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  1. Article: Multigene Phylogeny and Pathogenicity Trials Revealed

    Achilonu, Conrad Chibunna / Marais, Gert Johannes / Ghosh, Soumya / Gryzenhout, Marieka

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 5

    Abstract: The pecan ( ...

    Abstract The pecan (
    Language English
    Publishing date 2023-05-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12050672
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Microbe Mail: A microbiology and infectious diseases podcast for clinicians and students.

    Chibabhai, Vindana / Marais, Gert J K / Alex, Vinitha

    Southern African journal of infectious diseases

    2023  Volume 38, Issue 1, Page(s) 570

    Abstract: Microbe Mail medical podcast is an audio-only informal educational platform, which addresses widespread topics in medical microbiology and infectious diseases (ID), with a particular focus on low- and middle-income settings. Podcasting has become a ... ...

    Abstract Microbe Mail medical podcast is an audio-only informal educational platform, which addresses widespread topics in medical microbiology and infectious diseases (ID), with a particular focus on low- and middle-income settings. Podcasting has become a common form of informal learning in healthcare education and for continual professional development (CPD).
    Contribution: In this article, we discuss the development of the Microbe Mail podcast, its informal microbiology and ID education impact in the 2 years since commencement and future directions to improve uptake in Africa and low- and middle-income countries.
    Language English
    Publishing date 2023-12-21
    Publishing country South Africa
    Document type Journal Article
    ZDB-ID 3046282-4
    ISSN 2313-1810 ; 2312-0053
    ISSN (online) 2313-1810
    ISSN 2312-0053
    DOI 10.4102/sajid.v38i1.570
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Differential Detection of

    Achilonu, Conrad Chibunna / Gryzenhout, Marieka / Marais, Gert Johannes / Ghosh, Soumya

    Genes

    2023  Volume 14, Issue 5

    Abstract: Alternaria black spot disease on pecan is caused by the opportunistic ... ...

    Abstract Alternaria black spot disease on pecan is caused by the opportunistic pathogen
    MeSH term(s) Alternaria/genetics ; Carya/genetics ; Polymorphism, Restriction Fragment Length ; Haplotypes ; Polymerase Chain Reaction
    Language English
    Publishing date 2023-05-20
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14051115
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: In Vitro Evaluation of Azoxystrobin, Boscalid, Fentin-Hydroxide, Propiconazole, Pyraclostrobin Fungicides against

    Achilonu, Conrad Chibunna / Gryzenhout, Marieka / Ghosh, Soumya / Marais, Gert Johannes

    Microorganisms

    2023  Volume 11, Issue 7

    Abstract: Black spot disease or Alternaria black spot (ABS) of pecan ( ...

    Abstract Black spot disease or Alternaria black spot (ABS) of pecan (
    Language English
    Publishing date 2023-06-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11071691
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Random amplified microsatellites (RAMS) analysis ascertains genetic variation of

    Achilonu, Conrad Chibunna / Gryzenhout, Marieka / Marais, Gert Johannes / Madisha, M Thabang / Ghosh, Soumya

    Frontiers in genetics

    2023  Volume 14, Page(s) 1213102

    Abstract: Limited information regarding the occurrence of black spot disease of pecan ( ...

    Abstract Limited information regarding the occurrence of black spot disease of pecan (
    Language English
    Publishing date 2023-09-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2023.1213102
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: A case for investment in clinical metagenomics in low-income and middle-income countries.

    Marais, Gert / Hardie, Diana / Brink, Adrian

    The Lancet. Microbe

    2022  Volume 4, Issue 3, Page(s) e192–e199

    Abstract: Clinical metagenomics is the diagnostic approach with the broadest capacity to detect both known and novel pathogens. Clinical metagenomics is costly to run and requires infrastructure, but the use of next-generation sequencing for SARS-CoV-2 molecular ... ...

    Abstract Clinical metagenomics is the diagnostic approach with the broadest capacity to detect both known and novel pathogens. Clinical metagenomics is costly to run and requires infrastructure, but the use of next-generation sequencing for SARS-CoV-2 molecular epidemiology in low-income and middle-income countries (LMICs) offers an opportunity to direct this infrastructure to the establishment of clinical metagenomics programmes. Local implementation of clinical metagenomics is important to create relevant systems and evaluate cost-effective methodologies for its use, as well as to ensure that reference databases and result interpretation tools are appropriate to local epidemiology. Rational implementation, based on the needs of LMICs and the available resources, could ultimately improve individual patient care in instances in which available diagnostics are inadequate and supplement emerging infectious disease surveillance systems to ensure the next pandemic pathogen is quickly identified.
    MeSH term(s) Humans ; SARS-CoV-2 ; COVID-19 ; Developing Countries ; Metagenomics ; Investments
    Language English
    Publishing date 2022-12-20
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ISSN 2666-5247
    ISSN (online) 2666-5247
    DOI 10.1016/S2666-5247(22)00328-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Clinical metagenomics in a resource-limited setting.

    Marais, Gert / Valley-Omar, Ziyaad / Marais, Suzaan / McMullen, Kate / Bateman, Kathleen / der Westhuizen, Diederick van / Maseko, Moepeng / Hardie, Diana / Brink, Adrian

    The Journal of infection

    2023  Volume 87, Issue 6, Page(s) 604–606

    MeSH term(s) Humans ; Resource-Limited Settings ; Metagenomics
    Language English
    Publishing date 2023-10-17
    Publishing country England
    Document type Letter
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2023.10.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1501: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 48: Show issues

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  8. Article ; Online: Management of hyperlipidaemia.

    Marais, A D / Blom, D J / Raal, F J

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde

    2024  Volume 114, Issue 3, Page(s) e1016

    MeSH term(s) Humans ; Hyperlipidemias/drug therapy ; South Africa ; Risk Factors
    Language English
    Publishing date 2024-03-18
    Publishing country South Africa
    Document type Journal Article
    ZDB-ID 390968-2
    ISSN 2078-5135 ; 0038-2469 ; 0256-9574
    ISSN (online) 2078-5135
    ISSN 0038-2469 ; 0256-9574
    DOI 10.7196/SAMJ.2024.v114i3.1964
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.406: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 645: Show issues

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  9. Article ; Online: Carbapenem-resistant

    Marais, Gert / Moodley, Clinton / Claassen-Weitz, Shantelle / Patel, Fadheela / Prentice, Elizabeth / Tootla, Hafsah / Nyakutira, Nyasha / Lennard, Katie / Reddy, Kessendri / Bamford, Colleen / Niehaus, Abraham / Whitelaw, Andrew / Brink, Adrian

    JAC-antimicrobial resistance

    2024  Volume 6, Issue 2, Page(s) dlae050

    Abstract: Background: The molecular epidemiology of carbapenem-resistant Enterobacterales in Cape Town remains largely unknown.: Objectives: This study aimed to describe the molecular epidemiology, resistome, virulome and mobilome of carbapenem-resistant : ... ...

    Abstract Background: The molecular epidemiology of carbapenem-resistant Enterobacterales in Cape Town remains largely unknown.
    Objectives: This study aimed to describe the molecular epidemiology, resistome, virulome and mobilome of carbapenem-resistant
    Methods: Eighty-five CRKP isolates from hospitalized patients underwent WGS as part of a prospective, multicentre, cross-sectional study, conducted between 1 November 2020 and 30 November 2022, across public-sector and private-sector hospitals in Cape Town, South Africa.
    Results: MLST revealed three novel types, ST6785, ST6786 and ST6787, while the most common were ST219, ST307, ST17, ST13 and ST2497. Different predominant clones were noted in each hospital. The most common carbapenemase gene was
    Conclusions: CRKP epidemiology in Cape Town reflects institutionally dominant, rather than regional, clones. The most prevalent carbapenemase gene was
    Language English
    Publishing date 2024-03-25
    Publishing country England
    Document type Journal Article ; Comment
    ISSN 2632-1823
    ISSN (online) 2632-1823
    DOI 10.1093/jacamr/dlae050
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Differential Detection of Alternaria alternata Haplotypes Isolated from Carya illinoinensis Using PCR-RFLP Analysis of Alt a1 Gene Region

    Achilonu, Conrad Chibunna / Gryzenhout, Marieka / Marais, Gert Johannes / Ghosh, Soumya

    Genes (Basel). 2023 May 20, v. 14, no. 5

    2023  

    Abstract: Alternaria black spot disease on pecan is caused by the opportunistic pathogen Alternaria alternata and poses a serious threat to the local South African and global pecan industry. Several diagnostic molecular marker applications have been established ... ...

    Abstract Alternaria black spot disease on pecan is caused by the opportunistic pathogen Alternaria alternata and poses a serious threat to the local South African and global pecan industry. Several diagnostic molecular marker applications have been established and used in the screening of various fungal diseases worldwide. The present study investigated the potential for polymorphism within samples of A. alternata isolates obtained from eight different geographical locations in South Africa. Pecan (Carya illinoinensis) leaves, shoots, and nuts-in-shuck with Alternaria black spot disease were sampled, and 222 A. alternata isolates were retrieved. For rapid screening to identify Alternaria black spot pathogens, polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis of the Alternaria major allergen (Alt a1) gene region was used, followed by the digestion of the amplicons with HaeIII and HinfI endonucleases. The assay resulted in five (HaeIII) and two (HinfI) band patterns. Unique banding patterns from the two endonucleases showed the best profile and isolates were grouped into six clusters using a UPGMA (unweighted pair group method with arithmetic averages) distance matrix (Euclidean) dendrogram method on R-Studio. The analysis confirmed that the genetic diversity of A. alternata does not depend on host tissues or the pecan cultivation region. The grouping of selected isolates was confirmed by DNA sequence analysis. The Alt a1 phylogeny corroborated no speciation within the dendrogram groups and showed 98–100% bootstrap similarity. This study reports the first documented rapid and reliable technique for routine screening identification of pathogens causing Alternaria black spot in South Africa.
    Keywords Alternaria alternata ; Carya illinoinensis ; allergens ; arithmetics ; digestion ; fungi ; genes ; genetic markers ; genetic variation ; haplotypes ; industry ; nucleotide sequences ; opportunistic pathogens ; pecans ; phylogeny ; polymerase chain reaction ; sequence analysis ; South Africa
    Language English
    Dates of publication 2023-0520
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14051115
    Database NAL-Catalogue (AGRICOLA)

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