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  1. Article ; Online: Storage cell proliferation during somatic growth establishes that tardigrades are not eutelic organisms.

    Quiroga-Artigas, Gonzalo / Moriel-Carretero, María

    Biology open

    2024  Volume 13, Issue 2

    Abstract: Tardigrades, microscopic ecdysozoans known for extreme environment resilience, were traditionally believed to maintain a constant cell number after completing embryonic development, a phenomenon termed eutely. However, sporadic reports of dividing cells ... ...

    Abstract Tardigrades, microscopic ecdysozoans known for extreme environment resilience, were traditionally believed to maintain a constant cell number after completing embryonic development, a phenomenon termed eutely. However, sporadic reports of dividing cells have raised questions about this assumption. In this study, we explored tardigrade post-embryonic cell proliferation using the model species Hypsibius exemplaris. Comparing hatchlings to adults, we observed an increase in the number of storage cells, responsible for nutrient storage. We monitored cell proliferation via 5-ethynyl-2'-deoxyuridine (EdU) incorporation, revealing large numbers of EdU+ storage cells during growth, which starvation halted. EdU incorporation associated with molting, a vital post-embryonic development process involving cuticle renewal for further growth. Notably, DNA replication inhibition strongly reduced EdU+ cell numbers and caused molting-related fatalities. Our study is the first to demonstrate using molecular approaches that storage cells actively proliferate during tardigrade post-embryonic development, providing a comprehensive insight into replication events throughout their somatic growth. Additionally, our data underscore the significance of proper DNA replication in tardigrade molting and survival. This work definitely establishes that tardigrades are not eutelic, and offers insights into cell cycle regulation, replication stress, and DNA damage management in these remarkable creatures as genetic manipulation techniques emerge within the field.
    MeSH term(s) Adult ; Female ; Humans ; Animals ; Tardigrada ; Cell Proliferation ; DNA Damage ; DNA Replication ; Embryonic Development
    Language English
    Publishing date 2024-02-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2632264-X
    ISSN 2046-6390 ; 2046-6390
    ISSN (online) 2046-6390
    ISSN 2046-6390
    DOI 10.1242/bio.060299
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The Many Faces of Lipids in Genome Stability (and How to Unmask Them).

    Moriel-Carretero, María

    International journal of molecular sciences

    2021  Volume 22, Issue 23

    Abstract: Deep efforts have been devoted to studying the fundamental mechanisms ruling genome integrity preservation. A strong focus relies on our comprehension of nucleic acid and protein interactions. Comparatively, our exploration of whether lipids contribute ... ...

    Abstract Deep efforts have been devoted to studying the fundamental mechanisms ruling genome integrity preservation. A strong focus relies on our comprehension of nucleic acid and protein interactions. Comparatively, our exploration of whether lipids contribute to genome homeostasis and, if they do, how, is severely underdeveloped. This disequilibrium may be understood in historical terms, but also relates to the difficulty of applying classical lipid-related techniques to a territory such as a nucleus. The limited research in this domain translates into scarce and rarely gathered information, which with time further discourages new initiatives. In this review, the ways lipids have been demonstrated to, or very likely do, impact nuclear transactions, in general, and genome homeostasis, in particular, are explored. Moreover, a succinct yet exhaustive battery of available techniques is proposed to tackle the study of this topic while keeping in mind the feasibility and habits of "nucleus-centered" researchers.
    MeSH term(s) Cell Nucleus/metabolism ; Genomic Instability ; Homeostasis ; Humans ; Lipid Metabolism ; Models, Chemical
    Language English
    Publishing date 2021-11-29
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms222312930
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The hypothetical role of phosphatidic acid in subverting ER membranes during SARS-CoV infection.

    Moriel-Carretero, María

    Traffic (Copenhagen, Denmark)

    2020  Volume 21, Issue 8, Page(s) 545–551

    Abstract: Positive sense (+) RNA viruses exploit membranes from a variety of cellular organelles to support the amplification of their genomes. This association concurs with the formation of vesicles whose main morphological feature is that of being wrapped by a ... ...

    Abstract Positive sense (+) RNA viruses exploit membranes from a variety of cellular organelles to support the amplification of their genomes. This association concurs with the formation of vesicles whose main morphological feature is that of being wrapped by a double membrane. In the case of the SARS-CoV virus, the outer membrane is not discrete for each vesicle, but seems to be continuous and shared between many individual vesicles, a difference with other +RNA viruses whose nature has remained elusive. I present morphological, biochemical and pharmacological arguments defending the striking analogy of this arrangement and that of entangled, nascent Lipid Droplets whose birth has been aborted by an excess of Phosphatidic Acid. Since Phosphatidic Acid can be targeted with therapeutical purposes, considering this working hypothesis may prove important in tackling SARS-CoV infection.
    MeSH term(s) Betacoronavirus/pathogenicity ; Betacoronavirus/physiology ; COVID-19 ; Coronavirus Infections/metabolism ; Coronavirus Infections/virology ; Endoplasmic Reticulum/metabolism ; Endoplasmic Reticulum/virology ; Host Microbial Interactions/physiology ; Humans ; Lipid Droplets/metabolism ; Lipid Droplets/virology ; Models, Biological ; Pandemics ; Phosphatidic Acids/metabolism ; Pneumonia, Viral/metabolism ; Pneumonia, Viral/virology ; SARS-CoV-2 ; Virus Replication/physiology
    Chemical Substances Phosphatidic Acids
    Keywords covid19
    Language English
    Publishing date 2020-06-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1483852-7
    ISSN 1600-0854 ; 1398-9219
    ISSN (online) 1600-0854
    ISSN 1398-9219
    DOI 10.1111/tra.12738
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5634: Show issues Location:
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  4. Article ; Online: Auxin alone provokes retention of

    Domeni Zali, Ginola / Moriel-Carretero, María

    microPublication biology

    2023  Volume 2023

    Abstract: The auxin-inducible degradation (AID) system can elicit conditional and reversible protein degradation as a tool to assess the role of essential proteins. Indeed, AID enables functional studies without the possibility of adaptation, which can occur with ... ...

    Abstract The auxin-inducible degradation (AID) system can elicit conditional and reversible protein degradation as a tool to assess the role of essential proteins. Indeed, AID enables functional studies without the possibility of adaptation, which can occur with permanent gene deletions. The AID system relies on the addition of auxin molecules, such as indole-3-acetic acid (IAA), as a means to launch the degradation of the protein of interest. In this context, it is extremely important to control for the effect of auxin addition alone. To study the role of essential proteins in the process of selective mRNA delivery to daughter cells in
    Language English
    Publishing date 2023-02-22
    Publishing country United States
    Document type Journal Article
    ISSN 2578-9430
    ISSN (online) 2578-9430
    DOI 10.17912/micropub.biology.000752
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Daughter cell-targeted mRNAs can achieve segregation without the universal Endoplasmic Reticulum docker She2p.

    Samardak, Kseniya / Moriel-Carretero, María

    microPublication biology

    2021  Volume 2021

    Abstract: The establishment of cell polarity in eukaryotes involves the asymmetric distribution of messenger RNAs (mRNAs). ... ...

    Abstract The establishment of cell polarity in eukaryotes involves the asymmetric distribution of messenger RNAs (mRNAs). In
    Language English
    Publishing date 2021-09-13
    Publishing country United States
    Document type Journal Article
    ISSN 2578-9430
    ISSN (online) 2578-9430
    DOI 10.17912/micropub.biology.000458
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Lack of evidence for condensin or cohesin sequestration on lipid droplets with packing defects.

    Mura, Anaïs / Moriel-Carretero, María

    microPublication biology

    2021  Volume 2021

    Abstract: Lipid droplets (LD) are organelles born from the endoplasmic reticulum that store fats and sterols in an apolar manner both as an energy reservoir and for protective purposes. The LD is delimited by a phospholipid monolayer covered by a rich proteome ... ...

    Abstract Lipid droplets (LD) are organelles born from the endoplasmic reticulum that store fats and sterols in an apolar manner both as an energy reservoir and for protective purposes. The LD is delimited by a phospholipid monolayer covered by a rich proteome that dynamically evolves depending on the nutritional, genetic, pharmacological and environmental cues. Some of these contexts lead to discontinuities in the phospholipid monolayer, termed "packing defects", that expose LD hydrophobic contents to the surrounding water environment. This triggers the unscheduled binding of proteins with affinity for hydrophobic surfaces, a thermodynamically favorable reaction. We have raised in the past the concern that this titration includes proteins with important roles in the nucleus, which entails a risk of genome instability. Analysis of previously published LD proteomes isolated from cells lacking the transcription factor Ino2p, a prototype of LD bearing packing defects, made us concentrate on two subunits of the cohesin (Smc1p and Smc3p) and one of the condensin (Smc2p) complexes, both essential to promote genome integrity by structuring chromosomes. We report that, in disagreement with the proteomic data, we find no evidence of titration of condensin or cohesin subunits onto LD in
    Language English
    Publishing date 2021-11-04
    Publishing country United States
    Document type Journal Article
    ISSN 2578-9430
    ISSN (online) 2578-9430
    DOI 10.17912/micropub.biology.000497
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Microscopy analysis of the smallest subunit of the RPA complex, Rfa3p, prompts consideration of how RPA subunits gather at single-stranded DNA sites.

    Ramonatxo, Agnès / Moriel-Carretero, María

    microPublication biology

    2021  Volume 2021

    Abstract: The heterotrimeric Replication Protein A (RPA) complex preserves genome integrity by protecting the single-stranded DNA that becomes exposed during repair, replication, and recombination. Its two biggest subunits, Rfa1p and Rfa2p (as named ... ...

    Abstract The heterotrimeric Replication Protein A (RPA) complex preserves genome integrity by protecting the single-stranded DNA that becomes exposed during repair, replication, and recombination. Its two biggest subunits, Rfa1p and Rfa2p (as named in
    Language English
    Publishing date 2021-10-27
    Publishing country United States
    Document type Journal Article
    ISSN 2578-9430
    ISSN (online) 2578-9430
    DOI 10.17912/micropub.biology.000493
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The Many Faces of Lipids in Genome Stability (and How to Unmask Them)

    María Moriel-Carretero

    International Journal of Molecular Sciences, Vol 22, Iss 12930, p

    2021  Volume 12930

    Abstract: Deep efforts have been devoted to studying the fundamental mechanisms ruling genome integrity preservation. A strong focus relies on our comprehension of nucleic acid and protein interactions. Comparatively, our exploration of whether lipids contribute ... ...

    Abstract Deep efforts have been devoted to studying the fundamental mechanisms ruling genome integrity preservation. A strong focus relies on our comprehension of nucleic acid and protein interactions. Comparatively, our exploration of whether lipids contribute to genome homeostasis and, if they do, how, is severely underdeveloped. This disequilibrium may be understood in historical terms, but also relates to the difficulty of applying classical lipid-related techniques to a territory such as a nucleus. The limited research in this domain translates into scarce and rarely gathered information, which with time further discourages new initiatives. In this review, the ways lipids have been demonstrated to, or very likely do, impact nuclear transactions, in general, and genome homeostasis, in particular, are explored. Moreover, a succinct yet exhaustive battery of available techniques is proposed to tackle the study of this topic while keeping in mind the feasibility and habits of “nucleus-centered” researchers.
    Keywords nuclear homeostasis ; lipids ; genome stability ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2021-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Nuclear envelope-remodeling events as models to assess the potential role of membranes on genome stability.

    Bâcle, Janélie / Groizard, Léa / Kumanski, Sylvain / Moriel-Carretero, María

    FEBS letters

    2023  Volume 597, Issue 15, Page(s) 1946–1956

    Abstract: The nuclear envelope (NE) encloses the genetic material and functions in chromatin organization and stability. In Saccharomyces cerevisiae, the NE is bound to the ribosomal DNA (rDNA), highly repeated and transcribed, thus prone to genetic instability. ... ...

    Abstract The nuclear envelope (NE) encloses the genetic material and functions in chromatin organization and stability. In Saccharomyces cerevisiae, the NE is bound to the ribosomal DNA (rDNA), highly repeated and transcribed, thus prone to genetic instability. While tethering limits instability, it simultaneously triggers notable NE remodeling. We posit here that NE remodeling may contribute to genome integrity maintenance. The NE importance in genome expression, structure, and integrity is well recognized, yet studies mostly focus on peripheral proteins and nuclear pores, not on the membrane itself. We recently characterized a NE invagination drastically obliterating the rDNA, which we propose here as a model to probe if and how membranes play an active role in genome stability preservation.
    MeSH term(s) Humans ; Nuclear Envelope/genetics ; Nuclear Pore/genetics ; Nuclear Pore/metabolism ; Genomic Instability ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; DNA, Ribosomal/genetics ; DNA, Ribosomal/metabolism
    Chemical Substances DNA, Ribosomal
    Language English
    Publishing date 2023-06-26
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1002/1873-3468.14688
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 282: Show issues Location:
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  10. Article ; Online: An Expansion of the Endoplasmic Reticulum that Halts Autophagy is Permissive to Genome Instability.

    Lara-Barba, Eliana / Torán-Vilarrubias, Alba / Moriel-Carretero, María

    Contact (Thousand Oaks (Ventura County, Calif.))

    2023  Volume 6, Page(s) 25152564231157706

    Abstract: The links between autophagy and genome stability, and whether they are important for lifespan and health, are not fully understood. We undertook a study to explore this notion at the molecular level ... ...

    Abstract The links between autophagy and genome stability, and whether they are important for lifespan and health, are not fully understood. We undertook a study to explore this notion at the molecular level using
    Language English
    Publishing date 2023-02-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2964312-0
    ISSN 2515-2564 ; 2515-2564
    ISSN (online) 2515-2564
    ISSN 2515-2564
    DOI 10.1177/25152564231157706
    Database MEDical Literature Analysis and Retrieval System OnLINE

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