LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 54

Search options

  1. Book: The Renin-Angiotensin-Aldosterone System

    Thatcher, Sean E.

    methods and protocols

    (Methods in molecular biology ; 1614 ; Springer protocols)

    2017  

    Author's details edited by Sean E. Thatcher
    Series title Methods in molecular biology ; 1614
    Springer protocols
    Collection
    Keywords fluorescent substrate ; radio telemetry ; mass spectrometry ; angiotensin peptides ; RAAS components ; mammalian disease ; ACE2 activity
    Subject code 570
    Language English
    Size x, 201 Seiten, Illustrationen, 25.4 cm x 17.8 cm
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT019321095
    ISBN 978-1-4939-7028-5 ; 1-4939-7028-3 ; 9781493970308 ; 1493970305
    Database Catalogue ZB MED Medicine, Health

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1614- verfügbar in Königswinter Königswinter

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques.

    Thatcher, Sean E

    Methods in molecular biology (Clifton, N.J.)

    2017  Volume 1614, Page(s) 1–19

    Abstract: The renin-angiotensin-aldosterone system (RAAS) is a complex system of enzymes, receptors, and peptides that help to control blood pressure and fluid homeostasis. Techniques in studying the RAAS can be difficult due to such factors as peptide/enzyme ... ...

    Abstract The renin-angiotensin-aldosterone system (RAAS) is a complex system of enzymes, receptors, and peptides that help to control blood pressure and fluid homeostasis. Techniques in studying the RAAS can be difficult due to such factors as peptide/enzyme stability and receptor localization. This paper gives a brief account of the different components of the RAAS and current methods in measuring each component. There is also a discussion of different methods in measuring stem and immune cells by flow cytometry, hypertension, atherosclerosis, oxidative stress, energy balance, and other RAAS-activated phenotypes. While studies on the RAAS have been performed for over 100 years, new techniques have allowed scientists to come up with new insights into this system. These techniques are detailed in this Methods in Molecular Biology Series and give students new to studying the RAAS the proper controls and technical details needed to perform each procedure.
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7030-8_1
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: TGF-β Signaling: New Insights Into Aortic Aneurysms.

    Thatcher, Sean E

    EBioMedicine

    2016  Volume 12, Page(s) 24–25

    MeSH term(s) Animals ; Aortic Aneurysm/genetics ; Aortic Aneurysm/metabolism ; Humans ; Mutation ; Signal Transduction ; Smad3 Protein/deficiency ; Smad3 Protein/genetics ; Smad3 Protein/metabolism ; Transforming Growth Factor beta/metabolism
    Chemical Substances Smad3 Protein ; Transforming Growth Factor beta
    Language English
    Publishing date 2016-10
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 2851331-9
    ISSN 2352-3964
    ISSN (online) 2352-3964
    DOI 10.1016/j.ebiom.2016.09.026
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 7090: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Commentary for Clancy, P et al., ARBs and ERK activation: new insights on human atherosclerosis.

    Thatcher, Sean

    Atherosclerosis

    2014  Volume 236, Issue 1, Page(s) 131–132

    MeSH term(s) 1-Sarcosine-8-Isoleucine Angiotensin II/pharmacology ; Angiotensin II Type 1 Receptor Blockers/pharmacology ; Biphenyl Compounds/pharmacology ; Cytokines/secretion ; Endothelium, Vascular/drug effects ; Female ; Humans ; Male ; Mitogen-Activated Protein Kinase 1/metabolism ; Mitogen-Activated Protein Kinase 3/metabolism ; Peptides/pharmacology ; Renin-Angiotensin System/drug effects ; Tetrazoles/pharmacology
    Chemical Substances Angiotensin II Type 1 Receptor Blockers ; Biphenyl Compounds ; Cytokines ; DX600 peptide ; Peptides ; Tetrazoles ; 1-Sarcosine-8-Isoleucine Angiotensin II (9088-01-1) ; MAPK1 protein, human (EC 2.7.11.24) ; Mitogen-Activated Protein Kinase 1 (EC 2.7.11.24) ; Mitogen-Activated Protein Kinase 3 (EC 2.7.11.24) ; irbesartan (J0E2756Z7N)
    Language English
    Publishing date 2014-09
    Publishing country Ireland
    Document type Journal Article ; Comment
    ZDB-ID 80061-2
    ISSN 1879-1484 ; 0021-9150
    ISSN (online) 1879-1484
    ISSN 0021-9150
    DOI 10.1016/j.atherosclerosis.2014.06.026
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 226: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: TGF-β Signaling

    Sean E. Thatcher

    EBioMedicine, Vol 12, Iss C, Pp 24-

    New Insights Into Aortic Aneurysms

    2016  Volume 25

    Keywords Medicine ; R ; Medicine (General) ; R5-920
    Language English
    Publishing date 2016-10-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: Blood Pressure Monitoring Using Radio Telemetry Method in Mice.

    Wang, Yu / Thatcher, Sean E / Cassis, Lisa A

    Methods in molecular biology (Clifton, N.J.)

    2017  Volume 1614, Page(s) 75–85

    Abstract: The TA11PA-C10 implantable transmitter (Data Sciences International, DSI) is designed to measure blood pressure (BP) and activity in freely moving laboratory mice. The fluid filled catheter is placed in the free flowing blood of the systemic artery ( ... ...

    Abstract The TA11PA-C10 implantable transmitter (Data Sciences International, DSI) is designed to measure blood pressure (BP) and activity in freely moving laboratory mice. The fluid filled catheter is placed in the free flowing blood of the systemic artery (inserted into the left carotid artery and extended into the aorta), and the transmitter body is placed in a benign location for long-term biocompatibility. The transmitter can be used to monitor BP in mice (as small as 17 g) under normal physiological and unrestricted conditions 24 h a day while remaining free from stress associated with human interaction. Thus, telemetry is considered the gold standard for BP monitoring in small animals such as mice. However, this methodology does require a good understanding of the system as well as appropriate training to perform the delicate transmitter implantation surgery.
    MeSH term(s) Animals ; Blood Pressure/physiology ; Carotid Arteries/surgery ; Catheter Ablation/instrumentation ; Catheter Ablation/methods ; Catheter Ablation/veterinary ; Mice ; Software ; Telemetry/methods ; Telemetry/veterinary
    Language English
    Publishing date 2017-05-11
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7030-8_7
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article ; Online: Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta.

    Wang, Yu / Cassis, Lisa A / Thatcher, Sean E

    Methods in molecular biology (Clifton, N.J.)

    2017  Volume 1614, Page(s) 61–67

    Abstract: The use of fluorogenic substrates to measure enzymatic activity is widely used to understand function within different experimental models. ACE2 is important in understanding the balance between AngII and Ang-(1-7) and how this balance could then in turn ...

    Abstract The use of fluorogenic substrates to measure enzymatic activity is widely used to understand function within different experimental models. ACE2 is important in understanding the balance between AngII and Ang-(1-7) and how this balance could then in turn influence hypertension or other disease outcomes. Here, we describe a method to measure ACE2 activity in abdominal aorta of hyperlipidemic mice under both saline and AngII infusion.
    MeSH term(s) Angiotensin II/chemistry ; Angiotensin II/metabolism ; Animals ; Aorta, Abdominal/drug effects ; Aorta, Abdominal/enzymology ; Disease Models, Animal ; Fluorescent Antibody Technique/methods ; Fluorescent Dyes/metabolism ; Hyperlipidemias/enzymology ; Hyperlipidemias/pathology ; Mice ; Mice, Knockout ; Peptidyl-Dipeptidase A/analysis ; Peptidyl-Dipeptidase A/metabolism ; Receptors, LDL/physiology ; Sodium Chloride/administration & dosage ; Vasoconstrictor Agents/metabolism
    Chemical Substances Fluorescent Dyes ; Receptors, LDL ; Vasoconstrictor Agents ; Angiotensin II (11128-99-7) ; Sodium Chloride (451W47IQ8X) ; Peptidyl-Dipeptidase A (EC 3.4.15.1) ; angiotensin converting enzyme 2 (EC 3.4.17.-)
    Keywords covid19
    Language English
    Publishing date 2017-05-03
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7030-8_5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: Measuring Blood Pressure Using a Noninvasive Tail Cuff Method in Mice.

    Wang, Yu / Thatcher, Sean E / Cassis, Lisa A

    Methods in molecular biology (Clifton, N.J.)

    2017  Volume 1614, Page(s) 69–73

    Abstract: The renin angiotensin system (RAS) is well known for its role in regulating blood pressure (BP). An activated RAS contributes to elevated blood pressure and is evident in both human and animal models of hypertension. Drugs that target the classic ... ...

    Abstract The renin angiotensin system (RAS) is well known for its role in regulating blood pressure (BP). An activated RAS contributes to elevated blood pressure and is evident in both human and animal models of hypertension. Drugs that target the classic vasoconstrictive arm of the RAS (angiotensin II/AT1 receptor signaling) are potent anti-hypertensive agents in clinical setting. However, the newly discovered angiotensin-converting enzyme 2 (ACE2)/angiotensin-(1-7)/Mas receptor axis added new vitality to the hypertension field. Advances in genetic manipulation and the relative low cost made the mouse model as one of the most popular animal models to study hypertension. Since a reliable and accurate method for BP assessment is the key for such experiments, here we provide a protocol for BP measurement in mice using a noninvasive BP system. The CODA noninvasive BP system (a tail-cuff Method, Kent Scientific Corporation) enables blood pressure (BP) measurements in mice. This method uses a specialized volume pressure recording (VPR) sensor, and measures blood volume changes that are placed over the animal's tail. Mice do need to be restrained in specific holders and artificially heated to maintain normal BP.
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7030-8_6
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Therapeutic Assessment of Combination Therapy with a Neprilysin Inhibitor and Angiotensin Type 1 Receptor Antagonist on Angiotensin II-Induced Atherosclerosis, Abdominal Aortic Aneurysms, and Hypertension.

    AlSiraj, Yasir / Thatcher, Sean E / Liang, Ching Ling / Ali, Heba / Ensor, Mark / Cassis, Lisa A

    The Journal of pharmacology and experimental therapeutics

    2021  Volume 377, Issue 3, Page(s) 326–335

    Abstract: Combined neprilysin (NEP) inhibition (sacubitril) and angiotensin type 1 receptor (AT1R) antagonism (valsartan) is used in the treatment of congestive heart failure and is gaining interest for other angiotensin II (AngII)-related cardiovascular diseases. ...

    Abstract Combined neprilysin (NEP) inhibition (sacubitril) and angiotensin type 1 receptor (AT1R) antagonism (valsartan) is used in the treatment of congestive heart failure and is gaining interest for other angiotensin II (AngII)-related cardiovascular diseases. In addition to heart failure, AngII promotes hypertension, atherosclerosis, and abdominal aortic aneurysms (AAAs). Similarly, NEP substrates or products have broad effects on the cardiovascular system. In this study, we examined NEP inhibition (with sacubitril) and AT1R antagonism (with valsartan) alone or in combination on AngII-induced hypertension, atherosclerosis, or AAAs in male low-density lipoprotein receptor-deficient mice. Preliminary studies assessed drug delivery via osmotic minipumps for simultaneous release of sacubitril and/or valsartan with AngII over 28 days. Mice were infused with AngII (1000 ng/kg per minute) in the absence (vehicle) or presence of sacubitril (1, 6, or 9 mg/kg per day), valsartan (0.3, 0.5, 1, 6, or 20 mg/kg per day), or the combination thereof (1 and 0.3, or 9 or 0.5 mg/kg per day of sacubitril and valsartan, respectively). Plasma AngII and renin concentrations increased 4-fold at higher valsartan doses, indicative of removal of AngII negative feedback on renin. Sacubitril doubled plasma AngII concentrations at lower doses (1 mg/kg per day). Valsartan dose-dependently decreased systolic blood pressure, aortic atherosclerosis, and AAAs of AngII-infused mice, whereas sacubitril had no effect on atherosclerosis or AAAs but reduced blood pressure of AngII-infused mice. Combination therapy with sacubitril and valsartan did not provide additive benefits. These results suggest limited effects of combination therapy with NEP inhibition and AT1R antagonism against AngII-induced hypertension, atherosclerosis, or AAAs. SIGNIFICANCE STATEMENT: The combination of valsartan (angiotensin type 1 receptor antagonist) and sacubitril (neprilysin inhibitor) did not provide benefit above valsartan alone on AngII-induced hypertension, atherosclerosis, or abdominal aortic aneurysms in low-density lipoprotein receptor-deficient male mice. These results do not support this drug combination in therapy of these AngII-induced cardiovascular diseases.
    MeSH term(s) Aminobutyrates ; Angiotensin II ; Antihypertensive Agents ; Atherosclerosis ; Biphenyl Compounds ; Neprilysin ; Animals ; Mice
    Chemical Substances Aminobutyrates ; Angiotensin II (11128-99-7) ; Antihypertensive Agents ; Biphenyl Compounds ; Neprilysin (EC 3.4.24.11) ; sacubitril (17ERJ0MKGI)
    Language English
    Publishing date 2021-03-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 3106-9
    ISSN 1521-0103 ; 0022-3565
    ISSN (online) 1521-0103
    ISSN 0022-3565
    DOI 10.1124/jpet.121.000525
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.40: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Adipocyte-Derived Serum Amyloid A Promotes Angiotensin II-Induced Abdominal Aortic Aneurysms in Obese C57BL/6J Mice.

    Shridas, Preetha / Ji, Ailing / Trumbauer, Andrea C / Noffsinger, Victoria P / Leung, Steve W / Dugan, Adam J / Thatcher, Sean E / Cassis, Lisa A / de Beer, Frederick C / Webb, Nancy R / Tannock, Lisa R

    Arteriosclerosis, thrombosis, and vascular biology

    2022  Volume 42, Issue 5, Page(s) 632–643

    Abstract: Background: Obesity increases the risk for human abdominal aortic aneurysms (AAAs) and enhances Ang II (angiotensin II)-induced AAA formation in C57BL/6J mice. Obesity is also associated with increases in perivascular fat that expresses proinflammatory ... ...

    Abstract Background: Obesity increases the risk for human abdominal aortic aneurysms (AAAs) and enhances Ang II (angiotensin II)-induced AAA formation in C57BL/6J mice. Obesity is also associated with increases in perivascular fat that expresses proinflammatory markers including SAA (serum amyloid A). We previously reported that deficiency of SAA significantly reduces Ang II-induced inflammation and AAA in hyperlipidemic apoE-deficient mice. In this study. we investigated whether adipose tissue-derived SAA plays a role in Ang II-induced AAA in obese C57BL/6J mice.
    Methods: The development of AAA was compared between male C57BL/6J mice (wild type), C57BL/6J mice lacking SAA1.1, SAA2.1, and SAA3 (TKO); and TKO mice harboring a doxycycline-inducible, adipocyte-specific SAA1.1 transgene (TKO-Tg
    Results: In response to Ang II infusion, SAA expression was significantly increased in perivascular fat of obese C57BL/6J mice. Maximal luminal diameters of the abdominal aorta were determined by ultrasound before and after Ang II infusion, which indicated a significant increase in aortic luminal diameters in wild type and TKO-TG
    Conclusions: We demonstrate for the first time that SAA deficiency protects obese C57BL/6J mice from Ang II-induced AAA. SAA expression only in adipocytes is sufficient to cause AAA in obese mice infused with Ang II.
    MeSH term(s) Adipocytes/metabolism ; Angiotensin II/pharmacology ; Animals ; Aortic Aneurysm, Abdominal/chemically induced ; Aortic Aneurysm, Abdominal/genetics ; Apolipoproteins E/genetics ; Disease Models, Animal ; Doxycycline/adverse effects ; Male ; Matrix Metalloproteinases ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Obese ; Obesity/complications ; Serum Amyloid A Protein/genetics ; Serum Amyloid A Protein/metabolism
    Chemical Substances Apolipoproteins E ; Serum Amyloid A Protein ; Angiotensin II (11128-99-7) ; Matrix Metalloproteinases (EC 3.4.24.-) ; Doxycycline (N12000U13O)
    Language English
    Publishing date 2022-03-28
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 1221433-4
    ISSN 1524-4636 ; 1079-5642
    ISSN (online) 1524-4636
    ISSN 1079-5642
    DOI 10.1161/ATVBAHA.121.317225
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1705: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top