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  1. Article ; Online: Nanopore-Based Direct RNA Sequencing of the

    Kruse, Elisabeth / Göringer, H Ulrich

    Genes

    2023  Volume 14, Issue 3

    Abstract: Trypanosomatids are single-cell eukaryotic parasites. Unlike higher eukaryotes, they control gene expression post-transcriptionally and not at the level of transcription initiation. This involves all known cellular RNA circuits, from mRNA processing to ... ...

    Abstract Trypanosomatids are single-cell eukaryotic parasites. Unlike higher eukaryotes, they control gene expression post-transcriptionally and not at the level of transcription initiation. This involves all known cellular RNA circuits, from mRNA processing to mRNA decay, to translation, in addition to a large panel of RNA-interacting proteins that modulate mRNA abundance. However, other forms of gene regulation, for example by lncRNAs, cannot be excluded. LncRNAs are poorly studied in trypanosomatids, with only a single lncRNA characterized to date. Furthermore, it is not clear whether the complete inventory of trypanosomatid lncRNAs is known, because of the inherent cDNA-recoding and DNA-amplification limitations of short-read RNA sequencing. Here, we overcome these limitations by using long-read direct RNA sequencing (DRS) on nanopore arrays. We analyze the native RNA pool of the two main lifecycle stages of the African trypanosome
    MeSH term(s) Transcriptome/genetics ; Trypanosoma brucei brucei/genetics ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; Nanopores ; RNA, Messenger/genetics ; Sequence Analysis, RNA
    Chemical Substances RNA, Long Noncoding ; RNA, Messenger
    Language English
    Publishing date 2023-02-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14030610
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Online: Making the Geologic Now: Responses to Material Conditions of Contemporary Life

    Kruse, Jamie / Ellsworth, Elisabeth

    2012  

    Abstract: Making the Geologic Now announces shifts in cultural sensibilities and practices. It offers early sightings of an increasingly widespread turn toward the geologic as source of explanation, motivation, and inspiration for creative responses to conditions ... ...

    Abstract Making the Geologic Now announces shifts in cultural sensibilities and practices. It offers early sightings of an increasingly widespread turn toward the geologic as source of explanation, motivation, and inspiration for creative responses to conditions of the present moment. In the spirit of a broadside, this edited collection circulates images and short essays from over 40 artists, designers, architects, scholars, and journalists who are actively exploring and creatively responding to the geologic depth of "now." Contributors' ideas and works are drawn from architecture, design, contemporary philosophy and art. They are offered as test sites for what might become thinkable or possible if humans were to collectively take up the geologic as our instructive co-designer-as a partner in designing thoughts, objects, systems, and experiences. Recent natural and human-made events triggered by or triggering the geologic have made volatile earth forces sense-able and relevant with new levels of intensity. As a condition of contemporary life in 2012, the geologic "now" is lived as a cascade of events. Humans and what we build participate in their unfolding. Today, and unlike the environmental movements of the 1970s, the geologic counts as "the environment" and invites us to extend our active awareness of inhabitation out to the cosmos and down to the Earth's iron core
    Keywords Geology
    Size 1 electronic resource (262 p.)
    Publisher punctum books
    Publishing place Verlagsort nicht ermittelbar
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT020195687
    ISBN 9780615766362 ; 0615766366
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  3. Article ; Online: Nanopore-Based Direct RNA Sequencing of the Trypanosoma brucei Transcriptome Identifies Novel lncRNAs

    Kruse, Elisabeth / Göringer, H. Ulrich

    Genes (Basel). 2023 Feb. 28, v. 14, no. 3

    2023  

    Abstract: Trypanosomatids are single-cell eukaryotic parasites. Unlike higher eukaryotes, they control gene expression post-transcriptionally and not at the level of transcription initiation. This involves all known cellular RNA circuits, from mRNA processing to ... ...

    Abstract Trypanosomatids are single-cell eukaryotic parasites. Unlike higher eukaryotes, they control gene expression post-transcriptionally and not at the level of transcription initiation. This involves all known cellular RNA circuits, from mRNA processing to mRNA decay, to translation, in addition to a large panel of RNA-interacting proteins that modulate mRNA abundance. However, other forms of gene regulation, for example by lncRNAs, cannot be excluded. LncRNAs are poorly studied in trypanosomatids, with only a single lncRNA characterized to date. Furthermore, it is not clear whether the complete inventory of trypanosomatid lncRNAs is known, because of the inherent cDNA-recoding and DNA-amplification limitations of short-read RNA sequencing. Here, we overcome these limitations by using long-read direct RNA sequencing (DRS) on nanopore arrays. We analyze the native RNA pool of the two main lifecycle stages of the African trypanosome Trypanosoma brucei, with a special emphasis on the inventory of lncRNAs. We identify 207 previously unknown lncRNAs, 32 of which are stage-specifically expressed. We also present insights into the complexity of the T. brucei transcriptome, including alternative transcriptional start and stop sites and potential transcript isoforms, to provide a bias-free understanding of the intricate RNA landscape in T. brucei.
    Keywords RNA ; Trypanosoma brucei ; eukaryotic cells ; gene expression ; genes ; inventories ; nanopores ; transcription initiation ; transcriptome
    Language English
    Dates of publication 2023-0228
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14030610
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Simulating long-term wildfire impacts on boreal forest structure in Central Yakutia, Siberia, since the Last Glacial Maximum.

    Glückler, Ramesh / Gloy, Josias / Dietze, Elisabeth / Herzschuh, Ulrike / Kruse, Stefan

    Fire ecology

    2024  Volume 20, Issue 1, Page(s) 1

    Abstract: Background: Wildfires are recognized as an important ecological component of larch-dominated boreal forests in eastern Siberia. However, long-term fire-vegetation dynamics in this unique environment are poorly understood. Recent paleoecological research ...

    Abstract Background: Wildfires are recognized as an important ecological component of larch-dominated boreal forests in eastern Siberia. However, long-term fire-vegetation dynamics in this unique environment are poorly understood. Recent paleoecological research suggests that intensifying fire regimes may induce millennial-scale shifts in forest structure and composition. This may, in turn, result in positive feedback on intensifying wildfires and permafrost degradation, apart from threatening human livelihoods. Most common fire-vegetation models do not explicitly include detailed individual-based tree population dynamics, but a focus on patterns of forest structure emerging from interactions among individual trees may provide a beneficial perspective on the impacts of changing fire regimes in eastern Siberia. To simulate these impacts on forest structure at millennial timescales, we apply the individual-based, spatially explicit vegetation model LAVESI-FIRE, expanded with a new fire module. Satellite-based fire observations along with fieldwork data were used to inform the implementation of wildfire occurrence and adjust model parameters.
    Results: Simulations of annual forest development and wildfire activity at a study site in the Republic of Sakha (Yakutia) since the Last Glacial Maximum (c. 20,000 years BP) highlight the variable impacts of fire regimes on forest structure throughout time. Modeled annual fire probability and subsequent burned area in the Holocene compare well with a local reconstruction of charcoal influx in lake sediments. Wildfires can be followed by different forest regeneration pathways, depending on fire frequency and intensity and the pre-fire forest conditions. We find that medium-intensity wildfires at fire return intervals of 50 years or more benefit the dominance of fire-resisting Dahurian larch (
    Conclusion: With its fine-scale population dynamics, LAVESI-FIRE can serve as a highly localized, spatially explicit tool to understand the long-term impacts of boreal wildfires on forest structure and to better constrain interpretations of paleoecological reconstructions of fire activity.
    Supplementary information: The online version contains supplementary material available at 10.1186/s42408-023-00238-8.
    Language English
    Publishing date 2024-01-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2575363-0
    ISSN 1933-9747 ; 1933-9747
    ISSN (online) 1933-9747
    ISSN 1933-9747
    DOI 10.1186/s42408-023-00238-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Trypanosomatid, fluorescence-based

    Leeder, Wolf-Matthias / Kruse, Elisabeth / Göringer, H Ulrich

    Bio-protocol

    2021  Volume 11, Issue 5, Page(s) e3935

    Abstract: Gene expression within the mitochondria of African trypanosomes and other protozoan organisms relies on a nucleotide-specific RNA-editing reaction. In the process exclusively uridine (U)-nucleotides are site-specifically inserted into and deleted from ... ...

    Abstract Gene expression within the mitochondria of African trypanosomes and other protozoan organisms relies on a nucleotide-specific RNA-editing reaction. In the process exclusively uridine (U)-nucleotides are site-specifically inserted into and deleted from sequence-deficient primary transcripts to convert them into translatable mRNAs. The reaction is catalyzed by a 0.8 MDa multiprotein complex termed the editosome. Here we describe an improved
    Language English
    Publishing date 2021-03-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.3935
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book ; Thesis: The role of nuclear protein matrix in drug induced cell death

    Kruse, Elisabeth

    1989  

    Size Getr. Zählung : Ill., graph. Darst.
    Publishing country Sweden
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Lund, Univ., Diss., 1989
    HBZ-ID HT003298198
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    89 E 1166 order for loan Magazin

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  7. Book ; Thesis: Untersuchungen zur Frage einer Glykoproteinnatur des Östradiolrezeptors

    Kruse, Elisabeth

    1: Isolierung u. Charakterisierung d. Peptid:N-Glykosidase aus Mandeln ; 2: Empfindlichkeit d. Östradiolrezeptors gegen Glykosidasen u. sein Verhalten zu Lektinen

    (Schriftenreihe / Max-Planck-Institut für Experimentelle Endokrinologie ; 3)

    1987  

    Series title Schriftenreihe / Max-Planck-Institut für Experimentelle Endokrinologie ; 3
    Collection
    Size 100 S. : Ill., graph. Darst.
    Publisher Max-Planck-Inst. für Experimentelle Endokrinologie
    Publishing place Hannover
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Hannover, Univ., Diss., 1986
    HBZ-ID HT003230636
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    89 D 229 order for loan Magazin

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  8. Article ; Online: Impact of Maternal Smoking on Nonsyndromic Clefts: Sex-Specific Associations With Side and Laterality.

    Kruse, Teresa / Mangold, Elisabeth / Braumann, Bert

    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association

    2020  Volume 58, Issue 2, Page(s) 181–188

    Abstract: Objective: To compare the incidence of right-sided versus left-sided, and unilateral versus bilateral, nonsyndromic clefting in the affected offspring of smoking and nonsmoking mothers.: Design: Self-report data on periconceptual and first trimester ... ...

    Abstract Objective: To compare the incidence of right-sided versus left-sided, and unilateral versus bilateral, nonsyndromic clefting in the affected offspring of smoking and nonsmoking mothers.
    Design: Self-report data on periconceptual and first trimester smoking behavior were collected from 842 mothers of children with nonsyndromic orofacial clefting. Differences in the incidence of left- versus right-sided clefts, and of unilateral versus bilateral clefts, were analyzed between the children of smoking and nonsmoking mothers.
    Setting: Interviews and clinical examinations took place at 8 specialist centers in Germany.
    Patients and participants: Children with nonsyndromic clefts were recruited during the course of surgical or orthodontic treatment, or within the context of the annual control consultation. Patients with cleft palate only or missing data were excluded. The final cohort comprised 842 patients (540 males and 302 females) with unilateral or bilateral clefts. The respective mothers were interviewed.
    Main outcome measure: Side and laterality of nonsyndromic clefts were the main outcome measures.
    Results: Children of smoking mothers more often had right-sided clefts than children of nonsmoking mothers (42% right-sided clefts in children of smoking mothers vs 31% of nonsmoking mothers). Children of smoking mothers more often had bilateral clefts than children of nonsmoking mothers (35% bilateral clefts in children of smoking mothers vs 29% of nonsmoking mothers). Sex-specific analyses confirmed substantially and statistically significant associations only for girls.
    Conclusions: The results suggest that maternal smoking is a sex-specific, exogenous determinant of laterality and side in nonsyndromic clefts.
    MeSH term(s) Child ; Cleft Lip/epidemiology ; Cleft Lip/etiology ; Cleft Palate/epidemiology ; Cohort Studies ; Female ; Germany/epidemiology ; Humans ; Male ; Smoking
    Language English
    Publishing date 2020-08-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1069409-2
    ISSN 1545-1569 ; 0009-8701 ; 1055-6656
    ISSN (online) 1545-1569
    ISSN 0009-8701 ; 1055-6656
    DOI 10.1177/1055665620951099
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 451: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Book: Einsatz der ELISA- und Schnellagglutinationstechnik zur Diagnose von Salmonella enteritidis

    Vasicek, Lore / Kruse, Elisabeth

    Forschungsbericht

    (Beiträge Lebensmittelangelegenheiten, Veterinärverwaltung, Strahlenschutz, Toxikologie, Gentechnik ; 95,7)

    1995  

    Author's details L. Vasicek ; E. Kruse
    Series title Beiträge Lebensmittelangelegenheiten, Veterinärverwaltung, Strahlenschutz, Toxikologie, Gentechnik ; 95,7
    Collection
    Language German
    Size 37 S.
    Publisher Bundesministerium für Gesundheit und Konsumentenschutz, Sekt. III
    Publishing place Wien
    Publishing country Austria
    Document type Book
    Note Literaturverz. S. 34 - 37
    HBZ-ID HT014405146
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    1999 A 8060 order for loan Magazin

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  10. Article ; Online: Der „Hamburger Hausbesuch für Seniorinnen und Senioren“ : Entwicklung, Durchführung und Akzeptanz bei 4716 älteren Menschen in 15 Monaten.

    Neumann, Lilli / Dapp, Ulrike / Böttcher-Völker, Silke / Kleinhans, Elisabeth / von Renteln-Kruse, Wolfgang

    Zeitschrift fur Gerontologie und Geriatrie

    2021  Volume 54, Issue 5, Page(s) 471–478

    Abstract: Background: Preventive home visits are part of communal programs for older citizens. The city of Hamburg promoted the pilot project "The Hamburg home visit for older citizens" in two city districts. The voluntary program is offered to persons on their ... ...

    Title translation The "Hamburg home visit for older citizens" : Development, execution and acceptance in 4716 older people in 15 months.
    Abstract Background: Preventive home visits are part of communal programs for older citizens. The city of Hamburg promoted the pilot project "The Hamburg home visit for older citizens" in two city districts. The voluntary program is offered to persons on their 80th birthday, without comprehensive assessment and is performed by visitors with a background in healthcare or social work. The aim is to provide information, to identify need of help and to initiate contact with officials for support if wanted.
    Methods: The structural and administrative prerequisites were defined together with officials of the city of Hamburg and the two city districts. The data safety concept, provision of addresses, birthday letter, information to be provided, recruitment, qualification and training of the visitors, timing and performance of visits, guiding how to communicate during the home visit, documentation and quarterly reports are described in detail.
    Results: From September 2018 to December 2019, 1636 (35%) out of 4716 persons contacted were visited by 59 visitors. The most frequent reasons for refusal were no need because of good health (40%) or satisfaction with existing support (14%). Most issues addressed were health situation (51%), mobility (43%), housing conditions (42%) and social contacts (41%). There was need of support as documented in 399 out of 1636 home visits, and contacts for support were initiated as required by 55 persons.
    Conclusion: The acceptance of this new approach was 10% higher than 25% per month that had been expected in both the concentrated urban as well as the more rural city districts. Home visits on the 80th birthday appear to be feasible provided that structural and operational prerequisites are considered. In 2020, they were offered to all districts of the city, i.e. to about 15,000 persons per year.
    MeSH term(s) Aged ; Geriatric Assessment ; House Calls ; Humans ; Life Style ; Pilot Projects ; Preventive Health Services
    Language German
    Publishing date 2021-04-01
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1227032-5
    ISSN 1435-1269 ; 0044-281X ; 0948-6704
    ISSN (online) 1435-1269
    ISSN 0044-281X ; 0948-6704
    DOI 10.1007/s00391-021-01878-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 497: Show issues Location:
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