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Article ; Online: A semi‑automated FISH‑based micronucleus‑centromere assay for biomonitoring of hospital workers exposed to low doses of ionizing radiation.

Vral, Anne / Decorte, Veerle / Depuydt, Julie / Wambersie, André / Thierens, Hubert

2016 Volume 14, Issue 1, Page(s) 103–110

Abstract: The aim of the present study was to perform cytogenetic analysis by means of a semi‑automated micronucleus‑centromere assay in lymphocytes from medical radiation workers. Two groups of workers receiving the highest occupational doses were selected: 10 ... ...

Abstract	The aim of the present study was to perform cytogenetic analysis by means of a semi‑automated micronucleus‑centromere assay in lymphocytes from medical radiation workers. Two groups of workers receiving the highest occupational doses were selected: 10 nuclear medicine technicians and 10 interventional radiologists/cardiologists. Centromere‑negative micronucleus (MNCM‑) data, obtained from these two groups of medical radiation workers were compared with those obtained in matched controls. The blood samples of the matched controls were additionally used to construct a 'low‑dose' (0‑100 mGy) MNCM‑ dose‑response curve to evaluate the sensitivity and suitability of the micronucleus‑centromere assay as an 'effect' biomarker in medical surveillance programs. The physical dosimetry data of the 3 years preceding the blood sampling, based on single or double dosimetry practices, were collected for the interpretation of the micronucleus data. The in vitro radiation results showed that for small sized groups, semi‑automated scoring of MNCM‑ enables the detection of a dose of 50 mGy. The comparison of MNCM‑ yields in medical radiation workers and control individuals showed enhanced MNCM‑ scores in the medical radiation workers group (P=0.15). The highest MNCM‑ scores were obtained in the interventional radiologists/cardiologists group, and these scores were significantly higher compared with those obtained from the matched control group (P=0.05). The higher MNCM‑ scores observed in interventional radiologists/cardiologists compared with nuclear medicine technicians were not in agreement with the personal dosimetry records in both groups, which may point to the limitation of 'double dosimetry' procedures used in interventional radiology/cardiology. In conclusion, the data obtained in the present study supports the importance of cytogenetic analysis, in addition to physical dosimetry, as a routine biomonitoring method in medical radiation workers receiving the highest occupational radiation burdens.
MeSH term(s)	Case-Control Studies ; Centromere/genetics ; Dose-Response Relationship, Radiation ; Environmental Monitoring ; Humans ; In Situ Hybridization, Fluorescence/methods ; Micronucleus Tests/methods ; Occupational Exposure ; Personnel, Hospital ; Radiation Dosage ; Radiation, Ionizing ; Radiometry
Language	English
Publishing date	2016-05-13
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2469505-1
ISSN	1791-3004 ; 1791-2997
ISSN (online)	1791-3004
ISSN	1791-2997
DOI	10.3892/mmr.2016.5265
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Article ; Online: Tumour characteristics of screen-detected and interval cancers in the Flemish Breast Cancer Screening Programme: A mammographic breast density study.

Timmermans, Lore / De Brabander, Isabel / Van Damme, Nancy / Bleyen, Luc / Martens, Patrick / Van Herck, Koen / Thierens, Hubert / Bacher, Klaus / Depypere, Herman

Maturitas

2021 Volume 158, Page(s) 55–60

Abstract: Objective: The objective is to investigate tumour prognostic factors versus breast density in screen-detected cancers and interval cancers. The results may highlight the need for more personalised screening protocols based on breast density in organized ...

Abstract	Objective: The objective is to investigate tumour prognostic factors versus breast density in screen-detected cancers and interval cancers. The results may highlight the need for more personalised screening protocols based on breast density in organized screening programmes. Study design: A retrospective study was performed of tumour characteristics of screen-detected cancers (n=468) and interval cancers (n=515) of 983 women who participated in the Flemish Breast Cancer Screening Programme in 2009-2010. Breast density was obtained from the screening programme data. Information on nodal invasion and histological grading was taken from the Belgian Cancer Registry. Tumour size and proliferation and receptor expression status were retrieved from pathology reports. The differences in tumour characteristics between screen-detected and interval cancers as well as the variation in these variables with breast density in both groups were studied by logistic regression. Results: A comparison of tumour characteristics between screen-detected cancers and interval cancers systematically showed features of more aggressive tumours in interval cancers: larger tumour size, nodal invasion, grade 3 tumours, and hormone receptor negative phenotype (p<0.05). The analysis of tumour characteristics versus breast density in screen-detected cancers showed higher numbers of aggressive grade 3 tumours in low-density breasts and of the luminal A subtype with good prognosis in high-density breasts (p<0.05). This analysis for interval cancers highlights a high proportion of the difficult-to-treat triple-negative subtype in low-density breasts compared with high-density breasts. In conclusion, the study data support arguments against changes in breast cancer screening programmes with prolongation of screening intervals in low-density breasts.
MeSH term(s)	Breast Density ; Breast Neoplasms/diagnostic imaging ; Breast Neoplasms/pathology ; Early Detection of Cancer ; Female ; Humans ; Mammography/methods ; Mass Screening/methods ; Retrospective Studies
Language	English
Publishing date	2021-12-17
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	80460-5
ISSN	1873-4111 ; 0378-5122
ISSN (online)	1873-4111
ISSN	0378-5122
DOI	10.1016/j.maturitas.2021.12.006
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Zs.A 1432: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: DNA double strand breaks induced by low dose mammography X-rays in breast tissue: A pilot study.

Depuydt, Julie / Viaene, Tanguy / Blondeel, Phillip / Roche, Nathalie / Van den Broecke, Rudy / Thierens, Hubert / Vral, Anne

Oncology letters

2018 Volume 16, Issue 3, Page(s) 3394–3400

Abstract: Breast tissue is very sensitive to ionizing radiation due to the presence of reproductive hormones, including estrogen. In the present pilot study, the efficiency of mammography X-rays to induce DNA double strand breaks (DSB) in mammary epithelial cells ... ...

Abstract	Breast tissue is very sensitive to ionizing radiation due to the presence of reproductive hormones, including estrogen. In the present pilot study, the efficiency of mammography X-rays to induce DNA double strand breaks (DSB) in mammary epithelial cells was investigated. For this, freshly resected healthy breast tissue was irradiated with 30 kV mammography X-rays in the dose range 0-500 mGy (2, 4, 10, 20, 40, 100 and 500 mGy). Breast specimens were also irradiated with identical doses of
Language	English
Publishing date	2018-06-26
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2573196-8
ISSN	1792-1082 ; 1792-1074
ISSN (online)	1792-1082
ISSN	1792-1074
DOI	10.3892/ol.2018.9024
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Article: The micronucleus assay in radiation accidents.

Thierens, Hubert / Vral, Anne

Annali dell'Istituto superiore di sanita

2009 Volume 45, Issue 3, Page(s) 260–264

Abstract: The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is a standardised and validated technique for biodosimetry. Automated scoring of micronuclei allows large scale applications as in population triage in case of radiation accidents ... ...

Abstract	The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is a standardised and validated technique for biodosimetry. Automated scoring of micronuclei allows large scale applications as in population triage in case of radiation accidents or malevolent use of radioactive sources. The dose detection limit (95% confidence) of the micronucleus assay for individual dose assessment is restricted to 0.2 Gy but can be decreased to 0.1 Gy by scoring centromeres in micronuclei using fluorescence in situ hybridization (FISH). In the past the micronucleus assay was applied for a number of large scale biomonitoring studies of nuclear power plant workers and hospital workers. Baseline micronucleus frequencies depend strongly on age and gender. The assay was also already used for biodosimetry of radiation accidents. In a multiple endpoint biodosimetry study for dose assessment of a worker exposed accidentally in 2003 to X-rays, a good agreement was obtained between dose estimates resulting from the micronucleus assay, the scoring of dicentrics and translocations. Automated scoring of micronuclei in combination with centromere signals, allowing systematic biodosimetry of exposed populations, remains a challenge for the future.
MeSH term(s)	Automation ; Centromere/radiation effects ; Centromere/ultrastructure ; Humans ; Lymphocytes/radiation effects ; Micronucleus Tests/methods ; Radioactive Hazard Release ; Radiometry/methods
Language	English
Publishing date	2009
Publishing country	Italy
Document type	Journal Article ; Review
ZDB-ID	950344-4
ISSN	0021-2571
ISSN	0021-2571
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Zs.A 231: Show issues

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Article ; Online: The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure.

Vral, Anne / Fenech, Michael / Thierens, Hubert

Mutagenesis

2011 Volume 26, Issue 1, Page(s) 11–17

Abstract: Biological dosimetry, based on the analysis of micronuclei (MN) in the cytokinesis-block micronucleus (CBMN) assay can be used as an alternative method for scoring dicentric chromosomes in the field of radiation protection. Biological dosimetry or ... ...

Abstract	Biological dosimetry, based on the analysis of micronuclei (MN) in the cytokinesis-block micronucleus (CBMN) assay can be used as an alternative method for scoring dicentric chromosomes in the field of radiation protection. Biological dosimetry or Biodosimetry, is mainly performed, in addition to physical dosimetry, with the aim of individual dose assessment. Many studies have shown that the number of radiation-induced MN is strongly correlated with dose and quality of radiation. The CBMN assay has become, in the last years, a thoroughly validated and standardised technique to evaluate in vivo radiation exposure of occupational, medical and accidentally exposed individuals. Compared to the gold standard, the dicentric assay, the CBMN assay has the important advantage of allowing economical, easy and quick analysis. The main disadvantage of the CBMN assay is related to the variable micronucleus (MN) background frequency, by which only in vivo exposures in excess of 0.2-0.3 Gy X-rays can be detected. In the last years, several improvements have been achieved, with the ultimate goals (i) of further increasing the sensitivity of the CBMN assay for low-dose detection by combining the assay with a fluorescence in situ hybridisation centromere staining technique, (ii) of increasing the specificity of the test for radiation by scoring nucleoplasmic bridges in binucleated cells and (iii) of making the assay optimally suitable for rapid automated analysis of a large number of samples, viz. in case of a large-scale radiation accident. The development of a combined automated MN-centromere scoring procedure remains a challenge for the future, as it will allow systematic biomonitoring of radiation workers exposed to low-dose radiation.
MeSH term(s)	Dose-Response Relationship, Radiation ; Humans ; Lymphocytes/radiation effects ; Micronuclei, Chromosome-Defective ; Micronucleus Tests ; Radiation, Ionizing
Language	English
Publishing date	2011-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	632903-2
ISSN	1464-3804 ; 0267-8357
ISSN (online)	1464-3804
ISSN	0267-8357
DOI	10.1093/mutage/geq078
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Zs.A 2189: Show issues

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Article ; Online: Virtual electron microscopy in cell biology.

Mione, Sylvia / Bacher, Klaus / Thierens, Hubert / Cornelissen, Maria

Microscopy research and technique

2011 Volume 74, Issue 3, Page(s) 251–256

Abstract: Virtual microscopy of histological glass slides can emulate conventional light microscopy. Up till now, such a digital simulation does not exist for ultrathin electron microscopic slides. Because of the relative inaccessibility of electron microscopy, ... ...

Abstract	Virtual microscopy of histological glass slides can emulate conventional light microscopy. Up till now, such a digital simulation does not exist for ultrathin electron microscopic slides. Because of the relative inaccessibility of electron microscopy, evaluation of subcellular structures by (bio)medical students is performed with the aid of photographic prints. In this article, the generation and evaluation of virtual electron microscopic slides is discussed. A T-lymphoblastic cell was used as an example. Electron microscopic pictures were taken at two magnifications (25,000 and 50,000), processed in an analogue or digital way and stitched to reconstruct the image of the total cell. This image is viewed with a webviewer equipped with pan and zoom functions. The possibility of distinguishing the trilaminar structure of cellular membranes was the requisite. Virtual images obtained at an original magnification of 25,000, scanned at a resolution of 800 ppi could compete with pictures developed directly from negatives obtained by electron microscopy. It is possible to navigate and zoom into details in a way emulating electron microscopy. Virtual electron microscopy is innovative and offers new perspectives to interpret cytological pictures and to teach cell biology in an interactive and unique way.
MeSH term(s)	Cell Biology/instrumentation ; Cell Line, Tumor ; Humans ; Image Processing, Computer-Assisted/methods ; Microscopy, Electron, Transmission/methods ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; T-Lymphocytes/cytology
Language	English
Publishing date	2011-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	1099714-3
ISSN	1097-0029 ; 1059-910X
ISSN (online)	1097-0029
ISSN	1059-910X
DOI	10.1002/jemt.20898
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Zs.A 2251: Show issues

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Article ; Online: Relative biological effectiveness of mammography X-rays at the level of DNA and chromosomes in lymphocytes.

Depuydt, Julie / Baert, Annelot / Vandersickel, Veerle / Thierens, Hubert / Vral, Anne

International journal of radiation biology

2013 Volume 89, Issue 7, Page(s) 532–538

Abstract: Purpose: In many countries, breast cancer screening programs based on periodic mammography exist, giving a large group of women regularly a small dose of ionizing radiation. In order to assess the benefit/risk ratio of those programs the relative ... ...

Abstract	Purpose: In many countries, breast cancer screening programs based on periodic mammography exist, giving a large group of women regularly a small dose of ionizing radiation. In order to assess the benefit/risk ratio of those programs the relative biological effectiveness (RBE) of mammography X-rays needs to be determined. Materials and methods: Blood of five healthy donors was irradiated in vitro with 30 kV X-rays and (60)Co γ-rays with doses between 5 and 2000 mGy. The phosphorylated histone subtype H2A isoform X-foci (γH2AX-foci) technique was used to quantify the number of DNA double-strand breaks (DSB) after irradiation. Chromosomal damage resulting from non- or misrepaired DNA DSB was quantified with the micronucleus (MN)-assay and the sensitivity was improved by counting only centromere negative micronuclei (MNCM-). Results: The threshold detection dose obtained with the γH2AX-foci test was 10 mGy for mammography X-rays compared to 50 mGy for γ-rays. With the MN-assay respectively MN-centromere-assay threshold detection doses of 100, respectively, 50 mGy were obtained for mammography X-rays compared to 200 respectively 100 mGy for γ-rays. An RBE of 1.4 was obtained with the γH2AX-foci assay. With the MN-assays low-dose RBE values between 3 and 4 were determined. Conclusion: Our results indicate that exposure to mammography X-rays resulted in a modest increase in the induction of DSB compared to γ-rays. However, due to the higher linear energy transfer (LET) of mammography X-rays more clustered DNA damage is produced that is more difficult to repair and results in a more pronounced increase in micronucleus formation.
MeSH term(s)	Cells, Cultured ; Chromosome Breakage/radiation effects ; DNA/genetics ; DNA/radiation effects ; DNA Damage/physiology ; Dose-Response Relationship, Radiation ; Female ; Humans ; Leukocytes, Mononuclear/physiology ; Leukocytes, Mononuclear/radiation effects ; Mammography ; Radiation Dosage ; Relative Biological Effectiveness ; X-Rays
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2013-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3065-x
ISSN	1362-3095 ; 0020-7616 ; 0955-3002
ISSN (online)	1362-3095
ISSN	0020-7616 ; 0955-3002
DOI	10.3109/09553002.2013.782447
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Article ; Online: Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes.

El-Saghire, Houssein / Michaux, Arlette / Thierens, Hubert / Baatout, Sarah

International journal of molecular medicine

2013 Volume 32, Issue 6, Page(s) 1407–1414

Abstract: The health effects arising from exposure to low doses of ionizing radiation are of particular concern, mainly due to the increased application of diagnostic and therapeutic X-ray modalities. The mechanisms behind the cell and tissue responses to low ... ...

Abstract	The health effects arising from exposure to low doses of ionizing radiation are of particular concern, mainly due to the increased application of diagnostic and therapeutic X-ray modalities. The mechanisms behind the cell and tissue responses to low doses remain to be elucidated. Accumulating evidence suggests that low doses of ionizing radiation induce activation of the immune response; however, the processes involved have yet to be adequately investigated. Monocytes are key players in the induction of an immune response. Within the context of this study, we investigated the activation of toll-like receptors (TLRs), mitogen‑activated protein kinases (MAPKs) and NF-κB signaling in isolated human primary monocytes in response to low doses (0.05 and 0.1 Gy) and a high dose (1 Gy) of ionizing radiation. Using quantitative RT-PCR and ELISA techniques, our results showed a positive regulation of TLR signaling in response to low doses but a less significant response at high doses. This activation was demonstrated via the activation of TLR signaling molecules (HMGB1, TLR4, TLR9, MyD88 and IRAK1). Furthermore, and in contrast to the high dose, the low doses showed increased phosphorylation levels of the protein IκBα, and therefore positive signaling of the NF-κB pathway. This result denotes pro-survival and pro-inflammatory responses. Additionally, MAPKs were activated in response to 0.05 Gy, while 0.1 and 1 Gy showed a downregulatory trend that may be related to activation of the PF4 gene. On the other hand, there was highly significant involvement of activated p53 and damaged genes in response to high but not low doses. In conclusion, this study addressed the need to re-evaluate health risks arising from exposure to low doses of ionizing radiation, particularly in view of the accumulating evidence reporting inflammatory and oncogenic consequences from these exposures.
MeSH term(s)	Apoptosis/radiation effects ; Cell Cycle Checkpoints/radiation effects ; Cell Separation ; Cell Survival/radiation effects ; Cells, Cultured ; DNA Damage ; Dose-Response Relationship, Radiation ; Down-Regulation/radiation effects ; Enzyme Activation/radiation effects ; HMGB1 Protein/metabolism ; Humans ; Mitogen-Activated Protein Kinases/metabolism ; Models, Biological ; Monocytes/immunology ; Monocytes/radiation effects ; NF-kappa B/metabolism ; Phosphorylation/radiation effects ; Platelet Factor 4/genetics ; Platelet Factor 4/metabolism ; Radiation, Ionizing ; Signal Transduction/radiation effects ; Toll-Like Receptors/metabolism ; Tumor Suppressor Protein p53/metabolism
Chemical Substances	HMGB1 Protein ; NF-kappa B ; Toll-Like Receptors ; Tumor Suppressor Protein p53 ; Platelet Factor 4 (37270-94-3) ; Mitogen-Activated Protein Kinases (EC 2.7.11.24)
Language	English
Publishing date	2013-12
Publishing country	Greece
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1444428-8
ISSN	1791-244X ; 1107-3756
ISSN (online)	1791-244X
ISSN	1107-3756
DOI	10.3892/ijmm.2013.1514
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Zs.A 4942: Show issues

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Article ; Online: Dose response and repair kinetics of gamma-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and gamma-radiation.

Beels, Laurence / Werbrouck, Joke / Thierens, Hubert

International journal of radiation biology

2010 Volume 86, Issue 9, Page(s) 760–768

Abstract: Purpose: Dose response and repair kinetics of phosphorylated histone H2A isoform X (gamma-H2AX) foci in T-lymphocytes were investigated in the low-dose range after in vitro irradiation of whole blood and T-lymphocytes with 100 kVp X-rays and (60)Co ... ...

Abstract	Purpose: Dose response and repair kinetics of phosphorylated histone H2A isoform X (gamma-H2AX) foci in T-lymphocytes were investigated in the low-dose range after in vitro irradiation of whole blood and T-lymphocytes with 100 kVp X-rays and (60)Co gamma-rays. Materials and methods: Whole blood or isolated T-lymphocytes were irradiated in vitro and gamma-H2AX foci were scored. Dose response was determined in the 0-500 mGy dose range. Foci kinetics were studied at doses of 5 and 200 mGy up to 24 h post-irradiation. Results: After X-irradiation, the dose response for whole blood shows a biphasic behaviour with a low-dose hypersensitivity, which is less pronounced for isolated T-lymphocytes. In contrast, gamma-radiation shows a linear dose response for both irradiation conditions. Concerning repair kinetics, delayed repair was found after X-ray whole blood irradiation (5 and 200 mGy) with 40% of the foci persisting 24 h post-irradiation. This number of foci is reduced to 10% after irradiation of isolated T-lymphocytes with 200 mGy X-rays. On the contrary, gamma-H2AX foci are reduced to background levels 24 h post-irradiation with 200 mGy (60)Co gamma-rays. Conclusion: gamma-H2AX foci response and repair kinetics depend on irradiation conditions and radiation quality, possibly linked to Bystander response.
MeSH term(s)	Adult ; Blood Cells/chemistry ; Blood Cells/radiation effects ; DNA Damage ; DNA Repair ; Dose-Response Relationship, Radiation ; Female ; Gamma Rays ; Histones/analysis ; Humans ; Male ; T-Lymphocytes/chemistry ; T-Lymphocytes/radiation effects ; X-Rays ; Young Adult
Chemical Substances	H2AX protein, human ; Histones
Language	English
Publishing date	2010-07-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3065-x
ISSN	1362-3095 ; 0020-7616 ; 0955-3002
ISSN (online)	1362-3095
ISSN	0020-7616 ; 0955-3002
DOI	10.3109/09553002.2010.484479
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Article ; Online: An optimized growth factor cocktail for ovine mesenchymal stem cells.

Somers, Pamela / Cornelissen, Ria / Thierens, Hubert / Van Nooten, Guido

Growth factors (Chur, Switzerland)

2012 Volume 30, Issue 1, Page(s) 37–48

Abstract: Growth factors that regulate proliferation, migration, and invasion of ovine mesenchymal stem cells (oMSCs) are not well defined. In this study, we have evaluated five growth factors for their ability to initiate and support in vitro proliferation, ... ...

Abstract	Growth factors that regulate proliferation, migration, and invasion of ovine mesenchymal stem cells (oMSCs) are not well defined. In this study, we have evaluated five growth factors for their ability to initiate and support in vitro proliferation, migration, and invasion of oMSCs. oMSCs were exposed to different doses and combinations of the growth factors: basic fibroblast growth factor (bFGF), transforming growth factor-β (TGF-β), epidermal growth factor (EGF), insulin growth factor-I (IGF-I), connective tissue growth factor, and platelet-derived growth factor-AB (PDGF-AB). Cellular proliferation, motility, and invasiveness were assayed. The most proliferative stimulating growth factors are PDGF-AB+TGF-β and PDGF-AB+IGF-I. Combinations EGF+bFGF and EGF+bFGF+PDGF-AB demonstrated the greatest ability to stimulate migration. Moreover, the triple cocktail EGF+bFGF+TGF-β has the most significant effect on invasion. Different growth factor cocktails are required to enhance proliferation, migration, and invasion. These results may be useful for the development of a tissue-engineered heart valve by stimulating cellular repopulation.
MeSH term(s)	Animals ; Cell Differentiation ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Epidermal Growth Factor/metabolism ; Epidermal Growth Factor/pharmacology ; Female ; Fibroblast Growth Factor 2/metabolism ; Fibroblast Growth Factor 2/pharmacology ; Heart Valves ; Humans ; Intercellular Signaling Peptides and Proteins/metabolism ; Intercellular Signaling Peptides and Proteins/pharmacology ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/drug effects ; Mesenchymal Stromal Cells/physiology ; Sheep, Domestic ; Tissue Engineering/methods ; Transforming Growth Factor beta/metabolism ; Transforming Growth Factor beta/pharmacology
Chemical Substances	Intercellular Signaling Peptides and Proteins ; Transforming Growth Factor beta ; Fibroblast Growth Factor 2 (103107-01-3) ; Epidermal Growth Factor (62229-50-9)
Language	English
Publishing date	2012-02
Publishing country	England
Document type	Evaluation Studies ; Journal Article
ZDB-ID	1035755-5
ISSN	1029-2292 ; 0897-7194
ISSN (online)	1029-2292
ISSN	0897-7194
DOI	10.3109/08977194.2011.634411
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