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Article: Directive clinique n

Fung-Kee-Fung, Karen / Wong, Karen / Walsh, Jennifer / Hamel, Candyce / Clarke, Gwen

Journal of obstetrics and gynaecology Canada : JOGC = Journal d'obstetrique et gynecologie du Canada : JOGC

2024 , Page(s) 102448

Language	English
Publishing date	2024-03-28
Publishing country	Netherlands
Document type	Practice Guideline
ZDB-ID	2171082-X
ISSN	1701-2163
ISSN	1701-2163
DOI	10.1016/j.jogc.2024.102448
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Article ; Online: N-acetylcysteine: a novel approach to methaemoglobinaemia in normothermic liver machine perfusion.

Clarke, George / Mao, Jingwen / Fan, Yiyu / Hann, Angus / Gupta, Amita / Nutu, Anisa / Buckel, Erwin / Kayani, Kayani / Murphy, Nicholas / Bangash, Mansoor N / Casey, Anna L / Wootton, Isla / Lawson, Alexander J / Dasari, Bobby V M / Perera, M Thamara P R / Mergental, Hynek / Afford, Simon C

Scientific reports

2023 Volume 13, Issue 1, Page(s) 19022

Abstract: ... N-acetylcysteine (NAC) on the accumulation of methaemoglobin. NMP was performed on nine human donor ...

Abstract	Extended duration of normothermic machine perfusion (NMP) provides opportunities to resuscitate suboptimal donor livers. This intervention requires adequate oxygen delivery typically provided by a blood-based perfusion solution. Methaemoglobin (MetHb) results from the oxidation of iron within haemoglobin and represents a serious problem in perfusions lasting > 24 h. We explored the effects of anti-oxidant, N-acetylcysteine (NAC) on the accumulation of methaemoglobin. NMP was performed on nine human donor livers declined for transplantation: three were perfused without NAC (no-NAC group), and six organs perfused with an initial NAC bolus, followed by continuous infusion (NAC group), with hourly methaemoglobin perfusate measurements. In-vitro experiments examined the impact of NAC (3 mg) on red cells (30 ml) in the absence of liver tissue. The no-NAC group sustained perfusions for an average of 96 (range 87-102) h, universally developing methaemoglobinaemia (≥ 2%) observed after an average of 45 h, with subsequent steep rise. The NAC group was perfused for an average of 148 (range 90-184) h. Only 2 livers developed methaemoglobinaemia (peak MetHb of 6%), with an average onset of 116.5 h. Addition of NAC efficiently limits formation and accumulation of methaemoglobin during NMP, and allows the significant extension of perfusion duration.
MeSH term(s)	Humans ; Liver Transplantation/methods ; Methemoglobinemia ; Acetylcysteine/pharmacology ; Organ Preservation/methods ; Methemoglobin ; Liver ; Perfusion/methods
Chemical Substances	Acetylcysteine (WYQ7N0BPYC) ; Methemoglobin (9008-37-1)
Language	English
Publishing date	2023-11-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-45206-z
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Article ; Online: N-acetylcysteine

George Clarke / Jingwen Mao / Yiyu Fan / Angus Hann / Amita Gupta / Anisa Nutu / Erwin Buckel / Kayani Kayani / Nicholas Murphy / Mansoor N. Bangash / Anna L. Casey / Isla Wootton / Alexander J. Lawson / Bobby V. M. Dasari / M. Thamara P. R. Perera / Hynek Mergental / Simon C. Afford

Scientific Reports, Vol 13, Iss 1, Pp 1-

a novel approach to methaemoglobinaemia in normothermic liver machine perfusion

2023 Volume 8

Abstract: ... the effects of anti-oxidant, N-acetylcysteine (NAC) on the accumulation of methaemoglobin. NMP was performed on nine human ...

Abstract	Abstract Extended duration of normothermic machine perfusion (NMP) provides opportunities to resuscitate suboptimal donor livers. This intervention requires adequate oxygen delivery typically provided by a blood-based perfusion solution. Methaemoglobin (MetHb) results from the oxidation of iron within haemoglobin and represents a serious problem in perfusions lasting > 24 h. We explored the effects of anti-oxidant, N-acetylcysteine (NAC) on the accumulation of methaemoglobin. NMP was performed on nine human donor livers declined for transplantation: three were perfused without NAC (no-NAC group), and six organs perfused with an initial NAC bolus, followed by continuous infusion (NAC group), with hourly methaemoglobin perfusate measurements. In-vitro experiments examined the impact of NAC (3 mg) on red cells (30 ml) in the absence of liver tissue. The no-NAC group sustained perfusions for an average of 96 (range 87–102) h, universally developing methaemoglobinaemia (≥ 2%) observed after an average of 45 h, with subsequent steep rise. The NAC group was perfused for an average of 148 (range 90–184) h. Only 2 livers developed methaemoglobinaemia (peak MetHb of 6%), with an average onset of 116.5 h. Addition of NAC efficiently limits formation and accumulation of methaemoglobin during NMP, and allows the significant extension of perfusion duration.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
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Article ; Online: N-glycoproteomics of brain synapses and synaptic vesicles.

Bradberry, Mazdak M / Peters-Clarke, Trenton M / Shishkova, Evgenia / Chapman, Edwin R / Coon, Joshua J

Cell reports

2023 Volume 42, Issue 4, Page(s) 112368

Abstract: ... neurotransmission. Asparagine (N)-linked glycosylation is required for delivery of the major SV glycoproteins ... synaptophysin and SV2A to SVs. Despite this key role for N-glycosylation, the molecular compositions of SV N ... mass spectrometry to characterize N-glycosylation at synapses and SVs from mouse brain. Detecting over 2,500 unique ...

Abstract	At mammalian neuronal synapses, synaptic vesicle (SV) glycoproteins are essential for robust neurotransmission. Asparagine (N)-linked glycosylation is required for delivery of the major SV glycoproteins synaptophysin and SV2A to SVs. Despite this key role for N-glycosylation, the molecular compositions of SV N-glycans are largely unknown. In this study, we combined organelle isolation techniques and high-resolution mass spectrometry to characterize N-glycosylation at synapses and SVs from mouse brain. Detecting over 2,500 unique glycopeptides, we found that SVs harbor a distinct population of oligomannose and highly fucosylated N-glycans. Using complementary fluorescence methods, we identify at least one highly fucosylated N-glycan enriched in SVs compared with synaptosomes. High fucosylation was characteristic of SV proteins, plasma membrane proteins, and cell adhesion molecules with key roles in synaptic function and development. Our results define the N-glycoproteome of a specialized neuronal organelle and inform timely questions in the glycobiology of synaptic pruning and neuroinflammation.
MeSH term(s)	Mice ; Animals ; Synaptic Vesicles/metabolism ; Synapses/metabolism ; Glycoproteins/metabolism ; Brain/metabolism ; Polysaccharides/metabolism ; Mammals/metabolism
Chemical Substances	Glycoproteins ; Polysaccharides
Language	English
Publishing date	2023-04-09
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2023.112368
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Article ; Online: Involvement of the alpha-subunit N-terminus in the mechanism of the Na

Lev, B / Chennath, M / Cranfield, C G / Cornelius, F / Allen, T W / Clarke, R J

Biochimica et biophysica acta. Molecular cell research

2023 Volume 1870, Issue 7, Page(s) 119539

Abstract: Previous studies have shown that cytoplasmic ... ...

Abstract	Previous studies have shown that cytoplasmic K
MeSH term(s)	Animals ; Rats ; Sodium-Potassium-Exchanging ATPase/genetics ; Sodium-Potassium-Exchanging ATPase/metabolism ; Protein Conformation ; Cell Membrane/metabolism ; Molecular Dynamics Simulation ; Sodium/metabolism ; Lipids
Chemical Substances	Sodium-Potassium-Exchanging ATPase (EC 7.2.2.13) ; Sodium (9NEZ333N27) ; Lipids
Language	English
Publishing date	2023-07-20
Publishing country	Netherlands
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbamcr.2023.119539
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Article ; Online: Rational Design of a Facially Coordinating P,N,N Ligand for Manganese-Catalysed Enantioselective Hydrogenation of Cyclic Ketones.

Oates, Conor L / Goodfellow, Alister S / Bühl, Michael / Clarke, Matthew L

Angewandte Chemie (International ed. in English)

2022 Volume 62, Issue 3, Page(s) e202212479

Abstract: DFT calculations on the full catalytic cycle for manganese catalysed enantioselective hydrogenation of a selection of ketones have been carried out at the PBE0- ... ...

Abstract	DFT calculations on the full catalytic cycle for manganese catalysed enantioselective hydrogenation of a selection of ketones have been carried out at the PBE0-D3
Language	English
Publishing date	2022-12-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.202212479
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Article ; Online: Description of Culicoides truuskae sp. n. (Diptera: Ceratopogonidae) from southern Africa.

Labuschagne, Karien / Meiswinkel, Rudolf / Liebenberg, Danica / Van Zyl, Carissa / Van Schalkwyk, Antoinette / Scholtz, Clarke

The Onderstepoort journal of veterinary research

2023 Volume 90, Issue 1, Page(s) e1–e14

Abstract: Culicoides truuskae Labuschagne and Meiswinkel sp. n. is described and illustrated in both sexes ... Savanna ecoregions in Namibia experiencing 600 mm of rainfall annually. Culicoides truuskae sp. n. is part ... the diagnostic dark smudge that traverses wing cell r3 may result in C. truuskae sp. n. being misidentified ...

Abstract	Culicoides truuskae Labuschagne and Meiswinkel sp. n. is described and illustrated in both sexes from material collected in South Africa and Namibia. It is restricted to the xeric western margin of the subcontinent, occurring in Fynbos, Nama-Karoo and Succulent Karoo ecoregions in South Africa and Desert and Savanna ecoregions in Namibia experiencing 600 mm of rainfall annually. Culicoides truuskae sp. n. is part of the Afrotropical 'plain-wing' Culicoides in which the wing lacks a distinguishing pattern of light and dark spots; the diagnostic dark smudge that traverses wing cell r3 may result in C. truuskae sp. n. being misidentified as the sympatric but phyletically unrelated Culicoides herero (Enderlein) - (of the Similis group, subgenus Oecacta Poey). Additionally, this study is the first description of the male of C. herero. C. truuskae sp. n. and Culicoides coarctatus Clastrier and Wirth share similar characters in the male genitalia, although the two species are separable on wing pattern and female flagellum sensilla coeloconica (SCo) distribution. The breeding habitat and adult female blood-feeding preferences of C. truuskae sp. n. are not known. A maximum likelihood phylogenetic tree, using mitochondrial cytochrome c oxidase I (COI) sequence data, is provided to further clarify the relationship between C. truuskae sp. n., C. coarctatus and C. herero. Extensive light trap data, collected over 30 years, are used to map the distribution ranges of C. truuskae sp. n., C. coarctatus and C. herero in Southern Africa.Contribution: The description of this new species and the description of the male of C. herero increases our understanding of the diversity and distribution of Culicoides species in southern Africa.
MeSH term(s)	Female ; Male ; Animals ; Ceratopogonidae ; Phylogeny ; Africa, Southern ; South Africa ; Namibia
Language	English
Publishing date	2023-03-06
Publishing country	South Africa
Document type	Journal Article
ZDB-ID	417084-2
ISSN	2219-0635 ; 0030-2465
ISSN (online)	2219-0635
ISSN	0030-2465
DOI	10.4102/ojvr.v90i1.2072
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Article ; Online: The "humanized" N-glycosylation pathway in CRISPR/Cas9-edited Nicotiana benthamiana significantly enhances the immunogenicity of a S/preS1 Hepatitis B Virus antigen and the virus-neutralizing antibody response in vaccinated mice.

Pantazica, Ana-Maria / van Eerde, André / Dobrica, Mihaela-Olivia / Caras, Iuliana / Ionescu, Irina / Costache, Adriana / Tucureanu, Catalin / Steen, Hege / Lazar, Catalin / Heldal, Inger / Haugslien, Sissel / Onu, Adrian / Stavaru, Crina / Branza-Nichita, Norica / Liu Clarke, Jihong

Plant biotechnology journal

2023 Volume 21, Issue 6, Page(s) 1176–1190

Abstract: ... KO), by CRISPR/Cas9 genome editing, enables production of proteins with "humanized" N-glycosylation ... In this study, we investigated the impact of plant N-glycosylation on the immunogenic properties of a chimeric ...

Abstract	The recent SARS-CoV-2 pandemic has taught the world a costly lesson about the devastating consequences of viral disease outbreaks but also, the remarkable impact of vaccination in limiting life and economic losses. Vaccination against human Hepatitis B Virus (HBV), a major human pathogen affecting 290 million people worldwide, remains a key action towards viral hepatitis elimination by 2030. To meet this goal, the development of improved HBV antigens is critical to overcome non-responsiveness to standard vaccines based on the yeast-produced, small (S) envelope protein. We have recently shown that combining relevant immunogenic determinants of S and large (L) HBV proteins in chimeric antigens markedly enhances the anti-HBV immune response. However, the demand for cost-efficient, high-quality antigens remains challenging. This issue could be addressed by using plants as versatile and rapidly scalable protein production platforms. Moreover, the recent generation of plants lacking β-1,2-xylosyltransferase and α-1,3-fucosyltransferase activities (FX-KO), by CRISPR/Cas9 genome editing, enables production of proteins with "humanized" N-glycosylation. In this study, we investigated the impact of plant N-glycosylation on the immunogenic properties of a chimeric HBV S/L vaccine candidate produced in wild-type and FX-KO Nicotiana benthamiana. Prevention of β-1,2-xylose and α-1,3-fucose attachment to the HBV antigen significantly increased the immune response in mice, as compared with the wild-type plant-produced counterpart. Notably, the antibodies triggered by the FX-KO-made antigen neutralized more efficiently both wild-type HBV and a clinically relevant vaccine escape mutant. Our study validates in premiere the glyco-engineered Nicotiana benthamiana as a substantially improved host for plant production of glycoprotein vaccines.
MeSH term(s)	Humans ; Animals ; Mice ; Hepatitis B virus/genetics ; Glycosylation ; Nicotiana/genetics ; CRISPR-Cas Systems/genetics ; COVID-19/genetics ; SARS-CoV-2 ; Hepatitis B Vaccines/genetics ; Antibodies, Neutralizing ; Hepatitis B Surface Antigens/genetics
Chemical Substances	Hepatitis B Vaccines ; Antibodies, Neutralizing ; Hepatitis B Surface Antigens
Language	English
Publishing date	2023-03-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2136367-5
ISSN	1467-7652 ; 1467-7652
ISSN (online)	1467-7652
ISSN	1467-7652
DOI	10.1111/pbi.14028
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Article ; Online: Description of Culicoides truuskae sp. n. (Diptera

Karien Labuschagne / Rudolf Meiswinkel† / Danica Liebenberg / Carissa van Zyl / Antoinette van Schalkwyk / Clarke Scholtz

Onderstepoort Journal of Veterinary Research, Vol 90, Iss 1, Pp e1-e

Ceratopogonidae) from southern Africa

2023 Volume 14

Abstract	Culicoides truuskae Labuschagne and Meiswinkel sp. n. is described and illustrated in both sexes from material collected in South Africa and Namibia. It is restricted to the xeric western margin of the subcontinent, occurring in Fynbos, Nama-Karoo and Succulent Karoo ecoregions in South Africa and Desert and Savanna ecoregions in Namibia experiencing 600 mm of rainfall annually. Culicoides truuskae sp. n. is part of the Afrotropical ‘plain-wing’ Culicoides in which the wing lacks a distinguishing pattern of light and dark spots; the diagnostic dark smudge that traverses wing cell r3 may result in C. truuskae sp. n. being misidentified as the sympatric but phyletically unrelated Culicoides herero (Enderlein) – (of the Similis group, subgenus Oecacta Poey). Additionally, this study is the first description of the male of C. herero. C. truuskae sp. n. and Culicoides coarctatus Clastrier and Wirth share similar characters in the male genitalia, although the two species are separable on wing pattern and female flagellum sensilla coeloconica (SCo) distribution. The breeding habitat and adult female blood-feeding preferences of C. truuskae sp. n. are not known. A maximum likelihood phylogenetic tree, using mitochondrial cytochrome c oxidase I (COI) sequence data, is provided to further clarify the relationship between C. truuskae sp. n., C. coarctatus and C. herero. Extensive light trap data, collected over 30 years, are used to map the distribution ranges of C. truuskae sp. n., C. coarctatus and C. herero in Southern Africa. Contribution: The description of this new species and the description of the male of C. herero increases our understanding of the diversity and distribution of Culicoides species in southern Africa.
Keywords	culicoides ; taxonomy ; description new species ; dna barcoding ; map ; Veterinary medicine ; SF600-1100
Subject code	590
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	AOSIS
Document type	Article ; Online
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Article ; Online: N-glycoproteomics of brain synapses and synaptic vesicles

Mazdak M. Bradberry / Trenton M. Peters-Clarke / Evgenia Shishkova / Edwin R. Chapman / Joshua J. Coon

Cell Reports, Vol 42, Iss 4, Pp 112368- (2023)

2023

Abstract: ... for robust neurotransmission. Asparagine (N)-linked glycosylation is required for delivery of the major SV ... glycoproteins synaptophysin and SV2A to SVs. Despite this key role for N-glycosylation, the molecular ... compositions of SV N-glycans are largely unknown. In this study, we combined organelle isolation techniques and ...

Abstract	Summary: At mammalian neuronal synapses, synaptic vesicle (SV) glycoproteins are essential for robust neurotransmission. Asparagine (N)-linked glycosylation is required for delivery of the major SV glycoproteins synaptophysin and SV2A to SVs. Despite this key role for N-glycosylation, the molecular compositions of SV N-glycans are largely unknown. In this study, we combined organelle isolation techniques and high-resolution mass spectrometry to characterize N-glycosylation at synapses and SVs from mouse brain. Detecting over 2,500 unique glycopeptides, we found that SVs harbor a distinct population of oligomannose and highly fucosylated N-glycans. Using complementary fluorescence methods, we identify at least one highly fucosylated N-glycan enriched in SVs compared with synaptosomes. High fucosylation was characteristic of SV proteins, plasma membrane proteins, and cell adhesion molecules with key roles in synaptic function and development. Our results define the N-glycoproteome of a specialized neuronal organelle and inform timely questions in the glycobiology of synaptic pruning and neuroinflammation.
Keywords	CP: Neuroscience ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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