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Article: Drying kinetics and thermo-economic analysis of drying hot water blanched ginger rhizomes in a hybrid composite solar dryer with heat exchanger.

Ndukwu, Macmanus Chinenye / Augustine, Edet Ben / Ugwu, Elijah / Ibeh, Mathew Imagwuike / Ekop, Inemesit / Akpan, Godwin / Udo, Anietie Effiong / Ihediwa, Victor E / Akuwueke, Leonard / Mbanasor, Jude / Abam, Fidelis

Heliyon

2023 Volume 9, Issue 2, Page(s) e13606

Abstract: This research aimed to examine the need of adding hot water blanching pre-treatment on the drying of ginger rhizomes using a hybrid solar-dryer with paraffin liquid as thermal storage infused into a copper tube to form a compact heat exchanger. Blanching ...

Abstract	This research aimed to examine the need of adding hot water blanching pre-treatment on the drying of ginger rhizomes using a hybrid solar-dryer with paraffin liquid as thermal storage infused into a copper tube to form a compact heat exchanger. Blanching duration quickened the drying rate of the ginger rhizomes and the average drying rate for blanching at 90 s, 60 s, 30 s and un-blanched ginger varied between 0.0147 kg/h to 0.0245 kg/h at a sensible heat ratio of 4.12 × 10
Language	English
Publishing date	2023-02-13
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e13606
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Fusidic Acid Resistance Determinants in Methicillin-Resistant Staphylococcus aureus Isolated in Kuwait Hospitals.

Boloki, Halimah A / Al-Musaileem, Wasmiyah F / AlFouzan, Wadha / Verghese, Tina / Udo, Edet E

Medical principles and practice : international journal of the Kuwait University, Health Science Centre

2021 Volume 30, Issue 6, Page(s) 542–549

Abstract: ... The minimum inhibitory concentration (MIC) of FA was tested with E-test strips. Genetic determinants of FA were determined by PCR and ...

Abstract	Objective: The aim of this study was to investigate the genetic determinants of fusidic acid (FA) resistance in MRSA isolated from patients in Kuwait hospitals. Methods: The minimum inhibitory concentration (MIC) of FA was tested with E-test strips. Genetic determinants of FA were determined by PCR and DNA microarray. Staphylococcal protein A gene (spa) typing and DNA microarray analysis were used to study their genetic backgrounds. Results: The FA MIC ranged from 2 mg/L to >256 mg/L. Of the 97 isolates, 79 (81.4%) harbored fusC, 14 isolates harbored fusA mutations (fusA), and 4 isolates harbored fusB. Isolates with fusA mutations expressed high FA MIC (MIC >256 mg/L), whereas those with fusC and fusB expressed low FA MIC (MIC 2-16 mg/L). The isolates belonged to 23 spa types and 12 clonal complexes (CCs). The major spa types were t688 (n = 25), t311 (n = 14), t860 (n = 8), and t127 (n = 6) which constituted 54.6% of the isolates. The 12 CCs were CC1, CC5, CC8, CC15, CC22, CC80, CC88, and CC97 with CC5 (45.6%) and CC97 (13.2%) as the dominant CCs. Conclusions: The MRSA isolates belonged to diverse genetic backgrounds with the majority carrying the fusC resistance determinants. The high prevalence of FA resistance belonging to diverse genetic backgrounds warrants a review of FA usage in the country to preserve its therapeutic benefits.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Drug Resistance, Bacterial/genetics ; Fusidic Acid/pharmacology ; Humans ; Kuwait/epidemiology ; Methicillin-Resistant Staphylococcus aureus/genetics ; Microbial Sensitivity Tests ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction ; Staphylococcal Infections/drug therapy ; Staphylococcal Infections/epidemiology ; Staphylococcus/drug effects ; Staphylococcus/genetics
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Fusidic Acid (59XE10C19C)
Language	English
Publishing date	2021-07-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	645108-1
ISSN	1423-0151 ; 1011-7571
ISSN (online)	1423-0151
ISSN	1011-7571
DOI	10.1159/000518408
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Article: Methicillin-Resistant

Alfouzan, Wadha A / Boswihi, Samar S / Udo, Edet E

Microorganisms

2023 Volume 12, Issue 1

Abstract: Methicillin- ... ...

Abstract	Methicillin-resistant
Language	English
Publishing date	2023-12-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms12010017
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Article: Demographics, Cost, and Sustainability of Haemodialysis among End-Stage Kidney Disease Patients in Southern Nigeria: A Single-Center Study.

Akpan, Effiong E / Ekrikpo, Udeme E / Effa, Emmanuel Edet / Udo, Aniema I A / Umoh, Victor A

Nigerian medical journal : journal of the Nigeria Medical Association

2020 Volume 61, Issue 6, Page(s) 307–311

Abstract: Context: Access to chronic hemodialysis for patients with end-stage kidney disease has improved over the years. However, it is unclear if this has resulted in lower cost and improved dialysis vintage.: Aim: We aimed to assess the demographics, cost ... ...

Abstract	Context: Access to chronic hemodialysis for patients with end-stage kidney disease has improved over the years. However, it is unclear if this has resulted in lower cost and improved dialysis vintage. Aim: We aimed to assess the demographics, cost implication, and sustainability of maintenance hemodialysis in our cohort of end-stage kidney disease (ESKD) patients. Methods: Retrospective descriptive study of ESKD patients on maintenance HD from 2014 to 2018 using hemodialysis records. Time-to-HD discontinuation and reasons for discontinuation were recorded. Using Kaplan-Meier graphs, the time-to-dialysis discontinuation experience of the cohort was shown. Log-rank test was used to compare the experience between both genders. Univariable and multivariable Cox proportional hazard models were built to identify independent associations with time-to-dialysis discontinuation. Results: Over the 5-year period, 702 individuals initiated HD, males were older than females, the complete cohort contributed 65,714 person-days to the study and the median time-to-HD discontinuation was 10 days (interquartile range, 2-42). Females had a shorter time to HD discontinuation (8 days [1-32 days]) compared to males (11 days [2-48 days]). Only 28.5%, 15.3% and 8.3% of the patients had HD beyond 30, 90, and 180 days, respectively. About 128 (18.2%) had thrice-weekly HD. Most sustained the treatment for the 1 Conclusion: Most patients cannot sustain HD beyond a few weeks for financial reasons. Several cost containment strategies need to be deployed to bring down the cost of care.
Language	English
Publishing date	2020-12-19
Publishing country	Nigeria
Document type	Journal Article
ZDB-ID	424429-1
ISSN	0300-1652
ISSN	0300-1652
DOI	10.4103/nmj.NMJ_106_20
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Article ; Online: Antimicrobial resistance and virulence determinants in coagulase-negative staphylococci isolated mainly from preterm neonates.

Aishah Al-Haqan / Samar S Boswihi / Seema Pathan / Edet E Udo

PLoS ONE, Vol 15, Iss 8, p e

2020 Volume 0236713

Abstract: Coagulase-negative staphylococci (CoNS) are the most common isolates from blood culture in neonates resulting in high mortality and morbidity. This study investigated CoNS obtained from blood cultures of neonates for antibiotic resistance and virulence ... ...

Abstract	Coagulase-negative staphylococci (CoNS) are the most common isolates from blood culture in neonates resulting in high mortality and morbidity. This study investigated CoNS obtained from blood cultures of neonates for antibiotic resistance and virulence factors, and possible association with inflammatory response (C-reactive protein). A total of 93 CoNS isolates were collected from 76 blood cultures of neonates at the Maternity hospital in Kuwait in a six-month period and investigated for susceptibility to antibiotics, carriage of staphylococcal cassette chromosome mec (SCCmec), and virulence-associated genes. The 93 CoNS isolates consisted of S. epidermidis (76; 81.7%), S. capitis (12; 12.9%), S. hominis (2; 2.1%), S. warneri (2; 2.1%) and S. haemolyticus (1; 1.0%). Eighty-six (92.4%) of the isolates were resistant to cefoxitin (MR-CoNS) while 49 (52.7%) expressed multi-antibiotic resistance. The methicillin-resistant isolates (MR-CoNS) carried SCCmec III, SCCmec IVa and four combinations of SCCmec types including SCCmec types I+IVa (one S. warneri and 25 S. epidermidis isolates), types I+III (one S. epidermidis isolate), types III+IVa (six S. epidermidis isolates) and types I+III+IVa (one S. epidermidis isolate). The most common virulence-related genes were icaC, seb, arc detected in 69.7%, 60.5%, 40.8% of the isolates respectively. Two isolates were positive for tst1. No association between C-reactive protein and antibiotic resistance or virulence factors was established. This study revealed that S. epidermidis carrying different SCCmec genetic elements, was the dominant CoNS species isolated from neonatal blood cultures with 90.3% and 36.6% of the isolates positive for genes for biofilm and ACME production respectively.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Dominance of community-associated methicillin-resistant Staphylococcus aureus clones in a maternity hospital.

Edet E Udo / Noura Al-Sweih

PLoS ONE, Vol 12, Iss 6, p e

2017 Volume 0179563

Abstract: Methicillin- resistant Staphylococcus aureus (MRSA) is a major pathogen causing healthcare- and community- acquired infections. The purpose of this study was to characterize MRSA isolated at the Maternity Hospital between 2006 and 2011 for their genetic ... ...

Abstract	Methicillin- resistant Staphylococcus aureus (MRSA) is a major pathogen causing healthcare- and community- acquired infections. The purpose of this study was to characterize MRSA isolated at the Maternity Hospital between 2006 and 2011 for their genetic relatedness.The MRSA isolates were investigated using a combination of antibiogram, Staphylococcal chromosome cassette mec (SCCmec) and spa typing to determine their relatedness to MRSA isolated in other Kuwait hospitals. The isolates were also investigated for the carriage of genes for Pantone valentine Leukocidin (PVL).A total of 103 MRSA obtained from 64 neonates, 17 adult patients and 12 healthcare workers. The isolates were resistant to Kanamycin (46.6%), gentamicin (40.8%), trimethoprim (32%), ciprofloxacin (22.3%), fusidic acid (16.5%), tetracycline (19.4%), erythromycin (15.5%), clindamycin (15.5%), streptomycin (11.6%) high-level mupirocin (2.9%) and chloramphenicol (0.9%). Twenty (19.4%) of the isolates were multiresistant. Thirty-one (30.0%) isolates were positive for PVL. Molecular typing revealed the presence of 11 clonal complexes and 23 clones with ST5-V-t002, (N = 22), ST22-IV-t223 (N = 18), ST22-IV-t852 (N = 10), ST80-IV-t044 (N = 7), ST5-V-t688 (N = 5), ST772-V-t657 (N = 5) and ST239-III-t860 (N = 4) constituting 66.9% of the isolates. Other clones were isolated sporadically. The number of MRSA isolates increased from two in 2006 to 22 in 2011 with a peak of 43 in 2008.The study revealed a high prevalence of community-associated MRSA Maternity hospital. The MRSA population consisted of known strains, such as ST239-III-t680, ST22-IV-t223/t852 and ST80-IV-t044, that were reported previously in Kuwait and novel strains such as ST5-V-t002, and several sporadic strains obtained for the first time in the Maternity hospital. This study has provided an initial data which will serve as a platform for future comparative studies on the distribution of MRSA clones in the Maternity hospital in Kuwait.
Keywords	Medicine ; R ; Science ; Q
Subject code	360
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Drying kinetics and thermo-economic analysis of drying hot water blanched ginger rhizomes in a hybrid composite solar dryer with heat exchanger

Ndukwu, Macmanus Chinenye / Augustine, Edet Ben / Ugwu, Elijah / Ibeh, Mathew Imagwuike / Ekop, Inemesit / Akpan, Godwin / Udo, Anietie Effiong / Ihediwa, Victor E. / Akuwueke, Leonard / Mbanasor, Jude / Abam, Fidelis

Heliyon. 2023 Feb., v. 9, no. 2 p.e13606-

2023

Abstract	This research aimed to examine the need of adding hot water blanching pre-treatment on the drying of ginger rhizomes using a hybrid solar-dryer with paraffin liquid as thermal storage infused into a copper tube to form a compact heat exchanger. Blanching duration quickened the drying rate of the ginger rhizomes and the average drying rate for blanching at 90 s, 60 s, 30 s and un-blanched ginger varied between 0.0147 kg/h to 0.0245 kg/h at a sensible heat ratio of 4.12 × 10⁻⁵ to 2.53 × 10⁻³. The optimal drying rate varied from 0.01161 kg/h to 0.0263 kg/h for all treatment at a collector temperature range of 39.5 °C-40.5 °C and collector efficiency range of 14.3%-30%. The logarithmic model better predicted the drying kinetics of un-blanched and blanching for 30 s with an R² value of 0.9875 and 0.97247 respectively while the modified Henderson and Pabis model better predicted drying of blanched ginger rhizomes at 60 s and 90 s with R² values of 0.96252 and 0.98188 respectively. Using the hybrid solar dryer instead of artificial dryers with fossil energy sources can save about $75.731 to $757.31 of the running cost as the usage increased from 10 to 100%. The payback period decreased from 2.88 years to 0.31 years as the rate of usage increased from 10 to 100%. Using the presented solar dryer instead of coal, diesel or grid base electricity can prevent 15.96 to 186, 7.62 tones of CO₂ from entering the atmosphere. The earned carbon credit if the dryer is to be powered by coal, diesel or grid base electricity were $ $6245364, $27080.52, and $231.45 per year respectively which can be used to compensate other non-renewable energy sources deployed within an energy enterprise.
Keywords	business enterprises ; carbon dioxide ; carbon markets ; coal ; economic analysis ; electricity ; energy ; ginger ; heat ; heat exchangers ; liquids ; models ; nonrenewable resources ; solar dryers ; temperature ; Agricultural products ; Decarbonisation ; Drying kinetics ; Hot water blanching ; Solar thermal storage
Language	English
Dates of publication	2023-02
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e13606
Database	NAL-Catalogue (AGRICOLA)

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Article: Diversity of clonal complex 22 methicillin-resistant

Boswihi, Samar S / Verghese, Tina / Udo, Edet E

Frontiers in microbiology

2022 Volume 13, Page(s) 970924

Abstract: CC22-MRSA is a major MRSA lineage that is widely reported globally. To characterize CC22-MRSA for trends in antibiotic resistance and emergence of variants, a total of 636 CC22 isolates identified by DNA microarray in 2016 ( ...

Abstract	CC22-MRSA is a major MRSA lineage that is widely reported globally. To characterize CC22-MRSA for trends in antibiotic resistance and emergence of variants, a total of 636 CC22 isolates identified by DNA microarray in 2016 (
Language	English
Publishing date	2022-08-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2022.970924
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Article ; Online: Description of Methicillin-Susceptible Staphylococcus aureus Clonal Complex 30 Related to the Pandemic Phage Type 80/81 Isolated from Patients in Three Tertiary Hospitals in Jos, North Central Nigeria.

Essien, Unyime C / Boswihi, Samar S / Agbakoba, Nneka R / Udo, Edet E

Medical principles and practice : international journal of the Kuwait University, Health Science Centre

2022 Volume 31, Issue 3, Page(s) 269–275

Abstract: Objective: The prevalence of phage 80/81 Staphylococcus aureus strains, the pandemic strains that were dominant in the 1950s, had declined in the 1960s and 1970s. However, these strains have reemerged in some countries in recent years. This study ... ...

Abstract	Objective: The prevalence of phage 80/81 Staphylococcus aureus strains, the pandemic strains that were dominant in the 1950s, had declined in the 1960s and 1970s. However, these strains have reemerged in some countries in recent years. This study investigated the antibacterial resistance, virulence, and the genetic backgrounds of CC30-MSSA isolates obtained from patients in three tertiary hospitals. Materials and methods: Twenty-two CC30-MSSA isolates cultured from different clinical samples were investigated using antibiotic sensitivity testing, spa typing, multilocus sequence typing, and DNA microarray analysis. Results: All 22 isolates were susceptible to vancomycin (MIC ≤2 μg/mL), teicoplanin (MIC ≤2 μg/mL), and cefoxitin but were resistant to penicillin G (n = 22; 100.0%), tetracycline (n = 12; 54.5%), ciprofloxacin (n = 15; 68.2%), cadmium acetate (n = 22; 100%), mercuric chloride (n = 13; 59.1%), and ethidium bromide (n = 3; 13.6%). The isolates belonged to sequence type, ST30, and five spa types: t012 (n = 12; 54.5%), t019 (n = 5; 22.7%), t017 (n = 2; 9.1%), t037 (n = 2; 9.1%), and t318 (n = 1; 4.5%). All 22 isolates were positive for agrIII, cap8, clfA, clfB, icaA, icaC, icaD, cna, and staphylococcal enterotoxin gene clusters (seg, sei, sem, sen, seo, seu). Eight isolates carried lukS-PV and lukF-PV that code for Panton-Valentine leukocidin. Conclusion: The current CC30-MSSA isolates share phenotypic and genotypic characteristics with the pandemic phage 80/81 isolates that were common in the 1950s and 1960s. Continued surveillance is recommended to keep abreast of the changing epidemiology of S. aureus causing healthcare and community-associated infections.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacteriophages/genetics ; Humans ; Methicillin ; Methicillin-Resistant Staphylococcus aureus/genetics ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Nigeria/epidemiology ; Pandemics ; Staphylococcal Infections/epidemiology ; Staphylococcus aureus/genetics ; Tertiary Care Centers
Chemical Substances	Anti-Bacterial Agents ; Methicillin (Q91FH1328A)
Language	English
Publishing date	2022-05-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	645108-1
ISSN	1423-0151 ; 1011-7571
ISSN (online)	1423-0151
ISSN	1011-7571
DOI	10.1159/000524755
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Article ; Online: Shifts in the Clonal Distribution of Methicillin-Resistant Staphylococcus aureus in Kuwait Hospitals

Samar S Boswihi / Edet E Udo / Noura Al-Sweih

PLoS ONE, Vol 11, Iss 9, p e

1992-2010.

2016 Volume 0162744

Abstract: BACKGROUND:As the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) is constantly changing globally, determining the prevailing MRSA clones in a local healthcare facility is important for better management of infections. This study ... ...

Abstract	BACKGROUND:As the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) is constantly changing globally, determining the prevailing MRSA clones in a local healthcare facility is important for better management of infections. This study investigated clonal composition and distribution of MRSA isolates in Kuwait's hospitals using a combination of molecular typing methods. MATERIALS AND METHODS:In total, 400 non-repeat MRSA isolates were obtained between 1992 and 2010 in 13 public hospitals and were characterized using antibiogram, SCCmec typing, spa typing, and multilocus-sequence typing. Clonal assignment and detection of virulence factors and antibiotic resistance genes were performed by DNA microarray. RESULTS:The isolates were resistant to kanamycin (74.2%), erythromycin (69.5%), tetracycline (66.7%), gentamicin (61%), ciprofloxacin, (61%), fusidic acid (53.5%), clindamycin (41.5%), high-level mupirocin resistance (5.2%) and carried aphA3, aacA-aphD, ermA, ermC, mupA, tetK, tetM, fusC and far1. Molecular typing revealed 31 different MRSA clones consisting of ST239-MRSA-III (52.2%), ST22-MRSA-IV (9.2%), ST80-MRSA-IV (7.5%), ST5-MRSA-II/IV/V/VI (6.5%), ST30-MRSA-IV (3.5%), ST241-MRSA-III (2.7%), ST6-MRSA-IV (2.2%), ST36-MRSA-II (2%) and ST772-MRSA-V (1.75%). The isolates differed in the carriage of genes for enterotoxins, Panton-Valentine leukocidin (PVL), toxic shock syndrome toxin (tst-1), arginine catabolic mobile element (ACME) and exfoliative toxins. The number of clones increased from one (ST239-III-t037) in 1992 to 30 in 2010 including ST8-IV-t008 [PVL+] [ACME+] (USA300), ST772-V (Bengal Bay clone) and ST2816 identified for the first time in Kuwait. CONCLUSION:The study revealed that the MRSA isolates belonged to diverse clones that changed in numbers and diversity overtime. Although ST239-MRSA-III, a healthcare-associated clone remained the dominant MRSA clone overtime, the newly emerged clones consisted mostly of community-associated.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2016-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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