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Article ; Online: Novel ultrasound assisted extraction and d-SPE clean-up for the analysis of multiple legacy and emerging organic contaminants in edible fish.

Santini, Saul / Baini, Matteo / Martellini, Tania / Bissoli, Matteo / Galli, Matteo / Concato, Margherita / Fossi, Maria Cristina / Cincinelli, Alessandra

Food chemistry

2024 Volume 443, Page(s) 138582

Abstract: ... Employing ultrasound extraction coupled with dispersive solid-phase extraction (d-SPE) as a cleanup ...

Abstract	Polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), novel brominated flame retardants (NBFRs), phthalate esters (PAEs) are pervasive environmental pollutants, posing threats to both ecosystems and human health. Although several analytical methods were developed for these compounds, they are not performed simultaneously. This study addresses the need for a sustainable, novel, analytical approach capable of simultaneously determining these diverse chemical classes in edible fish muscles. Employing ultrasound extraction coupled with dispersive solid-phase extraction (d-SPE) as a cleanup procedure, the method was compared to conventional techniques, revealing significant improvements. Analytical parameters were thoroughly assessed, and the innovative method demonstrated notable advantages, reducing extraction and purification times by approximately 74-80 % and solvent consumption by around 94-97 %. Applied to Mediterranean Sea fish samples, the results underscore the method's potential as a viable, sustainable alternative to traditional approaches, promising enhanced efficiency and reduced environmental impact.
MeSH term(s)	Humans ; Animals ; Gas Chromatography-Mass Spectrometry/methods ; Ecosystem ; Polychlorinated Biphenyls/analysis ; Environmental Pollutants/analysis ; Solid Phase Extraction/methods ; Halogenated Diphenyl Ethers/analysis ; Flame Retardants/analysis ; Environmental Monitoring
Chemical Substances	Polychlorinated Biphenyls (DFC2HB4I0K) ; Environmental Pollutants ; Halogenated Diphenyl Ethers ; Flame Retardants
Language	English
Publishing date	2024-01-29
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2024.138582
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Article ; Online: Analysis of HIV quasispecies and virological outcome of an HIV D+/R+ kidney-liver transplantation.

Rozera, Gabriella / Visco-Comandini, Ubaldo / Giombini, Emanuela / Santini, Francesco / Forbici, Federica / Berno, Giulia / Gruber, Cesare / De Paolis, Paolo / Colonnelli, Roberto / D'Offizi, Gianpiero / Ettorre, Giuseppe Maria / Grossi, Paolo / Capobianchi, Maria Rosaria / Ippolito, Giuseppe / Abbate, Isabella

Virology journal

2022 Volume 19, Issue 1, Page(s) 4

Abstract: ... intervention. This study involves an HIV D+/R+ kidney-liver transplantation, where PBMC-associated HIV ...

Abstract	Introduction: Transplantation among HIV positive patients may be a valuable therapeutic intervention. This study involves an HIV D+/R+ kidney-liver transplantation, where PBMC-associated HIV quasispecies were analyzed in donor and transplant recipients (TR) prior to transplantation and thereafter, together with standard viral monitoring. Methods: The donor was a 54 year of age HIV infected woman: kidney and liver recipients were two HIV infected men, aged 49 and 61. HIV quasispecies in PBMC was analyzed by ultra-deep sequencing of V3 env region. During TR follow-up, plasma HIV-1 RNA, HIV-1 DNA in PBMC, analysis of proviral integration sites and drug-resistance genotyping were performed. Other virological and immunological monitoring included CMV and EBV DNA quantification in blood and CD4 T cell counts. Results: Donor and TR were all ART-HIV suppressed at transplantation. Thereafter, TR maintained a nearly suppressed HIV-1 viremia, but HIV-1 RNA blips and the increase of proviral integration sites in PBMC attested some residual HIV replication. A transient peak in HIV-1 DNA occurred in the liver recipient. No major changes of drug-resistance genotype were detected after transplantation. CMV and EBV transient reactivations were observed only in the kidney recipient, but did not require specific treatment. CD4 counts remained stable. No intermixed quasispecies between donor and TR was observed at transplantation or thereafter. Despite signs of viral evolution in TR, HIV genetic heterogeneity did not increase over the course of the months of follow up. Conclusions: No evidence of HIV superinfection was observed in the donor nor in the recipients. The immunosuppressive treatment administrated to TR did not result in clinical relevant viral reactivations.
MeSH term(s)	HIV Infections ; Humans ; Kidney ; Leukocytes, Mononuclear ; Liver ; Liver Transplantation ; Quasispecies
Language	English
Publishing date	2022-01-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2160640-7
ISSN	1743-422X ; 1743-422X
ISSN (online)	1743-422X
ISSN	1743-422X
DOI	10.1186/s12985-021-01730-w
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Article ; Online: Degradation kinetic (D

Manzo, Nadia / Santini, Antonello / Pizzolongo, Fabiana / Aiello, Alessandra / Romano, Raffaele

Natural product research

2018 Volume 33, Issue 13, Page(s) 1835–1841

Abstract: ... antioxidant effects. No information about decimal reduction time (D ...

Abstract	Heat treatments can cause degradation in tomatoes of lycopene which has important antioxidant effects. No information about decimal reduction time (D
MeSH term(s)	Antioxidants ; Carotenoids/analysis ; Food Contamination/prevention & control ; Food Handling/methods ; Food Handling/standards ; Food Microbiology ; Hot Temperature ; Kinetics ; Lycopene/chemistry ; Lycopersicon esculentum/chemistry ; Lycopersicon esculentum/metabolism ; Lycopersicon esculentum/microbiology ; Pasteurization
Chemical Substances	Antioxidants ; Carotenoids (36-88-4) ; Lycopene (SB0N2N0WV6)
Language	English
Publishing date	2018-05-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2185747-7
ISSN	1478-6427 ; 1478-6419
ISSN (online)	1478-6427
ISSN	1478-6419
DOI	10.1080/14786419.2018.1477147
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Article ; Online: Correction: Vitamin D status and supplementation before and after Bariatric Surgery: Recommendations based on a systematic review and meta‑analysis.

Giustina, Andrea / di Filippo, Luigi / Facciorusso, Antonio / Adler, Robert A / Binkley, Neil / Bollerslev, Jens / Bouillon, Roger / Casanueva, Felipe F / Cavestro, Giulia Martina / Chakhtoura, Marlene / Conte, Caterina / Donini, Lorenzo M / Ebeling, Peter R / Fassio, Angelo / Frara, Stefano / Gagnon, Claudia / Latella, Giovanni / Marcocci, Claudio / Mechanick, Jeffrey I /

Minisola, Salvatore / Rizzoli, René / Santini, Ferruccio / Shaker, Joseph L / Sempos, Christopher / Ulivieri, Fabio Massimo / Virtanen, Jyrki K / Napoli, Nicola / Schafer, Anne L / Bilezikian, John P

Reviews in endocrine & metabolic disorders

2024 Volume 25, Issue 2, Page(s) 447–448

Language	English
Publishing date	2024-03-19
Publishing country	Germany
Document type	Published Erratum
ZDB-ID	2185718-0
ISSN	1573-2606 ; 1389-9155
ISSN (online)	1573-2606
ISSN	1389-9155
DOI	10.1007/s11154-023-09837-x
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Article ; Online: Adaptation transculturelle en Français du questionnaire évaluant la qualité de vie des patients opérés d’un cancer de la thyroïde : THYCA-QoL.

Scheller, Boris / Santini, Joseph / Anota, Amélie / Poissonnet, Gilles / Chateau, Y / Schiappa, Renaud / Benisvy, Danielle / Dassonville, Olivier / Bozec, Alexandre / Chamorey, Emmanuel

Bulletin du cancer

2021 Volume 108, Issue 7-8, Page(s) 696–704

Abstract: Introduction: The aim of this study was to translate into French the 24 items of the THYCA-QoL questionnaire used in thyroid cancers and then to study its psychometric properties.: Materials and methods: The THYCA-QoL is a specific questionnaire for ... ...

Title translation	Cross-cultural adaptation of the French version of the thyroid cancer-specific quality of life questionnaire: THYCA-QoL.
Abstract	Introduction: The aim of this study was to translate into French the 24 items of the THYCA-QoL questionnaire used in thyroid cancers and then to study its psychometric properties. Materials and methods: The THYCA-QoL is a specific questionnaire for evaluating the quality of life of patients undergoing thyroid cancer surgery. It consists of 24 items and is divided into seven dimensions and six isolated questions. The translation has been carried out according to the recommendations of the EORTC. Validation of the translated version was obtained by finding a consensus of experts for each of the items. Results: All the original questions of the questionnaire have been adapted into French. The translated questionnaire, named THYCA-CoL-fr, was tested on 60 patients (65 % female), mean age 54.5 years. All questions were well accepted and understood and no missing data were reported. Eight patients (13 %) proposed an item correction to the questionnaire. No attenuation effects (floor or ceiling) were detected. The internal structure was comparable to the original questionnaire: Cronbach α coefficients varied from 0.53 for the oropharyngeal dimension to 0.88 for the voice dimension. The scree-plot highlighted the seven dimensions of the English version. Conclusion: THYCA-QoL-fr is the first specific French language questionnaire to evaluate the quality of life in thyroid cancer patients undergoing surgery. These first exploratory psychometric results confirmed the conceptual similarity of the French translation and the English version.
MeSH term(s)	Cross-Cultural Comparison ; Female ; Health Surveys/standards ; Humans ; Language ; Male ; Middle Aged ; Psychometrics ; Quality of Life ; Thyroid Neoplasms/surgery ; Translations
Language	French
Publishing date	2021-04-22
Publishing country	France
Document type	Journal Article ; Validation Study
ZDB-ID	213270-9
ISSN	1769-6917 ; 0007-4551
ISSN (online)	1769-6917
ISSN	0007-4551
DOI	10.1016/j.bulcan.2021.01.009
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Article ; Online: Systematic vitamin D supplementation is associated with improved outcomes and reduced thyroid adverse events in patients with cancer treated with immune checkpoint inhibitors: results from the prospective PROVIDENCE study.

Bersanelli, Melissa / Cortellini, Alessio / Leonetti, Alessandro / Parisi, Alessandro / Tiseo, Marcello / Bordi, Paola / Michiara, Maria / Bui, Simona / Cosenza, Agnese / Ferri, Leonarda / Giudice, Giulia Claire / Testi, Irene / Rapacchi, Elena / Camisa, Roberta / Vincenzi, Bruno / Caruso, Giuseppe / Rauti, Antonio Natale / Arturi, Federica / Tucci, Marco /

Santo, Valentina / Ricozzi, Valentina / Burtet, Vanessa / Sgargi, Paolo / Todeschini, Renata / Zustovich, Fable / Stucci, Luigia Stefania / Santini, Daniele / Buti, Sebastiano

Cancer immunology, immunotherapy : CII

2023 Volume 72, Issue 11, Page(s) 3707–3716

Abstract: Background: Hypovitaminosis D can have a negative prognostic impact in patients with cancer ... Vitamin D has a demonstrated role in T-cell-mediated immune activation. We hypothesized that systematic ... vitamin D repletion could impact clinical outcomes in patients with cancer receiving immune-checkpoint ...

Abstract	Background: Hypovitaminosis D can have a negative prognostic impact in patients with cancer. Vitamin D has a demonstrated role in T-cell-mediated immune activation. We hypothesized that systematic vitamin D repletion could impact clinical outcomes in patients with cancer receiving immune-checkpoint inhibitors (ICIs). Methods: We planned a prospective observational study (PROVIDENCE) to assess serum vitamin D levels in patients with advanced cancer receiving ICIs (cohort 1 at treatment initiation, cohort 2 during treatment) and the impact of systematic repletion on survival and toxicity outcomes. In an exploratory analysis, we compared the clinical outcomes of cohort 1 with a control cohort of patients followed at the participating centers who did not receive systematic vitamin D repletion. Results: Overall, 164 patients were prospectively recruited in the PROVIDENCE study. In cohort 1, consisting of 101 patients with 94.1% hypovitaminosis (≤ 30 ng/ml) at baseline, adequate repletion with cholecalciferol was obtained in 70.1% at the three months re-assessment. Cohort 2 consisted of 63 patients assessed for vitamin D at a median time of 3.7 months since immunotherapy initiation, with no patients having adequate levels (> 30 ng/ml). Even in cohort 2, systematic supplementation led to adequate levels in 77.8% of patients at the three months re-assessment. Compared to a retrospective control group of 238 patients without systematic vitamin D repletion, PROVIDENCE cohort 1 showed longer overall survival (OS, p = 0.013), time to treatment failure (TTF, p = 0.017), and higher disease control rate (DCR, p = 0.016). The Inverse Probability of Treatment Weighing (IPTW) fitted multivariable Cox regression confirmed the significantly decreased risk of death (HR 0.55, 95%CI: 0.34-0.90) and treatment discontinuation (HR 0.61, 95%CI: 0.40-0.91) for patients from PROVIDENCE cohort 1 in comparison to the control cohort. In the context of longer treatment exposure, the cumulative incidence of any grade immune-related adverse events (irAEs) was higher in the PROVIDENCE cohort 1 compared to the control cohort. Nevertheless, patients from cohort 1 experienced a significantly decreased risk of all grade thyroid irAEs than the control cohort (OR 0.16, 95%CI: 0.03-0.85). Conclusion: The PROVIDENCE study suggests the potential positive impact of early systematic vitamin D supplementation on outcomes of patients with advanced cancer receiving ICIs and support adequate repletion as a possible prophylaxis for thyroid irAEs.
MeSH term(s)	Humans ; Immune Checkpoint Inhibitors/adverse effects ; Vitamin D/therapeutic use ; Retrospective Studies ; Prospective Studies ; Thyroid Gland ; Antineoplastic Agents, Immunological/therapeutic use ; Neoplasms/drug therapy ; Dietary Supplements
Chemical Substances	Immune Checkpoint Inhibitors ; Vitamin D (1406-16-2) ; Antineoplastic Agents, Immunological
Language	English
Publishing date	2023-08-28
Publishing country	Germany
Document type	Observational Study ; Journal Article
ZDB-ID	195342-4
ISSN	1432-0851 ; 0340-7004
ISSN (online)	1432-0851
ISSN	0340-7004
DOI	10.1007/s00262-023-03522-3
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Article ; Online: Complement Factor D (adipsin) Levels Are Elevated in Acquired Partial Lipodystrophy (Barraquer-Simons syndrome).

Corvillo, Fernando / González-Sánchez, Laura / López-Lera, Alberto / Arjona, Emilia / Ceccarini, Giovanni / Santini, Ferruccio / Araújo-Vilar, David / Brown, Rebecca J / Villarroya, Joan / Villarroya, Francesc / Rodríguez de Córdoba, Santiago / Caballero, Teresa / Nozal, Pilar / López-Trascasa, Margarita

International journal of molecular sciences

2021 Volume 22, Issue 12

Abstract: ... a rare form of acquired partial lipodystrophy. Complement Factor D (FD) is a serine protease ...

Abstract	Complement overactivation has been reported in most patients with Barraquer-Simons syndrome (BSS), a rare form of acquired partial lipodystrophy. Complement Factor D (FD) is a serine protease with a crucial role in the activation of the alternative pathway of the complement system, which is mainly synthesized by adipose tissue. However, its role in the pathogenesis of BSS has not been addressed. In this study, plasma FD concentration was measured in 13 patients with BSS, 20 patients with acquired generalized lipodystrophy, 22 patients with C3 glomerulopathy (C3G), and 50 healthy controls. Gene expression and immunohistochemistry studies were assayed using atrophied adipose tissue from a patient with BSS. We found significantly elevated FD levels in BSS cases compared with the remaining cohorts (
MeSH term(s)	Adolescent ; Adult ; Aged ; Biomarkers/blood ; Case-Control Studies ; Child ; Cohort Studies ; Complement Factor D/metabolism ; Female ; Humans ; Lipodystrophy/blood ; Male ; Middle Aged ; Young Adult
Chemical Substances	Biomarkers ; CFD protein, human (EC 3.4.21.46) ; Complement Factor D (EC 3.4.21.46)
Language	English
Publishing date	2021-06-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22126608
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Article ; Online: Vitamin D status and supplementation before and after Bariatric Surgery: Recommendations based on a systematic review and meta-analysis.

Reviews in endocrine & metabolic disorders

2023 Volume 24, Issue 6, Page(s) 1011–1029

Abstract: Bariatric surgery is associated with a postoperative reduction of 25(OH) vitamin D levels (25(OH)D ... and with skeletal complications. Currently, guidelines for 25(OH)D assessment and vitamin D ... to analyse systematically the published experience on 25(OH)D status and vitamin D supplementation, pre- and post-surgery ...

Abstract	Bariatric surgery is associated with a postoperative reduction of 25(OH) vitamin D levels (25(OH)D) and with skeletal complications. Currently, guidelines for 25(OH)D assessment and vitamin D supplementation in bariatric patients, pre- and post-surgery, are still lacking. The aim of this work is to analyse systematically the published experience on 25(OH)D status and vitamin D supplementation, pre- and post-surgery, and to propose, on this basis, recommendations for management. Preoperatively, 18 studies including 2,869 patients were evaluated. Prevalence of vitamin D insufficiency as defined by 25(OH)D < 30 ng/mL (75 nmol/L) was 85%, whereas when defined by 25(OH)D < 20 ng/mL (50 nmol/L) was 57%. The median preoperative 25(OH)D level was 19.75 ng/mL. After surgery, 39 studies including 5,296 patients were analysed and among those undergoing either malabsorptive or restrictive procedures, a lower rate of vitamin D insufficiency and higher 25(OH)D levels postoperatively were observed in patients treated with high-dose oral vitamin D supplementation, defined as ≥ 2,000 IU/daily (mostly D3-formulation), compared with low-doses (< 2,000 IU/daily). Our recommendations based on this systematic review and meta-analysis should help clinical practice in the assessment and management of vitamin D status before and after bariatric surgery. Assessment of vitamin D should be performed pre- and postoperatively in all patients undergoing bariatric surgery. Regardless of the type of procedure, high-dose supplementation is recommended in patients after bariatric surgery.
MeSH term(s)	Humans ; Vitamin D ; Vitamin D Deficiency/drug therapy ; Vitamin D Deficiency/epidemiology ; Vitamin D Deficiency/etiology ; Dietary Supplements ; Vitamins/therapeutic use ; Bariatric Surgery
Chemical Substances	Vitamin D (1406-16-2) ; Vitamins
Language	English
Publishing date	2023-09-04
Publishing country	Germany
Document type	Meta-Analysis ; Systematic Review ; Journal Article ; Review
ZDB-ID	2185718-0
ISSN	1573-2606 ; 1389-9155
ISSN (online)	1573-2606
ISSN	1389-9155
DOI	10.1007/s11154-023-09831-3
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Article ; Online: Analysis of HIV quasispecies and virological outcome of an HIV D+/R+ kidney–liver transplantation

Rozera, Gabriella / Visco-Comandini, Ubaldo / Giombini, Emanuela / Santini, Francesco / Forbici, Federica / Berno, Giulia / Gruber, Cesare / De Paolis, Paolo / Colonnelli, Roberto / D’Offizi, Gianpiero / Ettorre, Giuseppe Maria / Grossi, Paolo / Capobianchi, Maria Rosaria / Ippolito, Giuseppe / Abbate, Isabella

Virol J. 2022 Dec., v. 19, no. 1 p.4-4

2022

Abstract: ... intervention. This study involves an HIV D+/R+ kidney–liver transplantation, where PBMC-associated HIV ...

Abstract	INTRODUCTION: Transplantation among HIV positive patients may be a valuable therapeutic intervention. This study involves an HIV D+/R+ kidney–liver transplantation, where PBMC-associated HIV quasispecies were analyzed in donor and transplant recipients (TR) prior to transplantation and thereafter, together with standard viral monitoring. METHODS: The donor was a 54 year of age HIV infected woman: kidney and liver recipients were two HIV infected men, aged 49 and 61. HIV quasispecies in PBMC was analyzed by ultra-deep sequencing of V3 env region. During TR follow-up, plasma HIV-1 RNA, HIV-1 DNA in PBMC, analysis of proviral integration sites and drug-resistance genotyping were performed. Other virological and immunological monitoring included CMV and EBV DNA quantification in blood and CD4 T cell counts. RESULTS: Donor and TR were all ART-HIV suppressed at transplantation. Thereafter, TR maintained a nearly suppressed HIV-1 viremia, but HIV-1 RNA blips and the increase of proviral integration sites in PBMC attested some residual HIV replication. A transient peak in HIV-1 DNA occurred in the liver recipient. No major changes of drug-resistance genotype were detected after transplantation. CMV and EBV transient reactivations were observed only in the kidney recipient, but did not require specific treatment. CD4 counts remained stable. No intermixed quasispecies between donor and TR was observed at transplantation or thereafter. Despite signs of viral evolution in TR, HIV genetic heterogeneity did not increase over the course of the months of follow up. CONCLUSIONS: No evidence of HIV superinfection was observed in the donor nor in the recipients. The immunosuppressive treatment administrated to TR did not result in clinical relevant viral reactivations.
Keywords	CD4-positive T-lymphocytes ; DNA ; RNA ; drug resistance ; evolution ; genetic heterogeneity ; genotype ; genotyping ; immunosuppression ; kidneys ; liver ; superinfection ; therapeutics ; viremia ; women
Language	English
Dates of publication	2022-12
Size	p. 4.
Publishing place	BioMed Central
Document type	Article ; Online
ZDB-ID	2160640-7
ISSN	1743-422X
ISSN	1743-422X
DOI	10.1186/s12985-021-01730-w
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Article ; Online: A key opinion leader interview with Prof D Santini: insight into cancer research.

Palmer, Claudia / Santini, Daniele

Expert opinion on therapeutic targets

2015 Volume 19, Issue 3, Page(s) 443–445

MeSH term(s)	Antineoplastic Agents/pharmacology ; Biomedical Research/methods ; Humans ; Molecular Targeted Therapy ; Neoplasms/pathology ; Neoplasms/therapy
Chemical Substances	Antineoplastic Agents
Language	English
Publishing date	2015-03
Publishing country	England
Document type	Interview
ZDB-ID	2055208-7
ISSN	1744-7631 ; 1472-8222
ISSN (online)	1744-7631
ISSN	1472-8222
DOI	10.1517/14728222.2015.1015227
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