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Article: A forensic population database of autosomal STR and X-STR markers in the Qiang ethnic minority of China.

Wang, Zefei / Song, Mengyuan / Lyu, Qiang / Ying, Jun / Wu, Qian / Song, Feng / Jiang, Lanrui / Wei, Xiaowen / Wang, Shuangshuang / Wang, Fei / Zhou, Yuxiang / Song, Xingbo / Luo, Haibo

Heliyon

2023 Volume 9, Issue 11, Page(s) e21823

Abstract: The Qiang ethnic group is one of the oldest ethnic groups in China and is the most active ethnic ... nationally and internationally. The paternal and maternal genetic feature of the Qiang ethnic group has been ... Microreader™ 19X System) for application in the Qiang population and to elucidate their genetic diversity ...

Abstract	The Qiang ethnic group is one of the oldest ethnic groups in China and is the most active ethnic group among all the populations along the Tibetan-Yi corridor. They have had a profound impact nationally and internationally. The paternal and maternal genetic feature of the Qiang ethnic group has been revealed, leaving the question of the genetic characteristics from autosomes and X chromosome not answered. The aim of this study was to explore the potential of 36 A-STR (Microreader™ 36A ID System) and 19 X-STR (Microreader™ 19X System) for application in the Qiang population and to elucidate their genetic diversity in southwest China. The cumulative probability of exclusion (CPE) for autosomal STRs is 1-1.3814 × 10
Language	English
Publishing date	2023-11-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e21823
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Article ; Online: DNA barcoding of Notopterygii Rhizoma et Radix (Qiang-huo) and identification of adulteration in its medicinal services.

Liu, Zhen-Wen / Zhou, Jing

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2879

Abstract: ... from the Apiaceae family, commonly known as "Qiang-huo", is a widely used herbal medicine. The recent surge ... may become a universal mini-barcode for the quality control of "Qiang-huo", thereby ensuring the safety ...

Abstract	Safety concerns, stemming from the presence of complex and unpredictable adulterants, permeate the entire industrial chain of traditional Chinese medicines (TCMs). The Notopterygii Rhizoma et Radix (NReR) from the Apiaceae family, commonly known as "Qiang-huo", is a widely used herbal medicine. The recent surge in its demand has given rise to a proliferation of counterfeit and substituted products in the market. Traditional identification presents inherent limitations, while DNA mini-barcoding, reliant on sequencing a short-standardized region, has received considerable attention as a new potential means to identify processed medicinal materials. In this study, we constructed a comprehensive Internal Transcribed Spacer 2 (ITS2) matrix encompassing genuine NReR and their commonly found adulterants for the first time. Leveraging this matrix, we conducted a thorough assessment of the genetic profiles and sources of NReR available in the Chinese herbal medicine market. Following established DNA barcoding protocols, the intra-specific genetic divergences within NReR species were found to be lower than the inter-specific genetic divergences from other species. Among the 120 samples that were successfully amplified, ITS2 exhibits an outstanding species-level identification efficiency of 100% when evaluated using both the BLASTN and neighbor-joining (NJ) tree methods. We concluded that ITS2 is a mini-barcode that has shown its potential and may become a universal mini-barcode for the quality control of "Qiang-huo", thereby ensuring the safety of clinical medication.
MeSH term(s)	Drugs, Chinese Herbal ; DNA Barcoding, Taxonomic/methods ; DNA, Plant/genetics ; DNA, Ribosomal Spacer/genetics ; Phylogeny
Chemical Substances	qiang-huo ; Drugs, Chinese Herbal ; DNA, Plant ; DNA, Ribosomal Spacer
Language	English
Publishing date	2024-02-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-53008-0
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Article ; Online: Paternal genetic structure of the Qiang ethnic group in China revealed by high-resolution Y-chromosome STRs and SNPs.

Song, Mengyuan / Wang, Zefei / Lyu, Qiang / Ying, Jun / Wu, Qian / Jiang, Lanrui / Wang, Fei / Zhou, Yuxiang / Song, Feng / Luo, Haibo / Hou, Yiping / Song, Xingbo / Ying, Binwu

Forensic science international. Genetics

2022 Volume 61, Page(s) 102774

Abstract: The Qiang population mainly lived in Beichuan Qiang Autonomous County of Sichuan Province. It is ... one of the nomads in China, distributed along the Minjiang River. The Qiang population was assumed ... STRs) panels to study the Qiang ethnic group to unravel their paternal genetic, forensic and ...

Abstract	The Qiang population mainly lived in Beichuan Qiang Autonomous County of Sichuan Province. It is one of the nomads in China, distributed along the Minjiang River. The Qiang population was assumed to have great affinity with the Han, the largest ethnic group in China, when it refers to the genetic origin. Whereas, it is deeply understudied, especially from the Y chromosome. Here in this study, we used validated high-resolution Y-chromosome single nucleotide polymorphisms (Y-SNPs) and short tandem repeats (Y-STRs) panels to study the Qiang ethnic group to unravel their paternal genetic, forensic and phylogenetic characteristics. A total of 422 male samples of the Qiang ethnic group were genotyped by 233 Y-SNPs and 29 Y-STRs. Haplogroup O-M175 (N = 312) was the most predominant haplogroup in the Qiang ethnic group, followed by D-M174 (N = 32) and C-M130 (N = 32), N-M231 (N = 27), and Q-M242 (N = 15). After further subdivision, O2a-M324 (N = 213) accounted for the majority of haplogroup O. Haplogroup C2b-Z1338 (N = 29), D1a-CTS11577 (N = 30). O2a2b1a1a1-F42 (N = 48), O2a1b1a1a1a-F11 (N = 35), and O2a2b1a1-M117 (N = 21) represented other large terminal haplogroups. The results unveiled that Qiang ethnic group was a population with a high percentage of haplogroup O2a2b1a1a1-F42 (48/422) and O2a1b1a1a1a-F11 (35/422), and O2a2b1a1-M117 (21/422), which has never been reported. Its haplogroup distribution pattern was different from any of the Han populations, implying that the Qiang ethnic group had its unique genetic pattern. Mismatch analysis indicated that the biggest mismatch number in haplogroup O2a2b1a1a1-F42 was 21, while that of haplogroup O2a1b1a1a1a-F11 was 20. The haplotype diversity of the Qiang ethnic group equaled 0.999788, with 392 haplotypes observed, of which 367 haplotypes were unique. The haplogroup diversity of the Qiang ethnic group reached 0.9767, and 53 terminal haplogroups were observed (The haplogroup diversity of the Qiang ethnic group was the highest among Qiang and all Han subgroups, indicating the larger genetic diversity of the Qiang ethnic group.). Haplogroup O2a2b1a1a1-F42 was the most predominant haplogroup, including 11.37 % of the Qiang individuals. Median-joining trees showed gene flow between the Qiang and Han individuals. Our results indicated that 1) the highest genetic diversity was observed in the Qiang ethnic group compared to any of the former studied Chinese population, suggesting that the Qiang might be an older paternal branch; 2) the haplogroup D-M174 individuals of Qiang, Tibetans and Japanese distributed in three different subclades, which was unable to identify through low-resolution Y-SNP panel; and 3) the Qiang had lower proportion of haplogroup D compared to Yi and Tibetan ethnic groups, showing that the Qiang had less genetic communication with them than with Han Chinese.
MeSH term(s)	Humans ; Ethnicity/genetics ; Chromosomes, Human, Y ; Polymorphism, Single Nucleotide ; Genetics, Population ; Phylogeny ; Haplotypes ; Microsatellite Repeats ; China
Language	English
Publishing date	2022-09-13
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2493339-9
ISSN	1878-0326 ; 1872-4973
ISSN (online)	1878-0326
ISSN	1872-4973
DOI	10.1016/j.fsigen.2022.102774
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Zs.A 6764: Show issues

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Article ; Online: Erratum: SHENGBO ZHOU (), LI HE (), SIYU MA (), SHUJUN XU (), QIANG ZHAI (), PING GUAN (), HUI WANG ()amp; JINGSONG SHI () (2022)Taxonomic status of Rana nigromaculata mongolia and the validity of Pelophylax tenggerensis (Anura, Ranidae) Zootaxa 5165 (4): 486500.

Zhou, Shengbo / He, L I / Ma, Siyu / Xu, Shujun / Zhai, Qiang / Guan, Ping / Wang, Hui / Shi, Jingsong

Zootaxa

2022 Volume 5174, Issue 5, Page(s) 600

Language	English
Publishing date	2022-08-12
Publishing country	New Zealand
Document type	Journal Article
ISSN	1175-5334
ISSN (online)	1175-5334
DOI	10.11646/zootaxa.5174.5.8
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Article ; Online: Tibetan medicine Si-Wei-Qiang-Wei Powder ameliorates cholecystitis via inhibiting the production of pro-inflammatory cytokines and regulating the MAPK signaling pathway.

Zheng, Zhe / Xiong, Hui / Zhao, Zhongqiu / Zhou, Keli / Fu, Miao / Liu, Xinqiao / Mei, Zhinan

Journal of ethnopharmacology

2022 Volume 303, Page(s) 116026

Abstract: Ethnopharmacological relevance: Si-Wei-Qiang-Wei Powder (SWQ) is a formulated ...

Abstract	Ethnopharmacological relevance: Si-Wei-Qiang-Wei Powder (SWQ) is a formulated traditional Tibetan medicine preparation that has been used clinically to treat liver and gallbladder diseases for centuries. Previous work has confirmed its clinical effectiveness, however, the specific mechanism of SWQ is still unknown. Aim of the study: This study aims to explore the anti-inflammatory effect of SWQ on cholecystitis and its possible mechanism. Materials and methods: The main chemical components of SWQ were analyzed by HPLC. The network pharmacology database was used to screen and construct the network of the main components and molecular targets of SWQ, and to predict the molecular pathways of its core targets. Cholecystitis guinea pig model and LPS stimulated cultured human gallbladder epithelial cells (HGBEC) were used, as in vivo and in vitro methods respectively, to study the anti-cholecystitis activity of SWQ. Specifically, gallbladder wall thickness, hematoxylin-eosin (H&E) staining, and liver function indexes were used to evaluate the anti-inflammatory activities of SWQ in cholecystitis; qRT-PCR and ELISA were used to detect the changes of the production of inflammatory cytokines; Western blot analysis was used to analyze the effects of SWQ on phosphorylation of P38, ERK1/2, JNK and AKT. Results: SWQ decreased the indexes of ALT, AST, TBA, CHOL, DBIL in serum and TBIL, TC and Ca Conclusion: In summary, our research showed that SWQ relieves gallbladder inflammation by inhibiting the MAPK pathway.
Language	English
Publishing date	2022-12-09
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.116026
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Zs.A 1494: Show issues

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Article ; Online: Homotherapy for heteropathy active components and mechanisms of Qiang-Huo-Sheng-Shi decoction for treatment of rheumatoid arthritis and osteoarthritis.

Shi, Yuan-Yuan / Li, Ying-Qi / Xie, Xiang / Zhou, Yu-Ting / Zhang, Qian / Yu, Jia-Lin / Li, Ping / Mi, Na / Li, Fei

Computational biology and chemistry

2020 Volume 89, Page(s) 107397

Abstract: Qiang-Huo-Sheng-Shi decoction (QHSSD), a classic traditional Chinese herbal formula, which has been ...

Abstract	Qiang-Huo-Sheng-Shi decoction (QHSSD), a classic traditional Chinese herbal formula, which has been reported to be effective in rheumatoid arthritis (RA) and osteoarthritis (OA). However, the concurrent targeting mechanism of how the aforementioned formula is valid in the two distinct diseases OA and RA, which represents the homotherapy-for-heteropathy principle in traditional Chinese medicine (TCM), have not yet been clarified. In the present study, network pharmacology was adopted to analyze the potential molecular mechanism, and therapeutic effective components of QHSSD on both OA and RA. A total of 153 active ingredients in QHSSD were identified, 142 of which associated with 59 potential targets for the two diseases were identified. By constructing the protein-protein interaction network and the compound-target-disease network, 72 compounds and 10 proteins were obtained as the hub targets of QHSSD against OA and RA. The hub genes of ESR1, PTGS2, PPARG, IL1B, TNF, MMP2, IL6, CYP3A4, MAPK8, and ALB were mainly involved in osteoclast differentiation, the NF-κB and TNF signaling pathways. Moreover, molecular docking results showed that the screened active compounds had a high affinity for the hub genes. This study provides new insight into the molecular mechanisms behind how QHSSD presents homotherapy-for-heteropathy therapeutic efficacy in both OA and RA. For the first time, a two-disease model was linked with a TCM formula using network pharmacology to identify the key active components and understand the common mechanisms of its multi-pathway regulation. This study will inspire more innovative and important studies on the modern research of TCM formulas.
MeSH term(s)	Arthritis, Rheumatoid/drug therapy ; Arthritis, Rheumatoid/genetics ; Cell Differentiation/drug effects ; Databases, Pharmaceutical/statistics & numerical data ; Drugs, Chinese Herbal/metabolism ; Drugs, Chinese Herbal/pharmacology ; Gene Expression/drug effects ; Humans ; Molecular Docking Simulation ; Osteoarthritis/drug therapy ; Osteoarthritis/genetics ; Osteoclasts/cytology ; Pharmacology/methods ; Protein Interaction Maps
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2020-10-01
Publishing country	England
Document type	Journal Article
ISSN	1476-928X
ISSN (online)	1476-928X
DOI	10.1016/j.compbiolchem.2020.107397
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Article ; Online: Qiang-Gan formula extract improves non-alcoholic steatohepatitis via regulating bile acid metabolism and gut microbiota in mice.

Li, Qiong / Li, Meng / Li, Fenghua / Zhou, Wenjun / Dang, Yanqi / Zhang, Li / Ji, Guang

Journal of ethnopharmacology

2020 Volume 258, Page(s) 112896

Abstract: Ethnopharmacological relevance: Qiang-Gan formula is a traditional Chinese medicine formula ... the effect of Qiang-Gan formula extract (QGE) on non-alcoholic steatohepatitis (NASH) and its underlying ...

Abstract	Ethnopharmacological relevance: Qiang-Gan formula is a traditional Chinese medicine formula, which has been widely used in treating liver diseases in China. Aim of the study: To investigate the effect of Qiang-Gan formula extract (QGE) on non-alcoholic steatohepatitis (NASH) and its underlying possible mechanisms. Materials and methods: The high-performance liquid chromatography finger-print method was used for the quality control of chemical components in QGE. Methionine- and choline-deficient diet-induced NASH mice were administrated with QGE via gavage for four weeks. Phenotypic parameters including liver histological change as well as serum levels of alanine transaminase (ALT), aspartate transaminase (AST) were detected. Bile acid profile in the serum, liver and fecal samples was analyzed by gas chromatography-mass spectrometer technique, and fecal microbiota was detected by 16S rDNA sequencing. Expression of liver G protein-coupled bile acid receptor 1 (TGR5), farnesiod X receptor (FXR), tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) as well as molecules in nuclear factor kappa B (NF-κB) pathway was assayed by immunohistochemistry staining, RT-qPCR, or Western blot, respectively. Results: QGE alleviated liver inflammation, reduced serum ALT and AST levels and liver TNF-α and IL-1β expression in NASH mice. It also decreased liver and serum BA concentration and increased fecal lithocholicacid (LCA) production in this animal model. QGE altered the structure of gut microbiota, predominantly increased LCA-producing bacteria Bacteroides and Clostridium in NASH mice. In addition, the expression of liver TGR5 but not FXR was increased, and the molecules in NF-κB pathway were decreased in QGE-treated NASH mice. Conclusions: QGE was effective in preventing NASH, possibly by regulation of gut microbiota-mediated LCA production, promotion of TGR5 expression and suppression of the NF-κB activation.
MeSH term(s)	Animals ; Bile Acids and Salts/metabolism ; Disease Models, Animal ; Drugs, Chinese Herbal/pharmacology ; Gastrointestinal Microbiome/drug effects ; Lithocholic Acid/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; NF-kappa B/metabolism ; Non-alcoholic Fatty Liver Disease/drug therapy ; Non-alcoholic Fatty Liver Disease/physiopathology ; Receptors, G-Protein-Coupled/genetics
Chemical Substances	Bile Acids and Salts ; Drugs, Chinese Herbal ; Gpbar1 protein, mouse ; NF-kappa B ; Receptors, G-Protein-Coupled ; qianggan ; Lithocholic Acid (5QU0I8393U)
Language	English
Publishing date	2020-04-20
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.112896
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Zs.A 1494: Show issues

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Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Book: Kou qiang yi xue shi

Zhou, Xuedong

2013

Author's details	zhu bian Zhou Xuedong, Tang Jie, Tan Jing
MeSH term(s)	History of Dentistry
Language	Chinese
Size	13, 414 pages :, illustrations, portraits
Edition	Di 1 ban.
Document type	Book
ISBN	9787117171892 ; 7117171898
Database	Catalogue of the US National Library of Medicine (NLM)

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Article ; Online: Ancient DNA reveals genetic connections between early Di-Qiang and Han Chinese.

Li, Jiawei / Zeng, Wen / Zhang, Ye / Ko, Albert Min-Shan / Li, Chunxiang / Zhu, Hong / Fu, Qiaomei / Zhou, Hui

BMC evolutionary biology

2017 Volume 17, Issue 1, Page(s) 239

Abstract: Background: Ancient Di-Qiang people once resided in the Ganqing region of China, adjacent ... to the Central Plain area from where Han Chinese originated. While gene flow between the Di-Qiang and Han Chinese ... from an early Di-Qiang site (Mogou site dated ~4000 years old) and compared them to other ancient DNA across ...

Abstract	Background: Ancient Di-Qiang people once resided in the Ganqing region of China, adjacent to the Central Plain area from where Han Chinese originated. While gene flow between the Di-Qiang and Han Chinese has been proposed, there is no evidence to support this view. Here we analyzed the human remains from an early Di-Qiang site (Mogou site dated ~4000 years old) and compared them to other ancient DNA across China, including an early Han-related site (Hengbei site dated ~3000 years old) to establish the underlying genetic relationship between the Di-Qiang and ancestors of Han Chinese. Results: We found Mogou mtDNA haplogroups were highly diverse, comprising 14 haplogroups: A, B, C, D (D, D4, D5), F, G, M7, M8, M10, M13, M25, N, N9a, and Z. In contrast, Mogou males were all Y-DNA haplogroup O3a2/P201; specifically one male was further assigned to O3a2c1a/M117 using targeted unique regions on the non-recombining region of the Y-chromosome. We compared Mogou to 7 other ancient and 38 modern Chinese groups, in a total of 1793 individuals, and found that Mogou shared close genetic distances with Taojiazhai (a more recent Di-Qiang population), Hengbei, and Northern Han. We modeled their interactions using Approximate Bayesian Computation, and support was given to a potential admixture of ~13-18% between the Mogou and Northern Han around 3300-3800 years ago. Conclusions: Mogou harbors the earliest genetically identifiable Di-Qiang, ancestral to the Taojiazhai, and up to ~33% paternal and ~70% of its maternal haplogroups could be found in present-day Northern Han Chinese.
MeSH term(s)	Asian Continental Ancestry Group/genetics ; Bayes Theorem ; China ; Chromosomes, Human, Y/genetics ; Computer Simulation ; DNA, Ancient ; DNA, Mitochondrial/genetics ; Ethnic Groups/genetics ; Genetics, Population ; Geography ; Haplotypes/genetics ; Humans ; Male ; Models, Genetic ; Phylogeny ; Polymorphism, Single Nucleotide/genetics ; Principal Component Analysis ; Probability ; Time Factors
Chemical Substances	DNA, Ancient ; DNA, Mitochondrial
Language	English
Publishing date	2017-12-04
Publishing country	England
Document type	Journal Article
ISSN	1471-2148
ISSN (online)	1471-2148
DOI	10.1186/s12862-017-1082-0
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Book: Zhongguo kou qiang yi xue jiao yu shi

Zhou, Xuedong

2015

Author's details	Zhou Xuedong, Ye Ling zhu bian
MeSH term(s)	Education, Dental/history ; Schools, Dental/history
Keywords	China
Language	Chinese
Size	vi, 321 p. :, ill., ports
Edition	Di 1 ban.
Publisher	Gao deng jiao yu chu ban she
Publishing place	Beijing
Document type	Book
ISBN	9787040420104 ; 7040420104
Database	Catalogue of the US National Library of Medicine (NLM)

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