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  1. Article ; Online: Compartmentalization at the interface of primary and alkaloid metabolism.

    Watkins, Jacinta L / Facchini, Peter J

    Current opinion in plant biology

    2022  Volume 66, Page(s) 102186

    Abstract: Plants produce many compounds used by humans as medicines, including alkaloids of the benzylisoquinoline (BIA), monoterpene indole (MIA) and tropane classes. The biosynthetic pathways of these pharmaceutical alkaloids are complex and spatially segregated ...

    Abstract Plants produce many compounds used by humans as medicines, including alkaloids of the benzylisoquinoline (BIA), monoterpene indole (MIA) and tropane classes. The biosynthetic pathways of these pharmaceutical alkaloids are complex and spatially segregated across several tissues, cell-types and organelles. This review discusses the origin of primary metabolic inputs required by these specialized biosynthetic pathways and considers aspects relevant to their spatial organization. These factors are important for alkaloid production both in the native plants and for synthetic biology pathway reconstruction in microorganisms.
    MeSH term(s) Alkaloids/metabolism ; Biosynthetic Pathways ; Plants/metabolism ; Synthetic Biology
    Chemical Substances Alkaloids
    Language English
    Publishing date 2022-02-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1418472-2
    ISSN 1879-0356 ; 1369-5266
    ISSN (online) 1879-0356
    ISSN 1369-5266
    DOI 10.1016/j.pbi.2022.102186
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2005/717: Show issues

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  2. Article ; Online: Characterization of Four Alkyl-branched Fatty Acids as Methyl, Ethyl, Propyl, and Butyl Esters Using Gas Chromatography-Quadrupole Time of Flight Mass Spectrometry.

    Watkins, Peter J

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    2019  Volume 36, Issue 4, Page(s) 425–429

    Abstract: Branched fatty chain fatty acids (BCFAs) are associated with the "mutton flavor" found with the aroma resulting from cooked older sheep meat with three BCFAs, 4-methyloctanoic (MOA), 4-ethyloctanoic (EOA) and 4-methylnonanoic (MNA) acids as the main ... ...

    Abstract Branched fatty chain fatty acids (BCFAs) are associated with the "mutton flavor" found with the aroma resulting from cooked older sheep meat with three BCFAs, 4-methyloctanoic (MOA), 4-ethyloctanoic (EOA) and 4-methylnonanoic (MNA) acids as the main compounds responsible for "mutton flavor". Usually, BCFA analysis is done by gas chromatography (GC) with the use of quadrupole mass spectrometry (qMS) becoming predominant. 2-Butyloctanoic acid (2BO) has been used in this facility using as an internal standard to determine BCFA content in sheep fat. In this present work, GC-qMS, along with GC-quadrupole-time of flight MS (GC-QTOF-MS), have been deployed to characterize alkyl esters (as methyl, ethyl, propyl, and butyl) for MOA, EOA, MNA, and 2BO. This work presents, for the first time, the mass spectral characterization of 2BO for these alkyl esters using GC-qMS and GC-QTOF-MS.
    Language English
    Publishing date 2019-11-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1483376-1
    ISSN 1348-2246 ; 1348-2246
    ISSN (online) 1348-2246
    ISSN 1348-2246
    DOI 10.2116/analsci.19P349
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book: Diabetes and its management

    Watkins, Peter J.

    2003  

    Author's details Peter J. Watkins
    Keywords Diabetes Mellitus ; Diabetes Mellitus / therapy ; Diabetes ; Diabetes/Treatment
    Subject code 616.462
    Language English
    Size XI, 224 S.
    Edition 6. ed.
    Publisher Blackwell
    Publishing place Oxford
    Publishing country Great Britain
    Document type Book
    HBZ-ID HT013826805
    ISBN 1-4051-0725-1 ; 978-1-4051-0725-9
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2003 A 3981 order for loan Magazin

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  4. Article ; Online: Elucidation of the mescaline biosynthetic pathway in peyote (Lophophora williamsii).

    Watkins, Jacinta L / Li, Qiushi / Yeaman, Sam / Facchini, Peter J

    The Plant journal : for cell and molecular biology

    2023  Volume 116, Issue 3, Page(s) 635–649

    Abstract: Peyote (Lophophora williamsii) is an entheogenic and medicinal cactus native to the Chihuahuan desert. The psychoactive and hallucinogenic properties of peyote are principally attributed to the phenethylamine derivative mescaline. Despite the isolation ... ...

    Abstract Peyote (Lophophora williamsii) is an entheogenic and medicinal cactus native to the Chihuahuan desert. The psychoactive and hallucinogenic properties of peyote are principally attributed to the phenethylamine derivative mescaline. Despite the isolation of mescaline from peyote over 120 years ago, the biosynthetic pathway in the plant has remained undiscovered. Here, we use a transcriptomics and homology-guided gene discovery strategy to elucidate a near-complete biosynthetic pathway from l-tyrosine to mescaline. We identified a cytochrome P450 that catalyzes the 3-hydroxylation of l-tyrosine to l-DOPA, a tyrosine/DOPA decarboxylase yielding dopamine, and four substrate-specific and regiospecific substituted phenethylamine O-methyltransferases. Biochemical assays with recombinant enzymes or functional analyses performed by feeding putative precursors to engineered yeast (Saccharomyces cerevisiae) strains expressing candidate peyote biosynthetic genes were used to determine substrate specificity, which served as the basis for pathway elucidation. Additionally, an N-methyltransferase displaying broad substrate specificity and leading to the production of N-methylated phenethylamine derivatives was identified, which could also function as an early step in the biosynthesis of tetrahydroisoquinoline alkaloids in peyote.
    MeSH term(s) Mescaline/analysis ; Mescaline/chemistry ; Biosynthetic Pathways ; Phenethylamines ; Tyrosine/metabolism ; Methyltransferases/metabolism ; Cactaceae/chemistry ; Cactaceae/metabolism
    Chemical Substances Mescaline (RHO99102VC) ; Phenethylamines ; Tyrosine (42HK56048U) ; Methyltransferases (EC 2.1.1.-)
    Language English
    Publishing date 2023-09-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1088037-9
    ISSN 1365-313X ; 0960-7412
    ISSN (online) 1365-313X
    ISSN 0960-7412
    DOI 10.1111/tpj.16447
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 6071: Show issues

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  5. Article: Compartmentalization at the interface of primary and alkaloid metabolism

    Watkins, Jacinta L. / Facchini, Peter J.

    Current opinion in plant biology. 2022 Apr., v. 66

    2022  

    Abstract: Plants produce many compounds used by humans as medicines, including alkaloids of the benzylisoquinoline (BIA), monoterpene indole (MIA) and tropane classes. The biosynthetic pathways of these pharmaceutical alkaloids are complex and spatially segregated ...

    Abstract Plants produce many compounds used by humans as medicines, including alkaloids of the benzylisoquinoline (BIA), monoterpene indole (MIA) and tropane classes. The biosynthetic pathways of these pharmaceutical alkaloids are complex and spatially segregated across several tissues, cell-types and organelles. This review discusses the origin of primary metabolic inputs required by these specialized biosynthetic pathways and considers aspects relevant to their spatial organization. These factors are important for alkaloid production both in the native plants and for synthetic biology pathway reconstruction in microorganisms.
    Keywords alkaloids ; biosynthesis ; indoles ; monoterpenoids ; organelles ; plant biology ; synthetic biology
    Language English
    Dates of publication 2022-04
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1418472-2
    ISSN 1879-0356 ; 1369-5266
    ISSN (online) 1879-0356
    ISSN 1369-5266
    DOI 10.1016/j.pbi.2022.102186
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2005/717: Show issues

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  6. Article ; Online: Point-of-care ultrasound-assisted diagnosis of gastric vessel rupture in a young man presenting with chest pain: A cautionary tale.

    Yasuda, Koichi / Snelling, Peter J / Ng, Justin / Manley, Russell / Watkins, Stuart

    Australasian journal of ultrasound in medicine

    2023  Volume 26, Issue 2, Page(s) 115–117

    Abstract: We report a young male patient who presented with chest and shoulder tip pain with spontaneous intraperitoneal haemorrhage (haemoperitoneum) due to gastric vessel rupture. Point-of-care ultrasound detected abdominal free fluid, which led to a CT scan of ... ...

    Abstract We report a young male patient who presented with chest and shoulder tip pain with spontaneous intraperitoneal haemorrhage (haemoperitoneum) due to gastric vessel rupture. Point-of-care ultrasound detected abdominal free fluid, which led to a CT scan of the abdomen and reached the diagnosis. Intra-abdominal bleeding can present as referred chest or shoulder tip pain, as more commonly seen in females with pelvic pathologies. Point-of-care ultrasound may add diagnostic value with the detection of a haemoperitoneum in this context.
    Language English
    Publishing date 2023-03-05
    Publishing country Australia
    Document type Case Reports
    ZDB-ID 2843953-3
    ISSN 2205-0140 ; 1836-6864
    ISSN (online) 2205-0140
    ISSN 1836-6864
    DOI 10.1002/ajum.12338
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Improving the Corrosion Performance of Organically Coated Steel Using a Sol-Gel Overcoat.

    Watkins, Evan / Griffiths, Chris M / Richards, Calvin A J / Potts, Sarah-Jane / Batchelor, Chris / Barker, Peter / Searle, Justin / Jewell, Eifion

    Materials (Basel, Switzerland)

    2024  Volume 17, Issue 5

    Abstract: Organically coated steels are widely used in applications in which they are subjected to the natural environment and therefore require excellent corrosion resistance. Organic clearcoats are typically employed as a barrier that improves the overall ... ...

    Abstract Organically coated steels are widely used in applications in which they are subjected to the natural environment and therefore require excellent corrosion resistance. Organic clearcoats are typically employed as a barrier that improves the overall corrosion resistance; however, they are typically derived from fossil fuel-based feedstock. A more sustainable alternative could be possible using sol-gel coatings. The application of a simple tetraethoxysilane (TEOS)-based sol-gel was applied to polyurethane-coated steels using a spray coater. The concentration of TEOS was altered to produce coatings containing either 2.5% or 10%. The 10% TEOS resulted in dense, homogeneous coatings that offered a significant improvement in corrosion resistance compared to an uncoated substrate. Whereas the 2.5% TEOS coatings were inhomogeneous and porous, which indicated a limitation of concentration required to produce a uniform coating. The successful demonstration of using a simple TEOS-based coating to improve the corrosion resistance of organically coated steel highlights the potential for further investigation into the use of sol-gels for these applications.
    Language English
    Publishing date 2024-02-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma17051075
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Emergency medicine trainees' perceived barriers to training and credentialing in point-of-care ultrasound: A cross-sectional study.

    Elsayed, Tarek / Snelling, Peter J / Stirling, Erin J / Watkins, Stuart

    Australasian journal of ultrasound in medicine

    2022  Volume 25, Issue 4, Page(s) 160–165

    Abstract: Introduction: Point-of-care ultrasound (POCUS) is an important tool in emergency medicine (EM), with the Australasian College for Emergency Medicine (ACEM) recommending core modalities as part of fellowship training. In Australia, acquisition of these ... ...

    Abstract Introduction: Point-of-care ultrasound (POCUS) is an important tool in emergency medicine (EM), with the Australasian College for Emergency Medicine (ACEM) recommending core modalities as part of fellowship training. In Australia, acquisition of these skills is certified
    Methods: We performed a cross-sectional survey of EM trainees across two academic teaching hospitals in Gold Coast, Queensland, between December 2018 and January 2019, to determine the current state of training and perceived barriers to credentialing in POCUS.
    Results: Fifty-two (59%) eligible EM trainees participated. Although credentialing rates (15%) were low amongst respondents, the majority agreed that it was necessary (69%) and should form part of ACEM training (88%). Amongst these trainees, we identified the desire for increased POCUS training and several barriers including time constraints and the credentialing process itself.
    Conclusion: Although there is general agreement amongst EM trainees for POCUS credentialing, barriers such as time limitations and technical difficulties were prohibitive for many. We propose the development of an internal structured POCUS training programme within mandatory training time to address these issues.
    Language English
    Publishing date 2022-09-23
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2843953-3
    ISSN 2205-0140 ; 1836-6864
    ISSN (online) 2205-0140
    ISSN 1836-6864
    DOI 10.1002/ajum.12317
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: A survey of paediatric difficult peripheral intravenous access in the emergency department and use of point-of-care ultrasound.

    Lam, Clayton / Dunstan, Lucy / Sweeny, Amy / Watkins, Stuart / George, Shane / Snelling, Peter J

    Australasian journal of ultrasound in medicine

    2023  Volume 26, Issue 3, Page(s) 184–190

    Abstract: Introduction/purpose: Peripheral intravenous catheter (PIVC) insertion can be challenging in children, with point-of-care ultrasound (POCUS) known to increase success rates. The objective of this study was to survey how emergency department (ED) ... ...

    Abstract Introduction/purpose: Peripheral intravenous catheter (PIVC) insertion can be challenging in children, with point-of-care ultrasound (POCUS) known to increase success rates. The objective of this study was to survey how emergency department (ED) clinicians identify and escalate paediatric patients with difficult intravenous access (DIVA), specifically the use of POCUS.
    Methods: This cross-sectional study was conducted in an Australian academic mixed ED that surveyed resident medical officers (RMOs), registrars, consultants and senior paediatric nurses. A 15 multiple-choice questionnaire evaluated clinicians experience with paediatric PIVC insertion, approach to identifying and managing DIVA and the use of POCUS or other adjuncts.
    Results: Eighty clinicians (34.2% response rate) completed the survey. Poor vein palpability was rated the highest predictor of DIVA. Of the respondents, 19 consultants (86.4%), 28 registrars (90.3%) and 16 RMOs (64.0%) used POCUS as an adjunct for paediatric DIVA patients but 16 consultants (72.8%), 21 registrars (67.8%) and 20 RMOs (80.0%) would use this less than 25% of the time in clinical practice.
    Discussion: This survey suggests more clinicians to prefer using objective factors when identifying paediatric DIVA patients, rather than subjectively using gestalt, which relies on clinician experience. Whilst clearly recognised as a useful tool in our study, POCUS was used infrequently for paediatric DIVA patients.
    Conclusions: There is currently no consistent process for the identification and escalation of paediatric DIVA patients, including the use of adjuncts such as POCUS. Clinician awareness for these issues should be addressed, which should include the development of guidelines and clinician training in POCUS for PIVC insertion in children.
    Language English
    Publishing date 2023-07-13
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2843953-3
    ISSN 2205-0140 ; 1836-6864
    ISSN (online) 2205-0140
    ISSN 1836-6864
    DOI 10.1002/ajum.12353
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Heptadecanoic acid as an indicator of BCFA content in sheep fat.

    Watkins, Peter J / Frank, Damian

    Meat science

    2019  Volume 151, Page(s) 33–35

    Abstract: Branched chain fatty acids (BCFAs) are associated with 'mutton flavour' found in cooked sheep meat aroma. Three BCFAs, 4-methyloctanoic (MOA), 4-ethyloctanoic (EOA) and 4-methylnonanoic (MNA) acids, have been implicated as the main compounds responsible ... ...

    Abstract Branched chain fatty acids (BCFAs) are associated with 'mutton flavour' found in cooked sheep meat aroma. Three BCFAs, 4-methyloctanoic (MOA), 4-ethyloctanoic (EOA) and 4-methylnonanoic (MNA) acids, have been implicated as the main compounds responsible for 'mutton flavour'. Animal age can also contribute to increasing BCFA concentration. Heptadecanoic acid (C17:0 FA) also increases with animal age in sheep. Using published data, a linear association has been found to exist between C17:0 FA with MOA and MNA in sheep fat, with C17:0 FA increasing proportionally with these two compounds. No association was found between C17:0 FA and EOA. As C17:0 FA is present in sheep fat in relatively higher proportions compared to BCFAs, it has potential to be used as a proxy for MOA and MOA as well as 'mutton flavour'.
    MeSH term(s) Adipose Tissue/chemistry ; Aging ; Animals ; Cooking ; Fatty Acids/analysis ; Red Meat/analysis ; Sheep ; Taste
    Chemical Substances Fatty Acids ; margaric acid (V987Y9OZ8L)
    Language English
    Publishing date 2019-01-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 753319-6
    ISSN 1873-4138 ; 0309-1740
    ISSN (online) 1873-4138
    ISSN 0309-1740
    DOI 10.1016/j.meatsci.2019.01.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 4037: Show issues

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