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  1. Book ; Thesis: Engineering of polyketide production routes in the amoeba Dictyostelium discoideum

    Reimer, Christin / Brakhage, Axel. A. / Winckler, Thomas / Nett, Markus

    2022  

    Institution Friedrich-Schiller-Universität Jena
    Author's details by Christin Reimer (M.Sc.)
    Language English ; German
    Size 132, IX Blätter, Illustrationen, Diagramme, 30 cm
    Publishing place Jena
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dissertation, Friedrich-Schiller-Universität Jena, 2022
    Note Zusammenfassungen in deutscher und englischer Sprache ; Zeitschriftenaufsätze in englischer Sprache ; Kumulative Dissertation, enthält Zeitschriftenaufsätze. - Tag der Verteidigung: 01.12.2022
    HBZ-ID HT021733273
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2023 E 369 order for loan Magazin

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  2. Article: Aurachins, Bacterial Antibiotics Interfering with Electron Transport Processes.

    Kruth, Sebastian / Nett, Markus

    Antibiotics (Basel, Switzerland)

    2023  Volume 12, Issue 6

    Abstract: Aurachins are farnesylated quinolone alkaloids of bacterial origin and excellent inhibitors of the respiratory chain in pro- and eukaryotes. Therefore, they have become important tool compounds for the investigation of electron transport processes and ... ...

    Abstract Aurachins are farnesylated quinolone alkaloids of bacterial origin and excellent inhibitors of the respiratory chain in pro- and eukaryotes. Therefore, they have become important tool compounds for the investigation of electron transport processes and they also serve as lead structures for the development of antibacterial and antiprotozoal drugs. Especially aurachin D proved to be a valuable starting point for structure-activity relationship studies. Aurachin D is a selective inhibitor of the cytochrome
    Language English
    Publishing date 2023-06-17
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics12061067
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  3. Article: Intermediates and shunt products of massiliachelin biosynthesis in

    Steinmetz, Till / Lombe, Blaise Kimbadi / Nett, Markus

    Beilstein journal of organic chemistry

    2023  Volume 19, Page(s) 909–917

    Abstract: Siderophores are small molecules secreted by microorganisms in order to scavenge iron from the environment. An example is the thiazoline-containing natural product massiliachelin, which is produced ... ...

    Abstract Siderophores are small molecules secreted by microorganisms in order to scavenge iron from the environment. An example is the thiazoline-containing natural product massiliachelin, which is produced by
    Language English
    Publishing date 2023-06-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2192461-2
    ISSN 1860-5397
    ISSN 1860-5397
    DOI 10.3762/bjoc.19.69
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  4. Article: Amamistatins isolated from

    Steinmetz, Till / Hiller, Wolf / Nett, Markus

    Beilstein journal of organic chemistry

    2022  Volume 18, Page(s) 360–367

    Abstract: Four new phenolic siderophores were isolated from the ... ...

    Abstract Four new phenolic siderophores were isolated from the actinomycete
    Language English
    Publishing date 2022-03-30
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2192461-2
    ISSN 1860-5397
    ISSN 1860-5397
    DOI 10.3762/bjoc.18.40
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  5. Article ; Online: Saccharomyces cerevisiae as host for the recombinant production of polyketides and nonribosomal peptides.

    Tippelt, Anna / Nett, Markus

    Microbial cell factories

    2021  Volume 20, Issue 1, Page(s) 161

    Abstract: As a robust, fast growing and genetically tractable organism, the budding yeast Saccharomyces cerevisiae is one of the most widely used hosts in biotechnology. Its applications range from the manufacturing of vaccines and hormones to bulk chemicals and ... ...

    Abstract As a robust, fast growing and genetically tractable organism, the budding yeast Saccharomyces cerevisiae is one of the most widely used hosts in biotechnology. Its applications range from the manufacturing of vaccines and hormones to bulk chemicals and biofuels. In recent years, major efforts have been undertaken to expand this portfolio to include structurally complex natural products, such as polyketides and nonribosomally synthesized peptides. These compounds often have useful pharmacological properties, which make them valuable drugs for the treatment of infectious diseases, cancer, or autoimmune disorders. In nature, polyketides and nonribosomal peptides are generated by consecutive condensation reactions of short chain acyl-CoAs or amino acids, respectively, with the substrates and reaction intermediates being bound to large, multidomain enzymes. For the reconstitution of these multistep catalytic processes, the enzymatic assembly lines need to be functionally expressed and the required substrates must be supplied in reasonable quantities. Furthermore, the production hosts need to be protected from the toxicity of the biosynthetic products. In this review, we will summarize and evaluate the status quo regarding the heterologous production of polyketides and nonribosomal peptides in S. cerevisiae. Based on a comprehensive literature analysis, prerequisites for a successful pathway reconstitution could be deduced, as well as recurring bottlenecks in this microbial host.
    MeSH term(s) Biological Products/metabolism ; Metabolic Networks and Pathways ; Peptide Biosynthesis ; Peptides/chemistry ; Peptides/metabolism ; Polyketide Synthases/metabolism ; Polyketides/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism
    Chemical Substances Biological Products ; Peptides ; Polyketides ; Polyketide Synthases (79956-01-7)
    Language English
    Publishing date 2021-08-19
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-021-01650-y
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  6. Article ; Online: Intermediates and shunt products of massiliachelin biosynthesis in Massilia sp. NR 4-1

    Till Steinmetz / Blaise Kimbadi Lombe / Markus Nett

    Beilstein Journal of Organic Chemistry, Vol 19, Iss 1, Pp 909-

    2023  Volume 917

    Abstract: Siderophores are small molecules secreted by microorganisms in order to scavenge iron from the environment. An example is the thiazoline-containing natural product massiliachelin, which is produced by Massilia sp. NR 4-1 under iron-deficient conditions. ... ...

    Abstract Siderophores are small molecules secreted by microorganisms in order to scavenge iron from the environment. An example is the thiazoline-containing natural product massiliachelin, which is produced by Massilia sp. NR 4-1 under iron-deficient conditions. Based on experimental evidence and genome analysis, it was suspected that this bacterium synthesizes further iron-chelating molecules. After a thorough inspection of its metabolic profile, six previously overlooked compounds were isolated that were active in the chrome azurol S (CAS) assay. Mass spectrometric measurements and nuclear magnetic resonance spectroscopic analyses identified these compounds as possible biosynthetic intermediates or shunt products of massiliachelin. Their bioactivity was tested against one Gram-positive and three Gram-negative bacteria.
    Keywords massilia ; massiliachelin ; siderophore ; structure elucidation ; Science ; Q ; Organic chemistry ; QD241-441
    Subject code 540
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Beilstein-Institut
    Document type Article ; Online
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  7. Article ; Online: Emerging concepts in the semisynthetic and mutasynthetic production of natural products.

    Vollmann, Dustin J / Winand, Lea / Nett, Markus

    Current opinion in biotechnology

    2022  Volume 77, Page(s) 102761

    Abstract: Natural products have greatly influenced the development of drugs to combat infectious diseases, cancer, and other disorders affecting human well-being. Only rarely, a natural product is used in an unmodified form for therapeutic purposes. More often, ... ...

    Abstract Natural products have greatly influenced the development of drugs to combat infectious diseases, cancer, and other disorders affecting human well-being. Only rarely, a natural product is used in an unmodified form for therapeutic purposes. More often, natural product derivatives are preferred due to improved activity or toxicity profiles. These compounds are usually produced using 'hybrid' processes that integrate organic synthesis and biosynthesis. Either a natural product is isolated from a biological source and then converted into the final drug by semisynthesis or a synthetically prepared precursor is introduced into the engineered biosynthesis of a living cell in a procedure called mutasynthesis. In this review, we will present recent developments in these two research areas, which take advantage of heterologous biosynthesis.
    MeSH term(s) Biological Products ; Humans
    Chemical Substances Biological Products
    Language English
    Publishing date 2022-07-30
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2022.102761
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3071: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: Biocatalytic production of the antibiotic aurachin D in Escherichia coli.

    Kruth, Sebastian / Schibajew, Lina / Nett, Markus

    AMB Express

    2022  Volume 12, Issue 1, Page(s) 138

    Abstract: Aurachin D is a potent inhibitor of cytochrome bd oxidases, which are potential targets in the treatment of infectious diseases. In this study, our aim was to improve the biocatalytic production of aurachin D from a quinolone precursor molecule with ... ...

    Abstract Aurachin D is a potent inhibitor of cytochrome bd oxidases, which are potential targets in the treatment of infectious diseases. In this study, our aim was to improve the biocatalytic production of aurachin D from a quinolone precursor molecule with recombinant Escherichia coli cells expressing the biosynthesis enzyme AuaA. In order to achieve a high-level production of this membrane-bound farnesyltransferase in E. coli, the expression of the auaA gene was translationally coupled to an upstream cistron in accordance with a bicistronic design (BCD) strategy. Screening of various BCD elements led to the identification of optimized auaA expression cassettes, which increased the aurachin D titer in E. coli up to 29-fold in comparison to T7-mediated expression. This titer could be further raised by codon optimization of auaA and by introducing the mevalonate pathway into the production strain. The latter measure was intended to improve the availability of farnesyl pyrophosphate, which is needed as a cosubstrate for the AuaA-catalyzed reaction. In sum, the described efforts resulted in a strain producing aurachin D with a titer that is 424 times higher than that obtained with the original, non-optimized expression host.
    Language English
    Publishing date 2022-11-03
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2621432-5
    ISSN 2191-0855
    ISSN 2191-0855
    DOI 10.1186/s13568-022-01478-8
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  9. Article ; Online: Amamistatins isolated from Nocardia altamirensis

    Till Steinmetz / Wolf Hiller / Markus Nett

    Beilstein Journal of Organic Chemistry, Vol 18, Iss 1, Pp 360-

    2022  Volume 367

    Abstract: Four new phenolic siderophores were isolated from the actinomycete Nocardia altamirensis along with the known natural product amamistatin B and a putative biosynthetic shunt product. The structures of all compounds were elucidated through 1D and 2D NMR ... ...

    Abstract Four new phenolic siderophores were isolated from the actinomycete Nocardia altamirensis along with the known natural product amamistatin B and a putative biosynthetic shunt product. The structures of all compounds were elucidated through 1D and 2D NMR analyses as well as mass spectrometry. The iron-chelating properties of the retrieved metabolites were evaluated in a chrome azurol S assay.
    Keywords actinomycete ; amamistatin ; nocardia ; siderophore ; structure elucidation ; Science ; Q ; Organic chemistry ; QD241-441
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Beilstein-Institut
    Document type Article ; Online
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  10. Book ; Online ; Thesis: Expanding the toolbox for engineering nonribosomal peptide synthetases

    Pourmasoumi, Farzaneh [Verfasser] / Hertweck, Christian [Gutachter] / Rosenbaum, Miriam [Gutachter] / Nett, Markus [Gutachter]

    2023  

    Author's details Farzaneh Pourmasoumi ; Gutachter: Christian Hertweck, Miriam Rosenbaum, Markus Nett
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Friedrich-Schiller-Universität Jena
    Publishing place Jena
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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