LIVIVO - Search results -

Search results

Result 1 - 10 of total 259

Search options

Article ; Online: Fully Aqueous Self-Assembly of a Gold-Nanoparticle-Based Pathogen Sensor.

Robson, Timothy / Shah, Deepan S H / Welbourn, Rebecca J L / Phillips, Sion R / Clifton, Luke A / Lakey, Jeremy H

International journal of molecular sciences

2023 Volume 24, Issue 8

Abstract: Surface plasmon resonance (SPR) is a very sensitive measure of biomolecular interactions but is generally too expensive for routine analysis of clinical samples. Here we demonstrate the simplified formation of virus-detecting gold nanoparticle (AuNP) ... ...

Abstract	Surface plasmon resonance (SPR) is a very sensitive measure of biomolecular interactions but is generally too expensive for routine analysis of clinical samples. Here we demonstrate the simplified formation of virus-detecting gold nanoparticle (AuNP) assemblies on glass using only aqueous buffers at room temperature. The AuNP assembled on silanized glass and displayed a distinctive absorbance peak due to the localized SPR (LSPR) response of the AuNPs. Next, assembly of a protein engineering scaffold was followed using LSPR and a sensitive neutron reflectometry approach, which measured the formation and structure of the biological layer on the spherical AuNP. Finally, the assembly and function of an artificial flu sensor layer consisting of an in vitro-selected single-chain antibody (scFv)-membrane protein fusion was followed using the LSPR response of AuNPs within glass capillaries. In vitro selection avoids the need for separate animal-derived antibodies and allows for the rapid production of low-cost sensor proteins. This work demonstrates a simple approach to forming oriented arrays of protein sensors on nanostructured surfaces that uses (i) an easily assembled AuNP silane layer, (ii) self-assembly of an oriented protein layer on AuNPs, and (iii) simple highly specific artificial receptor proteins.
MeSH term(s)	Animals ; Gold/chemistry ; Metal Nanoparticles/chemistry ; Surface Plasmon Resonance ; Antibodies ; Membrane Proteins
Chemical Substances	Gold (7440-57-5) ; Antibodies ; Membrane Proteins
Language	English
Publishing date	2023-04-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24087599
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Behavior of carbon monoxide, nitrogen oxides, and ozone in a vehicle cabin with a passenger.

Mendez-Jimenez, David / Lakey, Pascale S J / Shiraiwa, Manabu / Jung, Heejung

Environmental science. Processes & impacts

2021 Volume 23, Issue 2, Page(s) 302–310

Abstract: Drivers and passengers are exposed to high concentrations of air pollutants while driving. While there are many studies to assess exposure to air pollutants penetrating into a vehicle cabin, little is known about how individual gas pollutants are ... ...

Abstract	Drivers and passengers are exposed to high concentrations of air pollutants while driving. While there are many studies to assess exposure to air pollutants penetrating into a vehicle cabin, little is known about how individual gas pollutants are behaving (e.g. accumulating, depositing, reacting etc.) in the cabin. This study investigated the characteristic behavior of CO, NO, NO2 and O3 in a vehicle cabin in the presence of a driver with static, pseudo dynamic and dynamic tests. We found in our experiments that CO and NO concentrations increased while O3 and NO2 concentrations decreased rapidly when cabin air was recirculated. A kinetic model, which contains 20 chemical reactions, could predict the static test results well. CO and NO accumulations in the cabin were due to exhalation from the driver and conversion of NO2 to NO upon deposition to surfaces may also play a role. Pseudo dynamic and dynamic test results showed similar results. During the fresh air mode CO, NO, and NO2 followed similar trends between the inside and outside of the cabin, while in cabin O3 concentrations were lower compared to outside concentrations due to reactions with the human and surface deposition. The Cabin Air Quality Index approached 0.8 and 0.4 for O3 during pseudo dynamic and dynamic tests, respectively. Accumulation of NO in the cabin was not obvious during the dynamic test due to a large variation of outside NO concentrations. We encourage auto manufacturers to develop control algorithms and devices to reduce a passenger's exposure to gaseous pollutants in vehicle cabins.
MeSH term(s)	Air Pollutants/analysis ; Air Pollution/analysis ; Carbon Monoxide/analysis ; Environmental Monitoring ; Humans ; Nitric Oxide ; Nitrogen Oxides/analysis ; Ozone/analysis
Chemical Substances	Air Pollutants ; Nitrogen Oxides ; Nitric Oxide (31C4KY9ESH) ; Ozone (66H7ZZK23N) ; Carbon Monoxide (7U1EE4V452)
Language	English
Publishing date	2021-01-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2703814-2
ISSN	2050-7895 ; 2050-7887
ISSN (online)	2050-7895
ISSN	2050-7887
DOI	10.1039/d0em00395f
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 7401: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The effect of built-in and portable ionizers on in-cabin ozone concentrations in light-duty vehicles.

Mendez-Jimenez, David / Lakey, Pascale S J / Johnson, Grace / Shiraiwa, Manabu / Jung, Heejung

Environmental science. Processes & impacts

2022 Volume 24, Issue 10, Page(s) 1725–1734

Abstract: This paper investigates the effects of ionizers on the ozone concentration within vehicle cabins by using a series of measurements combined with a kinetic box model. Testing consisted of measuring ozone concentration during static tests where the ... ...

Abstract	This paper investigates the effects of ionizers on the ozone concentration within vehicle cabins by using a series of measurements combined with a kinetic box model. Testing consisted of measuring ozone concentration during static tests where the ventilation of the test vehicle was turned on and off depending on the test. This testing was repeated for three different portable ionizers and two vehicles with built-in ionizers. Ionizer A produced ozone at a rate of ∼0.04 ppb s
MeSH term(s)	Ozone/analysis ; Ventilation ; Air Pollution ; Air Pollutants/analysis ; Air Pollution, Indoor/analysis
Chemical Substances	Ozone (66H7ZZK23N) ; Air Pollutants
Language	English
Publishing date	2022-10-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2703814-2
ISSN	2050-7895 ; 2050-7887
ISSN (online)	2050-7895
ISSN	2050-7887
DOI	10.1039/d2em00129b
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 7401: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Chemical and Cellular Formation of Reactive Oxygen Species from Secondary Organic Aerosols in Epithelial Lining Fluid.

Shiraiwa, M / Fang, T / Wei, J / Lakey, Psj / Hwang, Bch / Edwards, K C / Kapur, S / Mena, Jem / Huang, Y-K / Digman, M A / Weichenthal, S A / Nizkorodov, S / Kleinman, M T

Research report (Health Effects Institute)

2024 , Issue 215, Page(s) 1–56

Abstract: ... H: Quinones and isoprene SOA activated nicotinamide adenine dinucleotide phosphate (NADPH) oxidase ...

Abstract	Introduction: Oxidative stress mediated by reactive oxygen species (ROS) is a key process for adverse aerosol health effects. Secondary organic aerosols (SOA) account for a major fraction of particulate matter with aerodynamic diameter ≤2.5 µm (PM Methods: SOA particles were generated using reaction chambers from oxidation of various precursors including isoprene, terpenes, and aromatic compounds with or without nitrogen oxides (NO Results: Superoxide radicals (·O Organic radicals in the ELF were formed by mixtures of Fe Wildfire PM mainly generated ·OH and R· with minor contributions from superoxide and oxygen-centered organic radicals (RO·). PM OP was high in wildfire PM, exhibiting very weak correlation with radical forms of ROS. These results were in stark contrast with PM collected at highway and urban sites, which generated much higher amounts of radicals dominated by ·OH radicals that correlated well with OP. By combining field measurements of size-segregated chemical composition, a human respiratory tract model, and kinetic modeling, we quantified production rates and concentrations of different types of ROS in different regions of the ELF by considering particle-size-dependent respiratory deposition. While hydrogen peroxide (H Quinones and isoprene SOA activated nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in macrophages, releasing massive amounts of superoxide via respiratory burst and overwhelming the superoxide formation by aqueous chemical reactions in the ELF. The threshold dose for macrophage activation was much smaller for quinones compared with isoprene SOA. The released ROS caused lipid peroxidation to increase cell membrane fluidity, inducing oxidative damage and stress. Further increases of doses led to the activation of antioxidant response elements, reducing the net cellular superoxide production. At very high doses and long exposure times, chemical production became comparably important or dominant if the escalation of oxidative stress led to cell death. Conclusions: The mechanistic understandings and quantitative information on ROS generation by SOA particles provided a basis for further elucidation of adverse aerosol health effects and oxidative stress by PM
MeSH term(s)	Humans ; Reactive Oxygen Species/metabolism ; Air Pollutants ; Hydrogen Peroxide ; Superoxides ; Particulate Matter/metabolism ; Aerosols/metabolism ; Hydroxyl Radical ; Organic Chemicals ; Quinones ; Water ; Cyclohexane Monoterpenes ; Butadienes ; Hemiterpenes
Chemical Substances	Reactive Oxygen Species ; isoprene (0A62964IBU) ; alpha-terpineol (21334LVV8W) ; Air Pollutants ; Hydrogen Peroxide (BBX060AN9V) ; Superoxides (11062-77-4) ; Particulate Matter ; Aerosols ; Hydroxyl Radical (3352-57-6) ; Organic Chemicals ; Quinones ; Water (059QF0KO0R) ; Cyclohexane Monoterpenes ; Butadienes ; Hemiterpenes
Language	English
Publishing date	2024-02-29
Publishing country	United States
Document type	Journal Article
ISSN	1041-5505
ISSN	1041-5505
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior.

Le Bao, Chau / Waller, Helen / Dellaquila, Alessandra / Peters, Daniel / Lakey, Jeremy / Chaubet, Frédéric / Simon-Yarza, Teresa

International journal of molecular sciences

2022 Volume 23, Issue 23

Abstract: In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function. The ...

Abstract	In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function. The microenvironment of the ECM plays a fundamental role in regulating angiogenesis. Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems. The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior. Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF). Hydrogel functionalization was achieved by electrostatic interactions via incorporation of diethylaminoethyl (DEAE)-dextran. Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules. Cells in functionalized scaffolds survived, adhered, and proliferated over seven days. When incorporated alone, Caf1-YIGSR mainly induced cell adhesion and proliferation, whereas Caf1-VEGF promoted cell migration and sprouting. Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective. This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.
Language	English
Publishing date	2022-11-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms232314604
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Partitioning of reactive oxygen species from indoor surfaces to indoor aerosols.

Morrison, Glenn C / Eftekhari, Azin / Lakey, Pascale S J / Shiraiwa, Manabu / Cummings, Bryan E / Waring, Michael S / Williams, Brent

Environmental science. Processes & impacts

2022

Abstract: ... PM is from ozone-surface chemistry. At an air change rate equal to 1/h, and an outdoor ozone mixing ... to PM. For the same conditions, but with a modest indoor source of PM (1.5 mg h ...

Abstract	Reactive oxygen species (ROS) are among the species thought to be responsible for the adverse health effects of particulate matter (PM) inhalation. Field studies suggest that indoor sources of ROS contribute to measured ROS on PM in indoor air. We hypothesize that ozone reacts on indoor surfaces to form semi-volatile ROS, in particular organic peroxides (OPX), which partition to airborne particles. To test this hypothesis, we modeled ozone-induced formation of OPX, its decay and its partitioning to PM in a residential building and compared the results to field measurements. Simulations indicate that, while ROS of outdoor origin is the primary contributor to indoor ROS (in PM), a substantial fraction of ROS present in indoor PM is from ozone-surface chemistry. At an air change rate equal to 1/h, and an outdoor ozone mixing ratio of 35 ppb, 25% of the ROS concentration in air is due to indoor formation and partitioning of OPX to PM. For the same conditions, but with a modest indoor source of PM (1.5 mg h
Language	English
Publishing date	2022-10-31
Publishing country	England
Document type	Journal Article
ZDB-ID	2703814-2
ISSN	2050-7895 ; 2050-7887
ISSN (online)	2050-7895
ISSN	2050-7887
DOI	10.1039/d2em00307d
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 7401: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Oxidative Potential of Particulate Matter and Generation of Reactive Oxygen Species in Epithelial Lining Fluid.

Fang, Ting / Lakey, Pascale S J / Weber, Rodney J / Shiraiwa, Manabu

Environmental science & technology

2019 Volume 53, Issue 21, Page(s) 12784–12792

Abstract: ... to have higher ROS concentrations compared to the bronchial and alveolar regions. Although H ...

Abstract	Reactive oxygen species (ROS) play a central role in adverse health effects of atmospheric particulate matter (PM). Respiratory deposition can lead to the formation of ROS in the epithelial lining fluid due to redox reactions of PM components with lung antioxidants. As direct quantification of ROS is challenging, PM oxidative potential is more commonly measured using antioxidant surrogates including dithiothreitol and ascorbic acid, assuming that the decay of surrogates corresponds to ROS formation. However, this assumption has not yet been validated and the lack of ROS quantification in the respiratory tract causes major limitations in evaluating PM impacts on oxidative stress. By combining field measurements of size-segregated chemical composition, a human respiratory tract model, and kinetic modeling, we quantified production rates and concentrations of different types of ROS in different regions of the epithelial lining fluid by considering particle-size-dependent respiratory deposition. The extrathoracic region is found to have higher ROS concentrations compared to the bronchial and alveolar regions. Although H
MeSH term(s)	Air Pollutants ; Humans ; Hydrogen Peroxide ; Oxidation-Reduction ; Oxidative Stress ; Particulate Matter ; Reactive Oxygen Species
Chemical Substances	Air Pollutants ; Particulate Matter ; Reactive Oxygen Species ; Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2019-10-11
Publishing country	United States
Document type	Journal Article
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.9b03823
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: SARS-CoV-2 Serostatus and COVID-19 Illness Characteristics by Variant Time Period in Non-Hospitalized Children and Adolescents.

Messiah, Sarah E / Swartz, Michael D / Abbas, Rhiana A / Talebi, Yashar / Kohl, Harold W / Valerio-Shewmaker, Melissa / DeSantis, Stacia M / Yaseen, Ashraf / Kelder, Steven H / Ross, Jessica A / Padilla, Lindsay N / Gonzalez, Michael O / Wu, Leqing / Lakey, David / Shuford, Jennifer A / Pont, Stephen J / Boerwinkle, Eric

Children (Basel, Switzerland)

2023 Volume 10, Issue 5

Abstract: Objective: To describe COVID-19 illness characteristics, risk factors, and SARS-CoV-2 serostatus by variant time period in a large community-based pediatric sample.: Design: Data were collected prospectively over four timepoints between October 2020 ... ...

Abstract	Objective: To describe COVID-19 illness characteristics, risk factors, and SARS-CoV-2 serostatus by variant time period in a large community-based pediatric sample. Design: Data were collected prospectively over four timepoints between October 2020 and November 2022 from a population-based cohort ages 5 to 19 years old. Setting: State of Texas, USA. Participants: Participants ages 5 to 19 years were recruited from large pediatric healthcare systems, Federally Qualified Healthcare Centers, urban and rural clinical practices, health insurance providers, and a social media campaign. Exposure: SARS-CoV-2 infection. Main outcome(s) and measure(s): SARS-CoV-2 antibody status was assessed by the Roche Elecsys Results: Over half (57.2%) of the sample (N = 3911) was antibody positive. Symptomatic infection increased over time from 47.09% during the pre-Delta variant time period, to 76.95% during Delta, to 84.73% during Omicron, and to 94.79% during the Omicron BA.2. Those who were not vaccinated were more likely (OR 1.71, 95% CI 1.47, 2.00) to be infected versus those fully vaccinated. Conclusions: Results show an increase in symptomatic COVID-19 infection among non-hospitalized children with each progressive variant over the past two years. Findings here support the public health guidance that eligible children should remain up to date with COVID-19 vaccinations.
Language	English
Publishing date	2023-04-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2732685-8
ISSN	2227-9067
ISSN	2227-9067
DOI	10.3390/children10050818
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Cl-out is a novel cooperative optogenetic tool for extruding chloride from neurons.

Alfonsa, Hannah / Lakey, Jeremy H / Lightowlers, Robert N / Trevelyan, Andrew J

Nature communications

2016 Volume 7, Page(s) 13495

Abstract: Chloride regulation affects brain function in many ways, for instance, by dictating the GABAergic reversal potential, and thereby influencing neuronal excitability and spike timing. Consistent with this, there is increasing evidence implicating chloride ... ...

Abstract	Chloride regulation affects brain function in many ways, for instance, by dictating the GABAergic reversal potential, and thereby influencing neuronal excitability and spike timing. Consistent with this, there is increasing evidence implicating chloride in a range of neurological conditions. Investigations about these conditions, though, are made difficult by the limited range of tools available to manipulate chloride levels. In particular, there has been no way to actively remove chloride from neurons; we now describe an optogenetic strategy, 'Cl-out', to do exactly this. Cl-out achieves its effect by the cooperative action of two different component opsins: the proton pump, Archaerhodopsin and a chloride channel opsin. The removal of chloride happens when both are activated together, using Archaerhodopsin as an optical voltage clamp to provide the driving force for chloride removal through the concurrently opened, chloride channels. We further show that this novel optogenetic strategy can reverse an in vitro epileptogenic phenotype.
MeSH term(s)	Animals ; Chloride Channels/physiology ; Chlorides/chemistry ; Embryo, Mammalian ; Female ; Gramicidin ; Male ; Membrane Potentials ; Neurons/chemistry ; Neurons/physiology ; Optogenetics/methods ; Patch-Clamp Techniques ; Rats
Chemical Substances	Chloride Channels ; Chlorides ; Gramicidin (1405-97-6)
Language	English
Publishing date	2016-11-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/ncomms13495
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: A molecular picture of surface interactions of organic compounds on prevalent indoor surfaces: limonene adsorption on SiO

Fang, Yuan / Lakey, Pascale S J / Riahi, Saleh / McDonald, Andrew T / Shrestha, Mona / Tobias, Douglas J / Shiraiwa, Manabu / Grassian, Vicki H

Chemical science

2019 Volume 10, Issue 10, Page(s) 2906–2914

Abstract: Indoor surfaces are often coated with organic compounds yet a molecular understanding of what drives these interactions is poorly understood. Herein, the adsorption and desorption of limonene, an organic compound found in indoor environments, on ... ...

Abstract	Indoor surfaces are often coated with organic compounds yet a molecular understanding of what drives these interactions is poorly understood. Herein, the adsorption and desorption of limonene, an organic compound found in indoor environments, on hydroxylated silica (SiO
Language	English
Publishing date	2019-01-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2559110-1
ISSN	2041-6539 ; 2041-6520
ISSN (online)	2041-6539
ISSN	2041-6520
DOI	10.1039/c8sc05560b
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito