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  1. Article ; Online: Interactions between SARS-CoV-2 N-protein and α-synuclein accelerate amyloid formation

    Semerdzhiev, Slav / Fakhree, Mohammad Amin Abolghassemi / Segers-Nolten, Ine / Blum, Christian / Claessens, Mireille M.A.E.

    bioRxiv

    Abstract: First cases that point at a correlation between SARS-CoV-2 infections and the development of Parkinson9s disease have been reported. Currently it is unclear if there also is a direct causal link between these diseases. To obtain first insights into a ... ...

    Abstract First cases that point at a correlation between SARS-CoV-2 infections and the development of Parkinson9s disease have been reported. Currently it is unclear if there also is a direct causal link between these diseases. To obtain first insights into a possible molecular relation between viral infections and the aggregation of α-synuclein protein into amyloid fibrils characteristic for Parkinson9s disease, we investigated the effect of the presence of SARS-CoV-2 proteins on α synuclein aggregation. We show, in test tube experiments, that SARS-CoV-2 S-protein has no effect on α-synuclein aggregation while SARS-CoV-2 N-protein considerably speeds up the aggregation process. We observe the formation of multi-protein complexes, and eventually amyloid fibrils. Microinjection of N-protein in SHSY-5Y cells disturbed the α-synuclein proteostasis and increased cell death. Our results point toward direct interactions between the N-protein of SARS-CoV-2 and α-synuclein as molecular basis for the observed coincidence between SARS-CoV-2 infections and Parkinsonism.
    Keywords covid19
    Language English
    Publishing date 2021-04-12
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2021.04.12.439549
    Database COVID19

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  2. Article: Ejaculation as a treatment for nasal congestion in men is inconvenient, unreliable and potentially hazardous.

    Fakhree, Mohammad Amin Abolghassemi

    Medical hypotheses

    2008  Volume 71, Issue 5, Page(s) 809

    MeSH term(s) Ejaculation ; Humans ; Male ; Models, Theoretical ; Nasal Obstruction/therapy ; Respiration Disorders/therapy ; Sympathetic Nervous System
    Language English
    Publishing date 2008-11
    Publishing country United States
    Document type Comment ; Letter
    ZDB-ID 193145-3
    ISSN 1532-2777 ; 0306-9877
    ISSN (online) 1532-2777
    ISSN 0306-9877
    DOI 10.1016/j.mehy.2008.07.022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1132: Show issues Location:
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  3. Article: Earwax (cerumen) might be formulated into a safe and biodegradable insect repellent.

    Ahmadian, Somaieh / Fakhree, Mohammad Amin Abolghassemi

    Medical hypotheses

    2009  Volume 72, Issue 3, Page(s) 370–371

    MeSH term(s) Biodegradation, Environmental ; Cerumen/chemistry ; Drug Compounding/methods ; Insect Repellents/chemistry
    Chemical Substances Insect Repellents
    Language English
    Publishing date 2009-03
    Publishing country United States
    Document type Letter
    ZDB-ID 193145-3
    ISSN 1532-2777 ; 0306-9877
    ISSN (online) 1532-2777
    ISSN 0306-9877
    DOI 10.1016/j.mehy.2008.09.036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article ; Online: Henna (Lawsonia inermis) might be used to prevent mycotic infection.

    Ahmadian, Somaieh / Fakhree, Mohammad Amin Abolghassemi

    Medical hypotheses

    2009  Volume 73, Issue 4, Page(s) 629–630

    MeSH term(s) Antifungal Agents/therapeutic use ; Humans ; Lawsonia Plant/chemistry ; Mycoses/drug therapy ; Phytotherapy/methods ; Plant Extracts/therapeutic use
    Chemical Substances Antifungal Agents ; Plant Extracts
    Language English
    Publishing date 2009-10
    Publishing country United States
    Document type Letter
    ZDB-ID 193145-3
    ISSN 1532-2777 ; 0306-9877
    ISSN (online) 1532-2777
    ISSN 0306-9877
    DOI 10.1016/j.mehy.2009.06.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1132: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article: Incense as a potential drug-delivery method.

    Fakhree, Mohammad Amin Abolghassemi / Jouyban, Abolghasem

    Medical hypotheses

    2008  Volume 71, Issue 6, Page(s) 979

    MeSH term(s) Dosage Forms ; Drug Delivery Systems/methods ; Humans ; Odorants
    Chemical Substances Dosage Forms
    Language English
    Publishing date 2008-12
    Publishing country United States
    Document type Letter
    ZDB-ID 193145-3
    ISSN 1532-2777 ; 0306-9877
    ISSN (online) 1532-2777
    ISSN 0306-9877
    DOI 10.1016/j.mehy.2008.07.025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1132: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: The importance of dielectric constant for drug solubility prediction in binary solvent mixtures: electrolytes and zwitterions in water + ethanol.

    Abolghassemi Fakhree, Mohammad Amin / Delgado, Daniel R / Martínez, Fleming / Jouyban, Abolghasem

    AAPS PharmSciTech

    2010  Volume 11, Issue 4, Page(s) 1726–1729

    MeSH term(s) Drug Compounding ; Drug Discovery ; Electrolytes ; Ethanol ; Forecasting ; Models, Chemical ; Pharmaceutical Preparations/chemistry ; Solubility ; Solvents ; Water
    Chemical Substances Electrolytes ; Pharmaceutical Preparations ; Solvents ; Water (059QF0KO0R) ; Ethanol (3K9958V90M)
    Language English
    Publishing date 2010-12-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2052070-0
    ISSN 1530-9932 ; 1530-9932
    ISSN (online) 1530-9932
    ISSN 1530-9932
    DOI 10.1208/s12249-010-9552-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Cellular toxicity of nanogenomedicine in MCF-7 cell line: MTT assay.

    Ahmadian, Somaieh / Barar, Jaleh / Saei, Amir Ata / Fakhree, Mohammad Amin Abolghassemi / Omidi, Yadollah

    Journal of visualized experiments : JoVE

    2009  , Issue 26

    Abstract: Cytotoxicity of the futuristic nanogenomedicine (e.g., short interfering RNA and antisense) may hamper its clinical development. Of these, the gene-based medicine and/or its carrier may elicit cellular toxicity. For assessment of such cytotoxicity, a ... ...

    Abstract Cytotoxicity of the futuristic nanogenomedicine (e.g., short interfering RNA and antisense) may hamper its clinical development. Of these, the gene-based medicine and/or its carrier may elicit cellular toxicity. For assessment of such cytotoxicity, a common methodology is largely dependent upon utilization of the 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay which has been widely used as a colorimetric approach based on the activity of mitochondrial dehydrogenase enzymes in cells. In this current investigation, MCF-7 cells were inoculated in 96-well plate and at 50% confluency they were treated with different nanopolyplexes and subjected to MTT assay after 24 hours. Water soluble yellow MTT is metabolized by the metabolically active cells to the water insoluble purple formazan, which is further dissolved in dimethylsulfoxide and Sornson s buffer pH 10.5. The resultant product can be quantified by spectrophotometry using a plate reader at 570 nm.
    MeSH term(s) Breast Neoplasms ; Cell Line, Tumor ; Cell Survival/drug effects ; Coloring Agents/chemistry ; Coloring Agents/metabolism ; Humans ; Nanostructures/toxicity ; Spectrophotometry/methods ; Tetrazolium Salts/chemistry ; Tetrazolium Salts/metabolism ; Thiazoles/chemistry ; Thiazoles/metabolism ; Toxicity Tests/methods
    Chemical Substances Coloring Agents ; Tetrazolium Salts ; Thiazoles ; thiazolyl blue (EUY85H477I)
    Language English
    Publishing date 2009-04-03
    Publishing country United States
    Document type Journal Article ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/1191
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Cellular toxicity of nanogenomedicine in mcf-7 cell line: mtt assay

    Ahmadian, Somaieh / Barar, Jaleh / Saei, Amir Ata / Fakhree, Mohammad Amin Abolghassemi / Omidi, Yadollah

    Journal of visualized experiments. 2009 Apr. 03, , no. 26

    2009  

    Abstract: Cytotoxicity of the futuristic nanogenomedicine (e.g., short interfering RNA and antisense) may hamper its clinical development. Of these, the gene-based medicine and/or its carrier may elicit cellular toxicity. For assessment of such cytotoxicity, a ... ...

    Abstract Cytotoxicity of the futuristic nanogenomedicine (e.g., short interfering RNA and antisense) may hamper its clinical development. Of these, the gene-based medicine and/or its carrier may elicit cellular toxicity. For assessment of such cytotoxicity, a common methodology is largely dependent upon utilization of the 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay which has been widely used as a colorimetric approach based on the activity of mitochondrial dehydrogenase enzymes in cells. In this current investigation, MCF-7 cells were inoculated in 96-well plate and at 50% confluency they were treated with different nanopolyplexes and subjected to MTT assay after 24 hours. Water soluble yellow MTT is metabolized by the metabolically active cells to the water insoluble purple formazan, which is further dissolved in dimethylsulfoxide and Sornson s buffer pH 10.5. The resultant product can be quantified by spectrophotometry using a plate reader at 570 nm.
    Keywords buffer index ; buffers ; cell lines ; colorimetry ; cytotoxicity ; dimethyl sulfoxide ; enzymes ; medicine ; mitochondria ; pH ; small interfering RNA ; spectroscopy ; toxicity testing ; water solubility
    Language English
    Dates of publication 2009-0403
    Size p. e1191.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/1191
    Database NAL-Catalogue (AGRICOLA)

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