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Article ; Online: PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos.

Abbatemarco, Simona / Bondaz, Alexandra / Schwager, Francoise / Wang, Jing / Hammell, Christopher M / Gotta, Monica

2021 Volume 134, Issue 22

Abstract: When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here, we use C. elegans to study two proteins that are important for stress granule assembly in ... ...

Abstract	When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here, we use C. elegans to study two proteins that are important for stress granule assembly in human cells - PQN-59, the human UBAP2L ortholog, and GTBP-1, the human G3BP1 and G3BP2 ortholog. Both proteins assemble into stress granules in the embryo and in the germline when C. elegans is exposed to stressful conditions. Neither of the two proteins is essential for the assembly of stress-induced granules, as shown by the single and combined depletions by RNAi, and neither pqn-59 nor gtbp-1 mutant embryos show higher sensitivity to stress than control embryos. We find that pqn-59 mutants display reduced progeny and a high percentage of embryonic lethality, phenotypes that are not dependent on stress exposure and that are not shared with gtbp-1 mutants. Our data indicate that, in contrast to human cells, PQN-59 and GTBP-1 are not required for stress granule formation but that PQN-59 is important for C. elegans development.
MeSH term(s)	Animals ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans Proteins/genetics ; Carrier Proteins ; DNA Helicases ; Humans ; Poly-ADP-Ribose Binding Proteins ; RNA Helicases ; RNA Recognition Motif Proteins ; Stress Granules
Chemical Substances	Caenorhabditis elegans Proteins ; Carrier Proteins ; Poly-ADP-Ribose Binding Proteins ; RNA Recognition Motif Proteins ; Ubap2L protein, human ; DNA Helicases (EC 3.6.4.-) ; G3BP1 protein, human (EC 3.6.4.12) ; RNA Helicases (EC 3.6.4.13)
Language	English
Publishing date	2021-11-15
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2993-2
ISSN	1477-9137 ; 0021-9533
ISSN (online)	1477-9137
ISSN	0021-9533
DOI	10.1242/jcs.258834
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Zs.A 1200: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Inducing RNAi in Caenorhabditis elegans by Injection of dsRNA.

Hammell, Christopher M / Hannon, Gregory J

Cold Spring Harbor protocols

2016 Volume 2016, Issue 1, Page(s) pdb.prot086306

Abstract: In Caenorhabditis elegans, long double-stranded RNAs (dsRNAs) are overwhelmingly the trigger of choice for inducing RNA interference (RNAi). Although injection of dsRNA into the somatic or germline tissues of animals requires both specific equipment and ... ...

Abstract	In Caenorhabditis elegans, long double-stranded RNAs (dsRNAs) are overwhelmingly the trigger of choice for inducing RNA interference (RNAi). Although injection of dsRNA into the somatic or germline tissues of animals requires both specific equipment and technical skills, the ability of C. elegans to amplify the initial dsRNA trigger and to transmit the RNAi activity to other somatic tissues and to the progeny of injected animals is one of the main advantages of using C. elegans as a model system. The direct injection of dsRNA into parental animals is the most reliable method for RNAi and also presents the least experiment-to-experiment and animal-to-animal variability.
MeSH term(s)	Animals ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Microinjections ; RNA Interference/physiology ; RNA, Double-Stranded/administration & dosage ; RNA, Double-Stranded/metabolism
Chemical Substances	Caenorhabditis elegans Proteins ; RNA, Double-Stranded
Language	English
Publishing date	2016-01-04
Publishing country	United States
Document type	Journal Article
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot086306
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Article ; Online: Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for Groucho in regulating a proliferative-invasive cellular switch in

Medwig-Kinney, Taylor N / Kinney, Brian A / Martinez, Michael A Q / Yee, Callista / Sirota, Sydney S / Mullarkey, Angelina A / Somineni, Neha / Hippler, Justin / Zhang, Wan / Shen, Kang / Hammell, Christopher / Pani, Ariel M / Matus, David Q

eLife

2023 Volume 12

Abstract: A growing body of evidence suggests that cell division and basement membrane invasion are mutually exclusive cellular behaviors. How cells switch between proliferative and invasive states is not well understood. Here, we investigated this dichotomy in ... ...

Abstract	A growing body of evidence suggests that cell division and basement membrane invasion are mutually exclusive cellular behaviors. How cells switch between proliferative and invasive states is not well understood. Here, we investigated this dichotomy in vivo by examining two cell types in the developing
MeSH term(s)	Animals ; Basic Helix-Loop-Helix Transcription Factors/metabolism ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/metabolism ; Cell Differentiation ; Gene Expression Regulation ; Transcription Factors/metabolism ; Receptors, Cytoplasmic and Nuclear/metabolism
Chemical Substances	Basic Helix-Loop-Helix Transcription Factors ; Caenorhabditis elegans Proteins ; HLH-2 protein, C elegans ; Transcription Factors ; Nhr-67 protein, C elegans ; Receptors, Cytoplasmic and Nuclear ; unc-37 protein, C elegans
Language	English
Publishing date	2023-12-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.84355
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Article ; Online: American Medical Society of Sports Medicine Position Statement: Mononucleosis and Athletic Participation.

Putukian, Margot / McGrew, Christopher A / Benjamin, Holly J / Hammell, Mary Kitazono / Hwang, Calvin E / Ray, Jeremiah W / Statuta, Siobhan M / Sylvester, Jillian / Wilson, Kristina

Clinical journal of sport medicine : official journal of the Canadian Academy of Sport Medicine

2023

Abstract: Abstract: Infectious mononucleosis (IM) is a common illness in children and young adults caused primarily by the Epstein-Barr Virus (EBV). Transmission occurs primarily through sharing oral secretions, thus IM is known as the "kissing disease." Common ... ...

Abstract	Abstract: Infectious mononucleosis (IM) is a common illness in children and young adults caused primarily by the Epstein-Barr Virus (EBV). Transmission occurs primarily through sharing oral secretions, thus IM is known as the "kissing disease." Common clinical manifestations include fever, pharyngitis, posterior cervical lymphadenopathy, and splenomegaly. Atypical lymphocytosis and transaminase elevations are common, and the diagnosis of IM is confirmed with laboratory findings of a positive heterophile antibody ("Monospot"), polymerase chain reaction, or antibodies specific to EBV. Individuals with acute IM may be quite symptomatic and not feel well enough to participate in sports. Splenic enlargement is common, with rupture a relatively rare occurrence, typically occurring within a month of symptom onset, but this risk complicates sports participation, and is often the reason for restricting activity. The management of IM is primarily supportive, with no role for antivirals or corticosteroids. The variability of clinical presentation and the risk of splenic rupture in patients with IM present clinicians with challenging return to play/return to sport (RTS) decisions. This position statement updates the Evidence-Based Subject Review on Mononucleosis by the American Medical Society for Sports Medicine published in 2008 and reviews the epidemiology, clinical manifestations, laboratory assessment, and management including RTS for the athlete with IM. This statement also addresses complications, imaging, special considerations, diversity and equity considerations, and areas for future clinical research. Understanding the evidence regarding IM and sport is essential when communicating with athletes and their families and incorporating shared decision-making in the RTS decision.
Language	English
Publishing date	2023-05-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	1062530-6
ISSN	1536-3724 ; 1050-642X
ISSN (online)	1536-3724
ISSN	1050-642X
DOI	10.1097/JSM.0000000000001161
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Article ; Online: A circadian-like gene network programs the timing and dosage of heterochronic miRNA transcription during C. elegans development.

Kinney, Brian / Sahu, Shubham / Stec, Natalia / Hills-Muckey, Kelly / Adams, Dexter W / Wang, Jing / Jaremko, Matt / Joshua-Tor, Leemor / Keil, Wolfgang / Hammell, Christopher M

Developmental cell

2023 Volume 58, Issue 22, Page(s) 2563–2579.e8

Abstract: Development relies on the exquisite control of both the timing and the levels of gene expression to achieve robust developmental transitions. How cis- and trans-acting factors control both aspects simultaneously is unclear. We show that transcriptional ... ...

Abstract	Development relies on the exquisite control of both the timing and the levels of gene expression to achieve robust developmental transitions. How cis- and trans-acting factors control both aspects simultaneously is unclear. We show that transcriptional pulses of the temporal patterning microRNA (miRNA) lin-4 are generated by two nuclear hormone receptors (NHRs) in C. elegans, NHR-85 and NHR-23, whose mammalian orthologs, Rev-Erb and ROR, function in the circadian clock. Although Rev-Erb and ROR antagonize each other to control once-daily transcription in mammals, NHR-85/NHR-23 heterodimers bind cooperatively to lin-4 regulatory elements to induce a single pulse of expression during each larval stage. Each pulse's timing, amplitude, and duration are dictated by the phased expression of these NHRs and the C. elegans Period ortholog, LIN-42, that binds to and represses NHR-85. Therefore, during nematode temporal patterning, an evolutionary rewiring of circadian clock components couples the timing of gene expression to the control of transcriptional dosage.
MeSH term(s)	Animals ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Gene Regulatory Networks ; Gene Expression Regulation, Developmental ; Receptors, Cytoplasmic and Nuclear/metabolism ; Mammals/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	Caenorhabditis elegans Proteins ; MicroRNAs ; Receptors, Cytoplasmic and Nuclear ; LIN-42 protein, C elegans ; Transcription Factors
Language	English
Publishing date	2023-08-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2054967-2
ISSN	1878-1551 ; 1534-5807
ISSN (online)	1878-1551
ISSN	1534-5807
DOI	10.1016/j.devcel.2023.08.006
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Zs.A 5742: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 76: Show issues

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Article ; Online: The microRNA-argonaute complex: a platform for mRNA modulation.

Hammell, Christopher M

RNA biology

2008 Volume 5, Issue 3, Page(s) 123–127

Abstract: With the cloning the lin-4 gene in 1993, the possibility of an approximately 21-nucleotide RNA functioning as a regulatory molecule intrigued a relatively small number of scientists. This idea appeared to be a peculiarity of C. elegans as it was not ... ...

Abstract	With the cloning the lin-4 gene in 1993, the possibility of an approximately 21-nucleotide RNA functioning as a regulatory molecule intrigued a relatively small number of scientists. This idea appeared to be a peculiarity of C. elegans as it was not until seven years later that the second, more conserved small RNA, let-7 was cloned. A spate of papers in 2000 and 2001 revealed that the underlying properties of the lin-4 and let-7 genes were a common facet of animal genomes and the absolute number and potential of this new class of gene products requires us to integrate them with other aspects of gene expression and evolution.(1-3) A wealth of information has accumulated in the intervening years that outline, in general, how these small RNAs are expressed and processed into a functional form. Contemporaneous to these studies, experiments also identified a cadre of evolutionarily conserved proteins, the Argonautes (Agos) that directly associate with and are required for microRNA function. Computational and experimental methods have led the identification of many functional mRNA targets. In the last few years, a significant body of work has focused on resolving two key issues: How do microRNAs function in particular genetic contexts (i.e., as "molecular switches" or "fine-tuners" of gene expression) and secondly, what facet/s of mRNA metabolism do microRNAs modulate in their role(s) as a regulatory molecule? The primary objective here is not to comprehensively compare the competing models of microRNA function (reviewed in refs. 4-6) but to frame a potential solution to these two fundamental questions by suggesting that the core microRNA-Ribonucleic-Protein Complex (microRNP), composed of the microRNA and an Ago protein, functions as a highly modifiable scaffold that associates with specific mRNAs via the bound microRNA and facilitates the localized activity of a variety of accessory proteins. The resulting composite mechanism could account for the apparent complexities of measuring microRNA activity and furthermore, accommodate the broad levels of regulation observed in vivo.
MeSH term(s)	Animals ; Eukaryotic Initiation Factors/metabolism ; Humans ; MicroRNAs/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
Chemical Substances	Eukaryotic Initiation Factors ; MicroRNAs ; RNA, Messenger
Language	English
Publishing date	2008-07-08
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1555-8584
ISSN (online)	1555-8584
DOI	10.4161/rna.5.3.6570
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Article ; Online: Inducing RNAi in C. elegans by feeding with dsRNA-expressing E. coli.

Hammell, Christopher M / Hannon, Gregory J

Cold Spring Harbor protocols

2012 Volume 2012, Issue 12

Abstract: Owing to the relative ease and high-throughput nature of ingestion-mediated RNAi, the feeding of engineered Escherichia coli to wild-type and mutant Caenorhabditis elegans has developed into the most productive and common method to probe the functions of ...

Abstract	Owing to the relative ease and high-throughput nature of ingestion-mediated RNAi, the feeding of engineered Escherichia coli to wild-type and mutant Caenorhabditis elegans has developed into the most productive and common method to probe the functions of C. elegans genes. This protocol includes two variations of RNAi by feeding: one in which L1 larvae are fed dsRNA-expressing E. coli in liquid culture (commonly used to score effects of RNAi on postembryonic larval development) and another involving feeding of late larval- or adult-staged animals on standard solid medium culture plates.
MeSH term(s)	Animals ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/microbiology ; Caenorhabditis elegans/physiology ; Diet/methods ; Escherichia coli/genetics ; Gene Knockdown Techniques/methods ; Gene Silencing ; Larva/genetics ; Larva/microbiology ; Larva/physiology
Language	English
Publishing date	2012-12-01
Publishing country	United States
Document type	Journal Article
ISSN	1559-6095
ISSN (online)	1559-6095
DOI	10.1101/pdb.prot072348
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Article ; Online: An Epigenetic Priming Mechanism Mediated by Nutrient Sensing Regulates Transcriptional Output during C. elegans Development.

Stec, Natalia / Doerfel, Katja / Hills-Muckey, Kelly / Ettorre, Victoria M / Ercan, Sevinc / Keil, Wolfgang / Hammell, Christopher M

Current biology : CB

2020 Volume 31, Issue 4, Page(s) 809–826.e6

Abstract: Although precise tuning of gene expression levels is critical for most developmental pathways, the mechanisms by which the transcriptional output of dosage-sensitive molecules is established or modulated by the environment remain poorly understood. Here, ...

Abstract	Although precise tuning of gene expression levels is critical for most developmental pathways, the mechanisms by which the transcriptional output of dosage-sensitive molecules is established or modulated by the environment remain poorly understood. Here, we provide a mechanistic framework for how the conserved transcription factor BLMP-1/Blimp1 operates as a pioneer factor to decompact chromatin near its target loci during embryogenesis (hours prior to major transcriptional activation) and, by doing so, regulates both the duration and amplitude of subsequent target gene transcription during post-embryonic development. This priming mechanism is genetically separable from the mechanisms that establish the timing of transcriptional induction and functions to canalize aspects of cell-fate specification, animal size regulation, and molting. A key feature of the BLMP-1-dependent transcriptional priming mechanism is that chromatin decompaction is initially established during embryogenesis and maintained throughout larval development by nutrient sensing. This anticipatory mechanism integrates transcriptional output with environmental conditions and is essential for resuming normal temporal patterning after animals exit nutrient-mediated developmental arrests.
MeSH term(s)	Animals ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Chromatin ; Epigenesis, Genetic ; Gene Expression Regulation, Developmental ; Nutrients ; Transcription Factors/metabolism
Chemical Substances	Caenorhabditis elegans Proteins ; Chromatin ; Transcription Factors
Language	English
Publishing date	2020-12-22
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1071731-6
ISSN	1879-0445 ; 0960-9822
ISSN (online)	1879-0445
ISSN	0960-9822
DOI	10.1016/j.cub.2020.11.060
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Zs.A 3208: Show issues

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Article ; Online: LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification.

Science advances

2023 Volume 9, Issue 44, Page(s) eadh9543

Abstract: The genetic mechanisms underlying the expansion in size and complexity of the human brain remain poorly understood. Long interspersed nuclear element-1 (L1) retrotransposons are a source of divergent genetic information in hominoid genomes, but their ... ...

Abstract	The genetic mechanisms underlying the expansion in size and complexity of the human brain remain poorly understood. Long interspersed nuclear element-1 (L1) retrotransposons are a source of divergent genetic information in hominoid genomes, but their importance in physiological functions and their contribution to human brain evolution are largely unknown. Using multiomics profiling, we here demonstrate that L1 promoters are dynamically active in the developing and the adult human brain. L1s generate hundreds of developmentally regulated and cell type-specific transcripts, many that are co-opted as chimeric transcripts or regulatory RNAs. One L1-derived long noncoding RNA,
MeSH term(s)	Animals ; Humans ; Retroelements/genetics ; Transcriptome ; Long Interspersed Nucleotide Elements/genetics ; Neurons ; Primates/genetics
Chemical Substances	Retroelements
Language	English
Publishing date	2023-11-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2810933-8
ISSN	2375-2548 ; 2375-2548
ISSN (online)	2375-2548
ISSN	2375-2548
DOI	10.1126/sciadv.adh9543
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Article ; Online: Cell death machinery makes life more robust.

Aguirre-Chen, Cristina / Hammell, Christopher M

eLife

2014 Volume 3, Page(s) e05816

Abstract: CED-3, a protein that is essential for programmed cell death, also has an unexpected role in the regulation of non-apoptotic genes during normal development. ...

Abstract	CED-3, a protein that is essential for programmed cell death, also has an unexpected role in the regulation of non-apoptotic genes during normal development.
MeSH term(s)	Animals ; Apoptosis/genetics ; Caenorhabditis elegans/embryology ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans Proteins/metabolism ; Caspases/metabolism ; Embryonic Development/genetics ; Gene Expression Regulation, Developmental ; Humans ; MicroRNAs/metabolism
Chemical Substances	Caenorhabditis elegans Proteins ; MicroRNAs ; Caspases (EC 3.4.22.-)
Language	English
Publishing date	2014
Publishing country	England
Document type	Comment ; Journal Article
ISSN	2050-084X
ISSN (online)	2050-084X
DOI	10.7554/eLife.05816
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