LIVIVO - Search results -

Search results

Result 1 - 10 of total 184

Search options

Article: Editorial: Drug resistance in breast cancer - mechanisms and approaches to overcome chemoresistance.

Raman, Dayanidhi / Cimpean, Anca Maria / De Miglio, Maria Rosaria

2023 Volume 12, Page(s) 1080684

Language	English
Publishing date	2023-01-04
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2022.1080684
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Artificial Intelligence (AI) Based Analysis of

Salvante, Enrica Raffaella Grazia / Popoiu, Anca Voichita / Barb, Alina Cristina / Cosma, Andrei Alexandru / Fenesan, Mihaela Pasca / Saxena, Amulya K / Popoiu, Tudor Alexandru / Boia, Eugen Sorin / Stanciulescu, Maria Corina / Caplar, Borislav Dusan / Dorobantu, Florica Ramona / Cimpean, Anca Maria

In vivo (Athens, Greece)

2024 Volume 38, Issue 2, Page(s) 620–629

Abstract: Background/aim: Biomaterials are essential in modern medicine, both for patients and research. Their ability to acquire and maintain functional vascularization is currently debated. The aim of this study was to evaluate the vascularization induced by ... ...

Abstract	Background/aim: Biomaterials are essential in modern medicine, both for patients and research. Their ability to acquire and maintain functional vascularization is currently debated. The aim of this study was to evaluate the vascularization induced by two collagen-based scaffolds (with 2D and 3D structures) and one non-collagen scaffold implanted on the chick embryo chorioallantoic membrane (CAM). Materials and methods: Classical stereomicroscopic image vascular assessment was enhanced with the IKOSA software by using two applications: the CAM assay and the Network Formation Assay, evaluating the vessel branching potential, vascular area, as well as tube length and thickness. Results: Both collagen-based scaffolds induced non-inflammatory angiogenesis, but the non-collagen scaffold induced a massive inflammation followed by inflammatory-related angiogenesis. Vessels branching points/Region of Interest (Px^2) and Vessel branching points/Vessel total area (Px^2), increased exponentially until day 5 of the experiment certifying a sustained and continuous angiogenic process induced by 3D collagen scaffolds. Conclusion: Collagen-based scaffolds may be more suitable for neovascularization compared to non-collagen scaffolds. The present study demonstrates the potential of the CAM model in combination with AI-based software for the evaluation of vascularization in biomaterials. This approach could help to reduce and replace animal experimentation in the pre-screening of biomaterials.
MeSH term(s)	Animals ; Chick Embryo ; Humans ; Tissue Scaffolds/chemistry ; Polymers ; Artificial Intelligence ; Neovascularization, Physiologic ; Biocompatible Materials/pharmacology ; Collagen/pharmacology ; Collagen/chemistry ; Neovascularization, Pathologic ; Tissue Engineering
Chemical Substances	Polymers ; Biocompatible Materials ; Collagen (9007-34-5)
Language	English
Publishing date	2024-02-27
Publishing country	Greece
Document type	Journal Article
ZDB-ID	807031-3
ISSN	1791-7549 ; 0258-851X
ISSN (online)	1791-7549
ISSN	0258-851X
DOI	10.21873/invivo.13481
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2288: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Historical Overview of In Vivo and In Vitro Angiogenesis Assays.

Cimpean, Anca Maria / Raica, Marius

Methods in molecular biology (Clifton, N.J.)

2019 Volume 2206, Page(s) 1–13

Abstract: Most of angiogenesis assays were designed and developed during Folkman's era. But the growth of new blood vessels in several pathologic conditions as tumor development or inflammation were observed long time ago.The development of new blood vessels was ... ...

Abstract	Most of angiogenesis assays were designed and developed during Folkman's era. But the growth of new blood vessels in several pathologic conditions as tumor development or inflammation were observed long time ago.The development of new blood vessels was early observed by ancient Egyptians who tried to destroy them by applying empirical methods. From the first observations regarding angiogenesis to a personalized therapy targeting newly formed blood vessels a lot of experimental in vitro and in vivo angiogenesis assays have been developed. The present work will overview the oldest and less known part of angiogenesis assays development, and in addition, it will present the newest data in the experimental field of angiogenesis which is rapidly improved by the needs of new antiangiogenic and antivascular therapy development.
MeSH term(s)	Angiogenesis Inhibitors/pharmacology ; Angiogenesis Inhibitors/therapeutic use ; Animals ; Biological Assay/methods ; Blood Vessels/drug effects ; Blood Vessels/pathology ; Humans ; Neovascularization, Pathologic/drug therapy ; Neovascularization, Pathologic/pathology
Chemical Substances	Angiogenesis Inhibitors
Language	English
Publishing date	2019-05-09
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-0916-3_1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Tumour-associated Angiogenesis and Intermediate Blood Vessels in Renal Cell Carcinoma.

Raica, Marius / Cimpean, Anca Maria / Ferician, Ovidiu Catalin / Ferician, Adela Maria

Cancer diagnosis & prognosis

2021 Volume 1, Issue 3, Page(s) 231–234

Abstract: Background/aim: Renal cell carcinoma is strongly vascularized, and formation of new blood vessels is a complex and multi-step process. In this study, we analysed the subtypes of intermediate blood vessels, as shown by double immunohistochemistry.: ... ...

Abstract	Background/aim: Renal cell carcinoma is strongly vascularized, and formation of new blood vessels is a complex and multi-step process. In this study, we analysed the subtypes of intermediate blood vessels, as shown by double immunohistochemistry. Materials and methods: Tumour-associated blood vessels were identified by double immunostaining based on CD34 and smooth muscle cell actin. Blood vessels were classified both quantitatively and qualitatively based on the expression of the aforementioned two markers. The main criteria to sub-classify intermediate blood vessels was the presence, distribution, and arrangement of perivascular cells. Results: We described three subtypes of intermediate blood vessels found particularly in the tumour area: Subtype 1 lacked perivascular cells, subtype 2 showed scattered pericytes attached to the vascular wall, and subtype 3 showed a continuous layer of perivascular cells on one side. Conclusion: We describe for the first time three subtypes of renal cell carcinoma-associated intermediate blood vessels, which could be important in prognosis and as potential targets for anti-vascular therapy.
Language	English
Publishing date	2021-07-03
Publishing country	Greece
Document type	Journal Article
ISSN	2732-7787
ISSN (online)	2732-7787
DOI	10.21873/cdp.10031
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article: VEGF Pathway Gene Expression Profile of Proliferating versus Involuting Infantile Hemangiomas: Preliminary Evidence and Review of the Literature.

Heredea, Rodica Elena / Melnic, Eugen / Cirligeriu, Laura Elena / Berzava, Patricia Lorena / Stănciulescu, Maria Corina / Popoiu, Călin Marius / Cimpean, Anca Maria

Children (Basel, Switzerland)

2022 Volume 9, Issue 6

Abstract: ... ...

Abstract	Background
Language	English
Publishing date	2022-06-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2732685-8
ISSN	2227-9067
ISSN	2227-9067
DOI	10.3390/children9060908
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Patterns of Tumor Vasculogenesis on a Chick Embryo Chorioallantoic Membrane

Comsa, Serban / Ceausu, Amalia-Raluca / Popescu, Roxana / Cimpean, Anca-Maria / Sarb, Simona / Raica, Marius

Anticancer research

2022 Volume 42, Issue 2, Page(s) 877–883

Abstract: Background/aim: Understanding tumor vasculogenesis is a cornerstone for the inhibition of tumor progression. This study aimed to generate an in vivo breast cancer environment to analyze the patterns of tumor vasculogenesis.: Materials and methods: ... ...

Abstract	Background/aim: Understanding tumor vasculogenesis is a cornerstone for the inhibition of tumor progression. This study aimed to generate an in vivo breast cancer environment to analyze the patterns of tumor vasculogenesis. Materials and methods: Human mesenchymal stem cells (hMSC) and breast cancer MCF-7 cells (MCF-7) were seeded onto a chorioallantoic membrane (CAM) and, after a 7-day incubation, we performed a morphological and immunohistochemical analysis of CAM. Results: hMSC and MCF-7 activated vasculogenesis and hematopoiesis on CAM. They stimulated the development of cord/capillary-like structures (CLS), formed by endothelial-like cells and hematopoietic cells. CLS presented a polygonal pattern, evolving towards a clearly visible plexus. Immunohistochemically, CLS were CD105+/AC133+/Oct3/4+, and the intensity was weak-moderate in the endothelial-like cells (inconstant) and weak in the hematopoietic cells. Conclusion: Tumor and embryonic vasculogenesis share a common paradigm, while CD105, AC133, and Oct3/4 were found to play a role in establishing the vasculogenic and hematopoietic stage.
MeSH term(s)	AC133 Antigen/metabolism ; Animals ; Breast Neoplasms/blood supply ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Chick Embryo ; Chorioallantoic Membrane/metabolism ; Chorioallantoic Membrane/pathology ; Disease Models, Animal ; Endoglin/metabolism ; Female ; Humans ; MCF-7 Cells ; Mesenchymal Stem Cells ; Neovascularization, Pathologic/metabolism ; Neovascularization, Pathologic/pathology ; Octamer Transcription Factor-3/metabolism
Chemical Substances	AC133 Antigen ; Endoglin ; Octamer Transcription Factor-3
Language	English
Publishing date	2022-01-29
Publishing country	Greece
Document type	Journal Article
ZDB-ID	604549-2
ISSN	1791-7530 ; 0250-7005
ISSN (online)	1791-7530
ISSN	0250-7005
DOI	10.21873/anticanres.15545
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1642: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Validation of Human Bone Marrow-derived Mesenchymal Stem Cells and MCF-7 Breast Cancer Cells Co-culture Using a 3D Perfused Biomimetic Microfluidic Platform.

Cimpean, Anca Maria / Comsa, Serban / Sturza, Adrian / Barb, Alina Cristina / Cosma, Andrei Alexandru / Fenesan, Mihaela Pasca / Ionescu, Corneliu / Ile, Alice Maria / Sarb, Simona / Chis, Monica

Anticancer research

2024 Volume 44, Issue 4, Page(s) 1441–1453

Abstract: Background/aim: Microfluidic experimental models allow to study the mutual interrelation between tumor development and the microvasculature avoiding animal use and lacking interspecies differences. This study aimed to develop and characterize a 3D ... ...

Abstract	Background/aim: Microfluidic experimental models allow to study the mutual interrelation between tumor development and the microvasculature avoiding animal use and lacking interspecies differences. This study aimed to develop and characterize a 3D tissue culture model employing a two-compartment microfluidic chip-perfused platform to visualize and quantify human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and MCF-7 breast cancer cell-cell interactions in real time. Materials and methods: MCF-7 cells were implanted in the tumor chamber and hBM-MSCs were injected into microvascular channels. hBM-MSCs culture media was perfused into microvascular compartments. The microfluidic device was microscopically examined weekly for four weeks. Results: VE- and E-cadherin immunofluorescence validated hBM-MSCs differentiation into endothelial cells and MCF-7 cell tumor formation. hBM-MSCs differentiation was highly heterogeneous along the microvascular channels, due to different perfusion flow. hBM-MSCs lining microvascular channels acquired VE-cadherin positive endothelial phenotype and continuously covered microchannels as an endothelium like layer. MCF-7 cells were constantly grown as spheroidal aggregates and later formed a compact area of E-cadherin-positive tumor cells inside tumor compartment. Conclusion: Our study provides valuable knowledge on the properties of hBM-MSCs as vasculogenesis-supporting cells when co-cultured with MCF-7 cells on a 3D perfused biomimetic microfluidic device. This newly established model may serve as an experimental platform for testing anti-tumor/anti-angiogenic drugs.
MeSH term(s)	Animals ; Humans ; Female ; Coculture Techniques ; MCF-7 Cells ; Breast Neoplasms/pathology ; Endothelial Cells/pathology ; Microfluidics ; Biomimetics ; Bone Marrow/pathology ; Cell Differentiation ; Mesenchymal Stem Cells ; Cadherins ; Bone Marrow Cells ; Cells, Cultured
Chemical Substances	Cadherins
Language	English
Publishing date	2024-03-27
Publishing country	Greece
Document type	Journal Article
ZDB-ID	604549-2
ISSN	1791-7530 ; 0250-7005
ISSN (online)	1791-7530
ISSN	0250-7005
DOI	10.21873/anticanres.16940
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1642: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Tertiary Lymphoid Structures (TLSs) and Stromal Blood Vessels Have Significant and Heterogeneous Impact on Recurrence, Lymphovascular and Perineural Invasion amongst Breast Cancer Molecular Subtypes.

Barb, Alina Cristina / Pasca Fenesan, Mihaela / Pirtea, Marilena / Margan, Madalin Marius / Tomescu, Larisa / Melnic, Eugen / Cimpean, Anca Maria

Cells

2023 Volume 12, Issue 8

Abstract: Background: Tertiary lymphoid structures (TLSs) mediate local antitumor immunity, and interest in them significantly increased since cancer immunotherapy was implemented. We examined TLS- tumor stromal blood vessel interplay for each breast cancer (BC) ... ...

Abstract	Background: Tertiary lymphoid structures (TLSs) mediate local antitumor immunity, and interest in them significantly increased since cancer immunotherapy was implemented. We examined TLS- tumor stromal blood vessel interplay for each breast cancer (BC) molecular subtype related to recurrence, lymphovascular invasion (LVI), and perineural invasion (PnI). Methods: TLSs were quantified on hematoxylin and eosin stain specimens followed by CD34/smooth muscle actin (SMA) double immunostaining for stromal blood vessel maturation assessment. Statistical analysis linked microscopy to recurrence, LVI, and PnI. Results: TLS negative (TLS-) subgroups in each BC molecular subtype (except to Luminal A) have higher LVI, PnI, and recurrence. A significant rise in LVI and PnI were observed for the HER2+/TLS- subgroup ( Conclusion: BC invasion and recurrence are strongly influenced by TLS presence and stromal blood vessels, especially for HER2 and TNBC BC molecular subtypes.
MeSH term(s)	Humans ; Triple Negative Breast Neoplasms ; Tertiary Lymphoid Structures/pathology ; Neoplasm Invasiveness ; Breast/pathology
Language	English
Publishing date	2023-04-17
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells12081176
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Reassessing Breast Cancer-Associated Fibroblasts (CAFs) Interactions with Other Stromal Components and Clinico-Pathologic Parameters by Using Immunohistochemistry and Digital Image Analysis (DIA).

Barb, Alina Cristina / Fenesan, Mihaela Pasca / Pirtea, Marilena / Margan, Mădălin-Marius / Tomescu, Larisa / Ceban, Emil / Cimpean, Anca Maria / Melnic, Eugen

Cancers

2023 Volume 15, Issue 15

Abstract: Background: Breast cancer (BC) stroma has CD34- and αSMA-positive cancer-associated fibroblasts (CAFs) differently distributed. During malignant transformation, CD34-positive fibroblasts decrease while αSMA-positive CAFs increase. The prevalence of αSMA- ...

Abstract	Background: Breast cancer (BC) stroma has CD34- and αSMA-positive cancer-associated fibroblasts (CAFs) differently distributed. During malignant transformation, CD34-positive fibroblasts decrease while αSMA-positive CAFs increase. The prevalence of αSMA-positive CAFs in BC stroma makes microscopic examination difficult without digital image analysis processing (DIA). DIA was used to compare CD34- and αSMA-positive CAFs among breast cancer molecular subgroups. DIA-derived data were linked to age, survival, tumor stroma vessels, tertiary lymphoid structures (TLS), invasion, and recurrence. Methods: Double immunostaining for CD34 and αSMA showed different CAF distribution patterns in normal and BC tissues. Single CD34 immunohistochemistry on supplemental slides quantified tumor stroma CD34_CAFs. Digital image analysis (DIA) data on CAF density, intensity, stromal score, and H-score were correlated with clinico-pathologic factors. Results: CD34/αSMA CAF proportion was significantly related to age in Luminal A (LA), Luminal B (LB), and HER2 subtypes. CD34_CAF influence on survival, invasion, and recurrence of LA, LB-HER2, and TNBC subtypes was found to be significant. The CD34/αSMA-expressing CAFs exhibited a heterogeneous impact on stromal vasculature and TLS. Conclusion: BC stromal CD34_CAFs/αSMA_CAFs have an impact on survival, invasion, and recurrence differently between BC molecular subtypes. The tumor stroma DIA assessment may have predictive potential to prognosis and long-term follow-up of patients with breast cancer.
Language	English
Publishing date	2023-07-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15153823
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Synthesis, Physicochemical Characteristics, and Biocompatibility of Multi-Component Collagen-Based Hydrogels Developed by E-Beam Irradiation.

Demeter, Maria / Negrescu, Andreea Mariana / Calina, Ion / Scarisoreanu, Anca / Albu Kaya, Mădălina / Micutz, Marin / Dumitru, Marius / Cimpean, Anisoara

Journal of functional biomaterials

2023 Volume 14, Issue 9

Abstract: Herein, three different recipes of multi-component hydrogels were synthesized by e-beam irradiation. These hydrogels were obtained from aqueous polymer mixtures in which different proportions of bovine collagen gel, sodium carboxymethylcellulose (CMC), ... ...

Abstract	Herein, three different recipes of multi-component hydrogels were synthesized by e-beam irradiation. These hydrogels were obtained from aqueous polymer mixtures in which different proportions of bovine collagen gel, sodium carboxymethylcellulose (CMC), poly(vinylpyrrolidone), chitosan, and poly(ethylene oxide) were used. The cross-linking reaction was carried out exclusively by e-beam cross-linking at 25 kGy, a dose of irradiation sufficient both to complete the cross-linking reaction and effective for hydrogel sterilization. The hydrogels developed in this study were tested in terms of physical and chemical stability, mechanical, structural, morphological, and biological properties. They are transparent, maintain their structure, are non-adhesive when handling, and most importantly, especially from the application point of view, have an elastic structure. Likewise, these hydrogels possessed different swelling degrees and expressed rheological behavior characteristic of soft solids with permanent macromolecular network. Morphologically, collagen- and CMC based-hydrogels showed porous structures with homogeneously distributed pores assuring a good loading capacity with drugs. These hydrogels were investigated by indirect and direct contact studies with Vero cell line (CCL-81™, ATCC), demonstrating that they are well tolerated by normal cells and, therefore, showed promising potential for further use in the development of drug delivery systems based on hydrogels.
Language	English
Publishing date	2023-09-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2648525-4
ISSN	2079-4983
ISSN	2079-4983
DOI	10.3390/jfb14090454
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito