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Article ; Online: SilE-R and SilE-S - DABB Proteins Catalyzing Enantiospecific Hydrolysis of Organosilyl Ethers.

Pick, Lisa M / Oehme, Viviane / Hartmann, Julia / Wenzlaff, Jessica / Tang, Qingyun / Grogan, Gideon / Ansorge-Schumacher, Marion B

Angewandte Chemie (International ed. in English)

2024 , Page(s) e202404105

Abstract: ... enzymes, SilE-R and SilE-S, which are able to hydrolyze silyl ethers. They belong to the stress-response ... silyl functions as large as TBDMS are accepted. The X-ray crystal structure of SilE-R, determined ... mechanism, which has not previously been described for enzymes. Although the natural function of SilE-R and ...

Abstract	Silyl ethers fulfil a fundamental role in synthetic organic chemistry as protecting groups and their selective cleavage is an important factor in their application. We present here for the first time two enzymes, SilE-R and SilE-S, which are able to hydrolyze silyl ethers. They belong to the stress-response A/B barrel domain (DABB) family and are able to cleave the Si-O bond with opposite enantiopreference. Silyl ethers containing aromatic, cyclic or aliphatic alcohols and, depending on the alcohol moiety, silyl functions as large as TBDMS are accepted. The X-ray crystal structure of SilE-R, determined to a resolution of 1.98 Ȧ, in combination with mutational studies, revealed an active site featuring two histidine residues, H8 and H79, which likely act synergistically as nucleophile and Brønsted base in the hydrolytic mechanism, which has not previously been described for enzymes. Although the natural function of SilE-R and SilE-S is unknown, we propose that these 'silyl etherases' may have significant potential for synthetic applications.
Language	English
Publishing date	2024-04-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.202404105
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book: Verbundprojekt R&D BESCHLEUNIGER (Spin Management): Spin-Management polarisierter Leptonstrahlen an Beschleunigern

Moortgat-Pick, Gudrid

Verbund: 05H2010 - R&D BESCHLEUNIGER (Spin Management) ; Schlussbericht ; Förderzeitraum: 01.07.2010 - 30.06.2012

2013

Title variant	R&D BESCHLEUNIGER (Spin Management): Spin-Management polarisierter Leptonstrahlen an Beschleunigern
Institution	Universität Hamburg
Author's details	Universität Hamburg ... Projektleitung: Gudrid Moortgat-Pick
Language	German
Size	23 Bl.
Publishing place	Hamburg
Document type	Book
Note	Förderkennzeichen BMBF 05H10GUE - 05H10PDE - 05H10UME. - Verbund-Nr. 01079160 ; Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Book ; Online: Verbundprojekt R&D BESCHLEUNIGER (Spin Management): Spin-Management polarisierter Leptonstrahlen an Beschleunigern

Moortgat-Pick, Gudrid

Verbund: 05H2010 - R&D BESCHLEUNIGER (Spin Management) ; Schlussbericht ; Förderzeitraum: 01.07.2010 - 30.06.2012

2013

Title variant	R&D BESCHLEUNIGER (Spin Management): Spin-Management polarisierter Leptonstrahlen an Beschleunigern
Institution	Universität Hamburg
Author's details	Universität Hamburg ... Projektleitung: Gudrid Moortgat-Pick
Language	German
Size	Online-Ressource (24 S., 207 KB)
Publisher	Technische Informationsbibliothek u. Universitätsbibliothek
Publishing place	Hannover ; Hamburg
Document type	Book ; Online
Note	Förderkennzeichen BMBF 05H10GUE - 05H10PDE - 05H10UME. - Verbund-Nr. 01079160 ; Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article: Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility.

Mor, I / Sklan, E H / Podoly, E / Pick, M / Kirschner, M / Yogev, L / Bar-Sheshet Itach, S / Schreiber, L / Geyer, B / Mor, T / Grisaru, D / Soreq, H

Journal of cellular and molecular medicine

2008 Volume 12, Issue 2, Page(s) 479–495

Abstract: ... of the acetylcholinesterase (AChE-R) splice variant, a common response in various tissues. In germ cells of male transgenic ... TgR mice, AChE-R excess associates with reduced sperm differentiation and sperm counts. To explore ... the mechanism(s) by which AChE-R up-regulation affects spermatogenesis, we identified AChE-R's protein partners ...

Abstract	Changes in protein subdomains through alternative splicing often modify protein-protein interactions, altering biological processes. A relevant example is that of the stress-induced up-regulation of the acetylcholinesterase (AChE-R) splice variant, a common response in various tissues. In germ cells of male transgenic TgR mice, AChE-R excess associates with reduced sperm differentiation and sperm counts. To explore the mechanism(s) by which AChE-R up-regulation affects spermatogenesis, we identified AChE-R's protein partners through a yeast two-hybrid screen. In meiotic spermatocytes from TgR mice, we detected AChE-R interaction with the scaffold protein RACK1 and elevated apoptosis. This correlated with reduced scavenging by RACK1 of the pro-apoptotic TAp73, an outcome compatible with the increased apoptosis. In contrast, at later stages in sperm development, AChE-R's interaction with the glycolytic enzyme enolase-alpha elevates enolase activity. In transfected cells, enforced AChE-R excess increased glucose uptake and adenosine tri-phosphate (ATP) levels. Correspondingly, TgR sperm cells display elevated ATP levels, mitochondrial hyperactivity and increased motility. In human donors' sperm, we found direct association of sperm motility with AChE-R expression. Interchanging interactions with RACK1 and enolase-alpha may hence enable AChE-R to affect both sperm differentiation and function by participating in independent cellular pathways.
MeSH term(s)	Acetylcholinesterase/genetics ; Acetylcholinesterase/metabolism ; Alternative Splicing ; Animals ; Apoptosis/genetics ; Biopsy ; Humans ; Immunohistochemistry ; Male ; Mice ; Mice, Transgenic ; Models, Biological ; Sperm Motility/genetics ; Spermatozoa/cytology ; Spermatozoa/enzymology ; Spermatozoa/physiology ; Testis/cytology ; Testis/enzymology ; Testis/metabolism ; Testis/physiology ; Testis/surgery
Chemical Substances	Acetylcholinesterase (EC 3.1.1.7)
Language	English
Publishing date	2008-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2074559-X
ISSN	1582-4934 ; 1582-1838 ; 1582-4934
ISSN (online)	1582-4934
ISSN	1582-1838 ; 1582-4934
DOI	10.1111/j.1582-4934.2008.00231.x
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Article: The stress-associated acetylcholinesterase variant AChE-R is expressed in human CD34(+) hematopoietic progenitors and its C-terminal peptide ARP promotes their proliferation.

Deutsch, Varda R / Pick, Marjorie / Perry, Chava / Grisaru, Dan / Hemo, Yoram / Golan-Hadari, Dita / Grant, Alastair / Eldor, Amiram / Soreq, Hermona

Experimental hematology

2003 Volume 30, Issue 10, Page(s) 1153–1161

Abstract: ... overexpression and alternative splicing, yielding the rare stress-associated "readthrough" AChE-R variant protein ... This led us to explore the hematopoietic involvement of AChE-R and its cleavable C-terminal peptide ARP ... Our findings support ARP, the C-terminal peptide of AChE-R, as a new hematopoietic growth factor ...

Abstract	Objective: Hematopoietic stress responses involve increases in leukocyte and platelet counts, implying the existence of stress responsive factors that modulate hematopoiesis. Acetylcholinesterase (AChE) is expressed in mammalian neurons and hematopoietic cells. In brain, it responds to stress by mRNA overexpression and alternative splicing, yielding the rare stress-associated "readthrough" AChE-R variant protein. This led us to explore the hematopoietic involvement of AChE-R and its cleavable C-terminal peptide ARP. Materials and methods: AChE mRNA variants were labeled in CD34(+) hematopoietic progenitor cells by in situ hybridization. ARP expression was detected by multicolor flow cytometry. Bromo-deoxyuracil incorporation and viable cell counts served to evaluate the proliferative effects of ARP and suppressive effects of the AChE antisense oligonucleotide AS1 on CD34(+) cells. Results: The distal enhancer, proximal promoter, and first intron of the human AChE gene include consensus binding sites for hematopoietically active and stress-induced transcription factors. CD34(+) cells from human cord blood were found to express all three variant AChE mRNAs, having different intracellular distributions. ARP was found in 5 to 15% of adult peripheral blood, bone marrow, and fetal CD34(+) cells (both committed CD38(+) and uncommitted CD38(-)) and in acute myeloid leukemia blasts. Externally supplied ARP by itself facilitated the proliferation of CD34(+) cells in an antisense suppressible manner. When combined with early-acting cytokines, ARP enhanced survival and expansion of CD34(+) cells up to 28 days in culture. Conclusions: Our findings support ARP, the C-terminal peptide of AChE-R, as a new hematopoietic growth factor that may promote the myelopoietic expansion and thrombopoiesis characteristic of stress and may be used to enhance the efficiency of ex vivo expansion for bone marrow transplantation.
MeSH term(s)	Acetylcholinesterase/genetics ; Adult ; Antigens, CD/blood ; Antigens, CD34/blood ; Cell Division/physiology ; Fetal Blood/cytology ; Fetus/physiology ; Flow Cytometry ; Genetic Variation ; Growth Substances/pharmacology ; Hematopoietic Stem Cells/cytology ; Hematopoietic Stem Cells/enzymology ; Humans ; Immunophenotyping ; In Situ Hybridization ; Infant, Newborn ; Oligodeoxyribonucleotides, Antisense ; Peptide Fragments/pharmacology ; Stress, Physiological/enzymology ; Stress, Physiological/genetics
Chemical Substances	Antigens, CD ; Antigens, CD34 ; Growth Substances ; Oligodeoxyribonucleotides, Antisense ; Peptide Fragments ; Acetylcholinesterase (EC 3.1.1.7)
Language	English
Publishing date	2003-04-01
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	185107-x
ISSN	1873-2399 ; 0301-472X ; 0531-5573
ISSN (online)	1873-2399
ISSN	0301-472X ; 0531-5573
DOI	10.1016/s0301-472x(02)00900-1
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Article: Kinetics of the thapsigargin-induced Ca

Pick, Tillman / Gamayun, Igor / Tinschert, René / Cavalié, Adolfo

Frontiers in physiology

2023 Volume 14, Page(s) 1127545

Abstract: Thapsigargin (TG) inhibits the sarco/endoplasmic reticulum ... ...

Abstract	Thapsigargin (TG) inhibits the sarco/endoplasmic reticulum Ca
Language	English
Publishing date	2023-03-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2023.1127545
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Article ; Online: Studying TIGIT activity against tumors through the generation of knockout mice.

Rishiq, Ahmed / Bsoul, Reem / Pick, Ophir / Mandelboim, Ofer

Oncoimmunology

2023 Volume 12, Issue 1, Page(s) 2217735

Abstract: The use of antibodies to block inhibitory receptors, primarily anti-PD1 and CTLA4 (known as checkpoint therapy) revolutionized cancer treatment. However, despite these successes, the majority of cancer patients do not respond to the checkpoint treatment, ...

Abstract	The use of antibodies to block inhibitory receptors, primarily anti-PD1 and CTLA4 (known as checkpoint therapy) revolutionized cancer treatment. However, despite these successes, the majority of cancer patients do not respond to the checkpoint treatment, emphasizing the need for development of additional therapies, which are based on other inhibitory receptors. Human TIGIT is an inhibitory receptor expressed by Natural Killer (NK) and T cells and is mainly known to interact with PVR, Nectin-2, Nectin-3, and Nectin-4. Whether mouse TIGIT interacts with all of these ligands is still unclear. Additionally, the in vivo function of TIGIT against tumors is not completely understood. Here, we demonstrate that mouse TIGIT interacts with and is inhibited by mPVR only. Using CRISPR-Cas9 technology, we generated TIGIT-deficient mice and demonstrated that NK cell cytotoxicity and degranulation against two tumor types were lower in WT mice when compared to the TIGIT KO mice. Moreover, in vivo tumor progression was slower in TIGIT KO than in WT mice. Taken together, our data established that mTIGIT has only one ligand, PVR, and that in the absence of TIGIT tumors are killed better both in vitro and in vivo.
MeSH term(s)	Animals ; Humans ; Mice ; Cell Adhesion Molecules/genetics ; Ligands ; Mice, Knockout ; Neoplasms/genetics ; Receptors, Immunologic/genetics ; Receptors, Immunologic/metabolism ; T-Lymphocytes
Chemical Substances	Cell Adhesion Molecules ; Ligands ; Receptors, Immunologic ; TIGIT protein, human ; T cell Ig and ITIM domain protein, mouse
Language	English
Publishing date	2023-05-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2645309-5
ISSN	2162-402X ; 2162-402X
ISSN (online)	2162-402X
ISSN	2162-402X
DOI	10.1080/2162402X.2023.2217735
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Article: Connectivity of stormwater ponds impacts Odonata abundance and species richness.

Richmond, I C / Perron, M C / Boyle, S P / Pick, F R

Landscape ecology

2024 Volume 39, Issue 3, Page(s) 63

Abstract: ... species richness, and composition of dragonflies (p values < 0.01, R: Conclusions: Our results suggest ...

Abstract	Context: The successful dispersal of an animal depends, partly, on landscape connectivity. Urbanization poses risks to dispersal activities by increasing hostile land cover types. Objectives: We investigated how connectivity of urban ponds impacted Odonata communities (dragonflies and damselflies), an order of semi-aquatic insects that actively disperse. Methods: We sampled 41 constructed stormwater ponds and 8 natural ponds in a metropolitan area. The effect of connectivity and the quantity of available adjacent habitats was tested at different scales for dragonflies (900 m) and damselflies (300 m), determined by a literature analysis, to account for differences in suborder dispersal capabilities. Results: Lower levels of connectivity and fewer nearest neighbours negatively impacted abundance, species richness, and composition of dragonflies (p values < 0.01, R Conclusions: Our results suggest that dragonflies are positively affected by increased connectivity in an urban landscape, with no benefit of connectivity to damselflies at the scale measured. We recommend intentional planning of urban stormwater pond networks, where individual ponds can act as stepping stones, incorporated with strategic inclusion of beneficial land cover types. Supplementary information: The online version contains supplementary material available at 10.1007/s10980-024-01817-z.
Language	English
Publishing date	2024-02-28
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1027798-5
ISSN	1572-9761 ; 0921-2973
ISSN (online)	1572-9761
ISSN	0921-2973
DOI	10.1007/s10980-024-01817-z
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Article ; Online: Advice for improving the reproducibility of data extraction in meta-analysis.

Ivimey-Cook, Edward R / Noble, Daniel W A / Nakagawa, Shinichi / Lajeunesse, Marc J / Pick, Joel L

Research synthesis methods

2023 Volume 14, Issue 6, Page(s) 911–915

Abstract: ... in meta-analysis. We also provide suggestions of R software that can further help with reproducible data policies ...

Abstract	Extracting data from studies is the norm in meta-analyses, enabling researchers to generate effect sizes when raw data are otherwise not available. While there has been a general push for increased reproducibility in meta-analysis, the transparency and reproducibility of the data extraction phase is still lagging behind. Unfortunately, there is little guidance of how to make this process more transparent and shareable. To address this, we provide several steps to help increase the reproducibility of data extraction in meta-analysis. We also provide suggestions of R software that can further help with reproducible data policies: the shinyDigitise and juicr packages. Adopting the guiding principles listed here and using the appropriate software will provide a more transparent form of data extraction in meta-analyses.
MeSH term(s)	Reproducibility of Results ; Software
Language	English
Publishing date	2023-08-11
Publishing country	England
Document type	Meta-Analysis ; Journal Article
ZDB-ID	2548499-0
ISSN	1759-2887 ; 1759-2879
ISSN (online)	1759-2887
ISSN	1759-2879
DOI	10.1002/jrsm.1663
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Article ; Online: Mental health and neurodevelopmental patient-reported outcome measures (PROMs) for children and young people with epilepsy: A systematic review.

De Aveiro, Bianca / Winsor, Alice / Davies, Jessica / Nicholson, Timothy R / Pal, Deb K / Richardson, Mark P / Pick, Susannah

Epilepsy & behavior : E&B

2024 Volume 153, Page(s) 109671

Abstract: Children and young people with epilepsy are at higher risk of mental health disorders and atypical neurodevelopmental outcomes compared to the general population. It is essential to detect such comorbidities early in children with epilepsy and provide ... ...

Abstract	Children and young people with epilepsy are at higher risk of mental health disorders and atypical neurodevelopmental outcomes compared to the general population. It is essential to detect such comorbidities early in children with epilepsy and provide appropriate interventions, to improve clinical outcomes. We aimed to identify and evaluate the measurement properties of Patient-Reported Outcome Measures (PROMs) that have been validated specifically to measure mental health and neurodevelopmental outcomes in children and/or young people with epilepsy. We searched Embase, Medline, and PsycINFO in May 2023 for relevant studies. Mental health was defined as psychological symptoms (e.g., anxiety, depression, psychosis) and/or behavioural difficulties (e.g., conduct disorders). Neurodevelopmental outcomes included neurodevelopmental disorder traits such as attention-deficit hyperactivity disorder (ADHD) and autistic spectrum disorders. We assessed methodological quality using Consensus-based Standards for the selection of health Measurement Instruments (COSMIN) guidance. Twelve papers were identified that psychometrically evaluated 13 relevant PROMs (two epilepsy-specific, eleven generic). The appraisal of the PROMs was limited by the availability of only one or two published articles for each, and incomplete psychometric evaluations in some cases. The tool demonstrating the strongest evidence was The Neurological Disorders Depression Inventory-Epilepsy for Youth. The ADHD Rating Scale-IV and The Paediatric Symptom Checklist -17 demonstrated good evidence in favour of at least two measurement properties. This review identified only a small number of mental health and neurodevelopmental PROMs evaluated specifically in paediatric epilepsy. There is a need for further validation of mental health and neurodevelopmental PROMs in children with epilepsy.
MeSH term(s)	Adolescent ; Humans ; Child ; Mental Health ; Psychotic Disorders ; Epilepsy/complications ; Epilepsy/therapy ; Anxiety Disorders ; Patient Reported Outcome Measures ; Quality of Life/psychology
Language	English
Publishing date	2024-02-19
Publishing country	United States
Document type	Systematic Review ; Journal Article ; Review
ZDB-ID	2010587-3
ISSN	1525-5069 ; 1525-5050
ISSN (online)	1525-5069
ISSN	1525-5050
DOI	10.1016/j.yebeh.2024.109671
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