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Article: The endocannabinoid system in pain and inflammation: Its relevance to rheumatic disease.

Barrie, Nicola / Manolios, Nicholas

2017 Volume 4, Issue 3, Page(s) 210–218

Abstract: Pain is the most common manifestation of both acute and chronic inflammation that often challenges patients with rheumatic disease. Simply, we attribute this to local joint changes of pH in joints, the formation of radicals, enhanced joint pressure, or ... ...

Abstract	Pain is the most common manifestation of both acute and chronic inflammation that often challenges patients with rheumatic disease. Simply, we attribute this to local joint changes of pH in joints, the formation of radicals, enhanced joint pressure, or cytokine release acting on local nerves to produce pain. However, there is a more complex interplay of interactions between cytokines, mediators of inflammation, and ion channels that influence the final immune response and our perception of pain. Endocannabinoids, a group of less well-known endogenous bioactive lipids, have such manifold immunomodulatory effects able to influence both inflammation and pain. In this review, we overview the endocannabinoid system, its role in pain, inflammation, and immune regulation, and highlight the emerging challenges and therapeutic hopes.
Language	English
Publishing date	2017-09-01
Publishing country	Turkey
Document type	Journal Article ; Review
ZDB-ID	2873727-1
ISSN	2148-4279 ; 2147-9720
ISSN (online)	2148-4279
ISSN	2147-9720
DOI	10.5152/eurjrheum.2017.17025
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Article ; Online: Respiratory Outcomes of Firefighter Exposures in the Fort McMurray Fire: A Cohort Study From Alberta Canada.

Cherry, Nicola / Barrie, James R / Beach, Jeremy / Galarneau, Jean-Michel / Mhonde, Trish / Wong, Eric

Journal of occupational and environmental medicine

2021 Volume 63, Issue 9, Page(s) 779–786

Abstract: Objective: Determine effects on respiratory health of firefighters attending a catastrophic wildfire.: Methods: Within the Alberta Administrative Health Database, we identified five community-based controls for each firefighter in a cohort of 1234 ... ...

Abstract	Objective: Determine effects on respiratory health of firefighters attending a catastrophic wildfire. Methods: Within the Alberta Administrative Health Database, we identified five community-based controls for each firefighter in a cohort of 1234 deployed to the 2016 Fort McMurray fire. Spirometry records were identified and a stratified sample assessed clinically. We estimated PM2.5 particles exposure. Results: Firefighters had an increased risk of asthma consultation post-fire (OR new onset asthma = 2.56; 95%CI 1.75 to 3.74). Spirometry showed decreased FEV1 and FVC with increasing exposure. In the clinical assessment, 20% had a positive MCT and 21% BWT. Those with ongoing fire-related symptoms had a higher concurrence of positive MCT and BWT (OR = 4.35; 95%CI 1.11 to 17.12). Lower diffusion capacity related to higher exposure. Conclusions: Massive exposures during a wildfire are associated with non-resolving airways damage.
MeSH term(s)	Alberta/epidemiology ; Cohort Studies ; Firefighters ; Fires ; Humans ; Occupational Exposure/adverse effects ; Occupational Exposure/analysis ; Wildfires
Language	English
Publishing date	2021-09-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1223932-x
ISSN	1536-5948 ; 1076-2752
ISSN (online)	1536-5948
ISSN	1076-2752
DOI	10.1097/JOM.0000000000002286
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Article ; Online: Autism spectrum disorders in boys at a major UK hemophilia center: prevalence and risk factors.

Bladen, Melanie / Thorpe, Nicola / Ridout, Deborah / Barrie, Alpha / McGibbon, Emma / Mance, Abigail / Watson, Lucy / Main, Eleanor

Research and practice in thrombosis and haemostasis

2022 Volume 7, Issue 1, Page(s) 100013

Abstract: Background: Autism spectrum disorders (ASDs) are diagnosed by social communication difficulties strong, narrow interests, and repetitive stereotyped behavior. An apparently-elevated prevalence of ASD at a major UK hemophilia center warranted ... ...

Abstract	Background: Autism spectrum disorders (ASDs) are diagnosed by social communication difficulties strong, narrow interests, and repetitive stereotyped behavior. An apparently-elevated prevalence of ASD at a major UK hemophilia center warranted investigation. Objectives: To screen boys with hemophilia for difficulties in social communication and executive function and identify the prevalence and risk factors for ASD. Methods: Parents of boys with hemophilia aged 5 to 16 years completed the Social Communication Questionnaire, Children's Communication Checklist, and the Behavior Rating Inventory of executive function. Prevalence and potential risk factors for ASD were evaluated. Boys with an existing diagnosis of ASD did not complete questionnaires, but were included in the prevalence analysis. Results: Negative scores on all 3 questionnaires were observed for 60 of 79 boys. Positive scores on 1, 2, and 3 questionnaires were seen in 12 of 79, 3 of 79, and 4 of 79 boys, respectively. In addition to the 11 of 214 boys with a prior ASD diagnosis, 3 further boys were diagnosed with ASD, yielding a prevalence of 14 (6.5%) of 214, greater than that of boys in the UK general population. Premature birth was linked to having ASD, but did not fully explain the increased prevalence with more boys born <37 weeks scoring positively on the Social Communications Questionnaire and Children's Communication Checklist compared with those born at term. Conclusion: This study identified an increased prevalence of ASD at 1 UK hemophilia center. Prematurity was identified as a risk factor but did not fully explain the higher prevalence of ASD. Further investigation in the wider national/global hemophilia communities is warranted to determine whether this is an isolated finding.
Language	English
Publishing date	2022-12-13
Publishing country	United States
Document type	Journal Article
ISSN	2475-0379
ISSN (online)	2475-0379
DOI	10.1016/j.rpth.2022.100013
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Article ; Online: Endocannabinoids in arthritis: current views and perspective.

Barrie, Nicola / Kuruppu, Vindhya / Manolios, Emmanuel / Ali, Marina / Moghaddam, Minoo / Manolios, Nicholas

International journal of rheumatic diseases

2017 Volume 20, Issue 7, Page(s) 789–797

Abstract: Preclinical and clinical studies using cannabis-based therapy have been shown to provide both analgesia and anti-inflammatory effects, with an overall alleviation of clinical symptoms in animal models of arthritis, highlighting its promising therapeutic ... ...

Abstract	Preclinical and clinical studies using cannabis-based therapy have been shown to provide both analgesia and anti-inflammatory effects, with an overall alleviation of clinical symptoms in animal models of arthritis, highlighting its promising therapeutic application for humans. Despite this, the development of cannabis-based therapeutics remains in its infancy, with further investigation into its efficacy and safety profile in patients still required. This synopsis reviews the various components of the endocannabinoid system in health and disease and their potential as therapeutic targets.
Language	English
Publishing date	2017-07
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2426924-4
ISSN	1756-185X ; 1756-1841
ISSN (online)	1756-185X
ISSN	1756-1841
DOI	10.1111/1756-185X.13146
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Article ; Online: Automatic data extraction from 24 hour blood pressure measurement reports of a large multicenter clinical trial.

Nolde, Janis M / Mian, Ajmal / Schlaich, Luca / Chan, Justine / Lugo-Gavidia, Leslie Marisol / Barrie, Nicola / Gopal, Vishal / Hillis, Graham S / Chow, Clara K / Schlaich, Markus P

Computer methods and programs in biomedicine

2021 Volume 214, Page(s) 106588

Abstract: Background and objectives: Ambulatory blood pressure monitoring (ABPM) is usually reported in descriptive values such as circadian averages and standard deviations. Making use of the original, individual blood pressure measurements may be advantageous, ... ...

Abstract	Background and objectives: Ambulatory blood pressure monitoring (ABPM) is usually reported in descriptive values such as circadian averages and standard deviations. Making use of the original, individual blood pressure measurements may be advantageous, particularly for research purposes, as this increases the flexibility of the analytical process, enables alternative statistical analyses and provide novel insights. Here we describe the development of a new multistep, hierarchical data extraction algorithm to collect raw data from .pdf reports and text files as part of a large multi-center clinical study. Methods: Original reports were saved in a nested file system, from which they were automatically extracted, read and saved into databases with custom made programs written in Python 3. Data were further processed, cleaned and relevant descriptive statistics such as averages and standard deviations calculated according to a variety of definitions of day- and night-time. Additionally, data control mechanisms for manual review of the data and programmatic auto-detection of extraction errors was implemented as part of the project. Results: The developed algorithm extracted 97% of the data automatically, the missing data consisted mostly of reports that were saved incorrectly or not formatted in the specified way. Manual checks comparing samples of the extracted data to original reports indicated a high level of accuracy of the extracted data, no errors introduced due to flaws in the extraction software were detected in the extracted dataset. Conclusions: The developed multistep, hierarchical data extraction algorithm facilitated collection from different file formats and paired with database cleaning and data processing steps led to an effective and accurate assembly of raw ABPM data for further and adjustable analyses. Manual work was minimized while data quality was ensured with standardized, reproducible procedures.
MeSH term(s)	Algorithms ; Blood Pressure ; Blood Pressure Monitoring, Ambulatory ; Databases, Factual ; Software
Language	English
Publishing date	2021-12-17
Publishing country	Ireland
Document type	Journal Article ; Multicenter Study
ZDB-ID	632564-6
ISSN	1872-7565 ; 0169-2607
ISSN (online)	1872-7565
ISSN	0169-2607
DOI	10.1016/j.cmpb.2021.106588
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Article ; Online: Genome editing reveals that pSCL4 is required for chromosome linearity in

Gomez-Escribano, Juan Pablo / Algora Gallardo, Lis / Bozhüyük, Kenan A J / Kendrew, Steven G / Huckle, Benjamin D / Crowhurst, Nicola A / Bibb, Mervyn J / Collis, Andrew J / Micklefield, Jason / Herron, Paul R / Wilkinson, Barrie

Microbial genomics

2021 Volume 7, Issue 11

Abstract: Streptomyces ... ...

Abstract	Streptomyces clavuligerus
MeSH term(s)	Chromosomes ; Gene Editing/methods ; Plasmids/genetics ; Streptomyces/genetics
Language	English
Publishing date	2021-11-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2835258-0
ISSN	2057-5858 ; 2057-5858
ISSN (online)	2057-5858
ISSN	2057-5858
DOI	10.1099/mgen.0.000669
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Article ; Online: Matched Case-Control Comparative Study of Laparoscopic Versus Open Pancreaticoduodenectomy for Malignant Lesions.

Khaled, Yazan S / Fatania, Kavi / Barrie, Jenifer / De Liguori, Nicola / Deshpande, Rahul / O'Reilly, Derek A / Ammori, Basil J

Surgical laparoscopy, endoscopy & percutaneous techniques

2017 Volume 28, Issue 1, Page(s) 47–51

Abstract: Introduction: Advances in surgical technologies allowed safe laparoscopic pancreaticoduodenectomy (LPD). The aim of this study is to compare the oncologic outcomes of LPD to open pancreaticoduodenectomy (OPD) in terms of safety and recurrence rate.: ... ...

Abstract	Introduction: Advances in surgical technologies allowed safe laparoscopic pancreaticoduodenectomy (LPD). The aim of this study is to compare the oncologic outcomes of LPD to open pancreaticoduodenectomy (OPD) in terms of safety and recurrence rate. Materials and methods: A cohort of 30 patients were matched for age, sex, American Society of Anaesthesiologists, tumor size, pancreatic duct diameter, and histopathologic diagnosis on a 1:1 basis (15 LPD, 15 OPD). Comparison between groups was performed on intention-to-treat basis. Survival following resection was compared using the Kaplan-Meier survival analysis. Results: The median operating time for LPD group was longer than for OPD group (470 vs. 310 min; P=0.184). However, estimated blood loss (300 vs. 620 mL; P=0.023), high dependency unit stay (2.0 vs. 6.0 d; P=0.013) and postoperative hospital stay (9.0 vs. 17.4 d; P=0.017) were significantly lower in the LPD group. There was no significant difference in postoperative rates of morbidity (40% vs. 67%; P=0.431) and mortality (0% vs. 6.7%; P=0.99). The surgical resection margins R0 status (87% vs. 73%; P=0.79) and the number of lymph nodes (18 vs. 20; P=0.99) in the resected specimens were comparable between the 2 groups. There was no significant difference in overall survival outcomes. Conclusions: In selected patients, the laparoscopic approach to pancreaticoduodenectomy in the hands of the experienced offers advantages over open surgery without compromising the oncologic resection.
MeSH term(s)	Adult ; Aged ; Case-Control Studies ; Disease-Free Survival ; Female ; Humans ; Kaplan-Meier Estimate ; Laparoscopy/methods ; Laparoscopy/mortality ; Laparotomy/methods ; Laparotomy/mortality ; Length of Stay ; Male ; Middle Aged ; Operative Time ; Pancreatic Neoplasms/mortality ; Pancreatic Neoplasms/pathology ; Pancreatic Neoplasms/surgery ; Pancreaticoduodenectomy/methods ; Pancreaticoduodenectomy/mortality ; Prognosis ; Risk Assessment ; Statistics, Nonparametric ; Survival Rate ; Treatment Outcome ; United Kingdom
Language	English
Publishing date	2017-02-17
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	1475108-2
ISSN	1534-4908 ; 1530-4515 ; 1051-7200
ISSN (online)	1534-4908
ISSN	1530-4515 ; 1051-7200
DOI	10.1097/SLE.0000000000000381
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Article ; Online: Serum amyloid A receptor blockade and incorporation into high-density lipoprotein modulates its pro-inflammatory and pro-thrombotic activities on vascular endothelial cells.

Chami, Belal / Barrie, Nicola / Cai, Xiaoping / Wang, Xiaosuo / Paul, Moumita / Morton-Chandra, Rebecca / Sharland, Alexandra / Dennis, Joanne M / Freedman, Saul B / Witting, Paul K

International journal of molecular sciences

2015 Volume 16, Issue 5, Page(s) 11101–11124

Abstract: The acute phase protein serum amyloid A (SAA), a marker of inflammation, induces expression of pro-inflammatory and pro-thrombotic mediators including ICAM-1, VCAM-1, IL-6, IL-8, MCP-1 and tissue factor (TF) in both monocytes/macrophages and endothelial ... ...

Abstract	The acute phase protein serum amyloid A (SAA), a marker of inflammation, induces expression of pro-inflammatory and pro-thrombotic mediators including ICAM-1, VCAM-1, IL-6, IL-8, MCP-1 and tissue factor (TF) in both monocytes/macrophages and endothelial cells, and induces endothelial dysfunction-a precursor to atherosclerosis. In this study, we determined the effect of pharmacological inhibition of known SAA receptors on pro-inflammatory and pro-thrombotic activities of SAA in human carotid artery endothelial cells (HCtAEC). HCtAEC were pre-treated with inhibitors of formyl peptide receptor-like-1 (FPRL-1), WRW4; receptor for advanced glycation-endproducts (RAGE), (endogenous secretory RAGE; esRAGE) and toll-like receptors-2/4 (TLR2/4) (OxPapC), before stimulation by added SAA. Inhibitor activity was also compared to high-density lipoprotein (HDL), a known inhibitor of SAA-induced effects on endothelial cells. SAA significantly increased gene expression of TF, NFκB and TNF and protein levels of TF and VEGF in HCtAEC. These effects were inhibited to variable extents by WRW4, esRAGE and OxPapC either alone or in combination, suggesting involvement of endothelial cell SAA receptors in pro-atherogenic gene expression. In contrast, HDL consistently showed the greatest inhibitory action, and often abrogated SAA-mediated responses. Increasing HDL levels relative to circulating free SAA may prevent SAA-mediated endothelial dysfunction and ameliorate atherogenesis.
MeSH term(s)	Apolipoprotein A-I/metabolism ; Cells, Cultured ; Endothelial Cells/cytology ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Enzyme-Linked Immunosorbent Assay ; Gene Expression Regulation/drug effects ; Humans ; Immunohistochemistry ; Lipoproteins, HDL/isolation & purification ; Lipoproteins, HDL/pharmacology ; NF-kappa B/genetics ; NF-kappa B/metabolism ; Peptides/pharmacology ; Phosphatidylcholines/pharmacology ; Receptor for Advanced Glycation End Products/genetics ; Receptor for Advanced Glycation End Products/metabolism ; Receptors, Formyl Peptide/chemistry ; Receptors, Formyl Peptide/metabolism ; Receptors, Lipoxin/chemistry ; Receptors, Lipoxin/metabolism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Recombinant Proteins/pharmacology ; Serum Amyloid A Protein/antagonists & inhibitors ; Serum Amyloid A Protein/metabolism ; Serum Amyloid A Protein/pharmacology ; Thromboplastin/genetics ; Thromboplastin/metabolism ; Tumor Necrosis Factor-alpha/analysis ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/metabolism ; Vascular Endothelial Growth Factor A/metabolism
Chemical Substances	Apolipoprotein A-I ; FPR2 protein, human ; Lipoproteins, HDL ; NF-kappa B ; Peptides ; Phosphatidylcholines ; Receptor for Advanced Glycation End Products ; Receptors, Formyl Peptide ; Receptors, Lipoxin ; Recombinant Proteins ; Serum Amyloid A Protein ; Tumor Necrosis Factor-alpha ; Vascular Endothelial Growth Factor A ; oxidized-L-alpha-1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine ; Thromboplastin (9035-58-9)
Language	English
Publishing date	2015-05-15
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms160511101
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Article ; Online: CAR T-cell therapy induces a high rate of prolonged remission in relapsed primary CNS lymphoma: Real-life results of the LOC network.

Choquet, Sylvain / Soussain, Carole / Azar, Nabih / Morel, Véronique / Metz, Carole / Ursu, Renata / Waultier-Rascalou, Agathe / di Blasi, Roberta / Houot, Roch / Souchet, Laetitia / Roos-Weil, Damien / Uzunov, Madalina / Quoc, Stéphanie Nguyen / Jacque, Nathalie / Boussen, Inès / Gauthier, Nicolas / Ouzegdouh, Maya / Blonski, Marie / Campidelli, Arnaud /

Ahle, Guido / Guffroy, Blandine / Willems, Lise / Corvilain, Emilie / Barrie, Maryline / Alcantara, Marion / le Garff-Tavernier, Magali / Psimaras, Dimitri / Weiss, Nicolas / Baron, Marine / Bravetti, Clotilde / Hoang-Xuan, Khê / Davi, Frédéric / Shor, Natalia / Alentorn, Agusti / Houillier, Caroline

American journal of hematology

2024

Abstract: The prognosis of relapsed primary central nervous system lymphoma (PCNSL) remains dismal. CAR T-cells are a major contributor to systemic lymphomas, but their use in PCNSL is limited. From the LOC network database, we retrospectively selected PCNSL who ... ...

Abstract	The prognosis of relapsed primary central nervous system lymphoma (PCNSL) remains dismal. CAR T-cells are a major contributor to systemic lymphomas, but their use in PCNSL is limited. From the LOC network database, we retrospectively selected PCNSL who had leukapheresis for CAR-T cells from the third line of treatment, and, as controls, PCNSL treated with any treatment, at least in the third line and considered not eligible for ASCT. Twenty-seven patients (median age: 68, median of three previous lines, including ASCT in 14/27) had leukapheresis, of whom 25 received CAR T-cells (tisa-cel: N = 16, axi-cel: N = 9) between 2020 and 2023. All but one received a bridging therapy. The median follow-up after leukapheresis was 20.8 months. The best response after CAR-T cells was complete response in 16 patients (64%). One-year progression-free survival from leukapheresis was 43% with a plateau afterward. One-year relapse-free survival was 79% for patients in complete or partial response at CAR T-cell infusion. The median overall survival was 21.2 months. Twenty-three patients experienced a cytokine release syndrome and 17/25 patients (68%) a neurotoxicity (five grade ≥3). The efficacy endpoints were significantly better in the CAR T-cell group than in the control group (N = 247) (median PFS: 3 months; median OS: 4.7 months; p < 0.001). This series represents the largest cohort of PCNSL treated with CAR T-cells reported worldwide. CAR T-cells are effective in relapsed PCNSL, with a high rate of long-term remission and a reassuring tolerance profile. The results seem clearly superior to those usually observed in this setting.
Language	English
Publishing date	2024-04-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	196767-8
ISSN	1096-8652 ; 0361-8609
ISSN (online)	1096-8652
ISSN	0361-8609
DOI	10.1002/ajh.27316
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Article ; Online: Serum Amyloid A Receptor Blockade and Incorporation into High-Density Lipoprotein Modulates Its Pro-Inflammatory and Pro-Thrombotic Activities on Vascular Endothelial Cells

Belal Chami / Nicola Barrie / Xiaoping Cai / Xiaosuo Wang / Moumita Paul / Rebecca Morton-Chandra / Alexandra Sharland / Joanne M. Dennis / Saul B. Freedman / Paul K. Witting

International Journal of Molecular Sciences, Vol 16, Iss 5, Pp 11101-

2015 Volume 11124

Abstract	The acute phase protein serum amyloid A (SAA), a marker of inflammation, induces expression of pro-inflammatory and pro-thrombotic mediators including ICAM-1, VCAM-1, IL-6, IL-8, MCP-1 and tissue factor (TF) in both monocytes/macrophages and endothelial cells, and induces endothelial dysfunction—a precursor to atherosclerosis. In this study, we determined the effect of pharmacological inhibition of known SAA receptors on pro-inflammatory and pro-thrombotic activities of SAA in human carotid artery endothelial cells (HCtAEC). HCtAEC were pre-treated with inhibitors of formyl peptide receptor-like-1 (FPRL-1), WRW4; receptor for advanced glycation-endproducts (RAGE), (endogenous secretory RAGE; esRAGE) and toll-like receptors-2/4 (TLR2/4) (OxPapC), before stimulation by added SAA. Inhibitor activity was also compared to high-density lipoprotein (HDL), a known inhibitor of SAA-induced effects on endothelial cells. SAA significantly increased gene expression of TF, NFκB and TNF and protein levels of TF and VEGF in HCtAEC. These effects were inhibited to variable extents by WRW4, esRAGE and OxPapC either alone or in combination, suggesting involvement of endothelial cell SAA receptors in pro-atherogenic gene expression. In contrast, HDL consistently showed the greatest inhibitory action, and often abrogated SAA-mediated responses. Increasing HDL levels relative to circulating free SAA may prevent SAA-mediated endothelial dysfunction and ameliorate atherogenesis.
Keywords	serum amyloid A ; inflammation ; atherosclerosis ; high-density lipoprotein ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	616
Language	English
Publishing date	2015-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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