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Article ; Online: Doege-Potter syndrome.

Torres Gómez, Antonio / García Alabarce, Alba María / García Castro, José Miguel / Navas Bueno, Belén / Rueda Villafranca, Beatriz / Fernández, José Luis

Endocrinologia, diabetes y nutricion

2023

Language	English
Publishing date	2023-10-17
Publishing country	Spain
Document type	Case Reports
ISSN	2530-0180
ISSN (online)	2530-0180
DOI	10.1016/j.endien.2023.06.005
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Article ; Online: Implementing routine outcome monitoring in the treatment of a patient with obsessive-compulsive disorder.

Gómez, Beatriz / Fernández-Álvarez, Javier / García, Fernando

Journal of clinical psychology

2022 Volume 78, Issue 10, Page(s) 2002–2015

Abstract: This work presents a case study in which Cognitive-Integrative psychotherapy was used to treat a patient with obsessive-compulsive disorder with the implementation of routine outcome monitoring (ROM) at Aiglé Foundation in Argentina. The patient, a 34- ... ...

Abstract	This work presents a case study in which Cognitive-Integrative psychotherapy was used to treat a patient with obsessive-compulsive disorder with the implementation of routine outcome monitoring (ROM) at Aiglé Foundation in Argentina. The patient, a 34-year-old female with a university degree reports having obsessive ideas about dirt or pollution, causing harm to others, and religious and superstitious topics on a daily basis. The therapeutic process carried out during the first 17 psychotherapy sessions is described: construction of the therapeutic alliance, psychoeducation, and main interventions. The purpose of the article is to describe a case in which the measures' results did not show clinically relevant improvement even though the clinical indicators assessed by the therapist and the patient's subjective evaluation were positive. The article, shows the application of ROM during the supervision process, proposes interpretations of these results, and discusses decision-making processes in the light of this discrepancy.
MeSH term(s)	Adult ; Argentina ; Cognitive Behavioral Therapy/methods ; Female ; Humans ; Obsessive-Compulsive Disorder/therapy ; Psychotherapy ; Treatment Outcome
Language	English
Publishing date	2022-07-23
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	219160-x
ISSN	1097-4679 ; 0021-9762
ISSN (online)	1097-4679
ISSN	0021-9762
DOI	10.1002/jclp.23418
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Article ; Online: Updated antimicrobial dosing recommendations for obese patients.

Castro-Balado, Ana / Varela-Rey, Iria / Mejuto, Beatriz / Mondelo-García, Cristina / Zarra-Ferro, Irene / Rodríguez-Jato, Teresa / Fernández-Ferreiro, Anxo

Antimicrobial agents and chemotherapy

2024 , Page(s) e0171923

Abstract: The prevalence of obesity has increased considerably in the last few decades. Pathophysiological changes in obese patients lead to pharmacokinetic (PK) and pharmacodynamic (PD) alterations that can condition the correct exposure to antimicrobials if ... ...

Abstract	The prevalence of obesity has increased considerably in the last few decades. Pathophysiological changes in obese patients lead to pharmacokinetic (PK) and pharmacodynamic (PD) alterations that can condition the correct exposure to antimicrobials if standard dosages are used. Inadequate dosing in obese patients can lead to toxicity or therapeutic failure. In recent years, additional antimicrobial PK/PD data, extended infusion strategies, and studies in critically ill patients have made it possible to obtain data to provide a better dosage in obese patients. Despite this, it is usually difficult to find information on drug dosing in this population, which is sometimes contradictory. This is a comprehensive review of the dosing of different types of antimicrobials (antibiotics, antifungals, antivirals, and antituberculosis drugs) in obese patients, where the literature on PK and possible dosing strategies in obese adults was critically assessed.
Language	English
Publishing date	2024-03-25
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/aac.01719-23
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Article ; Online: Description of a new quantitative method to assess mitochondrial distribution pattern in mature equine oocytes.

Luis-Calero, Marcos / Fernández-Hernández, Pablo / Ortiz-Rodríguez, José Manuel / Muñoz-García, Carmen Cristina / Jardin, Isaac / Macías-García, Beatriz / González-Fernández, Lauro

Veterinary research communications

2024

Abstract: The Mitochondrial distribution pattern or MDP in mammalian oocytes serves as an indicator of their cytoplasmic maturity, with a heterogeneous pattern associated with mature cytoplasm. Currently, MDP assessment involves fluorescent labelling of ... ...

Abstract	The Mitochondrial distribution pattern or MDP in mammalian oocytes serves as an indicator of their cytoplasmic maturity, with a heterogeneous pattern associated with mature cytoplasm. Currently, MDP assessment involves fluorescent labelling of mitochondria followed by visual evaluation, as no quantitative method exists. Our objective was to develop a quantitative approach to assess MDP in mature equine oocytes. Equine oocytes, obtained by ovum pick up (OPU) were matured in vitro, and only metaphase II oocytes were used in the study (n = 56). Following denudation, oocytes were fixed, stained with MitoTracker™ Red CMXRos (50 nM in TCM-199 with Hank´s salts and 10% FBS) for 15 min at 38 °C, and then incubated with 2.5 µg/ml Hoechst 33342 for 10 min at 38 °C. Confocal microscope images were acquired, and the oocyte's MDP was visually classified as either homogeneous (HoD; n = 17) or heterogeneous (HeD; n = 39). For quantitative analysis, Fiji-ImageJ software was employed. Background subtraction was performed, and a 1-pixel line along the diameter was drawn to calculate the intensity profile. Fluorescence intensities were normalized, and ratios of peripheral to central fluorescence intensity were determined. Student´s t-test was used for comparations; MDP ratio was (mean ± standard error of the mean): 0.8 ± 0.02 for HoD and 0.3 ± 0.02 for HeD (p < 0.001). These results demonstrate concordance between quantitative and qualitative MDP assessment in mature equine oocytes. Our study describes a new approach to quantify mitochondrial distribution pattern and cytoplasmic maturation in mature equine oocytes.
Language	English
Publishing date	2024-02-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	406735-6
ISSN	1573-7446 ; 0165-7380
ISSN (online)	1573-7446
ISSN	0165-7380
DOI	10.1007/s11259-024-10325-z
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Article ; Online: Design of a Stem Cell-Based Therapy for Ependymal Repair in Hydrocephalus Associated With Germinal Matrix Hemorrhages.

Rodriguez-Perez, Luis M / Ojeda-Pérez, Betsaida / López-de-San-Sebastián, Javier / García-Bonilla, María / González-García, Marcos / Fernández-Muñoz, Beatriz / Sánchez-Pernaute, Rosario / García-Martín, María L / Domínguez-Pinos, Dolores / Cárdenas-García, Casimiro / Jiménez, Antonio J / Paez-Gonzalez, Patricia

Stroke

2024 Volume 55, Issue 4, Page(s) 1062–1074

Abstract: Background: In preterm birth germinal matrix hemorrhages (GMHs) and the consequent posthemorrhagic hydrocephalus (PHH), the neuroepithelium/ependyma development is disrupted. This work is aimed to explore the possibilities of ependymal repair in GMH/PHH ...

Abstract	Background: In preterm birth germinal matrix hemorrhages (GMHs) and the consequent posthemorrhagic hydrocephalus (PHH), the neuroepithelium/ependyma development is disrupted. This work is aimed to explore the possibilities of ependymal repair in GMH/PHH using a combination of neural stem cells, ependymal progenitors (EpPs), and mesenchymal stem cells. Methods: GMH/PHH was induced in 4-day-old mice using collagenase, blood, or blood serum injections. PHH severity was characterized 2 weeks later using magnetic resonance, immunofluorescence, and protein expression quantification with mass spectrometry. Ependymal restoration and wall regeneration after stem cell treatments were tested in vivo and in an ex vivo experimental approach using ventricular walls from mice developing moderate and severe GMH/PHH. The effect of the GMH environment on EpP differentiation was tested in vitro. Two-tailed Student Results: PHH severity was correlated with the extension of GMH and ependymal disruption (means, 88.22% severe versus 19.4% moderate). GMH/PHH hindered the survival rates of the transplanted neural stem cells/EpPs. New multiciliated ependymal cells could be generated from transplanted neural stem cells and more efficiently from EpPs (15% mean increase). Blood and TNFα (tumor necrosis factor alpha) negatively affected ciliogenesis in cells committed to ependyma differentiation (expressing Foxj1 [forkhead box J1] transcription factor). Pretreatment with mesenchymal stem cells improved the survival rates of EpPs and ependymal differentiation while reducing the edematous (means, 18% to 0.5% decrease in severe edema) and inflammatory conditions in the explants. The effectiveness of this therapeutical strategy was corroborated in vivo (means, 29% to 0% in severe edema). Conclusions: In GMH/PHH, the ependyma can be restored and edema decreased from either neural stem cell or EpP transplantation in vitro and in vivo. Mesenchymal stem cell pretreatment improved the success of the ependymal restoration.
MeSH term(s)	Humans ; Female ; Animals ; Mice ; Ependyma/pathology ; Premature Birth ; Hydrocephalus/surgery ; Hydrocephalus/metabolism ; Cerebral Hemorrhage/therapy ; Cerebral Hemorrhage/metabolism ; Fetal Diseases ; Neural Stem Cells ; Edema
Language	English
Publishing date	2024-03-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	80381-9
ISSN	1524-4628 ; 0039-2499 ; 0749-7954
ISSN (online)	1524-4628
ISSN	0039-2499 ; 0749-7954
DOI	10.1161/STROKEAHA.123.044677
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Article ; Online: Chemical-Physical Model of Gaseous Mercury Emissions from the Demolition Waste of an Abandoned Mercury Metallurgical Plant

Rafael Rodríguez / Begoña Fernández / Beatriz Malagón / Efrén Garcia-Ordiales

Applied Sciences, Vol 13, Iss 3149, p

2023 Volume 3149

Abstract: Soils from decommissioned Hg mine sites usually exhibit high levels of total mercury concentration. This work examines the behavior of mercury in the atmosphere on samples of contaminated debris of a demolished metallurgical plant present in La Soterraña ...

Abstract	Soils from decommissioned Hg mine sites usually exhibit high levels of total mercury concentration. This work examines the behavior of mercury in the atmosphere on samples of contaminated debris of a demolished metallurgical plant present in La Soterraña mine, Asturias (Spain). Previously, a strong dependence of the Hg gas concentration C max (ng/m 3 ) with the temperature T (K) was determined empirically. Hg gas concentration varied between 6500 ng/m 3 at low temperatures, 278 K (5 °C), and up to almost 60,000 ng/m 3 when the temperature reaches 303 K (30 °C). Then, two different models were proposed to explain the behavior of the mercury emitted from this source. The first model is based on Arrhenius theory. The gas flux per unit area perpendicular to the flow F (g/sm 2 ) is an exponential function of the apparent activation energy E a (J/mol): F = c f exp(-E a /RT). The values of c f = 1.04·10 7 and E a = 48.56 kJ/mol allows the model to fit well with the field measurements. The second model is based on Fick’s laws, and the flux F (g/sm 2 ) can be estimated by F = (K′ M Hg p v )/RT where K′ = 8.49·10 −7 , M Hg = 200.56 g/mol and the partial vapor pressure of gaseous mercury p v (Pa) can be estimated from the saturation vapor pressure of gaseous mercury p v = 0.00196·p s and the August’s law log(p s ) = 10.184–3210.29/T. This method is also validated with results measured in situ. Both methods are accurate enough to explain and predict emission rate G (g/s), gas flux F (g/sm 2 ) and maximum Hg gas concentration over the debris C max (ng/m 3 ) as a function the temperature T (K).
Keywords	mercury ; mobility ; debris ; modelling ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	333
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Genistein early in life Modifies the arcuate nucleus of the hypothalamus morphology differentially in male and female rats

Fernandez-Garcia, Jose Manuel / Carrillo, Beatriz / Tezanos, Patricia / Pinos, Helena / Collado, Paloma

Molecular and Cellular Endocrinology. 2023 Apr. 18, p.111933-

2023 , Page(s) 111933–

Abstract: In the present work we analyzed the effects of postnatal exposure to two doses of genistein (10 μg/g or 50 μg/g) from postnatal (P) day 6 to P13, on the morphology of the arcuate nucleus (Arc). The analyses of Arc coronal brain sections at 90 days showed ...

Abstract	In the present work we analyzed the effects of postnatal exposure to two doses of genistein (10 μg/g or 50 μg/g) from postnatal (P) day 6 to P13, on the morphology of the arcuate nucleus (Arc). The analyses of Arc coronal brain sections at 90 days showed that the ArcMP had higher values in volume, Nissl-stained neurons and GPER-ir neurons in males than in females and the treatment with genistein abolished these sex differences in most of the parameters studied. Moreover, in males, but not in females, the GPER-ir neurons decreased in the ArcMP but increased in the ArcL with both doses of genistein. In the ArcLP, GPER-ir population increased with the lowest doses and decreased with the highest one in males. Our results confirm that the Arc subdivisions have differential vulnerability to the effects of genistein during development, depending on which neuromorphological parameters, dose and sex are analyzed.
Keywords	endocrinology ; females ; genistein ; hypothalamus ; males ; Arcuate nucleus ; GPER ; Nissl-stained neurons ; Sexual dimorphism
Language	English
Dates of publication	2023-0418
Publishing place	Elsevier B.V.
Document type	Article ; Online
Note	Pre-press version ; Use and reproduction
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2023.111933
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Pulmonary Malt Lymphoma With Amyloidosis: An Uncommon Pulmonary Entity.

García Pulido, Beatriz / Fernández Fernández, Alejandro

Archivos de bronconeumologia

2023 Volume 60, Issue 2, Page(s) 107–108

MeSH term(s)	Humans ; Lymphoma, B-Cell, Marginal Zone/complications ; Lymphoma, B-Cell, Marginal Zone/pathology ; Lung/pathology ; Amyloidosis/complications ; Amyloidosis/pathology ; Bronchial Neoplasms/pathology
Language	Spanish
Publishing date	2023-11-21
Publishing country	Spain
Document type	Case Reports
ZDB-ID	733126-5
ISSN	1579-2129 ; 0300-2896
ISSN (online)	1579-2129
ISSN	0300-2896
DOI	10.1016/j.arbres.2023.10.013
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Article ; Online: Genistein early in life modifies the arcuate nucleus of the hypothalamus morphology differentially in male and female rats.

Fernandez-Garcia, Jose Manuel / Carrillo, Beatriz / Tezanos, Patricia / Pinos, Helena / Collado, Paloma

Molecular and cellular endocrinology

2023 Volume 570, Page(s) 111933

Abstract	In the present work we analyzed the effects of postnatal exposure to two doses of genistein (10 μg/g or 50 μg/g) from postnatal (P) day 6 to P13, on the morphology of the arcuate nucleus (Arc). The analyses of Arc coronal brain sections at 90 days showed that the ArcMP had higher values in volume, Nissl-stained neurons and GPER-ir neurons in males than in females and the treatment with genistein abolished these sex differences in most of the parameters studied. Moreover, in males, but not in females, the GPER-ir neurons decreased in the ArcMP but increased in the ArcL with both doses of genistein. In the ArcLP, GPER-ir population increased with the lowest doses and decreased with the highest one in males. Our results confirm that the Arc subdivisions have differential vulnerability to the effects of genistein during development, depending on which neuromorphological parameters, dose and sex are analyzed.
MeSH term(s)	Rats ; Animals ; Female ; Male ; Arcuate Nucleus of Hypothalamus ; Genistein/pharmacology ; Hypothalamus ; Neurons ; Sex Characteristics
Chemical Substances	Genistein (DH2M523P0H)
Language	English
Publishing date	2023-04-18
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2023.111933
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Article ; Online: Implementation of an Ecological Momentary Assessment (EMA) in Naturalistic Psychotherapy Settings: Qualitative Insights from Patients, Therapists, and Supervisors Perspectives.

Meglio, Manuel / Manubens, Rocío Tamara / Fernández-Álvarez, Javier / Marasas, Sofia / García, Fernando / Gómez, Beatríz / Montedoro, Julio / Jáuregui, Antonio Nicolás / Castañeiras, Claudia / Santagnelo, Pablo / Juan, Santiago / Roussos, Andrés Jorge / Gómez Penedo, Juan Martín / Muiños, Roberto

Administration and policy in mental health

2024

Abstract: Ecological momentary assessment (EMA) allows measuring intra-individual processes moment by moment, identifying and modeling, in a naturalistic way, individual levels and changes in different psychological processes. However, active EMA requires a high ... ...

Abstract	Ecological momentary assessment (EMA) allows measuring intra-individual processes moment by moment, identifying and modeling, in a naturalistic way, individual levels and changes in different psychological processes. However, active EMA requires a high degree of adherence, as it implies a significant burden for patients. Moreover, there is still no consensus on standardized procedures for implementation. There have been few results in detecting desirable characteristics for the design and implementation of an EMA device. Studies that address these issues from the perspectives of participants in psychotherapeutic processes are needed. To analyze the perspectives of patients, therapists and supervisors on the implementation of an EMA device in a psychotherapeutic treatment for depression. The sample will include eight patients, eleven therapists and five supervisors, taken from a research project that implemented an EMA system for monitoring the dynamics of affectivity at the beginning of psychotherapies for depression. Semi-structured interviews specific to each group are being conducted and analyzed from a qualitative approach based on consensual qualitative research (CQR). Participants reported having a positive evaluation of the study's informational resources and implementation. Difficulties were expressed in responding in the morning hours and the importance of having a customized EMA that is tailored to the needs of the patients was expressed. Furthermore, patients and therapists agreed that the impact of the use of the monitoring system on treatment was neutral or positive. In contrast, patients considered the EMA to be positive for their daily life.
Language	English
Publishing date	2024-03-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	1025319-1
ISSN	1573-3289 ; 0894-587X
ISSN (online)	1573-3289
ISSN	0894-587X
DOI	10.1007/s10488-024-01362-6
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