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  1. Article ; Online: Solution structure and RNA-binding of a minimal ProQ-homolog from

    Immer, Carina / Hacker, Carolin / Wöhnert, Jens

    RNA (New York, N.Y.)

    2020  Volume 26, Issue 12, Page(s) 2031–2043

    Abstract: Small regulatory RNAs (sRNAs) play an important role for posttranscriptional gene regulation in bacteria. sRNAs recognize their target messenger RNAs (mRNAs) by base-pairing, which is often facilitated by interactions with the bacterial RNA-binding ... ...

    Abstract Small regulatory RNAs (sRNAs) play an important role for posttranscriptional gene regulation in bacteria. sRNAs recognize their target messenger RNAs (mRNAs) by base-pairing, which is often facilitated by interactions with the bacterial RNA-binding proteins Hfq or ProQ. The FinO/ProQ RNA-binding protein domain was first discovered in the bacterial repressor of conjugation, FinO. Since then, the functional role of FinO/ProQ-like proteins in posttranscriptional gene regulation was extensively studied in particular in the enterobacteria
    MeSH term(s) Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Legionella pneumophila/genetics ; Legionella pneumophila/metabolism ; Nuclear Magnetic Resonance, Biomolecular/methods ; Protein Binding ; Protein Domains ; RNA, Bacterial/chemistry ; RNA, Bacterial/metabolism ; RNA-Binding Proteins/chemistry ; RNA-Binding Proteins/metabolism ; Structure-Activity Relationship
    Chemical Substances Bacterial Proteins ; RNA, Bacterial ; RNA-Binding Proteins
    Language English
    Publishing date 2020-09-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1241540-6
    ISSN 1469-9001 ; 1355-8382
    ISSN (online) 1469-9001
    ISSN 1355-8382
    DOI 10.1261/rna.077354.120
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4777: Show issues Location:
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  2. Article ; Online: NMR resonance assignments for a ProQ homolog from Legionella pneumophila.

    Immer, Carina / Hacker, Carolin / Wöhnert, Jens

    Biomolecular NMR assignments

    2018  Volume 12, Issue 2, Page(s) 319–322

    Abstract: Regulation of gene expression on a post-transcriptional level by small non-coding regulatory RNAs (sRNAs) is very common in bacteria. sRNAs base pair with sequences in their target messenger RNAs (mRNAs) and thereby regulate translation initiation or ... ...

    Abstract Regulation of gene expression on a post-transcriptional level by small non-coding regulatory RNAs (sRNAs) is very common in bacteria. sRNAs base pair with sequences in their target messenger RNAs (mRNAs) and thereby regulate translation initiation or mRNA stability. Specialized RNA-binding proteins (RBPs) facilitate these regulatory sRNA/mRNA interactions by acting as RNA chaperones. A well-known example for such an RNA chaperone which is widespread in bacteria is the Hfq protein. Recently, the ProQ/FinO protein family was identified as a new class of RNA chaperones involved in sRNA based regulation. Only a few members of this protein family have been structurally characterized so far. In particular, the structural basis for RNA-binding and recognition has not yet been established for this class of proteins. Here, we report the
    MeSH term(s) Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Legionella pneumophila ; Nuclear Magnetic Resonance, Biomolecular ; RNA/metabolism ; RNA-Binding Proteins/chemistry ; RNA-Binding Proteins/metabolism ; Sequence Homology, Amino Acid
    Chemical Substances Bacterial Proteins ; RNA-Binding Proteins ; RNA (63231-63-0)
    Language English
    Publishing date 2018-06-22
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2388861-1
    ISSN 1874-270X ; 1874-2718
    ISSN (online) 1874-270X
    ISSN 1874-2718
    DOI 10.1007/s12104-018-9831-6
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  3. Article ; Online: Which Factors Determine Our Online Social Capital? An Analysis Based on Structural Equation Modelling

    Michael Grottke / Janine Viol Hacker / Carolin Durst

    Australasian Journal of Information Systems, Vol 22, Iss

    2018  

    Abstract: The relationship between social network sites and social capital has received much research attention. However, two research gaps can be identified in the existing literature. First, only few studies have examined online social capital as a resource in ... ...

    Abstract The relationship between social network sites and social capital has received much research attention. However, two research gaps can be identified in the existing literature. First, only few studies have examined online social capital as a resource in online social networks. In this regard, it is not clear how to validly measure online social capital. Second, while the factors influencing social capital, among them properties of an individual’s social network, have been investigated in offline settings, such factors have not yet been investigated in terms of online social capital. Addressing these gaps, we asked 1000 Facebook users to provide information on their Facebook usage and online friendship network. Employing structural equation modelling for analysing the survey data, we show that Williams’ Internet Social Capital Scales, which are commonly used to assess social capital in offline settings, can be used to validly measure online social capital. Moreover, we find that some of the variables influencing offline social capital, among them similarity in terms of sociodemographic attributes, seem less important in an online setting.
    Keywords Social capital ; Social networks ; Structural modeling / Structural equation modeling (SEM) ; Information technology ; T58.5-58.64 ; Electronic computers. Computer science ; QA75.5-76.95
    Subject code 303
    Language English
    Publishing date 2018-07-01T00:00:00Z
    Publisher Australasian Association for Information Systems
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: 1

    Kaiser, Marco / Hacker, Carolin / Duchardt-Ferner, Elke / Wöhnert, Jens

    Biomolecular NMR assignments

    2019  Volume 13, Issue 2, Page(s) 309–314

    Abstract: The protein dimethyladenosine transferase 1 (Dim1) is a highly conserved protein occurring in organisms ranging from bacteria such as E. coli where it is named KsgA to humans. Since Dim1 is involved in the biogenesis of the small ribosomal subunit it is ... ...

    Abstract The protein dimethyladenosine transferase 1 (Dim1) is a highly conserved protein occurring in organisms ranging from bacteria such as E. coli where it is named KsgA to humans. Since Dim1 is involved in the biogenesis of the small ribosomal subunit it is an essential protein. During ribosome biogenesis Dim1 acts as an rRNA modification enzyme and dimethylates two adjacent adenosine residues of the small ribosomal subunit rRNA. In eukaryotes it is also required to ensure the proper endonucleolytic processing of the small ribosomal subunit rRNA precursor. Recently, a third function was proposed for eukaryotic Dim1. Karbstein and coworkers suggested that Dim1 interacts with the essential ribosome assembly factor Fap7 and that Fap7 is responsible for the dissociation of Dim1 from the nascent small ribosomal subunit. Here, we report the backbone
    MeSH term(s) Methyltransferases/chemistry ; Models, Molecular ; Nuclear Magnetic Resonance, Biomolecular ; Protein Conformation ; Pyrococcus horikoshii/enzymology
    Chemical Substances Methyltransferases (EC 2.1.1.-)
    Language English
    Publishing date 2019-05-08
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2388861-1
    ISSN 1874-270X ; 1874-2718
    ISSN (online) 1874-270X
    ISSN 1874-2718
    DOI 10.1007/s12104-019-09897-8
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  5. Article ; Online: A New Docking Domain Type in the Peptide-Antimicrobial-Xenorhabdus Peptide Producing Nonribosomal Peptide Synthetase from

    Watzel, Jonas / Hacker, Carolin / Duchardt-Ferner, Elke / Bode, Helge B / Wöhnert, Jens

    ACS chemical biology

    2020  Volume 15, Issue 4, Page(s) 982–989

    Abstract: Nonribosomal peptide synthetases (NRPSs) produce a wide variety of different natural products from amino acid precursors. In contrast to single protein NRPS, the NRPS of the ... ...

    Abstract Nonribosomal peptide synthetases (NRPSs) produce a wide variety of different natural products from amino acid precursors. In contrast to single protein NRPS, the NRPS of the bacterium
    MeSH term(s) Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Peptide Synthases/chemistry ; Peptide Synthases/metabolism ; Protein Binding ; Protein Conformation, alpha-Helical ; Protein Interaction Domains and Motifs ; Protein Subunits/chemistry ; Protein Subunits/metabolism ; Xenorhabdus/enzymology
    Chemical Substances Bacterial Proteins ; Protein Subunits ; Peptide Synthases (EC 6.3.2.-) ; non-ribosomal peptide synthase (EC 6.3.2.-)
    Language English
    Publishing date 2020-03-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1554-8937
    ISSN (online) 1554-8937
    DOI 10.1021/acschembio.9b01022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book ; Thesis: Komplikationen bei der peripheren und zentralen Applikation von Chemotherapeutika bei testikulären Keimzelltumoren

    Sautter, Lisa / Häcker, Axel

    2017  

    Institution Universität Heidelberg
    Author's details vorgelegt von Lisa Sarah Sautter, geb. Junkers ; Referent: Prof. Dr. med. Axel Häcker
    Language German
    Size 57 Blätter, Diagramme, 30 cm
    Publishing place Heidelberg
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dissertation, Ruprecht-Karls-Universität zu Heidelberg, 2019
    HBZ-ID HT020242414
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2019 E 1235 order for loan Magazin

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  7. Article ; Online: Can Health 2.0 Address Critical Healthcare Challenges? Insights from the Case of How Online Social Networks Can Assist in Combatting the Obesity Epidemic

    Janine Hacker / Nilmini Wickramasinghe / Carolin Durst

    Australasian Journal of Information Systems, Vol 21, Iss

    2017  

    Abstract: One of the serious concerns in healthcare in this 21st century is obesity. While the causes of obesity are multifaceted, social networks have been identified as one of the most important dimensions of people's social environment that may influence the ... ...

    Abstract One of the serious concerns in healthcare in this 21st century is obesity. While the causes of obesity are multifaceted, social networks have been identified as one of the most important dimensions of people's social environment that may influence the adoption of many behaviours, including health-promoting behaviours. In this article, we examine the possibility of harnessing the appeal of online social networks to address the obesity epidemic currently plaguing society. Specifically, a design science research methodology is adopted to design, implement and test the Health 2.0 application called “Calorie Cruncher”. The application is designed specifically to explore the influence of online social networks on individual’s health-related behaviour. In this regard, pilot data collected based on qualitative interviews indicate that online social networks may influence health-related behaviours in several ways. Firstly, they can influence people’s norms and value system that have an impact on their health-related behaviours. Secondly, social control and pressure of social connections may also shape health-related behaviours, and operate implicitly when people make food selection decisions. Thirdly, social relationships may provide emotional support. Our study has implications for research and practice. From a theoretical perspective, the article inductively identifies three factors that influence specific types of health outcomes in the context of obesity. From a practical perspective, the study underscores the benefits of adopting a design science methodology to design and implement a technology solution for a healthcare issue as well as the key role for online social media to assist with health and wellness management and maintenance.
    Keywords Online Social Networks ; Health 2.0 Application ; Design Science Methodology ; Obesity ; Information technology ; T58.5-58.64 ; Electronic computers. Computer science ; QA75.5-76.95
    Subject code 360
    Language English
    Publishing date 2017-03-01T00:00:00Z
    Publisher Australasian Association for Information Systems
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: Messung der Customer Experience im Ladengeshcäft mit Location-based Crowdsourcing und Geofencing

    Durst, Carolin / Berthelmann, Theresa / Hacker, Janine

    HMD : Praxis der Wirtschaftsinformatik Vol. 54, No. 317 , p. 713-726

    Customer experience measurement at the point of sale using location-based crowdsourcing and geofencing

    2017  Volume 54, Issue 317, Page(s) 713–726

    Author's details Carolin Durst, Janine Hacker, Theresa Berthelmann
    Keywords Crowdsourcing ; Geofencing ; Location-based Crowdsourcing ; Customer-Experience-Messung
    Language German
    Publisher Springer Vieweg
    Publishing place Wiesbaden
    Document type Article
    Note Zusammenfassung in englischer Sprache ; Literaturangaben
    ZDB-ID 1015731-1 ; 881938-5
    ISSN 0723-5208
    ISSN 0723-5208
    Database ECONomics Information System

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    In stock of ZB MED Bonn / Germany

    4' 72/5072: Show issues

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  9. Article ; Online: A Single-Center 10-Year Experience of 180 Transmasculine Patients Undergoing Gender-Affirming Mastectomy While Continuing Masculinizing Hormone Replacement Therapy.

    Ederer, Ines Ana / Spennato, Stefano / Nguyen, Cam-Tu / Wehle, Andrej / Wachtel, Carolin / Kiehlmann, Marcus / Hacker, Stefan / Kueenzlen, Lara / Kuehn, Shafreena / Rothenberger, Jens / Rieger, Ulrich M

    Aesthetic plastic surgery

    2022  Volume 47, Issue 3, Page(s) 946–954

    Abstract: Background: Gender-affirming mastectomy is a fundamental step in the transition process of transmasculine patients following the initiation of hormone replacement therapy. Its perioperative management, however, remains underreported and controversial. ... ...

    Abstract Background: Gender-affirming mastectomy is a fundamental step in the transition process of transmasculine patients following the initiation of hormone replacement therapy. Its perioperative management, however, remains underreported and controversial. In this study, a large series of mastectomies in transmen maintaining hormonal therapy is presented.
    Methods: Over a 10-year study period, a consecutive series of 180 transmasculine patients undergoing chest masculinizing surgery was evaluated. Demographical and surgical data were collected and analyzed for potential factors influencing outcome.
    Results: The overall rate of complications was 15.5%. Patients who underwent periareolar incision mastectomy were significantly more likely to develop any type of complication than patients with a sub-mammary incision (28.6% vs. 13.2%, p = 0.045). Hematoma was the most common reason for surgical revision. It occurred significantly more often among the periareolar group (21.4% vs. 7.9%, p = 0.041). Duration and type of hormonal therapy did not differ between patients with or without complications. In a multivariate regression analysis, smoking and type of incision were identified as significant predictors of the all-cause complication rate, whereas the influence of BMI and resection weight diminished after adjusting for confounding factors.
    Conclusion: There is scarcity of information concerning the influence of perioperative hormonal therapy in patients undergoing chest wall masculinization. The observed complication rates-with special regard to hematoma-were comparable to current reports; yet further research is needed to profoundly evaluate this topic and provide evidence-based recommendations for the perioperative management of HRT of transmasculine patients.
    Level of evidence iv: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors http://www.springer.com/00266 .
    MeSH term(s) Humans ; Female ; Mastectomy ; Breast Neoplasms/drug therapy ; Breast Neoplasms/surgery ; Retrospective Studies ; Mammaplasty ; Hormone Replacement Therapy/adverse effects ; Hematoma ; Treatment Outcome
    Language English
    Publishing date 2022-12-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 532791-x
    ISSN 1432-5241 ; 0364-216X
    ISSN (online) 1432-5241
    ISSN 0364-216X
    DOI 10.1007/s00266-022-03213-x
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    In stock of ZB MED Cologne/Königswinter

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  10. Article ; Online: NMR assignments of a dynamically perturbed and dimerization inhibited N-terminal domain variant of a spider silk protein from E. australis.

    Goretzki, Benedikt / Heiby, Julia C / Hacker, Carolin / Neuweiler, Hannes / Hellmich, Ute A

    Biomolecular NMR assignments

    2019  Volume 14, Issue 1, Page(s) 67–71

    Abstract: Web spiders use specialized glands to produce silk proteins, so-called spidroins, which assemble into extraordinarily tough silk fibers through tightly regulated phase and structural transitions. A crucial step in the polymerization of spidroins is the ... ...

    Abstract Web spiders use specialized glands to produce silk proteins, so-called spidroins, which assemble into extraordinarily tough silk fibers through tightly regulated phase and structural transitions. A crucial step in the polymerization of spidroins is the pH-triggered assembly of their N-terminal domains (NTDs) into tight dimers. Major ampullate spidroin NTDs contain an unusually high content of the amino acid methionine. We previously showed that the simultaneous mutation of the six hydrophobic core methionine residues to leucine in the NTD of the major ampullate spidroin 1 from Euprosthenops australis, a nursery web spider, yields a protein (L6-NTD) retaining a three-dimensional fold identical to the wildtype (WT) domain, yet with a significantly increased stability. Further, the dynamics of the L6-NTD are significantly reduced and the ability to dimerize is severely impaired compared to the WT domain. These properties lead to significant changes in the NMR spectra between WT and L6-NTD so that the previously available WT-NTD assignments cannot be transferred to the mutant protein. Here, we thus report the de novo NMR backbone and side chain assignments of the major ampullate spidroin 1 L6-NTD variant from E. australis as a prerequisite for obtaining further insights into protein structure and dynamics.
    MeSH term(s) Animals ; Fibroins/chemistry ; Nuclear Magnetic Resonance, Biomolecular ; Protein Domains ; Protein Multimerization ; Proton Magnetic Resonance Spectroscopy ; Spiders/metabolism
    Chemical Substances spidroin 1 ; Fibroins (9007-76-5)
    Language English
    Publishing date 2019-11-30
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2388861-1
    ISSN 1874-270X ; 1874-2718
    ISSN (online) 1874-270X
    ISSN 1874-2718
    DOI 10.1007/s12104-019-09922-w
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