online) 2397-8554
    ISSN 2397-8554 ; 2397-8562
    DOI 10.1042/ETLS20190084
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Bottom-up assembly of viral replication cycles

    Oskar Staufer / Gösta Gantner / Ilia Platzman / Klaus Tanner / Imre Berger / Joachim P. Spatz

    Nature Communications, Vol 13, Iss 1, Pp 1-

    2022  Volume 10

    Abstract: Synthetic biology encompasses engineering approaches to either redesign biological organisms with new molecular abilities or build up organism from the bottom to improve our understanding of life. Here, the authors summarize current approaches that can ... ...

    Abstract Synthetic biology encompasses engineering approaches to either redesign biological organisms with new molecular abilities or build up organism from the bottom to improve our understanding of life. Here, the authors summarize current approaches that can be applied for the synthetic engineering of viral replication cycles and discuss the potential DURC risk associated with such bottom-up approaches in infectious disease.
    Keywords Science ; Q
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Engineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders.

    Buzas, Dora / Sun, Huan / Toelzer, Christine / Yadav, Sathish K N / Borucu, Ufuk / Gautam, Gunjan / Gupta, Kapil / Bufton, Joshua C / Capin, Julien / Sessions, Richard B / Garzoni, Frederic / Berger, Imre / Schaffitzel, Christiane

    Structure (London, England : 1993)

    2024  Volume 32, Issue 3, Page(s) 342–351.e6

    Abstract: Adenovirus-derived nanoparticles (ADDomer) comprise 60 copies of adenovirus penton base protein (PBP). ADDomer is thermostable, rendering the storage, transport, and deployment of ADDomer-based therapeutics independent of a cold chain. To expand the ... ...

    Abstract Adenovirus-derived nanoparticles (ADDomer) comprise 60 copies of adenovirus penton base protein (PBP). ADDomer is thermostable, rendering the storage, transport, and deployment of ADDomer-based therapeutics independent of a cold chain. To expand the scope of ADDomers for new applications, we engineered ADDobodies, representing PBP crown domain, genetically separated from PBP multimerization domain. We inserted heterologous sequences into hyper-variable loops, resulting in monomeric, thermostable ADDobodies expressed at high yields in Escherichia coli. The X-ray structure of an ADDobody prototype validated our design. ADDobodies can be used in ribosome display experiments to select a specific binder against a target, with an enrichment factor of ∼10
    MeSH term(s) Binding Sites ; Protein Engineering
    Language English
    Publishing date 2024-01-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1213087-4
    ISSN 1878-4186 ; 0969-2126
    ISSN (online) 1878-4186
    ISSN 0969-2126
    DOI 10.1016/j.str.2023.12.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4141: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  10. Article: Synthetic Virus-Derived Nanosystems (SVNs) for Delivery and Precision Docking of Large Multifunctional DNA Circuitry in Mammalian Cells.

    Aulicino, Francesco / Capin, Julien / Berger, Imre

    Pharmaceutics

    2020  Volume 12, Issue 8

    Abstract: DNA delivery is at the forefront of current research efforts in gene therapy and synthetic biology. Viral vectors have traditionally dominated the field; however, nonviral delivery systems are increasingly gaining traction. Baculoviruses are arthropod- ... ...

    Abstract DNA delivery is at the forefront of current research efforts in gene therapy and synthetic biology. Viral vectors have traditionally dominated the field; however, nonviral delivery systems are increasingly gaining traction. Baculoviruses are arthropod-specific viruses that can be easily engineered and repurposed to accommodate and deliver large sequences of exogenous DNA into mammalian cells, tissues, or ultimately organisms. These synthetic virus-derived nanosystems (SVNs) are safe, readily customized, and can be manufactured at scale. By implementing clustered regularly interspaced palindromic repeats (CRISPR) associated protein (CRISPR/Cas) modalities into this system, we developed SVNs capable of inserting complex DNAs into genomes, at base pair precision. We anticipate a major role for SVNs as an attractive alternative to viral vectors in accelerating genome engineering and gene therapy applications in the future.
    Language English
    Publishing date 2020-08-11
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics12080759
    Database MEDical Literature Analysis and Retrieval System OnLINE

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