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  1. Article: Cytotoxicity and Genotoxicity of Nanomaterials.

    Valdiglesias, Vanessa

    Nanomaterials (Basel, Switzerland)

    2022  Volume 12, Issue 4

    Abstract: Nanomaterials (NMs) are of significant relevance due to their unique physicochemical properties, which have been extensively exploited for widespread applications in human healthcare and consumer goods, such as cosmetics and textiles [ ... ]. ...

    Abstract Nanomaterials (NMs) are of significant relevance due to their unique physicochemical properties, which have been extensively exploited for widespread applications in human healthcare and consumer goods, such as cosmetics and textiles [...].
    Language English
    Publishing date 2022-02-14
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2662255-5
    ISSN 2079-4991
    ISSN 2079-4991
    DOI 10.3390/nano12040634
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cytotoxicity and Genotoxicity of Nanomaterials

    Vanessa Valdiglesias

    Nanomaterials, Vol 12, Iss 634, p

    2022  Volume 634

    Abstract: Nanomaterials (NMs) are of significant relevance due to their unique physicochemical properties, which have been extensively exploited for widespread applications in human healthcare and consumer goods, such as cosmetics and textiles [.] ...

    Abstract Nanomaterials (NMs) are of significant relevance due to their unique physicochemical properties, which have been extensively exploited for widespread applications in human healthcare and consumer goods, such as cosmetics and textiles [.]
    Keywords n/a ; Chemistry ; QD1-999
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: The impact of nanotechnology in the current universal COVID-19 crisis. Let's not forget nanosafety!

    Valdiglesias, Vanessa / Laffon, Blanca

    Nanotoxicology

    2020  Volume 14, Issue 8, Page(s) 1013–1016

    Keywords covid19
    Language English
    Publishing date 2020-06-18
    Publishing country England
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 2237988-5
    ISSN 1743-5404 ; 1743-5390
    ISSN (online) 1743-5404
    ISSN 1743-5390
    DOI 10.1080/17435390.2020.1780332
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: The impact of nanotechnology in the current universal COVID-19 crisis. Let’s not forget nanosafety!

    Valdiglesias, Vanessa / Laffon, Blanca

    Nanotoxicology

    2020  Volume 14, Issue 8, Page(s) 1013–1016

    Keywords Toxicology ; Biomedical Engineering ; covid19
    Language English
    Publisher Informa UK Limited
    Publishing country uk
    Document type Article ; Online
    ZDB-ID 2237988-5
    ISSN 1743-5404 ; 1743-5390
    ISSN (online) 1743-5404
    ISSN 1743-5390
    DOI 10.1080/17435390.2020.1780332
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Multiparametric

    Brandão, Fátima / Costa, Carla / Bessa, Maria João / Valdiglesias, Vanessa / Hellack, Bryan / Haase, Andrea / Fraga, Sónia / Teixeira, João Paulo

    Nanotoxicology

    2023  Volume 17, Issue 6-7, Page(s) 511–528

    Abstract: The hazard posed to human health by inhaled amorphous silica nanomaterials ( ... ...

    Abstract The hazard posed to human health by inhaled amorphous silica nanomaterials (aSiO
    MeSH term(s) Rats ; Humans ; Animals ; Alveolar Epithelial Cells ; Silicon Dioxide/toxicity ; DNA Damage ; Comet Assay ; Nanostructures/toxicity
    Chemical Substances Silicon Dioxide (7631-86-9)
    Language English
    Publishing date 2023-12-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2237988-5
    ISSN 1743-5404 ; 1743-5390
    ISSN (online) 1743-5404
    ISSN 1743-5390
    DOI 10.1080/17435390.2023.2265481
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Assessing the

    Bessa, Maria João / Brandão, Fátima / Rosário, Fernanda / Moreira, Luciana / Reis, Ana Teresa / Valdiglesias, Vanessa / Laffon, Blanca / Fraga, Sónia / Teixeira, João Paulo

    Journal of toxicology and environmental health. Part B, Critical reviews

    2023  Volume 26, Issue 2, Page(s) 67–96

    Abstract: Several studies have been conducted to address the potential adverse health risks attributed to exposure to nanoscale materials. ... ...

    Abstract Several studies have been conducted to address the potential adverse health risks attributed to exposure to nanoscale materials. While
    MeSH term(s) Humans ; Respiratory System ; Dust ; Inhalation Exposure/adverse effects ; Nanoparticles/toxicity
    Chemical Substances Dust
    Language English
    Publishing date 2023-01-24
    Publishing country England
    Document type Review ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1415246-0
    ISSN 1521-6950 ; 1093-7404
    ISSN (online) 1521-6950
    ISSN 1093-7404
    DOI 10.1080/10937404.2023.2166638
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4944: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Genomic instability as a main driving factor of unsuccessful ageing: Potential for translating the use of micronuclei into clinical practice.

    Laffon, Blanca / Bonassi, Stefano / Costa, Solange / Valdiglesias, Vanessa

    Mutation research. Reviews in mutation research

    2020  Volume 787, Page(s) 108359

    Abstract: Genome instability denotes an increased tendency to alterations in the genome during cell life cycle, driven by a large variety of endogenous and exogenous insults. Ageing is characterized by the presence of damage to various cellular constituents, but ... ...

    Abstract Genome instability denotes an increased tendency to alterations in the genome during cell life cycle, driven by a large variety of endogenous and exogenous insults. Ageing is characterized by the presence of damage to various cellular constituents, but genome alterations, randomly accumulating with age in different tissues, constitute the key target in this process, and are believed to be the main factor of ageing. Age-related failure of DNA repair pathways allows DNA lesions to occur more frequently, and their accumulation over time contributes to the age-associated decrease in genome integrity in somatic cells. The micronucleus (MN) test is one of the most widely used assays to evaluate genomic instability in different surrogate tissues. A large number of studies has consistently shown a progressive increase in MN frequency with age, starting from very young age groups onwards. Therefore, MN frequency is a suitable biomarker of genomic instability in ageing. Frailty is a multidimensional geriatric syndrome of unsuccessful ageing, characterized by decreased biological reserves and increased vulnerability to external stressors, involving a higher risk of negative health outcomes. Although there is a well-founded belief that genome instability is involved in the frailty syndrome, only two studies investigated the relationship between MN frequency and frailty, not allowing to draw a definite conclusion on the utility of this biomarker for frailty detection. The use of MN and other genomic biomarkers in the detection and follow-up of patients affected by or at risk of frailty has the potential to accumulate evidence on the clinical impact of this approach in the identification and control of frailty in older people.
    MeSH term(s) Aged ; Aged, 80 and over ; Aging/genetics ; Aging/physiology ; DNA Repair/genetics ; DNA Repair/physiology ; Female ; Frail Elderly ; Genomic Instability/genetics ; Humans ; Male ; Micronucleus Tests/methods
    Language English
    Publishing date 2020-11-27
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2727833-5
    ISSN 1388-2139 ; 1383-5742
    ISSN (online) 1388-2139
    ISSN 1383-5742
    DOI 10.1016/j.mrrev.2020.108359
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1316 -Rev-: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Applicability of flow cytometry γH2AX assay in population studies: suitability of fresh and frozen whole blood samples.

    Laffon, Blanca / Sánchez-Flores, María / Fernández-Bertólez, Natalia / Pásaro, Eduardo / Valdiglesias, Vanessa

    Archives of toxicology

    2021  Volume 95, Issue 5, Page(s) 1843–1851

    Abstract: Phosphorylation of H2AX histone (γH2AX) represents an early event in the DNA damage response against double-strand breaks (DSB); hence, its measurement provides a surrogate biomarker of DSB. Recently, we reported initial steps in the standardization of ... ...

    Abstract Phosphorylation of H2AX histone (γH2AX) represents an early event in the DNA damage response against double-strand breaks (DSB); hence, its measurement provides a surrogate biomarker of DSB. Recently, we reported initial steps in the standardization of γH2AX assay in peripheral blood leukocytes (PBL), addressing the possibility of using cryopreserved samples, and the need of phytohaemagglutinin (PHA) stimulation prior analysis (Toxicol Sci 2015, 144:406-13). Validating the use of whole blood samples as cell specimen for this assay would be particularly useful for human population studies. Hence, in the current study we determined for the first time the feasibility of whole blood samples, both fresh and frozen, to be used in the γH2AX assay, evaluated by flow cytometry, and the convenience of PHA stimulation. Freshly collected and cryopreserved whole blood samples were treated with bleomycin (BLM), actinomycin-D (Act-D) and mitomycin C (MMC); half of the samples were previously incubated with PHA. Results were compared with those from PBL. Negative responses in MMC treatments were probably due to the quiescence of unstimulated cells, or to the short treatment time in PHA stimulated cells. Fresh whole blood samples exhibited a more intense response to BLM and Act-D treatments in stimulated cells, probably due to DSB indirectly produced from other less relevant types of DNA damage. Results obtained in frozen whole blood samples indicate that PHA stimulation is not advisable. In conclusion, this study demonstrates that whole blood samples can be used to assess DSB-related genotoxicity by the flow cytometry γH2AX assay.
    MeSH term(s) Biological Assay/methods ; Biomarkers/metabolism ; DNA Breaks, Double-Stranded ; DNA Damage ; Flow Cytometry ; Histones/blood ; Histones/metabolism ; Humans ; Mutagens ; Phosphorylation
    Chemical Substances Biomarkers ; H2AX protein, human ; Histones ; Mutagens
    Language English
    Publishing date 2021-02-23
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 124992-7
    ISSN 1432-0738 ; 0340-5761
    ISSN (online) 1432-0738
    ISSN 0340-5761
    DOI 10.1007/s00204-021-03009-z
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    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.90: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Salivary leucocytes as suitable biomatrix for the comet assay in human biomonitoring studies.

    Fernández-Bertólez, Natalia / Azqueta, Amaya / Pásaro, Eduardo / Laffon, Blanca / Valdiglesias, Vanessa

    Archives of toxicology

    2021  Volume 95, Issue 6, Page(s) 2179–2187

    Abstract: Peripheral blood leucocytes (PBL) have been traditionally used to investigate DNA damage by the comet assay in population studies, but validating alternative non-invasive samples would expand the application of this assay in human biomonitoring. The ... ...

    Abstract Peripheral blood leucocytes (PBL) have been traditionally used to investigate DNA damage by the comet assay in population studies, but validating alternative non-invasive samples would expand the application of this assay in human biomonitoring. The objectives of this study were (i) to test the validity of salivary leucocytes as a proper biomatrix for the comet assay, (ii) to evaluate the ability of this approach to detect different types of primary and oxidative DNA damage, and (iii) to determine whether frozen salivary leucocytes are still suitable for displaying those types of DNA damage. Fresh and frozen leucocytes isolated from saliva samples (six healthy non-smoking volunteers), were exposed to four genotoxic agents inducing different types of DNA damage, both primary (methyl methanesulfonate, actinomycin-D, ultraviolet radiation) and oxidative (potassium bromate), and standard or enzyme-modified comet assay was conducted. Results were compared with those obtained from PBL. Cells exposed to the four genotoxic agents showed dose-dependent increases of primary and oxidative DNA damage, demonstrating the suitability of all these samples to detect genetic damage from different origin. When comparing baseline levels of DNA damage, just a slight significant increase in primary DNA damage was observed in frozen salivary leucocytes regarding the other biomatrices, but similar results were obtained regarding sensitivity to DNA damage induction by all agents tested. This study demonstrates that salivary leucocytes can be employed in comet assay as an alternative or complement to blood samples. Frozen salivary leucocytes were proved to be a very convenient sample in large biomonitoring studies.
    MeSH term(s) Adult ; Biological Monitoring/methods ; Comet Assay/methods ; DNA Damage/drug effects ; DNA Damage/radiation effects ; Female ; Freezing ; Humans ; Leukocytes/cytology ; Leukocytes/drug effects ; Leukocytes/radiation effects ; Male ; Middle Aged ; Saliva/cytology
    Language English
    Publishing date 2021-03-31
    Publishing country Germany
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Validation Study
    ZDB-ID 124992-7
    ISSN 1432-0738 ; 0340-5761
    ISSN (online) 1432-0738
    ISSN 0340-5761
    DOI 10.1007/s00204-021-03038-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.90: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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