Article ; Online: Highly efficient site-specific integration of DNA fragments into the honeybee genome using CRISPR/Cas9.
2022 Volume 12, Issue 6
Abstract: Functional genetic studies in honeybees have been limited to transposon mediated transformation and site directed mutagenesis tools. However, site- and sequence-specific manipulations that insert DNA fragments or replace sequences at specific target ... ...
| Abstract | Functional genetic studies in honeybees have been limited to transposon mediated transformation and site directed mutagenesis tools. However, site- and sequence-specific manipulations that insert DNA fragments or replace sequences at specific target sites are lacking. Such tools would enable the tagging of proteins, the expression of reporters and site-specific amino acid changes, which are all gold standard manipulations for physiological, organismal, and genetic studies. However, such manipulations must be very efficient in honeybees since screening and crossing procedures are laborious due to their social organization. Here, we report an accurate and remarkably efficient site-specific integration of DNA-sequences into the honeybee genome using clustered regularly interspaced short palindromic repeat/clustered regularly interspaced short palindromic repeat-associated protein 9-mediated homology-directed repair. We employed early embryonic injections and selected a highly efficient sgRNA in order to insert 294 and 729 bp long DNA sequences into a specific locus at the dsx gene. These sequences were locus-specifically integrated in 57% and 59% of injected bees. Most importantly, 21% and 25% of the individuals lacked the wildtype sequence demonstrating that we generated homozygous mutants in which all cells are affected (no mosaicism). The highly efficient, locus-specific insertions of nucleotide sequences generating homozygous mutants demonstrate that systematic molecular studies for honeybees are in hand that allow somatic mutation approaches via workers or studies in the next generation using queens with their worker progeny. The employment of early embryonic injections and screenings of highly efficient sgRNAs may offer the prospect of highly successful sequence- and locus-specific mutations also in other organisms. |
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| MeSH term(s) | Animals ; Base Sequence ; Bees/genetics ; CRISPR-Cas Systems ; DNA ; Gene Editing/methods ; Genome ; Mutation |
| Chemical Substances | DNA (9007-49-2) |
| Language | English |
| Publishing date | 2022-05-06 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2629978-1 |
| ISSN | 2160-1836 ; 2160-1836 |
| ISSN (online) | 2160-1836 |
| ISSN | 2160-1836 |
| DOI | 10.1093/g3journal/jkac098 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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