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  1. Article ; Online: Vaping-Dependent Pulmonary Inflammation Is Ca

    Shipman, Jeffrey G / Onyenwoke, Rob U / Sivaraman, Vijay

    International journal of molecular sciences

    2024  Volume 25, Issue 3

    Abstract: Here we use the SCIREQ InExpose system to simulate a biologically relevant vaping model in mice to investigate the role of calcium signaling in vape-dependent pulmonary disease as well as to investigate if there is a gender-based difference of disease. ... ...

    Abstract Here we use the SCIREQ InExpose system to simulate a biologically relevant vaping model in mice to investigate the role of calcium signaling in vape-dependent pulmonary disease as well as to investigate if there is a gender-based difference of disease. Male and female mice were vaped with JUUL Menthol (3% nicotine) using the SCIREQ InExpose system for 2 weeks. Additionally, 2-APB, a known calcium signaling inhibitor, was administered as a prophylactic for lung disease and damage caused by vaping. After 2 weeks, mice were exposed to lipopolysaccharide (LPS) to mimic a bacterial infection. Post-infection (24 h), mice were sacrificed, and bronchoalveolar lavage fluid (BALF) and lungs were taken. Vaping primed the lungs for worsened disease burden after microbial challenge (LPS) for both males and females, though females presented increased neutrophilia and inflammatory cytokines post-vape compared to males, which was assessed by flow cytometry, and cytokine and histopathological analysis. This increased inflammatory burden was controlled by calcium signaling inhibition, suggesting that calcium dysregulation may play a role in lung injury caused by vaping in a gender-dependent manner.
    MeSH term(s) Male ; Female ; Mice ; Animals ; Vaping/adverse effects ; Lipopolysaccharides/toxicity ; Pneumonia/etiology ; Pneumonia/pathology ; Lung/pathology ; Bronchoalveolar Lavage Fluid ; Lung Diseases ; Cytokines ; Inflammation
    Chemical Substances Lipopolysaccharides ; Cytokines
    Language English
    Publishing date 2024-02-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25031785
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Calcium Signaling Derangement and Disease Development and Progression.

    Sivaraman, Vijay / Onyenwoke, Rob U

    Biology

    2021  Volume 10, Issue 4

    Abstract: The importance of intracellular calcium ( ... ...

    Abstract The importance of intracellular calcium (Ca
    Language English
    Publishing date 2021-04-02
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology10040291
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: E-cigarette Flavors, Sensory Perception, and Evoked Responses.

    Alhadyan, Shatha K / Sivaraman, Vijay / Onyenwoke, Rob U

    Chemical research in toxicology

    2022  Volume 35, Issue 12, Page(s) 2194–2209

    Abstract: The chemosensory experiences evoked by flavors encompass a number of unique sensations that include olfactory stimuli (smell), gustatory stimuli (taste, i.e., salty, sweet, sour, bitter, and umami (also known as "savoriness")), and chemesthesis (touch). ... ...

    Abstract The chemosensory experiences evoked by flavors encompass a number of unique sensations that include olfactory stimuli (smell), gustatory stimuli (taste, i.e., salty, sweet, sour, bitter, and umami (also known as "savoriness")), and chemesthesis (touch). As such, the responses evoked by flavors are complex and, as briefly stated above, involve multiple perceptive mechanisms. The practice of adding flavorings to tobacco products dates back to the 17th century but is likely much older. More recently, the electronic cigarette or "e-cigarette" and its accompanying flavored e-liquids emerged on to the global market. These new products contain no combustible tobacco but often contain large concentrations (reported from 0 to more than 50 mg/mL) of nicotine as well as numerous flavorings and/or flavor chemicals. At present, there are more than 400 e-cigarette brands available along with potentially >15,000 different/unique flavored products. However, surprisingly little is known about the flavors/flavor chemicals added to these products, which can account for >1% by weight of some e-liquids, and their resultant chemosensory experiences, and the US FDA has done relatively little, until recently, to regulate these products. This article will discuss e-cigarette flavors and flavor chemicals, their elicited responses, and their sensory effects in some detail.
    MeSH term(s) Electronic Nicotine Delivery Systems ; Flavoring Agents ; Tobacco Products ; Nicotiana ; Sensation ; Perception ; Vaping
    Chemical Substances Flavoring Agents
    Language English
    Publishing date 2022-12-08
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 639353-6
    ISSN 1520-5010 ; 0893-228X
    ISSN (online) 1520-5010
    ISSN 0893-228X
    DOI 10.1021/acs.chemrestox.2c00268
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2320: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Calcium-Dependent Pulmonary Inflammation and Pharmacological Interventions and Mediators.

    Shipman, Jeffrey G / Onyenwoke, Rob U / Sivaraman, Vijay

    Biology

    2021  Volume 10, Issue 10

    Abstract: Pulmonary diseases present a significant burden worldwide and lead to severe morbidity and mortality. Lung inflammation caused by interactions with either viruses, bacteria or fungi is a prominent characteristic of many pulmonary diseases. Tobacco smoke ... ...

    Abstract Pulmonary diseases present a significant burden worldwide and lead to severe morbidity and mortality. Lung inflammation caused by interactions with either viruses, bacteria or fungi is a prominent characteristic of many pulmonary diseases. Tobacco smoke and E-cig use ("vaping") are considered major risk factors in the development of pulmonary disease as well as worsening disease prognosis. However, at present, relatively little is known about the mechanistic actions by which smoking and vaping may worsen the disease. One theory suggests that long-term vaping leads to Ca
    Language English
    Publishing date 2021-10-16
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology10101053
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Calcium Signaling Derangement and Disease Development and Progression

    Vijay Sivaraman / Rob U. Onyenwoke

    Biology, Vol 10, Iss 291, p

    2021  Volume 291

    Abstract: The importance of intracellular calcium (Ca 2+ ) in regulating integral biological functions such as cell division, cell motility, autophagy, apoptosis and gene transcription through its capacity as a ubiquitous second messenger is clear [.] ...

    Abstract The importance of intracellular calcium (Ca 2+ ) in regulating integral biological functions such as cell division, cell motility, autophagy, apoptosis and gene transcription through its capacity as a ubiquitous second messenger is clear [.]
    Keywords n/a ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Optimization and validation of a DYRK1A TR-FRET assay for high-throughput screening.

    Tarpley, Michael / Caligan, Thomas B / Onyenwoke, Rob U / Williams, Kevin P

    MethodsX

    2021  Volume 8, Page(s) 101383

    Abstract: Due to its role in brain development, the DYRK1A kinase (dual-specificity tyrosine phosphorylation-regulated kinase 1a) has been proposed as a drug target for Down syndrome, and diseases associated with neurodegeneration including Alzheimer's and ... ...

    Abstract Due to its role in brain development, the DYRK1A kinase (dual-specificity tyrosine phosphorylation-regulated kinase 1a) has been proposed as a drug target for Down syndrome, and diseases associated with neurodegeneration including Alzheimer's and Parkinson's. Other diseases in which DYRK1A is implicated include cancer and diabetes. Hence, there is need for potent and selective DYRK1A inhibitors. To screen large diversity compound libraries versus DYRK1A requires the development of a cost-effective high-throughput screen. In this study, we have taken a commercial time-resolved fluorescence energy transfer (TR-FRET)-based assay for DYRK1A and optimized for smaller volumes and homogenous format at room temperature. Tracer and enzyme concentrations were determined. DYRK1A-GST, anti-GST Ab and tracer were pre-combined and total assay volume reduced 2-fold. The assay was validated using whole plate minimum and maximum signal wells with a Z' of 0.7-0.8 determined. Overall, this method:•Results in an optimized low volume, homogenous and validated assay for DYRK1A.•Delivers a cost effective high-throughput assay format for DYRK1A inhibitor screening.
    Language English
    Publishing date 2021-05-13
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2830212-6
    ISSN 2215-0161
    ISSN 2215-0161
    DOI 10.1016/j.mex.2021.101383
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Calcium Signaling Derangement and Disease Development and Progression

    Sivaraman, Vijay / Onyenwoke, Rob U

    Biology. 2021 Apr. 02, v. 10, no. 4

    2021  

    Abstract: The importance of intracellular calcium (Ca²⁺) in regulating integral biological functions such as cell division, cell motility, autophagy, apoptosis and gene transcription through its capacity as a ubiquitous second messenger is clear [ ... ] ...

    Abstract The importance of intracellular calcium (Ca²⁺) in regulating integral biological functions such as cell division, cell motility, autophagy, apoptosis and gene transcription through its capacity as a ubiquitous second messenger is clear [...]
    Keywords apoptosis ; autophagy ; calcium ; cell division ; cell movement ; transcription (genetics)
    Language English
    Dates of publication 2021-0402
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology10040291
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Lysosomal Storage Diseases-Regulating Neurodegeneration.

    Onyenwoke, Rob U / Brenman, Jay E

    Journal of experimental neuroscience

    2016  Volume 9, Issue Suppl 2, Page(s) 81–91

    Abstract: Autophagy is a complex pathway regulated by numerous signaling events that recycles macromolecules and can be perturbed in lysosomal storage diseases (LSDs). The concept of LSDs, which are characterized by aberrant, excessive storage of cellular material ...

    Abstract Autophagy is a complex pathway regulated by numerous signaling events that recycles macromolecules and can be perturbed in lysosomal storage diseases (LSDs). The concept of LSDs, which are characterized by aberrant, excessive storage of cellular material in lysosomes, developed following the discovery of an enzyme deficiency as the cause of Pompe disease in 1963. Great strides have since been made in better understanding the biology of LSDs. Defective lysosomal storage typically occurs in many cell types, but the nervous system, including the central nervous system and peripheral nervous system, is particularly vulnerable to LSDs, being affected in two-thirds of LSDs. This review provides a summary of some of the better characterized LSDs and the pathways affected in these disorders.
    Language English
    Publishing date 2016-04-05
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2659991-0
    ISSN 1179-0695
    ISSN 1179-0695
    DOI 10.4137/JEN.S25475
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Data supporting a pilot high-throughput screen of a drug library for identification of DYRK1A inhibitors and high-content imaging analysis of identified harmine analogs.

    Tarpley, Michael / Oladapo, Helen / Caligan, Thomas B / Onyenwoke, Rob U / Williams, Kevin P

    Data in brief

    2021  Volume 37, Page(s) 107189

    Abstract: The data presented in this article support the accompanying research article "Identification of harmine and β-carboline analogs from a high-throughput screen of an approved drug collection; profiling as differential inhibitors of DYRK1A and monoamine ... ...

    Abstract The data presented in this article support the accompanying research article "Identification of harmine and β-carboline analogs from a high-throughput screen of an approved drug collection; profiling as differential inhibitors of DYRK1A and monoamine oxidase A and for in vitro and in vivo anti-cancer studies" [1]. As DYRK1A (dual-specificity tyrosine phosphorylation-regulated kinase 1a) plays a role in the pathophysiology of a number of diseases including diabetes, cancer and neurodegeneration [2], [3], [4], the identification of DYRK1A inhibitors is of significant interest. This data article details the hits identified from a DYRK1A high-throughput screen of a small molecule compound library containing over 95% approved drugs. Twenty-two compounds were identified with >50% inhibition, including harmine and four of its analogs. Subsequent profiling of these harmine analogs using glioma cancer cell lines and high-content image analysis identified those with effects on growth and cytotoxicity.
    Language English
    Publishing date 2021-05-30
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2786545-9
    ISSN 2352-3409 ; 2352-3409
    ISSN (online) 2352-3409
    ISSN 2352-3409
    DOI 10.1016/j.dib.2021.107189
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Vaping Exacerbates Coronavirus-Related Pulmonary Infection in a Murine Model.

    Sivaraman, Vijay / Parker, De'Jana / Zhang, Rui / Jones, Myles M / Onyenwoke, Rob U

    Frontiers in physiology

    2021  Volume 12, Page(s) 634839

    Abstract: Though the current preponderance of evidence indicates the toxicity associated with the smoking of tobacco products through conventional means, less is known about the role of "vaping" in respiratory disease. "Vaping" is described as the use of ... ...

    Abstract Though the current preponderance of evidence indicates the toxicity associated with the smoking of tobacco products through conventional means, less is known about the role of "vaping" in respiratory disease. "Vaping" is described as the use of electronic cigarettes (E-Cigarettes or E-Cigs), which has only more recently been available to the public (∼10 years) but has quickly emerged as a popular means of tobacco consumption worldwide. The World Health Organization (WHO) declared the SARS-CoV-2 outbreak as a global pandemic in March 2020. SARS-CoV-2 can easily be transmitted between people in close proximity through direct contact or respiratory droplets to develop coronavirus infectious disease 2019 (COVID-19). Symptoms of COVID-19 range from a mild flu-like illness with high fever to severe respiratory distress syndrome and death. The risk factors for increased disease severity remain unclear. Herein, we utilize a murine-tropic coronavirus (beta coronavirus) MHV-A59 along with a mouse model and measures of pathology (lung weight/dry ratios and histopathology) and inflammation (ELISAs and cytokine array panels) to examine whether vaping may exacerbate the pulmonary disease severity of coronavirus disease. While vaping alone did result in some noted pathology, mice exposed with intranasal vaped e-liquid suffered more severe mortality due to pulmonary inflammation than controls when exposed to coronavirus infection. Our data suggest a role for vaping in increased coronavirus pulmonary disease in a mouse model. Furthermore, our data indicate that disease exacerbation may involve calcium (Ca
    Language English
    Publishing date 2021-05-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2021.634839
    Database MEDical Literature Analysis and Retrieval System OnLINE

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