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Article ; Online: Daming capsule, a hypolipidaemic drug, lowers blood lipids by activating the AMPK signalling pathway.

Bian, Yu / Li, Xin / Ju, Jiaming / Liang, Hangfei / Hu, Xueling / Dong, Lei / Wang, Ning / Li, Jiamin / Zhang, Yan / Yang, Baofeng

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2019 Volume 117, Page(s) 109176

Abstract: ... diseases. Daming capsule (DMC), a medicine for lowering blood lipids, is marketed in China ...

Abstract	Background/aims: Hyperlipidaemia is a major risk factor for cardiovascular and cerebrovascular diseases. Daming capsule (DMC), a medicine for lowering blood lipids, is marketed in China; however, its mechanism is unclear. The present study aimed to investigate the mechanism by which DMC reduces blood lipids. Methods and results: A rat model of hyperlipidaemia was established by feeding rats a high-fat diet (HFD), and the serum lipid levels were detected with an automatic biochemical analyser. DMC (162 mg/kg) and atorvastatin calcium (10 mg/kg) were orally administered to the hyperlipidaemic rats for 4 weeks. HFD feeding markedly induced increases in the levels of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-c); however, DMC treatment significantly decreased the levels of TC, TG, and LDL-c in rats serum. Meanwhile, the hepatic TC and TG levels, liver weight/body weight ratio, and body weight were significantly lower in the DMC-treated rats than in the HFD rats. Moreover, DMC significantly alleviated hepatomegaly, hepatic lipid deposition, and hepatic steatosis. The protein expression level of phospho-adenosine monophosphate-activated protein kinase (p-AMPK) (Thr172) in HFD rat livers was lower than that in normal rat livers, whereas it increased in the liver of the DMC-treated rats; however, the protein expression level of total-AMPK in the liver was not different among the groups. The AMPK-activating effect of DMC was blocked by Compound C (a specific AMPK inhibitor) in HepG2 cells. Additionally, DMC considerably increased peroxisome proliferator-activated receptor-alpha (PPARα) protein expression and lipoprotein lipase (LPL) transcription and concentration in the liver. This effect of DMC was also inhibited by Compound C in HepG2 cells. DMC also promoted LDL receptor (LDLR) protein expression by activating AMPK. We further found that DMC reduced the levels of TC and TG in oleic acid-treated HepG2 cells, and it restored the expression levels of p-AMPK, PPARα, LPL, and LDLR compared to the decreased levels observed in oleic acid-treated HepG2 cells. Conclusion: DMC lowered lipids in serum and the liver by activating AMPK. On the one hand, the activation of AMPK enhanced PPARα expression and LPL transcription to lead to the hydrolysis of TG; on the other hand, it increased LDLR protein expression to promote lipid metabolism.
MeSH term(s)	AMP-Activated Protein Kinases/metabolism ; Animals ; Cell Line, Tumor ; China ; Diet, High-Fat/adverse effects ; Fatty Liver/metabolism ; Hep G2 Cells ; Humans ; Hyperlipidemias/drug therapy ; Hyperlipidemias/metabolism ; Hypolipidemic Agents/pharmacology ; Lipid Metabolism/drug effects ; Lipids/blood ; Lipoprotein Lipase/metabolism ; Liver/drug effects ; Liver/metabolism ; Male ; Oleic Acid/metabolism ; PPAR alpha/metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, LDL/metabolism ; Signal Transduction/drug effects ; Triglycerides/blood
Chemical Substances	Hypolipidemic Agents ; Lipids ; PPAR alpha ; Receptors, LDL ; Triglycerides ; Oleic Acid (2UMI9U37CP) ; AMP-Activated Protein Kinases (EC 2.7.11.31) ; Lipoprotein Lipase (EC 3.1.1.34)
Language	English
Publishing date	2019-07-01
Publishing country	France
Document type	Journal Article
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2019.109176
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Article ; Online: A new species of Music frog (Anura, Ranidae, Nidirana) from Mt Daming, Guangxi, China

Zhi-Tong Lyu / Zhong Huang / Xiao-Wen Liao / Li Lin / Yong Huang / Ying-Yong Wang / Yun-Ming Mo

ZooKeys, Vol 1059, Iss , Pp 35-

2021 Volume 56

Abstract: ... of specimens collected from Mt Daming, Guangxi, southern China. The new species is close to N. yeae, N. daunchina, N ...

Abstract	Nidirana guangxiensis sp. nov., a new music frog species, is proposed, based on a series of specimens collected from Mt Daming, Guangxi, southern China. The new species is close to N. yeae, N. daunchina, N. yaoica, and N. chapaensis from southwestern and south-central China and northern Indochina, while the relationships among these species remain unresolved. Nidirana guangxiensis sp. nov. can be distinguished from all known congeners by the genetic divergences in the mitochondrial 16S and COI genes, the behavior of nest construction, the advertisement call containing 6–11 rapidly repeated regular notes, and a combination of morphological characteristics. Furthermore, the Nidirana populations recorded in Guangxi are clarified in this work, providing valuable new information on the knowledge of the genus Nidirana.
Keywords	Zoology ; QL1-991
Language	English
Publishing date	2021-09-01T00:00:00Z
Publisher	Pensoft Publishers
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: A new species of Music frog (Anura, Ranidae, Nidirana) from Mt Daming, Guangxi, China

Lyu, Zhi-Tong / Huang*, Zhong / Liao, Xiao-Wen / Lin, Li / Huang, Yong / Wang, Ying-Yong / Mo, Yun-Ming

ZooKeys. 2021 Sept. 01, v. 1059

2021

Abstract: ... of specimens collected from Mt Daming, Guangxi, southern China. The new species is close to N. yeae, N. daunchina, N ...

Abstract	Nidirana guangxiensis sp. nov., a new music frog species, is proposed, based on a series of specimens collected from Mt Daming, Guangxi, southern China. The new species is close to N. yeae, N. daunchina, N. yaoica, and N. chapaensis from southwestern and south-central China and northern Indochina, while the relationships among these species remain unresolved. Nidirana guangxiensis sp. nov. can be distinguished from all known congeners by the genetic divergences in the mitochondrial 16S and COI genes, the behavior of nest construction, the advertisement call containing 6–11 rapidly repeated regular notes, and a combination of morphological characteristics. Furthermore, the Nidirana populations recorded in Guangxi are clarified in this work, providing valuable new information on the knowledge of the genus Nidirana.
Keywords	Ranidae ; frogs ; mitochondria ; music ; nests ; new species ; China ; Indochina
Language	English
Dates of publication	2021-0901
Size	p. 35-56.
Publishing place	Pensoft Publishers
Document type	Article
ZDB-ID	2445640-8
ISSN	1313-2970 ; 1313-2989
ISSN (online)	1313-2970
ISSN	1313-2989
DOI	10.3897/zookeys.1059.68140
Database	NAL-Catalogue (AGRICOLA)

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Article: Paleochannel-controlled earth fissures in Daming, North China Plain and their implication for underground paleogeomorphology

Xu, Jishan / Baoting Yang / Feiyong Wang / Haibo An / Haiyu Sun / Hongwei Hu / Jianbing Peng

Geomorphology. 2019 Feb. 15, v. 327

2019

Abstract: ... developed. Among them, 29 fissures are evolved in the Daming zone in the upper reaches of the paleochannel ... than 10 m) and good correspondence to Daming paleochannels. These basic characteristics reveal that unlike ... other tectonic earth fissures, Daming earth fissures are a new and unknown type of fissures, i.e., paleochannel ...

Abstract	Earth fissures are a geologic phenomenon that mainly affects the medium with shallow surface soil and mainly developed in the Quaternary sediment cover. In the Yellow River-Qinghe River - Zhanghe River shallow buried paleochannel Band (HQZ-Qh2-II Band) on the North China Plain, 93 earth fissures are developed. Among them, 29 fissures are evolved in the Daming zone in the upper reaches of the paleochannel band. Thus, the zone belongs to a densely grouped fissure area. This study, through a series of field trenching and surveying, revealed that these earth fissures have unique plane distribution rules and profile structure features of short length (several meters to several hundred meters), shallow depth (smaller than 10 m) and good correspondence to Daming paleochannels. These basic characteristics reveal that unlike other tectonic earth fissures, Daming earth fissures are a new and unknown type of fissures, i.e., paleochannel-controlled earth fissures. Theoretical analysis of their features showed that they were formed due to the actions of gravity, primary joint factors and groundwater pumping activities and controlled by ancient river factors including paleochannels, sediments and groundwater. Among them, the process of Mode I can be summed up as “self-gravity action - tensile cracks - surface water scouring - earth fissures”. The process of Mode II is “primary join - surface water infiltration - piping - cave in - earth fissures”. The process of Mode III is “groundwater extraction - aquifer compression - inhomogeneous ground subsidence - earth fissures”. These formation mechanisms of paleochannel-induced earth fissures can be used to predict the fine distribution of shallow buried paleochannels and the overall migration process of the rivers. Thus, this kind of earth fissures is of great significance for understanding the underground paleogeomorphology (buried paleochannel) of the North China Plain and other alluvial plains of the world.
Keywords	alluvial plains ; aquifers ; gravity ; groundwater ; groundwater extraction ; infiltration (hydrology) ; rivers ; sediments ; soil ; subsidence ; surface water ; surveys ; tectonics ; trenching ; China
Language	English
Dates of publication	2019-0215
Size	p. 523-532.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	58028-4
ISSN	0169-555X
ISSN	0169-555X
DOI	10.1016/j.geomorph.2018.11.020
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Daming capsule restores endothelial dysfunction induced by high-fat diet.

Zhang, Rong / Niu, Huifang / Wang, Ning / Sun, Lihua / Xu, Yi / Zhao, Ruibo / Ban, Xiang / Yu, Yao / Yang, Baofeng / Ai, Jing

BMC complementary and alternative medicine

2012 Volume 12, Page(s) 21

Abstract: Background: Daming capsule (DMC), a traditional Chinese formula, has a lipid-modulating action ...

Abstract	Background: Daming capsule (DMC), a traditional Chinese formula, has a lipid-modulating action with reduced adverse side effects as compared with other lipid lowering compounds. Since endothelial dysfunction often accompanies the hyperlipidemic state, we hypothesize that DMC might restore endothelial dysfunction produced by a high-fat (HF) diet. Importantly, we also investigate possible mechanisms involved in mediating the effects of DMC on vascular reactivity. Methods: Rats were divided into four groups: control, HF diet, HF mixed DMC diet, HF mixed atorvastatin (ATV) diet. After 30 days, the thoracic cavity was exposed to remove the thoracic aorta for (i) histological examination; (ii) measurement of endothelial nitric oxide synthase (eNOS) by western blot; and (iii) tension study of thoracic aortic ring. Results: HF diet induced significant attenuation in the contraction and relaxation of rat aortic rings. Treatment with DMC significantly improved the relaxation of the aortic rings as compared with those from HF rats (P < 0.05), which was abolished by a nonspecific NOS inhibitor L-NAME. Moreover DMC significantly restored the decrease in eNOS expression induced by HF diet. Similar results were found in histopathologic changes. DMC failed to restore the loss of vasocontraction of aorta explained by an impairment of ATP-sensitive K+ channels (KATP) on the structure and/or function. DMC exerted the same protective effect as ATV, a positive control drug, on vascular injury produced by HF diet. Conclusion: DMC partially protects the aorta from HF-induced endothelial dysfunction via upregulation of the expression of eNOS.
MeSH term(s)	Animals ; Anticholesteremic Agents/therapeutic use ; Aorta, Thoracic/drug effects ; Atorvastatin ; Diet, High-Fat/adverse effects ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Endothelium, Vascular/drug effects ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/physiopathology ; Enzyme Inhibitors/pharmacology ; Heptanoic Acids/therapeutic use ; Hyperlipidemias/complications ; Hyperlipidemias/drug therapy ; Hypolipidemic Agents/pharmacology ; Hypolipidemic Agents/therapeutic use ; Male ; NG-Nitroarginine Methyl Ester/pharmacology ; Nitric Oxide Synthase/metabolism ; Phytotherapy ; Potassium Channels/drug effects ; Pyrroles/therapeutic use ; Rats ; Rats, Wistar ; Vascular Diseases/metabolism ; Vascular Diseases/physiopathology ; Vascular Diseases/prevention & control ; Vasoconstriction/drug effects ; Vasodilation/drug effects
Chemical Substances	Anticholesteremic Agents ; Drugs, Chinese Herbal ; Enzyme Inhibitors ; Heptanoic Acids ; Hypolipidemic Agents ; Potassium Channels ; Pyrroles ; Atorvastatin (A0JWA85V8F) ; Nitric Oxide Synthase (EC 1.14.13.39) ; NG-Nitroarginine Methyl Ester (V55S2QJN2X)
Language	English
Publishing date	2012-03-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2050429-9
ISSN	1472-6882 ; 1472-6882
ISSN (online)	1472-6882
ISSN	1472-6882
DOI	10.1186/1472-6882-12-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Daming capsule restores endothelial dysfunction induced by high-fat diet

Zhang Rong / Niu Huifang / Wang Ning / Sun Lihua / Xu Yi / Zhao Ruibo / Ban Xiang / Yu Yao / Yang Baofeng / Ai Jing

BMC Complementary and Alternative Medicine, Vol 12, Iss 1, p

2012 Volume 21

Abstract: Abstract Background Daming capsule (DMC), a traditional Chinese formula, has a lipid-modulating ...

Abstract	Abstract Background Daming capsule (DMC), a traditional Chinese formula, has a lipid-modulating action with reduced adverse side effects as compared with other lipid lowering compounds. Since endothelial dysfunction often accompanies the hyperlipidemic state, we hypothesize that DMC might restore endothelial dysfunction produced by a high-fat (HF) diet. Importantly, we also investigate possible mechanisms involved in mediating the effects of DMC on vascular reactivity. Methods Rats were divided into four groups: control, HF diet, HF mixed DMC diet, HF mixed atorvastatin (ATV) diet. After 30 days, the thoracic cavity was exposed to remove the thoracic aorta for (i) histological examination; (ii) measurement of endothelial nitric oxide synthase (eNOS) by western blot; and (iii) tension study of thoracic aortic ring. Results HF diet induced significant attenuation in the contraction and relaxation of rat aortic rings. Treatment with DMC significantly improved the relaxation of the aortic rings as compared with those from HF rats ( P < 0.05), which was abolished by a nonspecific NOS inhibitor L-NAME. Moreover DMC significantly restored the decrease in eNOS expression induced by HF diet. Similar results were found in histopathologic changes. DMC failed to restore the loss of vasocontraction of aorta explained by an impairment of ATP-sensitive K + channels (K ATP ) on the structure and/or function. DMC exerted the same protective effect as ATV, a positive control drug, on vascular injury produced by HF diet. Conclusion DMC partially protects the aorta from HF-induced endothelial dysfunction via upregulation of the expression of eNOS.
Keywords	Other systems of medicine ; RZ201-999
Subject code	630
Language	English
Publishing date	2012-03-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Daming capsule restores endothelial dysfunction induced by high-fat diet

Zhang, Rong / Huifang Niu / Ning Wang / Lihua Sun / Yi Xu / Ruibo Zhao / Xiang Ban / Yao Yu / Baofeng Yang / Jing Ai

BMC complementary and alternative medicine. 2012 Dec., v. 12, no. 1

2012

Abstract: BACKGROUND: Daming capsule (DMC), a traditional Chinese formula, has a lipid-modulating action ...

Abstract	BACKGROUND: Daming capsule (DMC), a traditional Chinese formula, has a lipid-modulating action with reduced adverse side effects as compared with other lipid lowering compounds. Since endothelial dysfunction often accompanies the hyperlipidemic state, we hypothesize that DMC might restore endothelial dysfunction produced by a high-fat (HF) diet. Importantly, we also investigate possible mechanisms involved in mediating the effects of DMC on vascular reactivity. METHODS: Rats were divided into four groups: control, HF diet, HF mixed DMC diet, HF mixed atorvastatin (ATV) diet. After 30 days, the thoracic cavity was exposed to remove the thoracic aorta for (i) histological examination; (ii) measurement of endothelial nitric oxide synthase (eNOS) by western blot; and (iii) tension study of thoracic aortic ring. RESULTS: HF diet induced significant attenuation in the contraction and relaxation of rat aortic rings. Treatment with DMC significantly improved the relaxation of the aortic rings as compared with those from HF rats (P < 0.05), which was abolished by a nonspecific NOS inhibitor L-NAME. Moreover DMC significantly restored the decrease in eNOS expression induced by HF diet. Similar results were found in histopathologic changes. DMC failed to restore the loss of vasocontraction of aorta explained by an impairment of ATP-sensitive Kâº channels (KATP) on the structure and/or function. DMC exerted the same protective effect as ATV, a positive control drug, on vascular injury produced by HF diet. CONCLUSION: DMC partially protects the aorta from HF-induced endothelial dysfunction via upregulation of the expression of eNOS.
Keywords	Western blotting ; adverse effects ; alternative medicine ; aorta ; drugs ; endothelial nitric oxide synthase ; high fat diet ; histology ; histopathology ; lipids ; potassium channels ; protective effect ; rats ; thoracic cavity
Language	English
Dates of publication	2012-12
Size	p. 21.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2050429-9
ISSN	1472-6882
ISSN	1472-6882
DOI	10.1186/1472-6882-12-21
Database	NAL-Catalogue (AGRICOLA)

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Article: [Effect of Daming capsule on expression of connexin43 isoforms in hyperlipemic rat's cardiac muscle].

Xing, Yan / Yue, Peng / Sun, Li-hua / Zhao, Wen-xiao / Wang, Ying / Zhang, Yong / Liu, Xin / Lu, Yan-jie / Yang, Bao-feng

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2007 Volume 32, Issue 14, Page(s) 1440–1445

Abstract: ... Daming capsule group of high dose, middle dose and low dose (200, 100, 50 mg kg(-1) d(-1)). Each group ... P <0. 05). After treating with Daming capsule, the concentration of the preceding four indexes were ... established and Daming capsule has the effect of lowering blood lipid. Furthermore, the molecular mechanism ...

Abstract	Objective: To find the molecular mechanism of decreasing blood fat effect of Darning capsule on hyperlipemic rat, we study the expression of connexin43 in the myocardium before and after using the capsule. Method: Forty Wistar rats were randomly divided into 5 group: control group, hyperlipemia model group, Daming capsule group of high dose, middle dose and low dose (200, 100, 50 mg kg(-1) d(-1)). Each group had 8 rats. Hyperlipemic rat model was made firstly, the blood was obtained via vena caudalis and the indexes of TC, TG, LDL, HDL and NEFA in the serum were measured. The myocardial total RNA was extracted by Trizol method. To compare the expression of connexin43 in the following groups: hyperlipemia, normal and drug, we used the technique of RT-PCR, immunostaining and microconfoul. Result: The concentrations of TC, TG, LDL and NEFA in hyperlipemic serum were increased (P <0. 05), while that of HDL was decreased (P <0. 05). After treating with Daming capsule, the concentration of the preceding four indexes were decreased and the concentrations of HDL was increased up to nearly normal level. No significant difference was found in the ECG of the three groups. As compared with the normal group, the mRNA expressions of connexin43 in hyperlipemia group was weakened (P <0.05), while that of the drug group was enhanced(P <0.05). The same result in immunostaining was observed. Conclusion: Hyperlipemic rat model was successfully established and Daming capsule has the effect of lowering blood lipid. Furthermore, the molecular mechanism of Darning capsule is related with the change of Cx43 closely.
MeSH term(s)	Animals ; Capsules ; Cholesterol/blood ; Connexin 43/biosynthesis ; Connexin 43/genetics ; Drugs, Chinese Herbal/isolation & purification ; Drugs, Chinese Herbal/pharmacology ; Fatty Acids, Nonesterified/blood ; Hyperlipidemias/blood ; Hyperlipidemias/metabolism ; Hypolipidemic Agents/administration & dosage ; Hypolipidemic Agents/pharmacology ; Male ; Myocardium/metabolism ; Plants, Medicinal/chemistry ; Protein Isoforms/biosynthesis ; Protein Isoforms/genetics ; RNA, Messenger/biosynthesis ; Random Allocation ; Rats ; Rats, Wistar ; Triglycerides/blood
Chemical Substances	Capsules ; Connexin 43 ; Drugs, Chinese Herbal ; Fatty Acids, Nonesterified ; Hypolipidemic Agents ; Protein Isoforms ; RNA, Messenger ; Triglycerides ; Cholesterol (97C5T2UQ7J)
Language	Chinese
Publishing date	2007-07
Publishing country	China
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Protective effect of the daming capsule on impaired baroreflexes in STZ-induced diabetic rats with hyperlipoidemia.

Ai, Jing / Wang, Li-Hong / Zhang, Rong / Qiao, Guo-Fen / Wang, Ning / Sun, Li-Hua / Lu, Guan-Yi / Sun, Chao / Yang, Bao-Feng

BMC complementary and alternative medicine

2010 Volume 10, Page(s) 80

Abstract: Background: The Daming capsule (DMC) is a traditional Chinese medicine used to treat ... receptor expression.: Conclusion: The Daming capsule partially reversed the parasympathetic baroreflex ...

Abstract	Background: The Daming capsule (DMC) is a traditional Chinese medicine used to treat hyperlipoidemia. Both clinic trials and studies on animal models have demonstrated that DMC is beneficial against diabetic symptoms. Impairment of the baroreflex can cause life-threatening arrhythmias and sudden cardiac death in patients with diabetes mellitus (DM). This study was designed to elucidate the effects of DMC on baroreflexes in streptozocin (STZ)-induced diabetic rats with hyperlipoidemia. Methods: Wistar rats were randomly divided into three groups: untreated controls, rats pretreated STZ and high lipids (a diabetes model or DM rats), and DM rats treated with DMC. The baroreflex sensitivity was examined during intravenous injection of phenylephrine (PE) or sodium nitroprusside (SNP) and quantified by the change in heart rate over the change in mean arterial blood pressure (ΔHR/ΔMABP). Morphological remodeling of baroreceptors was analyzed by transmission electron microscopy (TEM). The mRNA levels and expression of GluR2 and a GABAA receptor subunit were measured by quantitative RT-PCR and Western blotting. Results: Compared to untreated DM rats, DMC significantly elevated the ratio of ΔHR/ΔMABP by enhancing the compensatory reduction in HR (-ΔHR) in response to PE-induced hypertension (+ΔMABP) (P < 0.05). In the presence of SNP, DMC increased the ΔMABP (P < 0.05). In addition, DMC markedly shortened the duration of blood pressure changes elicited by PE or SNP in DM rats compared to the untreated DM group (P < 0.05). Electron microscopy revealed disrupted myelin sheaths, swollen ER, and lysed mitochondria in the nucleus ambiguous (NAm) DM rats. These signs of neuropathology were largely prevented by treatment with DMC for 30 days. Treatment with DMC elevated both mRNA and protein level of GluR2 in the NAm of DM rats, but had no effect on GABAA receptor expression. Conclusion: The Daming capsule partially reversed the parasympathetic baroreflex impairment observed in STZ-induced diabetic rats with hyperlipoidemia. Treatment with DMC also prevented the degeneration of neurons and myelinated axons in the brain stem NAm and reversed the down-regulation of GluR2 mRNA. Rescue of NAm function may contribute to the medicinal properties of DMC in diabetic rats.
MeSH term(s)	Animals ; Baroreflex/drug effects ; Baroreflex/physiology ; Blood Pressure/drug effects ; Brain/drug effects ; Brain/pathology ; Cassia ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/metabolism ; Diabetes Mellitus, Experimental/physiopathology ; Dietary Fats/adverse effects ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Endoplasmic Reticulum/drug effects ; Heart Rate/drug effects ; Hyperlipidemias/drug therapy ; Hyperlipidemias/metabolism ; Hyperlipidemias/physiopathology ; Hypertension/chemically induced ; Male ; Mitochondria/drug effects ; Myelin Sheath/drug effects ; Nitroprusside/pharmacology ; Panax ; Phenylephrine/pharmacology ; Phytotherapy ; Pressoreceptors/drug effects ; Pressoreceptors/pathology ; RNA, Messenger/metabolism ; Random Allocation ; Rats ; Rats, Wistar ; Receptors, AMPA/genetics ; Receptors, AMPA/metabolism ; Receptors, GABA-A/metabolism ; Rheum ; Salvia miltiorrhiza
Chemical Substances	Dietary Fats ; Drugs, Chinese Herbal ; RNA, Messenger ; Receptors, AMPA ; Receptors, GABA-A ; Nitroprusside (169D1260KM) ; Phenylephrine (1WS297W6MV) ; glutamate receptor ionotropic, AMPA 2 (P6W5IXV8V9)
Language	English
Publishing date	2010-12-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2050429-9
ISSN	1472-6882 ; 1472-6882
ISSN (online)	1472-6882
ISSN	1472-6882
DOI	10.1186/1472-6882-10-80
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Protective effect of the daming capsule on impaired baroreflexes in STZ-induced diabetic rats with hyperlipoidemia

Lu Guan-Yi / Sun Li-Hua / Wang Ning / Qiao Guo-Fen / Zhang Rong / Wang Li-Hong / Ai Jing / Sun Chao / Yang Bao-Feng

BMC Complementary and Alternative Medicine, Vol 10, Iss 1, p

2010 Volume 80

Abstract: Abstract Background The Daming capsule (DMC) is a traditional Chinese medicine used to treat ... A receptor expression. Conclusion The Daming capsule partially reversed the parasympathetic baroreflex ...

Abstract	Abstract Background The Daming capsule (DMC) is a traditional Chinese medicine used to treat hyperlipoidemia. Both clinic trials and studies on animal models have demonstrated that DMC is beneficial against diabetic symptoms. Impairment of the baroreflex can cause life-threatening arrhythmias and sudden cardiac death in patients with diabetes mellitus (DM). This study was designed to elucidate the effects of DMC on baroreflexes in streptozocin (STZ)-induced diabetic rats with hyperlipoidemia. Methods Wistar rats were randomly divided into three groups: untreated controls, rats pretreated STZ and high lipids (a diabetes model or DM rats), and DM rats treated with DMC. The baroreflex sensitivity was examined during intravenous injection of phenylephrine (PE) or sodium nitroprusside (SNP) and quantified by the change in heart rate over the change in mean arterial blood pressure (ΔHR/ΔMABP). Morphological remodeling of baroreceptors was analyzed by transmission electron microscopy (TEM). The mRNA levels and expression of GluR2 and a GABA A receptor subunit were measured by quantitative RT-PCR and Western blotting. Results Compared to untreated DM rats, DMC significantly elevated the ratio of ΔHR/ΔMABP by enhancing the compensatory reduction in HR (-ΔHR) in response to PE-induced hypertension (+ΔMABP) ( P < 0.05). In the presence of SNP, DMC increased the ΔMABP ( P < 0.05). In addition, DMC markedly shortened the duration of blood pressure changes elicited by PE or SNP in DM rats compared to the untreated DM group ( P < 0.05). Electron microscopy revealed disrupted myelin sheaths, swollen ER, and lysed mitochondria in the nucleus ambiguous (NAm) DM rats. These signs of neuropathology were largely prevented by treatment with DMC for 30 days. Treatment with DMC elevated both mRNA and protein level of GluR2 in the NAm of DM rats, but had no effect on GABA A receptor expression. Conclusion The Daming capsule partially reversed the parasympathetic baroreflex impairment observed in STZ-induced diabetic rats with ...
Keywords	Other systems of medicine ; RZ201-999
Subject code	610
Language	English
Publishing date	2010-12-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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