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  1. Article: Problems with Beta Adrenergic Blocking Drugs and other Antihypertensive Drugs.

    Prichard, B N

    Proceedings of the Royal Society of Medicine

    2010  Volume 70, Issue Suppl 5, Page(s) 40–44

    Language English
    Publishing date 2010-10-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 209243-8
    ISSN 0035-9157
    ISSN 0035-9157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A standardized approach to the evaluation of antivirals against DNA viruses: Polyomaviruses and lymphotropic herpesviruses.

    Keith, Kathy A / Hartline, Caroll B / Bowlin, Terry L / Prichard, Mark N

    Antiviral research

    2018  Volume 159, Page(s) 122–129

    Abstract: The search for new compounds with a broad spectrum of antiviral activity is important and requires the evaluation of many compounds against several distinct viruses. Researchers attempting to develop new antiviral therapies for DNA virus infections ... ...

    Abstract The search for new compounds with a broad spectrum of antiviral activity is important and requires the evaluation of many compounds against several distinct viruses. Researchers attempting to develop new antiviral therapies for DNA virus infections currently use a variety of cell lines, assay conditions and measurement methods to determine in vitro drug efficacy, making it difficult to compare results from within the same laboratory as well as between laboratories. In this paper, we describe the assessment of antiviral activity of a set of nucleoside analogs against BK polyomavirus, JC polyomavirus, Epstein-Barr virus, human herpesvirus 6B, and human herpesvirus 8 in an automated 384-well format and utilize qPCR assays to measure the accumulation of viral DNA. In an accompanying paper, we present a standardized approach to evaluating antivirals against additional herpesviruses, orthopoxviruses, and adenovirus. Together, they reveal new activities for reference compounds and help to define the spectrum of antiviral activity for a set of nucleoside analogs against a set of 12 DNA viruses that infect humans including representative human herpesviruses, orthopoxviruses, adenoviruses, and polyomaviruses. This analysis helps provide perspective on combinations of agents that would help provide broad coverage of significant pathogens in immunocompromised patients as well as against emerging infections.
    MeSH term(s) Antiviral Agents/pharmacology ; Automation, Laboratory ; DNA, Viral/analysis ; Drug Discovery/methods ; Drug Discovery/standards ; Herpesviridae/drug effects ; Humans ; Nucleosides/pharmacology ; Polyomavirus/drug effects ; Virus Replication/drug effects
    Chemical Substances Antiviral Agents ; DNA, Viral ; Nucleosides
    Language English
    Publishing date 2018-10-01
    Publishing country Netherlands
    Document type Evaluation Study ; Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 306628-9
    ISSN 1872-9096 ; 0166-3542
    ISSN (online) 1872-9096
    ISSN 0166-3542
    DOI 10.1016/j.antiviral.2018.09.016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: An acyclic phosphonate prodrug of HPMPC is effective against VZV in skin organ culture and mice.

    Lloyd, M G / Liu, D / Lyu, J / Fan, J / Overhulse, J M / Kashemirov, B A / Prichard, M N / McKenna, C E / Moffat, J F

    Antiviral research

    2022  Volume 199, Page(s) 105275

    Abstract: Varicella zoster virus (VZV) causes chicken pox and shingles and is prevalent worldwide. Acyclovir and penciclovir (and its prodrugs) are first-line treatments for VZV infections, but they are not highly potent against VZV and resistance may arise in ... ...

    Abstract Varicella zoster virus (VZV) causes chicken pox and shingles and is prevalent worldwide. Acyclovir and penciclovir (and its prodrugs) are first-line treatments for VZV infections, but they are not highly potent against VZV and resistance may arise in immunocompromised people on long-term therapy. HPMPC (cidofovir) is active against VZV, but cidofovir is not approved for treating VZV diseases, is nephrotoxic, and is not orally bioavailable. Here, we present the synthesis and evaluation of USC-373, a phosphonate prodrug of HPMPC with activity against VZV and other DNA viruses. In cultured fibroblasts, it was potent against VZV Ellen laboratory strain and was not overtly toxic, with EC
    MeSH term(s) Acyclovir/pharmacology ; Animals ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Cidofovir/pharmacology ; Herpesvirus 3, Human ; Humans ; Mice ; Organ Culture Techniques ; Organophosphonates/pharmacology ; Prodrugs/pharmacology ; Viral Regulatory and Accessory Proteins
    Chemical Substances Antiviral Agents ; ORF57 protein, human herpesvirus 8 ; Organophosphonates ; Prodrugs ; Viral Regulatory and Accessory Proteins ; Cidofovir (JIL713Q00N) ; Acyclovir (X4HES1O11F)
    Language English
    Publishing date 2022-03-03
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 306628-9
    ISSN 1872-9096 ; 0166-3542
    ISSN (online) 1872-9096
    ISSN 0166-3542
    DOI 10.1016/j.antiviral.2022.105275
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Neoplasia Diagnosis After Multi-target Stool DNA Is Enhanced Among Lowest Baseline Detectors.

    Ebner, Derek W / Burger, Kelli N / Mahoney, Douglas W / Broderick, Brendan T / Eckmann, Jason D / Devens, Mary E / Lowrie, Kari L / League, John B / Bering, Jamie / Kahn, Allon / Rodriguez, Eduardo A / Prichard, David O / Wallace, Michael B / Kane, Sunanda V / Leighton, Jonathan A / Buttar, Navtej S / Rutten, Lila J Finney / Gurudu, Suryakanth R / Kisiel, John B

    Digestive diseases and sciences

    2023  Volume 68, Issue 9, Page(s) 3721–3731

    Abstract: Background and aims: Variation in colorectal neoplasia detection limits the effectiveness of screening colonoscopy. By evaluating neoplasia detection rates of individual colonoscopists, we aimed to quantify the effects of pre-procedural knowledge of a ... ...

    Abstract Background and aims: Variation in colorectal neoplasia detection limits the effectiveness of screening colonoscopy. By evaluating neoplasia detection rates of individual colonoscopists, we aimed to quantify the effects of pre-procedural knowledge of a positive (+) multi-target stool DNA (mt-sDNA) on colonoscopy quality metrics.
    Methods: We retrospectively identified physicians who performed a high volume of + mt-sDNA colonoscopies; colorectal neoplasia at post-mt-sDNA colonoscopy was recorded. These colonoscopists were stratified into quartiles based on baseline adenoma detection rates. Baseline colonoscopy adenoma detection rates and sessile serrated lesion detection rates were compared to post-mt-sDNA colonoscopy neoplasia diagnosis rates among each quartile. Withdrawal times were measured from negative exams.
    Results: During the study period (2014-17) the highest quartile of physicians by volume of post-mt-sDNA colonoscopies were evaluated. Among thirty-five gastroenterologists, their median screening colonoscopy adenoma detection rate was 32% (IQR, 28-39%) and serrated lesion detection rate was 13% (8-15%). After + mt-sDNA, adenoma diagnosis increased to 47% (36-56%) and serrated lesion diagnosis increased to 31% (17-42%) (both p < 0.0001). Median withdrawal time increased from 10 (7-13) to 12 (10-17) minutes (p < 0.0001) and was proportionate across quartiles. After + mt-sDNA, lower baseline detectors had disproportionately higher rates of adenoma diagnosis in female versus male patients (p = 0.048) and higher serrated neoplasia diagnosis rates among all patients (p = 0.0092).
    Conclusions: Knowledge of + mt-sDNA enriches neoplasia diagnosis compared to average risk screening exams. Adenomatous and serrated lesion diagnosis was magnified among those with lower adenoma detection rates. Awareness of the mt-sDNA result may increase physician attention during colonoscopy. Pre-procedure knowledge of a positive mt-sDNA test improves neoplasia diagnosis rates among colonoscopists with lower baseline adenoma detection rates, independent of withdrawal time.
    MeSH term(s) Humans ; Male ; Female ; DNA, Neoplasm ; Retrospective Studies ; Early Detection of Cancer/methods ; Colonoscopy ; Colorectal Neoplasms/pathology ; Adenoma/pathology
    Chemical Substances DNA, Neoplasm
    Language English
    Publishing date 2023-07-24
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 304250-9
    ISSN 1573-2568 ; 0163-2116
    ISSN (online) 1573-2568
    ISSN 0163-2116
    DOI 10.1007/s10620-023-08038-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Synthesis of A-ring quinolones, nine-membered oxolactams and spiroindoles by oxidative transformations of 2,3-indolotriterpenoids.

    Khusnutdinova, Elmira F / Kazakova, Oxana B / Lobov, Alexander N / Kukovinets, Olga S / Suponitsky, Kyrill Yu / Meyers, Craig B / Prichard, Mark N

    Organic & biomolecular chemistry

    2018  Volume 17, Issue 3, Page(s) 585–597

    Abstract: This paper describes an access to new nitrogen-containing heterocyclic triterpenoids by the reaction of 2,3-indolotriterpenoids with ozone and dimethyldioxirane. The oxidation of indolo-fused 28-oxo-allobetulin or methyl platanoate with ozone led to a ... ...

    Abstract This paper describes an access to new nitrogen-containing heterocyclic triterpenoids by the reaction of 2,3-indolotriterpenoids with ozone and dimethyldioxirane. The oxidation of indolo-fused 28-oxo-allobetulin or methyl platanoate with ozone led to a mixture of a quinolone as the major product and a nine-membered 2,3-seco-2-oxolactam and three different types of spiroindoles as byproducts. The formation of quinolone and 2,3-seco-2-oxolactam derivatives could be explained by the standard 1,3-dipolar cycloaddition of ozone to the C2(3)-double bond of the triterpene core similar to the products observed in the ozonolysis of indoles in the Witkop-Winterfeldt oxidation (WWO). The formation of spiroindoles was unexpected and could be explained through the 1,2-cycloaddition of ozone to the C2(3)-double bond with consecutive intramolecular rearrangements of the 2,3-epoxy-intermediate. These spiroindoles seem to be novel structures observed in the WWO reaction. The formation of only two isomeric triterpene spiroindolinones was achieved by the oxidation of 2,3-indolo-28-oxo-allobetulin with dimethyldioxirane that could be explained by the rearrangement of the 2,3-epoxy-intermediate. 19β,28-Epoxy-18α-olean-28-oxo-2-nor-2,3-4'(1H)-quinolone was the most active against HPV-11 with EC50 0.45 μM and SI50 322 in a primary assay and SI90 < 10 against HPV-16 in a secondary assay. The oxidative transformations of indolotriterpenoids have great potential for further modifications towards the preparation of new biologically active compounds.
    Language English
    Publishing date 2018-12-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2097583-1
    ISSN 1477-0539 ; 1477-0520
    ISSN (online) 1477-0539
    ISSN 1477-0520
    DOI 10.1039/c8ob02624f
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Helicase-primase as a target of new therapies for herpes simplex virus infections.

    James, S H / Larson, K B / Acosta, E P / Prichard, M N

    Clinical pharmacology and therapeutics

    2015  Volume 97, Issue 1, Page(s) 66–78

    Abstract: The seminal discovery of acyclovir 40 years ago heralded the modern era of truly selective antiviral therapies and this drug remains the therapy of choice for herpes simplex virus infections. Yet by modern standards, its antiviral activity is modest and ... ...

    Abstract The seminal discovery of acyclovir 40 years ago heralded the modern era of truly selective antiviral therapies and this drug remains the therapy of choice for herpes simplex virus infections. Yet by modern standards, its antiviral activity is modest and new drugs against novel molecular targets such as the helicase-primase have the potential to improve clinical outcome, particularly in high-risk patients. A brief synopsis of current therapies for these infections and clinical need is provided to help provide an initial perspective. The function of the helicase-primase complex is then summarized and the development of new inhibitors of the helicase-primase complex, such as pritelivir and amenamevir, is discussed. We review their mechanism of action, propensity for drug resistance, and pharmacokinetic characteristics and discuss their potential to advance current therapeutic options. Strategies that include combinations of these inhibitors with acyclovir are also considered, as they will likely maximize clinical efficacy.
    MeSH term(s) Acyclovir/pharmacology ; Acyclovir/therapeutic use ; Animals ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; DNA Helicases/antagonists & inhibitors ; DNA Primase/antagonists & inhibitors ; Drug Design ; Drug Resistance, Viral ; Herpes Simplex/drug therapy ; Herpes Simplex/virology ; Humans ; Molecular Targeted Therapy ; Oxadiazoles/pharmacology ; Oxadiazoles/therapeutic use ; Pyridines/pharmacology ; Pyridines/therapeutic use ; Thiazoles/pharmacology ; Thiazoles/therapeutic use ; Viral Proteins/antagonists & inhibitors
    Chemical Substances ASP2151 ; Antiviral Agents ; BAY 57-1293 ; Oxadiazoles ; Pyridines ; Thiazoles ; Viral Proteins ; DNA Primase (EC 2.7.7.-) ; helicase-primase, Human herpesvirus 1 (EC 3.1.-) ; DNA Helicases (EC 3.6.4.-) ; Acyclovir (X4HES1O11F)
    Language English
    Publishing date 2015-01
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 123793-7
    ISSN 1532-6535 ; 0009-9236
    ISSN (online) 1532-6535
    ISSN 0009-9236
    DOI 10.1002/cpt.3
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  7. Article ; Online: Progress in the development of new therapies for herpesvirus infections.

    Price, Nathan B / Prichard, Mark N

    Current opinion in virology

    2009  Volume 1, Issue 6, Page(s) 548–554

    Abstract: Resurgent interest in antiviral drugs for the treatment of herpesvirus has led to the development of new compounds that are progressing through clinical trials. This is important because there are few therapeutic options for resistant infections and some ...

    Abstract Resurgent interest in antiviral drugs for the treatment of herpesvirus has led to the development of new compounds that are progressing through clinical trials. This is important because there are few therapeutic options for resistant infections and some viruses such as human cytomegalovirus remain underserved. New compounds include conventional DNA polymerase inhibitors such as valomaciclovir and cyclopropavir, as well as CMX001 that has a broad spectrum of antiviral activity that includes all the herpesviruses. It also includes compounds with new molecular targets such as maribavir (MBV), FV-100, AIC361, and AIC246. Recent advances with each of these compounds will be reviewed including their virus specificity, mechanism of action, and stage of development. The potential of these new compounds to improve clinical outcome will also be discussed.
    MeSH term(s) Animals ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Clinical Trials as Topic ; Herpesviridae/isolation & purification ; Herpesviridae Infections/drug therapy ; Herpesviridae Infections/virology ; Humans
    Chemical Substances Antiviral Agents
    Language English
    Publishing date 2009-12-09
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2611378-8
    ISSN 1879-6265 ; 1879-6257
    ISSN (online) 1879-6265
    ISSN 1879-6257
    DOI 10.1016/j.coviro.2011.10.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Commercially Available Apps to Support Healthy Family Meals: User Testing of App Utility, Acceptability, and Engagement.

    Mauch, Chelsea E / Laws, Rachel A / Prichard, Ivanka / Maeder, Anthony J / Wycherley, Thomas P / Golley, Rebecca K

    JMIR mHealth and uHealth

    2021  Volume 9, Issue 5, Page(s) e22990

    Abstract: ... Methods: Working parents (N=133) were recruited via the web and completed a 10-item Capability ... Opportunity, Motivation, and Behavior (COM-B) self-evaluation survey assessing their needs in relation ... Survey participants (N=133; mean age 34 years, SD 4 years) were mainly mothers (130/133, 97.7%) and ...

    Abstract Background: Parents juggling caregiving and paid employment encounter a range of barriers in providing healthy food to their families. Mobile apps have the potential to help parents in planning, purchasing, and preparing healthy family food. The utility and acceptability of apps for supporting parents are unknown. User perspectives of existing technology, such as commercially available apps, can guide the development of evidence-based apps in the future.
    Objective: This study aims to determine the feasibility of existing commercially available apps for supporting the healthy food provision practices of working parents.
    Methods: Working parents (N=133) were recruited via the web and completed a 10-item Capability, Opportunity, Motivation, and Behavior (COM-B) self-evaluation survey assessing their needs in relation to the provision of healthy family meals. A total of 5 apps were selected for testing, including a meal planning app, recipe app, recipe manager app, family organizer app, and barcode scanning app. Survey items were mapped to app features, with a subsample of parents (67/133, 50.4%) allocated 2 apps each to trial simultaneously over 4 weeks. A semistructured interview exploring app utility and acceptability and a web-based survey, including the System Usability Scale and the user version of the Mobile App Rating Scale, followed app testing. The interview data were analyzed using a theoretical thematic approach.
    Results: Survey participants (N=133; mean age 34 years, SD 4 years) were mainly mothers (130/133, 97.7%) and partnered (122/133, 91.7%). Participants identified a need for healthy recipes (109/133, 82% agreed or strongly agreed) and time for food provision processes (107/133, 80.5%). Engagement quality was the lowest rated domain of the user version of the Mobile App Rating Scale across all 5 apps (mean score per app ranging from 3.0 to 3.7 out of a maximum of 5). The family organizer, requiring a high level of user input, was rated the lowest for usability (median 48, IQR 34-73). In the interviews, participants weighed the benefits of the apps (ie, time saving) against the effort involved in using them in determining their acceptability. Organization was a subtheme emerging from interviews, associated with the use of meal planners and shopping lists. Meal planners and shopping lists were used in time, while behavior was occurring.
    Conclusions: Meal planning apps and features promoting organization present feasible, time-saving solutions to support healthy food provision practices. Attention must be paid to enhancing app automation and integration, as well as recipe and nutrition content, to ensure that apps do not add to the time burden of food provision and are supportive of healthy food provision behavior in time.
    MeSH term(s) Adult ; Consumer Behavior ; Health Behavior ; Humans ; Meals ; Mobile Applications ; Surveys and Questionnaires
    Language English
    Publishing date 2021-05-07
    Publishing country Canada
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2719220-9
    ISSN 2291-5222 ; 2291-5222
    ISSN (online) 2291-5222
    ISSN 2291-5222
    DOI 10.2196/22990
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  9. Article: The second Lilly Prize Lecture, University of Newcastle, July 1977. beta-Adrenergic receptor blockade in hypertension, past, present and future. 1978.

    Prichard, B N C

    British journal of clinical pharmacology

    2004  Volume 58, Issue 7, Page(s) S807–27; discussion S828–30

    MeSH term(s) Adrenergic beta-Antagonists/history ; Adrenergic beta-Antagonists/therapeutic use ; Awards and Prizes ; History, 20th Century ; Humans ; Hypertension/drug therapy ; Hypertension/history
    Chemical Substances Adrenergic beta-Antagonists
    Language English
    Publishing date 2004-12
    Publishing country England
    Document type Biography ; Classical Article ; Historical Article ; Journal Article
    ZDB-ID 188974-6
    ISSN 1365-2125 ; 0306-5251 ; 0264-3774
    ISSN (online) 1365-2125
    ISSN 0306-5251 ; 0264-3774
    DOI 10.1111/j.1365-2125.2004.02302.x
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  10. Article ; Online: Detection of Postcolonoscopy Colorectal Neoplasia by Multi-target Stool DNA.

    Ebner, Derek W / Eckmann, Jason D / Burger, Kelli N / Mahoney, Douglas W / Bering, Jamie / Kahn, Allon / Rodriguez, Eduardo A / Prichard, David O / Wallace, Michael B / Kane, Sunanda V / Finney Rutten, Lila J / Gurudu, Suryakanth R / Kisiel, John B

    Clinical and translational gastroenterology

    2021  Volume 12, Issue 6, Page(s) e00375

    Abstract: Introduction: Significant variability between colonoscopy operators contributes to postcolonoscopy colorectal cancers (CRCs). We aimed to estimate postcolonoscopy colorectal neoplasia (CRN) detection by multi-target stool DNA (mt-sDNA), which has not ... ...

    Abstract Introduction: Significant variability between colonoscopy operators contributes to postcolonoscopy colorectal cancers (CRCs). We aimed to estimate postcolonoscopy colorectal neoplasia (CRN) detection by multi-target stool DNA (mt-sDNA), which has not previously been studied for this purpose.
    Methods: In a retrospective cohort of patients with +mt-sDNA and completed follow-up colonoscopy, positive predictive value (PPV) for endpoints of any CRN, advanced adenoma, right-sided neoplasia, sessile serrated polyps (SSP), and CRC were stratified by the time since previous colonoscopy (0-9, 10, and ≥11 years). mt-sDNA PPV at ≤9 years from previous average-risk screening colonoscopy was used to estimate CRN missed at previous screening colonoscopy.
    Results: Among the 850 studied patients with +mt-sDNA after a previous negative screening colonoscopy, any CRN was found in 535 (PPV 63%). Among 107 average-risk patients having +mt-sDNA ≤9 years after last negative colonoscopy, any CRN was found in 67 (PPV 63%), advanced neoplasia in 16 (PPV 15%), right-sided CRN in 48 (PPV 46%), and SSP in 20 (PPV 19%). These rates were similar to those in 47 additional average risk persons with previous incomplete colonoscopy and in an additional 68 persons at increased CRC risk. One CRC (stage I) was found in an average risk patient who was mt-sDNA positive 6 years after negative screening colonoscopy.
    Discussion: The high PPV of mt-sDNA 0-9 years after a negative screening colonoscopy suggests that lesions were likely missed on previous examination or may have arisen de novo. mt-sDNA as an interval test after negative screening colonoscopy warrants further study.
    MeSH term(s) Adenoma/diagnosis ; Aged ; Colonoscopy ; Colorectal Neoplasms/diagnosis ; DNA, Neoplasm/analysis ; Early Detection of Cancer ; Feces/chemistry ; Female ; Humans ; Male ; Mass Screening/methods ; Middle Aged ; Precancerous Conditions/diagnosis ; Predictive Value of Tests ; Retrospective Studies
    Chemical Substances DNA, Neoplasm
    Language English
    Publishing date 2021-06-18
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2581516-7
    ISSN 2155-384X ; 2155-384X
    ISSN (online) 2155-384X
    ISSN 2155-384X
    DOI 10.14309/ctg.0000000000000375
    Database MEDical Literature Analysis and Retrieval System OnLINE

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