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  1. Article ; Online: The assembly of a spliceosomal small nuclear ribonucleoprotein particle.

    Patel, Snehal Bhikhu / Bellini, Michel

    Nucleic acids research

    2008  Volume 36, Issue 20, Page(s) 6482–6493

    Abstract: The U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles (snRNPs) are essential elements of the spliceosome, the enzyme that catalyzes the excision of introns and the ligation of exons to form a mature mRNA. Since their discovery over a ... ...

    Abstract The U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles (snRNPs) are essential elements of the spliceosome, the enzyme that catalyzes the excision of introns and the ligation of exons to form a mature mRNA. Since their discovery over a quarter century ago, the structure, assembly and function of spliceosomal snRNPs have been extensively studied. Accordingly, the functions of splicing snRNPs and the role of various nuclear organelles, such as Cajal bodies (CBs), in their nuclear maturation phase have already been excellently reviewed elsewhere. The aim of this review is, then, to briefly outline the structure of snRNPs and to synthesize new and exciting developments in the snRNP biogenesis pathways.
    MeSH term(s) RNA Caps/metabolism ; Ribonucleoprotein, U4-U6 Small Nuclear/metabolism ; Ribonucleoproteins, Small Nuclear/chemistry ; Ribonucleoproteins, Small Nuclear/metabolism ; Spliceosomes/chemistry ; Spliceosomes/metabolism
    Chemical Substances RNA Caps ; Ribonucleoprotein, U4-U6 Small Nuclear ; Ribonucleoproteins, Small Nuclear
    Language English
    Publishing date 2008-11
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 2205588-5
    ISSN 1362-4962 ; 1746-8272 ; 0305-1048 ; 0261-3166
    ISSN (online) 1362-4962 ; 1746-8272
    ISSN 0305-1048 ; 0261-3166
    DOI 10.1093/nar/gkn658
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The assembly of a spliceosomal small nuclear ribonucleoprotein particle

    Patel, Snehal Bhikhu / Bellini, Michel

    Nucleic acids research. 2008 Nov., v. 36, no. 20

    2008  

    Abstract: The U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles (snRNPs) are essential elements of the spliceosome, the enzyme that catalyzes the excision of introns and the ligation of exons to form a mature mRNA. Since their discovery over a ... ...

    Abstract The U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles (snRNPs) are essential elements of the spliceosome, the enzyme that catalyzes the excision of introns and the ligation of exons to form a mature mRNA. Since their discovery over a quarter century ago, the structure, assembly and function of spliceosomal snRNPs have been extensively studied. Accordingly, the functions of splicing snRNPs and the role of various nuclear organelles, such as Cajal bodies (CBs), in their nuclear maturation phase have already been excellently reviewed elsewhere. The aim of this review is, then, to briefly outline the structure of snRNPs and to synthesize new and exciting developments in the snRNP biogenesis pathways.
    Keywords biogenesis ; exons ; introns ; messenger RNA ; ribonucleoproteins ; spliceosomes
    Language English
    Dates of publication 2008-11
    Size p. 6482-6493.
    Document type Article
    ZDB-ID 186809-3
    ISSN 0301-5610 ; 0305-1048
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkn658
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 79/704: Show issues

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  3. Article: Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts

    Patel, Snehal Bhikhu / Novikova, Natalya / Bellini, Michel

    Journal of cell biology. 2007 Sept. 10, v. 178, no. 6

    2007  

    Abstract: In amphibian oocytes, most lateral loops of the lampbrush chromosomes correspond to active transcriptional sites for RNA polymerase II. We show that newly assembled small nuclear ribonucleoprotein (RNP [snRNP]) particles, which are formed upon ... ...

    Abstract In amphibian oocytes, most lateral loops of the lampbrush chromosomes correspond to active transcriptional sites for RNA polymerase II. We show that newly assembled small nuclear ribonucleoprotein (RNP [snRNP]) particles, which are formed upon cytoplasmic injection of fluorescently labeled spliceosomal small nuclear RNAs (snRNAs), target the nascent transcripts of the chromosomal loops. With this new targeting assay, we demonstrate that nonfunctional forms of U1 and U2 snRNAs still associate with the active transcriptional units. In particular, we find that their association with nascent RNP fibrils is independent of their base pairing with pre-messenger RNAs. Additionally, stem loop I of the U1 snRNA is identified as a discrete domain that is both necessary and sufficient for association with nascent transcripts. Finally, in oocytes deficient in splicing, the recruitment of U1, U4, and U5 snRNPs to transcriptional units is not affected. Collectively, these data indicate that the recruitment of snRNPs to nascent transcripts and the assembly of the spliceosome are uncoupled events.
    Language English
    Dates of publication 2007-0910
    Size p. 937-949.
    Publishing place The Rockefeller University Press
    Document type Article
    ZDB-ID 218154-x
    ISSN 1540-8140 ; 0021-9525
    ISSN (online) 1540-8140
    ISSN 0021-9525
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 56/32: Show issues

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  4. Article: Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts.

    Patel, Snehal Bhikhu / Novikova, Natalya / Bellini, Michel

    The Journal of cell biology

    2007  Volume 178, Issue 6, Page(s) 937–949

    Abstract: In amphibian oocytes, most lateral loops of the lampbrush chromosomes correspond to active transcriptional sites for RNA polymerase II. We show that newly assembled small nuclear ribonucleoprotein (RNP [snRNP]) particles, which are formed upon ... ...

    Abstract In amphibian oocytes, most lateral loops of the lampbrush chromosomes correspond to active transcriptional sites for RNA polymerase II. We show that newly assembled small nuclear ribonucleoprotein (RNP [snRNP]) particles, which are formed upon cytoplasmic injection of fluorescently labeled spliceosomal small nuclear RNAs (snRNAs), target the nascent transcripts of the chromosomal loops. With this new targeting assay, we demonstrate that nonfunctional forms of U1 and U2 snRNAs still associate with the active transcriptional units. In particular, we find that their association with nascent RNP fibrils is independent of their base pairing with pre-messenger RNAs. Additionally, stem loop I of the U1 snRNA is identified as a discrete domain that is both necessary and sufficient for association with nascent transcripts. Finally, in oocytes deficient in splicing, the recruitment of U1, U4, and U5 snRNPs to transcriptional units is not affected. Collectively, these data indicate that the recruitment of snRNPs to nascent transcripts and the assembly of the spliceosome are uncoupled events.
    MeSH term(s) Animals ; Female ; Nucleic Acid Conformation ; RNA Polymerase II/genetics ; RNA Polymerase II/metabolism ; RNA Precursors/genetics ; RNA Precursors/metabolism ; RNA Splicing ; RNA, Small Nuclear/genetics ; RNA, Small Nuclear/metabolism ; Ribonucleoprotein, U1 Small Nuclear/genetics ; Ribonucleoprotein, U1 Small Nuclear/metabolism ; Ribonucleoprotein, U2 Small Nuclear/genetics ; Ribonucleoprotein, U2 Small Nuclear/metabolism ; Ribonucleoprotein, U4-U6 Small Nuclear/genetics ; Ribonucleoprotein, U4-U6 Small Nuclear/metabolism ; Ribonucleoprotein, U5 Small Nuclear/genetics ; Ribonucleoprotein, U5 Small Nuclear/metabolism ; Ribonucleoprotein, U7 Small Nuclear/genetics ; Ribonucleoprotein, U7 Small Nuclear/metabolism ; Ribonucleoproteins, Small Nuclear/genetics ; Ribonucleoproteins, Small Nuclear/metabolism ; Spliceosomes/genetics ; Spliceosomes/physiology ; Xenopus
    Chemical Substances RNA Precursors ; RNA, Small Nuclear ; Ribonucleoprotein, U1 Small Nuclear ; Ribonucleoprotein, U2 Small Nuclear ; Ribonucleoprotein, U4-U6 Small Nuclear ; Ribonucleoprotein, U5 Small Nuclear ; Ribonucleoprotein, U7 Small Nuclear ; Ribonucleoproteins, Small Nuclear ; RNA Polymerase II (EC 2.7.7.-)
    Language English
    Publishing date 2007-09-10
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 218154-x
    ISSN 1540-8140 ; 0021-9525
    ISSN (online) 1540-8140
    ISSN 0021-9525
    DOI 10.1083/jcb.200706134
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Ub I Zs.190: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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