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Article ; Online: Factors influencing dietary inequalities among adolescents in Madrid and Bilbao: a qualitative research project.

Rivera-Navarro, Jesús / de Loyola González-Salgado, Ignacio / Ramos-Truchero, Guadalupe / Gravina, Leyre / Díez, Julia / Caballero, Silvia / Franco, Manuel

Gaceta sanitaria

2023 Volume 37, Page(s) 102325

Abstract: Objective: To describe the design and methodology of a qualitative study to explore the main factors influencing dietary inequalities in adolescents in Madrid and Bilbao, Spain.: Method: The study area included six neighborhoods (three in each city) ... ...

Abstract	Objective: To describe the design and methodology of a qualitative study to explore the main factors influencing dietary inequalities in adolescents in Madrid and Bilbao, Spain. Method: The study area included six neighborhoods (three in each city) of different socioeconomic status (SES): low, medium, and high. We sampled 12 secondary schools (six in each city: two per socioeconomic level). Our methodology comprised: 1) developing an ad hoc index to classify all neighborhoods according to their SES; 2) selecting the study area and sample; 3) conducting semi-structured interviews (n=36) and focus groups (n=24). Grounded theory and phenomenological analysis will be employed in data analysis. Initially, we found factors influencing in adolescents' diet such as gender, family environment, and SES. Conclusions: Systematizing the selection of neighborhoods and secondary schools, along with using appropriate methods, could serve as a foundation for future studies on health inequalities among adolescents.
MeSH term(s)	Humans ; Adolescent ; Qualitative Research ; Diet ; Research Design ; Focus Groups ; Data Analysis
Language	English
Publishing date	2023-08-18
Publishing country	Spain
Document type	Journal Article
ZDB-ID	1038713-4
ISSN	1578-1283 ; 0213-9111
ISSN (online)	1578-1283
ISSN	0213-9111
DOI	10.1016/j.gaceta.2023.102325
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Article ; Online: Design and development of a hepatic lyo-dECM powder as a biomimetic component for 3D-printable hybrid hydrogels.

Di Gravina, Giulia M / Bari, Elia / Croce, Stefania / Scocozza, Franca / Pisani, Silvia / Conti, Bice / Avanzini, Maria A / Auricchio, Ferdinando / Cobianchi, Lorenzo / Torre, Maria Luisa / Conti, Michele

Biomedical materials (Bristol, England)

2023 Volume 19, Issue 1

Abstract: Bioprinting offers new opportunities to obtain reliable ... ...

Abstract	Bioprinting offers new opportunities to obtain reliable 3D
MeSH term(s)	Swine ; Animals ; Humans ; Extracellular Matrix ; Powders ; Hydrogels ; Biomimetics ; Printing, Three-Dimensional ; Liver ; Bioprinting/methods ; Tissue Scaffolds ; Tissue Engineering
Chemical Substances	Powders ; Hydrogels
Language	English
Publishing date	2023-12-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2265222-X
ISSN	1748-605X ; 1748-6041
ISSN (online)	1748-605X
ISSN	1748-6041
DOI	10.1088/1748-605X/ad0ee2
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Article ; Online: Factors influencing dietary inequalities among adolescents in Madrid and Bilbao

Jesús Rivera-Navarro / Ignacio de Loyola González-Salgado / Guadalupe Ramos-Truchero / Leyre Gravina / Julia Díez / Silvia Caballero / Manuel Franco

Gaceta Sanitaria, Vol 37, Iss , Pp 102325- (2023)

a qualitative research project

2023

Abstract: Objective: To describe the design and methodology of a qualitative study to explore the main factors influencing dietary inequalities in adolescents in Madrid and Bilbao, Spain. Method: The study area included six neighborhoods (three in each city) of ... ...

Abstract	Objective: To describe the design and methodology of a qualitative study to explore the main factors influencing dietary inequalities in adolescents in Madrid and Bilbao, Spain. Method: The study area included six neighborhoods (three in each city) of different socioeconomic status (SES): low, medium, and high. We sampled 12 secondary schools (six in each city: two per socioeconomic level). Our methodology comprised: 1) developing an ad hoc index to classify all neighborhoods according to their SES; 2) selecting the study area and sample; 3) conducting semi-structured interviews (n = 36) and focus groups (n = 24). Grounded theory and phenomenological analysis will be employed in data analysis. Initially, we found factors influencing in adolescents’ diet such as gender, family environment, and SES. Conclusions: Systematizing the selection of neighborhoods and secondary schools, along with using appropriate methods, could serve as a foundation for future studies on health inequalities among adolescents. Resumen: Objetivo: Describir el diseño y la metodología de un estudio cualitativo que explora los factores principales que influyen en la desigualdad alimentaria en los adolescentes en Madrid y Bilbao, España. Método: Se seleccionaron seis barrios (tres en cada ciudad) de diferente estatus socioeconómico (ESE): bajo, medio y alto. Obtuvimos una muestra de 12 institutos (seis en cada ciudad y dos por nivel socioeconómico). La metodología consistió en: 1) desarrollo de un índice ad hoc para clasificar los barrios según su ESE; 2) selección de las áreas de estudio y de institutos; 3) realización de entrevistas semiestructuradas (n = 36) y grupos de discusión (n = 24). En el análisis se utilizarán la teoría fundamentada y el análisis fenomenológico. Inicialmente, encontramos factores que influyen en la alimentación de los adolescentes, como el género, el entorno familiar y el ESE. Conclusiones: La sistematización en la selección de barrios e institutos, y la utilización de metodología adecuada, pueden servir como base ...
Keywords	Desigualdad social ; Adolescente ; Entorno social ; Investigación cualitativa ; Colegios ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Role of Diacylglycerol Kinases in Acute Myeloid Leukemia.

Gravina, Teresa / Boggio, Chiara Maria Teresa / Gorla, Elisa / Racca, Luisa / Polidoro, Silvia / Centonze, Sara / Ferrante, Daniela / Lunghi, Monia / Graziani, Andrea / Corà, Davide / Baldanzi, Gianluca

Biomedicines

2023 Volume 11, Issue 7

Abstract: Diacylglycerol kinases (DGKs) play dual roles in cell transformation and immunosurveillance. According to cancer expression databases, acute myeloid leukemia (AML) exhibits significant overexpression of multiple DGK isoforms, ... ...

Abstract	Diacylglycerol kinases (DGKs) play dual roles in cell transformation and immunosurveillance. According to cancer expression databases, acute myeloid leukemia (AML) exhibits significant overexpression of multiple DGK isoforms, including
Language	English
Publishing date	2023-07-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines11071877
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Article: Frailty Network in an Acute Care Setting: The New Perspective for Frail Older People.

Salini, Sara / Giovannini, Silvia / Covino, Marcello / Barillaro, Christian / Acampora, Nicola / Gravina, Ester Manes / Loreti, Claudia / Damiano, Francesco Paolo / Franceschi, Francesco / Russo, Andrea

Diagnostics (Basel, Switzerland)

2022 Volume 12, Issue 5

Abstract: The incidence of elderly patients who come to the emergency room is progressively increasing. The specialization of the physician units might not be adequate for the evaluation of this complexity. The present study aimed to present a standard procedure, ... ...

Abstract	The incidence of elderly patients who come to the emergency room is progressively increasing. The specialization of the physician units might not be adequate for the evaluation of this complexity. The present study aimed to present a standard procedure, called 'The Geriatric Frailty Network', operating at the Policlinico Gemelli IRCCS Foundation, which is configured specifically for the level II assessment of frail elderly patients. This was a retrospective study in 1191 patients aged over 65, who were evaluated by the Geriatric Frailty Unit directly after emergency department admission for one year. All patients underwent multidimensional geriatric evaluation. Data were collected on demographics, co-morbidity, disease severity, and Clinical Frailty Scale. Among all patients, 723 were discharged directly from the emergency room with early identification of continuity of care path. Globally, 468 patients were hospitalized with an early assessment of frailty that facilitated the discharge process. The geriatric frailty network model aims to assist the emergency room and ward doctor in the prevention of the most common geriatric syndromes and reduce the number of incongruous hospitalizations.
Language	English
Publishing date	2022-05-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics12051228
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Article ; Online: The dark side of circulating nucleic acids.

Gravina, Silvia / Sedivy, John M / Vijg, Jan

Aging cell

2016 Volume 15, Issue 3, Page(s) 398–399

Abstract: Free circulating or cell-free DNA (cfDNA), possibly from dying cells that release their contents into the blood as they break down, have become of major interest as a source for noninvasive diagnostics. Recent work demonstrated the uptake of human cfDNA ... ...

Abstract	Free circulating or cell-free DNA (cfDNA), possibly from dying cells that release their contents into the blood as they break down, have become of major interest as a source for noninvasive diagnostics. Recent work demonstrated the uptake of human cfDNA in mouse cells in vitro and in vivo, accompanied by the activation of a cellular DNA damage response (DDR) and the appearance of apoptotic proteins in the host cells. By acting as a source of mobile genetic elements, cfDNA could be a continuous source of DNA mutagenesis of healthy cells in the body throughout life, promoting progressive cellular aging in vivo. As such, cfDNA may causally contribute to multiple aging-related diseases, such as cancer, diabetes, and Alzheimer's disease.
MeSH term(s)	Animals ; DNA Damage ; Humans ; Mutagenesis/genetics ; Nucleic Acids/blood
Chemical Substances	Nucleic Acids
Language	English
Publishing date	2016-06
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2113083-8
ISSN	1474-9726 ; 1474-9718
ISSN (online)	1474-9726
ISSN	1474-9718
DOI	10.1111/acel.12454
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Article ; Online: Single-cell genome-wide bisulfite sequencing uncovers extensive heterogeneity in the mouse liver methylome.

Gravina, Silvia / Dong, Xiao / Yu, Bo / Vijg, Jan

Genome biology

2016 Volume 17, Issue 1, Page(s) 150

Abstract: Background: Transmission fidelity of CpG DNA methylation patterns is not foolproof, with error rates from less than 1 to well over 10 % per CpG site, dependent on preservation of the methylated or unmethylated state and the type of sequence. This ... ...

Abstract	Background: Transmission fidelity of CpG DNA methylation patterns is not foolproof, with error rates from less than 1 to well over 10 % per CpG site, dependent on preservation of the methylated or unmethylated state and the type of sequence. This suggests a fairly high chance of errors. However, the consequences of such errors in terms of cell-to-cell variation have never been demonstrated by experimentally measuring intra-tissue heterogeneity in an adult organism. Results: We employ single-cell DNA methylomics to analyze heterogeneity of genome-wide 5-methylcytosine (5mC) patterns within mouse liver. Our results indicate a surprisingly high level of heterogeneity, corresponding to an average epivariation frequency of approximately 3.3 %, with regions containing H3K4me1 being the most variable and promoters and CpG islands the most stable. Our data also indicate that the level of 5mC heterogeneity is dependent on genomic features. We find that non-functional sites such as repeat elements and introns are mostly unstable and potentially functional sites such as gene promoters are mostly stable. Conclusions: By employing a protocol for whole-genome bisulfite sequencing of single cells, we show that the liver epigenome is highly unstable with an epivariation frequency in DNA methylation patterns of at least two orders of magnitude higher than somatic mutation frequencies.
MeSH term(s)	5-Methylcytosine/metabolism ; Animals ; CpG Islands/genetics ; DNA Methylation/genetics ; Genetic Heterogeneity ; Genome ; High-Throughput Nucleotide Sequencing/methods ; Liver/metabolism ; Mice ; Mutation ; Promoter Regions, Genetic ; Single-Cell Analysis/methods
Chemical Substances	5-Methylcytosine (6R795CQT4H)
Language	English
Publishing date	2016-07-05
Publishing country	England
Document type	Journal Article
ZDB-ID	2040529-7
ISSN	1474-760X ; 1465-6914 ; 1465-6906
ISSN (online)	1474-760X ; 1465-6914
ISSN	1465-6906
DOI	10.1186/s13059-016-1011-3
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Article: Single-cell genome-wide bisulfite sequencing uncovers extensive heterogeneity in the mouse liver methylome

Gravina, Silvia / Dong, Xiao / Yu, Bo / Vijg, Jan

Genome biology. 2016 Dec., v. 17, no. 1

2016

Abstract: BACKGROUND: Transmission fidelity of CpG DNA methylation patterns is not foolproof, with error rates from less than 1 to well over 10 % per CpG site, dependent on preservation of the methylated or unmethylated state and the type of sequence. This ... ...

Abstract	BACKGROUND: Transmission fidelity of CpG DNA methylation patterns is not foolproof, with error rates from less than 1 to well over 10 % per CpG site, dependent on preservation of the methylated or unmethylated state and the type of sequence. This suggests a fairly high chance of errors. However, the consequences of such errors in terms of cell-to-cell variation have never been demonstrated by experimentally measuring intra-tissue heterogeneity in an adult organism. RESULTS: We employ single-cell DNA methylomics to analyze heterogeneity of genome-wide 5-methylcytosine (5mC) patterns within mouse liver. Our results indicate a surprisingly high level of heterogeneity, corresponding to an average epivariation frequency of approximately 3.3 %, with regions containing H3K4me1 being the most variable and promoters and CpG islands the most stable. Our data also indicate that the level of 5mC heterogeneity is dependent on genomic features. We find that non-functional sites such as repeat elements and introns are mostly unstable and potentially functional sites such as gene promoters are mostly stable. CONCLUSIONS: By employing a protocol for whole-genome bisulfite sequencing of single cells, we show that the liver epigenome is highly unstable with an epivariation frequency in DNA methylation patterns of at least two orders of magnitude higher than somatic mutation frequencies.
Keywords	liver ; genomic islands ; DNA methylation ; DNA ; mice ; somatic mutation ; introns ; adults
Language	English
Dates of publication	2016-12
Size	p. 150.
Publishing place	BioMed Central
Document type	Article
Note	2019-12-06
ZDB-ID	2040529-7
ISSN	1474-760X ; 1465-6906
ISSN (online)	1474-760X
ISSN	1465-6906
DOI	10.1186/s13059-016-1011-3
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns.

Gravina, Silvia / Ganapathi, Shireen / Vijg, Jan

Nucleic acids research

2015 Volume 43, Issue 14, Page(s) e93

Abstract: Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and ... ...

Abstract	Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we present a novel procedure for directly detecting epimutations in DNA methylation patterns using single-cell, locus-specific bisulfite sequencing (SLBS). We show that within gene promoter regions of mouse hepatocytes the epimutation rate is two orders of magnitude higher than the mutation rate.
MeSH term(s)	Animals ; Cells, Cultured ; DNA Methylation ; Epigenesis, Genetic ; Genetic Loci ; Mice ; Mice, Inbred C57BL ; Mutation Rate ; Promoter Regions, Genetic ; Sequence Analysis, DNA/methods ; Single-Cell Analysis ; Sulfites
Chemical Substances	Sulfites ; hydrogen sulfite (OJ9787WBLU)
Language	English
Publishing date	2015-08-18
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	186809-3
ISSN	1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
ISSN (online)	1362-4962 ; 1362-4954
ISSN	0301-5610 ; 0305-1048
DOI	10.1093/nar/gkv366
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Article ; Online: MS-275 (Entinostat) Promotes Radio-Sensitivity in PAX3-FOXO1 Rhabdomyosarcoma Cells.

International journal of molecular sciences

2021 Volume 22, Issue 19

Abstract: Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood. About 25% of RMS expresses fusion oncoproteins such as PAX3/PAX7-FOXO1 (fusion-positive, FP) while fusion-negative (FN)-RMS harbors RAS mutations. Radiotherapy (RT) plays a ... ...

Abstract	Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood. About 25% of RMS expresses fusion oncoproteins such as PAX3/PAX7-FOXO1 (fusion-positive, FP) while fusion-negative (FN)-RMS harbors RAS mutations. Radiotherapy (RT) plays a crucial role in local control but metastatic RMS is often radio-resistant. HDAC inhibitors (HDACi) radio-sensitize different cancer cells types. Thus, we evaluated MS-275 (Entinostat), a Class I and IV HDACi, in combination with RT on RMS cells in vitro and in vivo. MS-275 reversibly hampered cell survival in vitro in FN-RMS RD (RASmut) and irreversibly in FP-RMS RH30 cell lines down-regulating cyclin A, B, and D1, up-regulating p21 and p27 and reducing ERKs activity, and c-Myc expression in RD and PI3K/Akt/mTOR activity and N-Myc expression in RH30 cells. Further, MS-275 and RT combination reduced colony formation ability of RH30 cells. In both cell lines, co-treatment increased DNA damage repair inhibition and reactive oxygen species formation, down-regulated
MeSH term(s)	Animals ; Apoptosis/drug effects ; Benzamides/pharmacology ; Cell Cycle Checkpoints/drug effects ; Cell Cycle Checkpoints/radiation effects ; Cell Line, Tumor ; Cell Proliferation/drug effects ; DNA Damage/drug effects ; DNA Repair/drug effects ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Mice ; Oncogene Proteins, Fusion/genetics ; Paired Box Transcription Factors/genetics ; Pyridines/pharmacology ; Radiation Tolerance/drug effects ; Radiation Tolerance/genetics ; Radiation-Sensitizing Agents/pharmacology ; Rhabdomyosarcoma/genetics ; Rhabdomyosarcoma/metabolism ; Rhabdomyosarcoma/radiotherapy
Chemical Substances	Benzamides ; Oncogene Proteins, Fusion ; PAX3-FOXO1A fusion protein, human ; Paired Box Transcription Factors ; Pyridines ; Radiation-Sensitizing Agents ; entinostat (1ZNY4FKK9H)
Language	English
Publishing date	2021-10-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms221910671
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