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  1. Book: Brock Mikrobiologie

    Madigan, Michael T. / Bender, Kelly S. / Buckley, Daniel H. / Sattley, W. Matthew / Stahl, David A. / Brock, Thomas D. / Wirth, Reinhard

    (Pearson - Biologie)

    2020  

    Title translation Brock biology of microorganisms
    Title variant Mikrobiologie
    Author's details Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl ; Übersetzung und Fachlektorat: Reinhard Wirth
    Series title Pearson - Biologie
    Keywords Virologie ; Biologie ; Stoffwechsel ; Molekularbiologie ; Mikrobiologie
    Subject code 570
    Language German
    Size XLVII, 1388 Seiten, Illustrationen, Diagramme
    Edition 15., aktualisierte Auflage
    Publisher Pearson Deutschland GmbH
    Publishing place München
    Publishing country Germany
    Document type Book
    Note Authorized translation of the English languange edition
    HBZ-ID HT020172648
    ISBN 978-3-86894-367-2 ; 3-86894-367-6 ; 9783863268688 ; 3863268687
    Database Catalogue ZB MED Medicine, Health

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  2. Book: Handbuch Intensivpflege

    Bathe, Isabel / Brock, Andrea

    medizinische und pflegerische Grundlagen ; [www.pflegeheute.de]

    2014  

    Author's details Andrea Brock ... (Hrsg.) Mit Beitr. von: Isabel Bathe
    Keywords Critical Care ; Intensivpflege
    Subject Critical care nursing
    Language German
    Size VII, 376 S. : zahlr. Ill.
    Edition 1. Aufl.
    Publisher Elsevier, Urban & Fischer
    Publishing place München
    Publishing country Germany
    Document type Book
    New title 2. Aufl. u.d.T. Fachpflege Intensivpflege
    HBZ-ID HT018244236
    ISBN 978-3-437-25212-9 ; 3-437-25212-7 ; 9783437297908 ; 3437297902
    Database Catalogue ZB MED Medicine, Health

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  3. Article: V(D)J recombinase-mediated deletion of the hprt gene in T-lymphocytes from adult humans.

    Fuscoe, J C / Zimmerman, L J / Harrington-Brock, K / Burnette, L / Moore, M M / Nicklas, J A / O'Neill, J P / Albertini, R J

    Mutation research

    1992  Volume 283, Issue 1, Page(s) 13–20

    Abstract: ... in the hypoxanthine-guanine phosphoribosyltransferase (hprt) gene of T-lymphocytes. We have shown previously that the illegitimate activity of V(D)J ... The hprt T-cell cloning assay allows the detection of mutations occurring in vivo ... recombinase accounts for about 40% of the hprt mutations in T-lymphocytes of human newborns as measured ...

    Abstract The hprt T-cell cloning assay allows the detection of mutations occurring in vivo in the hypoxanthine-guanine phosphoribosyltransferase (hprt) gene of T-lymphocytes. We have shown previously that the illegitimate activity of V(D)J recombinase accounts for about 40% of the hprt mutations in T-lymphocytes of human newborns as measured with umbilical cord blood samples (Fuscoe et al., 1991). This mechanism results in deletion of hprt exons 2 + 3. In this report, we examined a collection of 314 HPRT-deficient clones derived from adult humans for evidence that the mutations were caused by this mechanism by analyzing exons 2 + 3 deletion mutations. DNA sequence analysis of deletion breakpoint junctions showed that 8 of the mutations were the result of V(D)J recombinase activity. The frequency of the recombinase-mediated mutations was similar in the adults and newborns (2-4 x 10(-7). However, since the hprt mutant frequency is about 10-fold higher in the adult than in the newborn, the recombinase-mediated mutations account for only a few percent of the adult mutations. These mutations are likely to have occurred during early development and persist into adulthood. Unregulated expression of V(D)J recombinase activity may be an important mechanism for genomic rearrangements in the genesis of cancer.
    MeSH term(s) Adult ; Base Sequence ; Chromosome Deletion ; Clone Cells ; DNA/genetics ; DNA Nucleotidyltransferases/metabolism ; Exons ; Genes ; Humans ; Hypoxanthine Phosphoribosyltransferase/genetics ; Introns ; Male ; Molecular Sequence Data ; Oligodeoxyribonucleotides ; Polymerase Chain Reaction/methods ; T-Lymphocytes/enzymology ; VDJ Recombinases
    Chemical Substances Oligodeoxyribonucleotides ; DNA (9007-49-2) ; Hypoxanthine Phosphoribosyltransferase (EC 2.4.2.8) ; DNA Nucleotidyltransferases (EC 2.7.7.-) ; VDJ Recombinases (EC 2.7.7.-)
    Language English
    Publishing date 1992-09
    Publishing country Netherlands
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 206607-5
    ISSN 1873-135X ; 0027-5107 ; 1383-5718 ; 0165-1110 ; 0165-1161 ; 0165-7992 ; 0921-8777 ; 0165-1218 ; 1383-5726 ; 0167-8817 ; 0921-8734 ; 1383-5742
    ISSN (online) 1873-135X
    ISSN 0027-5107 ; 1383-5718 ; 0165-1110 ; 0165-1161 ; 0165-7992 ; 0921-8777 ; 0165-1218 ; 1383-5726 ; 0167-8817 ; 0921-8734 ; 1383-5742
    DOI 10.1016/0165-7992(92)90116-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Measuring β-galactosidase activity in opaque dairy solutions under optimum conditions for galactooligosaccharide synthesis by isothermal titration calorimetry.

    Jarrard, T R / Brock, E / Hansen, L D / Kenealey, J D

    Journal of dairy science

    2023  Volume 106, Issue 12, Page(s) 8312–8320

    Abstract: The dairy industry uses enzymes to make cheese, alter product flavor, and eliminate lactose. The activities of these enzymes have been measured in clear buffered solutions, but because of the limitations of spectrophotometric methods, enzyme activities ... ...

    Abstract The dairy industry uses enzymes to make cheese, alter product flavor, and eliminate lactose. The activities of these enzymes have been measured in clear buffered solutions, but because of the limitations of spectrophotometric methods, enzyme activities have not been measured in opaque or colored dairy products where they are used. Isothermal titration calorimetry (ITC) can be used to determine reaction kinetics in opaque and colored solutions by measuring the heat rate (thermal power) from enzyme-catalyzed reactions as a function of time. This study used ITC to measure β-galactosidase activity in opaque solutions of milk, sweet whey, sweet whey permeate, acid whey, and acid whey permeate with 2 β-galactosidase (Enzyme Commission number 3.2.1.23) isozymes derived from Aspergillus oryzae and Kluyveromyces lactis. The components of the dairy fluids alter the enzyme kinetics and reaction thermodynamics, and the reactions catalyzed by the 2 homologues differ as shown by differing thermodynamic profiles. The study demonstrates that ITC can be used to measure enzyme activity in opaque and colored dairy fluids and identify reactions by their thermodynamic properties.
    MeSH term(s) Animals ; Calorimetry/veterinary ; Milk ; Whey/chemistry ; Cheese ; beta-Galactosidase/chemistry ; Lactose/analysis
    Chemical Substances beta-Galactosidase (EC 3.2.1.23) ; Lactose (J2B2A4N98G)
    Language English
    Publishing date 2023-08-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 242499-x
    ISSN 1525-3198 ; 0022-0302
    ISSN (online) 1525-3198
    ISSN 0022-0302
    DOI 10.3168/jds.2023-23400
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: The Present and Future of Virtual Reality in Medical Education: A Narrative Review.

    Mistry, Dipal / Brock, Callaham A / Lindsey, Tom

    Cureus

    2023  Volume 15, Issue 12, Page(s) e51124

    Abstract: Virtual reality (VR) uses computer-generated and three-dimensional environments to create immersive experiences through the use of interactive devices that simulate virtual environments in many forms, such as 3D, screen-based, or room-based. Users can ... ...

    Abstract Virtual reality (VR) uses computer-generated and three-dimensional environments to create immersive experiences through the use of interactive devices that simulate virtual environments in many forms, such as 3D, screen-based, or room-based. Users can engage in the environment with objects, characters, and scenes, making individuals assume they are experiencing a real-life scenario. VR has been adopted across medical and nursing fields to supplement clinically relevant and practical teaching. However, the effectiveness of this interactive form of learning has come a long way with improvements in accessibility, cost, and technicalities. The immersive simulated environment that VR has to offer today initially began with screen-based learning and then the 360-video method. These previously sought-out methods were eventually found to disconnect the students from engaging in the learning environment that present-day VR systems are designed to provide. Interactive VR offers a dynamic platform for medical training. These simulations benefit the learner by allowing them to interact within case scenarios and virtual wards, as well as with patients, colleagues, and relatives. To mimic real-life encounters, the student can take a patient's history and physical exam, investigate, diagnose, and provide treatment. The simulated patient can express emotions, concerns, and signs of a poor state of health. All these factors play into a healthcare provider's competency to think critically and clinically in decision-making. This practice is now being used in many surgical programs and medical education curricula. The use of simulation in VR is continuously being proven to decrease injury, increase operation speed, and improve overall outcomes in patient-centered care. VR simulation differs from in-person simulation training in that the VR modality of learning is more accessible and replicable than the latter. By comparing research studies and reviews of medical programs that incorporated VR into their curricula, we were able to assess the state of VR in medical education and where this technology could lead to future implementation in medical programs. Our review aimed to give insight into the existing evidence, the gaps in the use of VR in medical education, and the potential benefits this modality of learning can have going forward in this field of study. Medical students have demonstrated significantly enhanced knowledge gain when using immersive interactive VR over screen-based learning. Given the improvements in students' performance due to these dynamic and collaborative learning experiences, immersive VR training will become a standard in the development of clinical skills and ensure patient safety. Although the emphasis on empathy began later in the journey of gaining VR as a part of medical education, there is a need to gain those skills as early as possible in medical school. Implementing the use of VR as a supplement in medical education allows students to practice simulated patient encounters along with an array of different academic endeavors. By doing so, students will gain competency and confidence as they encounter patients during their clinical rotations and clinical practice.
    Language English
    Publishing date 2023-12-26
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.51124
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Deep Learning Applications in Pancreatic Cancer.

    Patel, Hardik / Zanos, Theodoros / Hewitt, D Brock

    Cancers

    2024  Volume 16, Issue 2

    Abstract: Pancreatic cancer is one of the most lethal gastrointestinal malignancies. Despite advances in cross-sectional imaging, chemotherapy, radiation therapy, and surgical techniques, the 5-year overall survival is only 12%. With the advent and rapid adoption ... ...

    Abstract Pancreatic cancer is one of the most lethal gastrointestinal malignancies. Despite advances in cross-sectional imaging, chemotherapy, radiation therapy, and surgical techniques, the 5-year overall survival is only 12%. With the advent and rapid adoption of AI across all industries, we present a review of applications of DL in the care of patients diagnosed with PC. A review of different DL techniques with applications across diagnosis, management, and monitoring is presented across the different pathological subtypes of pancreatic cancer. This systematic review highlights AI as an emerging technology in the care of patients with pancreatic cancer.
    Language English
    Publishing date 2024-01-19
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers16020436
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Monotocy and the evolution of plural breeding in mammals.

    Lukas, Dieter / Clutton-Brock, Tim

    Behavioral ecology : official journal of the International Society for Behavioral Ecology

    2020  Volume 31, Issue 4, Page(s) 943–949

    Abstract: In many mammals, breeding females are intolerant of each other and seldom associate closely but, in some, they aggregate in groups that vary in size, stability, and kinship structure. Aggregation frequently increases competition for food, and ... ...

    Abstract In many mammals, breeding females are intolerant of each other and seldom associate closely but, in some, they aggregate in groups that vary in size, stability, and kinship structure. Aggregation frequently increases competition for food, and interspecific differences in female sociality among mammals are commonly attributed to contrasts in ecological parameters, including variation in activity timing, the distribution of resources, as well as the risk of predation. However, there is increasing indication that differences in female sociality are also associated with phylogenetic relationships and with contrasts in life-history parameters. We show here that evolutionary transitions from systems where breeding females usually occupy separate ranges ("singular breeding") to systems where breeding females usually aggregate ("plural breeding") have occurred more frequently in monotocous lineages where females produce single young than in polytocous ones where they produce litters. A likely explanation of this association is that competition between breeding females for resources is reduced where they produce single young and is more intense where they produce litters. Our findings reinforce evidence that variation in life-history parameters plays an important role in shaping the evolution of social behavior.
    Language English
    Publishing date 2020-05-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1496189-1
    ISSN 1465-7279 ; 1045-2249
    ISSN (online) 1465-7279
    ISSN 1045-2249
    DOI 10.1093/beheco/araa039
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: PDPR

    Brock, Pamela / Sevigny, Myriam / Liyanarachchi, Sandya / Comiskey, Daniel F / Li, Wei / Saarinen, Saila / Yilmaz, Ayse Selen / Nieminen, Anni I / Ringel, Matthew D / Peltomäki, Päivi / Ollila, Saara / Nieminen, Taina T

    Thyroid : official journal of the American Thyroid Association

    2024  

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2024-01-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1086044-7
    ISSN 1557-9077 ; 1050-7256
    ISSN (online) 1557-9077
    ISSN 1050-7256
    DOI 10.1089/thy.2023.0560
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: The effect of water potential on photosynthesis in whole lichens and in their liberated algal components.

    Brock, T D

    Planta

    2014  Volume 124, Issue 1, Page(s) 13–23

    Abstract: Experiments were performed to determine whether the fungal component of the lichen thallus might enable the algal component to function under conditions of moisture stress at which it would be unable to function if free-living. Four lichens were used: ... ...

    Abstract Experiments were performed to determine whether the fungal component of the lichen thallus might enable the algal component to function under conditions of moisture stress at which it would be unable to function if free-living. Four lichens were used: Lepraria membranacea Ach., Cladonia submitis Evans, Usnea substerilis Mot. and Letharia vulpina (L.) Hue, collected from diverse geographical locations. Moisture stress was quantified using the water potential concept, and water potential was controlled both osmotically and matrically. The function of the alga was evaluated by measuring photosynthesis with a (14)C technique. Photosynthesis of whole and ground lichen thalli was measured at different water potentials. Lowest water potentials permitting photosynthesis by whole lichen thalli ranged from-56 to-307 bar, and lowest potentials for the separated algal component ranged from-7 to-145 bar. In all cases the alga was able to photosynthesize at lower water potentials within the lichen thallus than when liberated. The grinding process did not harm the alga. It is suggested that one function of stored carbohydrate in the lichen fungus may be to increase internal osmotic pressure sufficiently so that the fungus thallus can absorb moisture from atmospheres of reduced humidity. If water secretion then occurred, some of this moisture could be made available to the alga.
    Language English
    Publishing date 2014-01-10
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 208909-9
    ISSN 1432-2048 ; 0032-0935 ; 1866-2749
    ISSN (online) 1432-2048
    ISSN 0032-0935 ; 1866-2749
    DOI 10.1007/BF00390063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Transcutaneous vagal nerve stimulation for treating gastrointestinal symptoms in individuals with diabetes: a randomised, double-blind, sham-controlled, multicentre trial.

    Kornum, Ditte S / Bertoli, Davide / Kufaishi, Huda / Wegeberg, Anne-Marie / Okdahl, Tina / Mark, Esben B / Høyer, Katrine L / Frøkjær, Jens B / Brock, Birgitte / Krogh, Klaus / Hansen, Christian S / Knop, Filip K / Brock, Christina / Drewes, Asbjørn M

    Diabetologia

    2024  

    Abstract: Aims/hypothesis: Diabetic gastroenteropathy frequently causes debilitating gastrointestinal symptoms. Previous uncontrolled studies have shown that transcutaneous vagal nerve stimulation (tVNS) may improve gastrointestinal symptoms. To investigate the ... ...

    Abstract Aims/hypothesis: Diabetic gastroenteropathy frequently causes debilitating gastrointestinal symptoms. Previous uncontrolled studies have shown that transcutaneous vagal nerve stimulation (tVNS) may improve gastrointestinal symptoms. To investigate the effect of cervical tVNS in individuals with diabetes suffering from autonomic neuropathy and gastrointestinal symptoms, we conducted a randomised, sham-controlled, double-blind (participants and investigators were blinded to the allocated treatment) study.
    Methods: This study included adults (aged 20-86) with type 1 or 2 diabetes, gastrointestinal symptoms and autonomic neuropathy recruited from three Steno Diabetes Centres in Denmark. Participants were randomly allocated 1:1 to receive active or sham stimulation. Active cervical tVNS or sham stimulation was self-administered over two successive study periods: 1 week of four daily stimulations and 8 weeks of two daily stimulations. The primary outcome measures were gastrointestinal symptom changes as measured using the gastroparesis cardinal symptom index (GCSI) and the gastrointestinal symptom rating scale (GSRS). Secondary outcomes included gastrointestinal transit times and cardiovascular autonomic function.
    Results: Sixty-eight participants were randomised to the active group, while 77 were randomised to the sham group. Sixty-three in the active and 68 in the sham group remained for analysis in study period 1, while 62 in each group were analysed in study period 2. In study period 1, active and sham tVNS resulted in similar symptom reductions (GCSI: -0.26 ± 0.64 vs -0.17 ± 0.62, p=0.44; GSRS: -0.35 ± 0.62 vs -0.32 ± 0.59, p=0.77; mean ± SD). In study period 2, active stimulation also caused a mean symptom decrease that was comparable to that observed after sham stimulation (GCSI: -0.47 ± 0.78 vs -0.33 ± 0.75, p=0.34; GSRS: -0.46 ± 0.90 vs -0.35 ± 0.79, p=0.50). Gastric emptying time was increased in the active group compared with sham (23 min vs -19 min, p=0.04). Segmental intestinal transit times and cardiovascular autonomic measurements did not differ between treatment groups (all p>0.05). The tVNS was well-tolerated.
    Conclusions/interpretation: Cervical tVNS, compared with sham stimulation, does not improve gastrointestinal symptoms among individuals with diabetes and autonomic neuropathy.
    Trial registration: ClinicalTrials.gov NCT04143269 FUNDING: The study was funded by the Novo Nordisk Foundation (grant number NNF180C0052045).
    Language English
    Publishing date 2024-03-28
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1694-9
    ISSN 1432-0428 ; 0012-186X
    ISSN (online) 1432-0428
    ISSN 0012-186X
    DOI 10.1007/s00125-024-06129-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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