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  1. Article ; Online: Estatus sanitario en pequeños sistemas productivos piscícolas de tilapia en el municipio de San Carlos, Antioquia

    Lina Correa Agudelo / Alejandro Ramírez Guerra / Janeth Pérez-García / Francisco José Arango Vacares

    Ciencia y Tecnología Agropecuaria, Vol 24, Iss

    2023  Volume 2

    Abstract: Las medidas de prevención y control sanitario en sistemas productivos piscícolas de tilapia son un reto para los pequeños productores campesinos en Colombia. El objetivo de este trabajo consistió en identificar in situ el estado sanitario de pequeños ... ...

    Abstract Las medidas de prevención y control sanitario en sistemas productivos piscícolas de tilapia son un reto para los pequeños productores campesinos en Colombia. El objetivo de este trabajo consistió en identificar in situ el estado sanitario de pequeños centros de producción de tilapia en el municipio de San Carlos, Antioquia. Se realizaron análisis macro y microscópico de 140 peces, se tomaron muestras de los principales órganos y se evaluó Streptococcus agalactiae, S. iniae, y Edwardsiella tarda a través de pcr. Se tomaron tres peces por producción y se determinó la presencia de Escherichia coli en filete. Además, se evaluó la concentración de oxígeno disuelto, la temperatura, los sólidos totales disueltos, la salinidad, la conductividad, el pH y la presencia microbiológica de E. coli y coliformes totales en agua. Por su parte, los hallazgos macroscópicos evidenciaron anormalidades en ojos, riñón posterior e hígado. Los microorganismos hallados fueron: monogéneos (53,9 %), tricodínidos (29,1 %) y Epistylis (7,8 %). Los peces con monogéneos presentaron un peso y una longitud mayor que aquellos negativos (p = 0,017 y 0,045, respectivamente) y lesiones en branquias en mayor proporción (rp = 1,56, ic95 % 1,17-2,06, p = 0,006). La presencia de tricodínidos presentó una relación estadísticamente significativa con el pH, la conductividad y la concentración de sal, además, las pruebas moleculares fueron negativas. La información generada puede utilizarse para plantear acciones zootécnicas y sanitarias para mejorar el estatus sanitario de los sistemas productivos de tilapia en San Carlos.
    Keywords calidad del agua ; inocuidad alimentaria ; Oreochromis ; producción de acuicultura ; sanidad ; Agriculture ; S ; Agriculture (General) ; S1-972 ; Animal culture ; SF1-1100
    Language Spanish
    Publishing date 2023-02-01T00:00:00Z
    Publisher Corporación Colombiana de Investigación Agropecuaria (Corpoica)
    Document type Article ; Online
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  2. Article ; Online: The Effects of Adding a Gel-Alike

    Guerra, Angélica Serpa / Hoyos, Catalina Gómez / Velásquez-Cock, Jorge / Vélez, Lina / Gañán, Piedad / Zuluaga, Robin

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 3

    Abstract: Color is an important characteristic of food products. This characteristic is related to consumer acceptability. To use the entire rhizome ... ...

    Abstract Color is an important characteristic of food products. This characteristic is related to consumer acceptability. To use the entire rhizome of
    MeSH term(s) Coloring Agents/pharmacology ; Curcuma/chemistry ; Food Handling/methods ; Humans ; Plant Extracts/pharmacology ; Rheology ; Sensation/drug effects ; Taste/drug effects ; Yogurt/analysis
    Chemical Substances Coloring Agents ; Plant Extracts ; turmeric extract (856YO1Z64F)
    Language English
    Publishing date 2022-01-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27030946
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  3. Article ; Online: Comparative clinical and molecular landscape of primary and secondary myelofibrosis: Superior performance of MIPSS70+ v2.0 over MYSEC-PM.

    Guerra, Matteo / Pasquer, Hélène / Daltro de Oliveira, Rafael / Soret-Dulphy, Juliette / Maslah, Nabih / Zhao, Lin-Pierre / Marcault, Clémence / Cazaux, Marine / Gauthier, Nicolas / Verger, Emmanuelle / Parquet, Nathalie / Vainchenker, William / Raffoux, Emmanuel / Giraudier, Stéphane / Cassinat, Bruno / Kiladjian, Jean-Jacques / Benajiba, Lina

    American journal of hematology

    2024  Volume 99, Issue 4, Page(s) 741–744

    Abstract: Comparative clinical characteristics, molecular landscape and prognosis scoring for primary (PMF) and secondary myelofibrosis (SMF). ...

    Abstract Comparative clinical characteristics, molecular landscape and prognosis scoring for primary (PMF) and secondary myelofibrosis (SMF).
    MeSH term(s) Humans ; Primary Myelofibrosis/genetics ; Prognosis
    Language English
    Publishing date 2024-01-26
    Publishing country United States
    Document type Letter
    ZDB-ID 196767-8
    ISSN 1096-8652 ; 0361-8609
    ISSN (online) 1096-8652
    ISSN 0361-8609
    DOI 10.1002/ajh.27226
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  4. Article: Characterization of a Novel Starch Isolated from the Rhizome of Colombian Turmeric (

    Argel-Pérez, Shaydier / Gañán-Rojo, Piedad / Cuartas-Marulanda, Diego / Gómez-Hoyos, Catalina / Velázquez-Cock, Jorge / Vélez-Acosta, Lina / Zuluaga, Robin / Serpa-Guerra, Angélica

    Foods (Basel, Switzerland)

    2023  Volume 13, Issue 1

    Abstract: Turmeric ( ...

    Abstract Turmeric (
    Language English
    Publishing date 2023-12-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods13010007
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  5. Article ; Online: A yeast-based high-throughput screen identifies inhibitors of trypanosomatid HRG heme transporters with potent leishmanicidal and trypanocidal activity.

    Cabello-Donayre, María / Cabello-Donayre, Irene / Guerra, Diego / Orrego, Lina M / Morales, Juan C / Cautain, Bastien / Vicente, Francisca / Pérez-Victoria, José M

    International journal of antimicrobial agents

    2024  Volume 63, Issue 3, Page(s) 107092

    Abstract: Objectives: New drugs are required to treat neglected diseases caused by trypanosomatid parasites such as Leishmania, Trypanosoma brucei and Trypanosoma cruzi. An Achilles' heel of these parasites is their heme auxotrophy; they have an absolute ... ...

    Abstract Objectives: New drugs are required to treat neglected diseases caused by trypanosomatid parasites such as Leishmania, Trypanosoma brucei and Trypanosoma cruzi. An Achilles' heel of these parasites is their heme auxotrophy; they have an absolute dependence on scavenging this molecule from the host, and trypanosomatid HRG heme transporters (TrypHRG) play an important role in this process. As these proteins are essential for the parasites and have low similarity with their human orthologue, they have been proposed as attractive therapeutic targets. Here, we have developed two yeast-based assays that allow an inexpensive high-throughput screening of TrypHRG inhibitors within a cellular context.
    Methods: We first assessed that Leishmania major, Leishmania donovani and T. brucei HRG proteins were heterologously expressed in the digestive vacuole membrane of a mutant heme auxotrophic yeast strain. Here, TrypHRG imports hemoglobinderived heme into the cytosol, allowing mutant yeast to grow in the presence of low hemoglobin concentrations and promoting the activity of hemeproteins such as catalase, which was used as a reporter of cytosolic heme levels.
    Results: In the presence of a TrypHRG inhibitor, both catalase activity (test 1) and yeast growth (test 2) were diminished, being easily monitored. The assays were then tested on a pilot scale for HTS purposes using a collection of repurposing drugs and food antioxidants. Some of the TrypHRG inhibitors identified in yeast presented strong trypanocidal and leishmanicidal activity in the submicromolar range, proving the potential of this approach.
    Conclusions: Cumulatively, it was shown that the inhibition bioassays developed were robust and applicable to large-scale HTS.
    MeSH term(s) Humans ; Saccharomyces cerevisiae/genetics ; Catalase ; Leishmania ; Biological Assay ; Heme
    Chemical Substances Catalase (EC 1.11.1.6) ; Heme (42VZT0U6YR)
    Language English
    Publishing date 2024-01-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1093977-5
    ISSN 1872-7913 ; 0924-8579
    ISSN (online) 1872-7913
    ISSN 0924-8579
    DOI 10.1016/j.ijantimicag.2024.107092
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    Zs.A 3368: Show issues Location:
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    Zs.MO 500: Show issues

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  6. Article ; Online: Apuntes sobre la competencia gestión personal del conocimiento microbiológico en la formación de profesores de biología

    Lina Aurora Campos-Martínez / Marisela de la Caridad Guerra-Salcedo / Yolexis Roberta Cardona-Soberao / Julio Cesar Rifa-Tellez

    Santiago (2023)

    2023  

    Abstract: En la actualidad, la formación de profesionales exige de una gestión del conocimiento que acompañe la autonomía de los estudiantes y la apropiación significativa y con sentido personal de saberes necesarios para enfrentar los desafíos y retos ... ...

    Abstract En la actualidad, la formación de profesionales exige de una gestión del conocimiento que acompañe la autonomía de los estudiantes y la apropiación significativa y con sentido personal de saberes necesarios para enfrentar los desafíos y retos profesionales que enfrentará al graduarse. De modo que, el objetivo del presente trabajo es la sistematización teórica de la competencia gestión personal del conocimiento microbiológico en la formación de profesores de biología, en las universidades cubanas. A partir del empleo de métodos teóricos, se conforman las bases teórico-metodológicas sobre esta competencia. Se profundiza en la aplicación de estrategias pedagógicas que favorezcan el proceso de gestión del conocimiento, la indagación y manejo de las variadas fuentes de información para convertirlo en conocimiento. Se ofrece una perspectiva diferente del valor que tiene esta competencia para las instituciones educativas del nivel superior, con respecto a la que posee a nivel de las organizaciones.
    Keywords formación del profesional ; competencias ; gestión personal del conocimiento ; microbiología ; French literature - Italian literature - Spanish literature - Portuguese literature ; PQ1-3999 ; Social Sciences ; H
    Language Spanish
    Publishing date 2023-03-01T00:00:00Z
    Publisher Universidad de Oriente
    Document type Article ; Online
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  7. Article ; Online: Spanish version of Multidimensional Mentalizing Questionnaire (MMQ): Translation, adaptation and psychometric properties in a Chilean population.

    Aldunate, Nerea / López-Silva, Pablo / Brotfeld, Cristian / Guerra, Ernesto / Kronmüller, Edmundo

    PloS one

    2024  Volume 19, Issue 3, Page(s) e0296691

    Abstract: ... Questionnaire (MMQ) into Spanish for a native Spanish-speaking sample in Chile. The study examines ... mentalization adapted to Chilean population, contributes to research aiming to understand its relationship ...

    Abstract This paper presents the first translation and adaptation of the Multidimensional Mentalizing Questionnaire (MMQ) into Spanish for a native Spanish-speaking sample in Chile. The study examines the psychometric properties and internal consistency of the translated MMQ. The instrument undergoes modifications based on a confirmatory factor analysis of the original structure, resulting in the elimination of items with cross-loadings and improvement in model fit. The modified scale is then analyzed, demonstrating strong psychometric properties. Convergent evidence is assessed by correlating MMQ subscales with the Interpersonal Reactivity Index (IRI) and Empathy Quotient (EQ), while divergent evidence is assessed by correlating aggressive traits using the Buss-Perry Aggression Questionnaire (BPAQ). The study also explores gender differences and age. Results reveal positive correlations between good mentalizing and empathy, particularly cognitive empathy, supporting the significance of positive mentalization in empathy. Negative mentalization is associated with difficulties in perspective-taking and social skills, as well as aggressive traits. Gender differences in mentalizing capacities are observed, and negative aspects of mentalization decrease with age. The availability of the Spanish translation of the MMQ, the first self-reporting scale measuring mentalization adapted to Chilean population, contributes to research aiming to understand its relationship with other psychological phenomena in different cultural context and facilitating clinical interventions with different population groups. We therefore encourage further investigation into cultural, gender and age differences in mentalization.
    MeSH term(s) Chile ; Mentalization ; Psychometrics ; Surveys and Questionnaires ; Empathy ; Reproducibility of Results
    Language English
    Publishing date 2024-03-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0296691
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  8. Article: Evaluation and validation of laboratory procedures for the surveillance of ESBL-, AmpC-, and carbapenemase-producing

    Hendriksen, Rene S / Cavaco, Lina M / Guerra, Beatriz / Bortolaia, Valeria / Agersø, Yvonne / Svendsen, Christina Aaby / Nielsen, Hanne Nørgaard / Kjeldgaard, Jette Sejer / Pedersen, Susanne Karlsmose / Fertner, Mette / Hasman, Henrik

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1229542

    Abstract: Introduction: Extended-spectrum β-lactamase- (ESBL) and AmpC- β-lactamase-producing : Methods: Up to nine laboratory procedures were tested using minced meat as the matrix from beef, pork, and chicken as well as six procedures for the caecal content ... ...

    Abstract Introduction: Extended-spectrum β-lactamase- (ESBL) and AmpC- β-lactamase-producing
    Methods: Up to nine laboratory procedures were tested using minced meat as the matrix from beef, pork, and chicken as well as six procedures for the caecal content of cattle, pigs, and chicken. Variables included sample volume, pre-enrichment volume, pre-enrichment broth with and without antimicrobial supplementation, and incubation time/temperature. The procedures were evaluated against up to nine
    Results and discussion: The laboratory procedures tested revealed that the most sensitive and specific methodologies were based on a Buffered Peptone Water pre-enrichment of 225 ml to 25 g or 9 ml to 1 g for minced meat and caecal content, respectively, incubated at 37°C overnight, followed by inoculation onto MacConkey agar supplemented with 1 mg/L cefotaxime for detecting ESBL- and AmpC-producing
    Language English
    Publishing date 2023-08-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1229542
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  9. Article ; Online: A Rare Case of Conjunctival Melanoma with Orbital Invasion.

    Guerra-Perez, Lina Maria / Bonilla-Escobar, Francisco J / Salamanca, Omar / Martínez-Blanco, Alexander / Alarcon-Arciniegas, Carolina

    Nepalese journal of ophthalmology : a biannual peer-reviewed academic journal of the Nepal Ophthalmic Society : NEPJOPH

    2021  Volume 12, Issue 24, Page(s) 352–354

    MeSH term(s) Conjunctival Neoplasms/diagnosis ; Humans ; Melanoma/diagnosis
    Language English
    Publishing date 2021-05-12
    Publishing country Nepal
    Document type Case Reports ; Letter
    ZDB-ID 2596436-7
    ISSN 2091-0320 ; 2091-0320
    ISSN (online) 2091-0320
    ISSN 2091-0320
    DOI 10.3126/nepjoph.v12i2.29461
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  10. Article ; Online: CEMIP (HYBID, KIAA1199): structure, function and expression in health and disease.

    Spataro, Sofia / Guerra, Concetta / Cavalli, Andrea / Sgrignani, Jacopo / Sleeman, Jonathan / Poulain, Lina / Boland, Andreas / Scapozza, Leonardo / Moll, Solange / Prunotto, Marco

    The FEBS journal

    2022  Volume 290, Issue 16, Page(s) 3946–3962

    Abstract: CEMIP (cell migration-inducing protein), also known as KIAA1199 or HYBID, is a protein involved in the depolymerisation of hyaluronic acid (HA), a major glycosaminoglycan component of the extracellular matrix. CEMIP was originally described in patients ... ...

    Abstract CEMIP (cell migration-inducing protein), also known as KIAA1199 or HYBID, is a protein involved in the depolymerisation of hyaluronic acid (HA), a major glycosaminoglycan component of the extracellular matrix. CEMIP was originally described in patients affected by nonsyndromic hearing loss and has subsequently been shown to play a key role in tumour initiation and progression, as well as arthritis, atherosclerosis and idiopathic pulmonary fibrosis. Despite the vast literature associating CEMIP with these diseases, its biology remains elusive. The present review article summarises all the major scientific evidence regarding its structure, function, role and expression, and attempts to cast light on a protein that modulates EMT, fibrosis and tissue inflammation, an unmet key aspect in several inflammatory disease conditions.
    MeSH term(s) Humans ; Cell Movement ; Extracellular Matrix/metabolism ; Hyaluronic Acid/metabolism ; Hyaluronoglucosaminidase/genetics ; Hyaluronoglucosaminidase/metabolism
    Chemical Substances Hyaluronic Acid (9004-61-9) ; Hyaluronoglucosaminidase (EC 3.2.1.35) ; CEMIP protein, human (EC 3.2.1.35)
    Language English
    Publishing date 2022-09-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2173655-8
    ISSN 1742-4658 ; 1742-464X
    ISSN (online) 1742-4658
    ISSN 1742-464X
    DOI 10.1111/febs.16600
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