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  1. Article ; Online: Purification and biochemical characterization of novel α-amylase and cellulase from

    Rajesh, Rekha / Gummadi, Sathyanarayana N

    Preparative biochemistry & biotechnology

    2023  , Page(s) 1–13

    Abstract: ... ...

    Abstract Bacillus
    Language English
    Publishing date 2023-12-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1322522-4
    ISSN 1532-2297 ; 1082-6068
    ISSN (online) 1532-2297
    ISSN 1082-6068
    DOI 10.1080/10826068.2023.2288574
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    Zs.A 821: Show issues Location:
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  2. Article ; Online: Regulation and functions of membrane lipids: Insights from

    Koyiloth, Muhasin / Gummadi, Sathyanarayana N

    BBA advances

    2022  Volume 2, Page(s) 100043

    Abstract: ... ...

    Abstract The
    Language English
    Publishing date 2022-01-15
    Publishing country Netherlands
    Document type Journal Article ; Review
    ISSN 2667-1603
    ISSN (online) 2667-1603
    DOI 10.1016/j.bbadva.2022.100043
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  3. Article ; Online: Phospholipid scramblase 3: a latent mediator connecting mitochondria and heavy metal apoptosis.

    Palanirajan, Santosh Kumar / Gummadi, Sathyanarayana N

    Cell biochemistry and biophysics

    2023  Volume 81, Issue 3, Page(s) 443–458

    Abstract: Lead and mercury are the ubiquitous heavy metals triggering toxicity and initiating apoptosis in cells. Though the toxic effects of heavy metals on various organs are known, there is a paucity of information on the mechanisms that instigate the current ... ...

    Abstract Lead and mercury are the ubiquitous heavy metals triggering toxicity and initiating apoptosis in cells. Though the toxic effects of heavy metals on various organs are known, there is a paucity of information on the mechanisms that instigate the current study. A plausible role of phospholipid scramblase 3 (PLSCR3) in Pb
    MeSH term(s) Humans ; Phospholipid Transfer Proteins/metabolism ; Phospholipid Transfer Proteins/pharmacology ; HEK293 Cells ; Lead/metabolism ; Lead/pharmacology ; Mitochondria/metabolism ; Metals, Heavy/toxicity ; Metals, Heavy/metabolism ; Apoptosis ; Mercury/toxicity ; Mercury/metabolism ; Reactive Oxygen Species/metabolism
    Chemical Substances Phospholipid Transfer Proteins ; Lead (2P299V784P) ; Metals, Heavy ; Mercury (FXS1BY2PGL) ; Reactive Oxygen Species
    Language English
    Publishing date 2023-06-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1357904-6
    ISSN 1559-0283 ; 1085-9195
    ISSN (online) 1559-0283
    ISSN 1085-9195
    DOI 10.1007/s12013-023-01145-0
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    Zs.A 1498: Show issues Location:
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  4. Article ; Online: Advances in the applications of Bacteriophages and phage products against food-contaminating bacteria.

    E, Suja / Gummadi, Sathyanarayana N

    Critical reviews in microbiology

    2023  , Page(s) 1–26

    Abstract: Food-contaminating bacteria pose a threat to food safety and the economy by causing foodborne illnesses and spoilage. Bacteriophages, a group of viruses that infect only bacteria, have the potential to control bacteria throughout the "farm-to-fork ... ...

    Abstract Food-contaminating bacteria pose a threat to food safety and the economy by causing foodborne illnesses and spoilage. Bacteriophages, a group of viruses that infect only bacteria, have the potential to control bacteria throughout the "farm-to-fork continuum". Phage application offers several advantages, including targeted action against specific bacterial strains and minimal impact on the natural microflora of food. This review covers multiple aspects of bacteriophages applications in the food industry, including their use as biocontrol and biopreservation agents to fight over 20 different genera of food-contaminating bacteria, reduce cross-contamination and the risk of foodborne diseases, and also to prolong shelf life and preserve freshness. The review also highlights the benefits of using bacteriophages in bioprocesses to selectively inhibit undesirable bacteria, such as substrate competitors and toxin producers, which is particularly valuable in complex microbial bioprocesses where physical or chemical methods become inadequate. Furthermore, the review briefly discusses other uses of bacteriophages in the food industry, such as sanitizing food processing environments and detecting specific bacteria in food products. The review also explores strategies to enhance the effectiveness of phages, such as employing multi-phage cocktails, encapsulated phages, phage products, and synergistic hurdle approaches by combining them with antimicrobials.
    Language English
    Publishing date 2023-10-20
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1053620-6
    ISSN 1549-7828 ; 1040-841X
    ISSN (online) 1549-7828
    ISSN 1040-841X
    DOI 10.1080/1040841X.2023.2271098
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    Zs.A 1025: Show issues Location:
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  5. Article ; Online: Interaction of human phospholipid scramblase 1 with cholesterol via CRAC motif is essential for functional regulation and subcellular localization.

    Koyiloth, Muhasin / Gummadi, Sathyanarayana N

    International journal of biological macromolecules

    2022  Volume 209, Issue Pt A, Page(s) 850–857

    Abstract: Human phospholipid scramblase 1 (hPLSCR1) possesses a putative cholesterol binding CRAC (cholesterol interaction/recognition amino acid consensus) motif at the C-terminal. The CRAC motif of hPLSCR1 interacts with cholesterol with an energy of interaction ...

    Abstract Human phospholipid scramblase 1 (hPLSCR1) possesses a putative cholesterol binding CRAC (cholesterol interaction/recognition amino acid consensus) motif at the C-terminal. The CRAC motif of hPLSCR1 interacts with cholesterol with an energy of interaction -64.39 KJ mol
    MeSH term(s) Cell Membrane/metabolism ; Cholesterol/metabolism ; Humans ; Membrane Microdomains/metabolism ; Phospholipid Transfer Proteins/chemistry ; Phospholipid Transfer Proteins/genetics ; Phospholipid Transfer Proteins/metabolism
    Chemical Substances PLSCR1 protein, human ; Phospholipid Transfer Proteins ; Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2022-04-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2022.04.087
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    Zs.B 2374: Show issues Location:
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  6. Article ; Online: An overview of mammalian and microbial hormone-sensitive lipases (lipolytic family IV): biochemical properties and industrial applications.

    Nagaroor, Vijayalakshmi / Gummadi, Sathyanarayana N

    Biotechnology & genetic engineering reviews

    2022  , Page(s) 1–30

    Abstract: In mammals, hormone-sensitive lipase (EC 3.1.1.79) is an intracellular lipase that significantly regulates lipid metabolism. Mammalian HSL is more active towards diacylglycerol but lacks a lid covering the active site. Dyslipidemia, hepatic steatosis, ... ...

    Abstract In mammals, hormone-sensitive lipase (EC 3.1.1.79) is an intracellular lipase that significantly regulates lipid metabolism. Mammalian HSL is more active towards diacylglycerol but lacks a lid covering the active site. Dyslipidemia, hepatic steatosis, cancer, and cancer-associated cachexia are symptoms of HSL pathophysiology. Certain microbial proteins show a sequence homologous to the catalytic domain of mammalian HSL, hence called microbial HSL. They possess a funnel-shaped substrate-binding pocket and restricted length of acyl chain esters, thus known as esterases. These enzymes have broad substrate specificities and are capable of stereo, regio, and enantioselective, making them attractive biocatalysts in a wide range of industrial applications in the production of flavors, pharmaceuticals, biosensors, and fine chemicals. This review will provide insight into mammalian and microbial HSLs, their sources, structural features related to substrate specificity, thermal stability, and their applications.
    Language English
    Publishing date 2022-09-25
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 49457-4
    ISSN 2046-5556 ; 0264-8725
    ISSN (online) 2046-5556
    ISSN 0264-8725
    DOI 10.1080/02648725.2022.2127071
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  7. Article ; Online: Biophysical characterization of the DNA binding motif of human phospholipid scramblase 1.

    Rayala, Sarika / Sivagnanam, Ulaganathan / Gummadi, Sathyanarayana N

    European biophysics journal : EBJ

    2022  Volume 51, Issue 7-8, Page(s) 579–593

    Abstract: Human phospholipid scramblase 1 (hPLSCR1) is a 37 kDa multi-compartmental protein, which was initially identified as a ... ...

    Abstract Human phospholipid scramblase 1 (hPLSCR1) is a 37 kDa multi-compartmental protein, which was initially identified as a Ca
    MeSH term(s) Humans ; Phospholipid Transfer Proteins/genetics ; Phospholipid Transfer Proteins/metabolism ; Phospholipids/metabolism ; Cell Membrane/metabolism ; Protein Domains ; Oligonucleotides
    Chemical Substances Phospholipid Transfer Proteins ; Phospholipids ; Oligonucleotides
    Language English
    Publishing date 2022-10-19
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 283671-3
    ISSN 1432-1017 ; 0175-7571
    ISSN (online) 1432-1017
    ISSN 0175-7571
    DOI 10.1007/s00249-022-01621-0
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    Zs.A 1121: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Article ; Online: α-Amylase and cellulase production by novel halotolerant Bacillus sp.PM06 isolated from sugarcane pressmud.

    Rajesh, Rekha / Gummadi, Sathyanarayana N

    Biotechnology and applied biochemistry

    2021  Volume 69, Issue 1, Page(s) 149–159

    Abstract: A novel Bacillus sp.PM06 isolated from sugarcane waste pressmud was tested for extracellular α-amylase and cellulase enzyme production. The effect of different substrates, nitrogen sources, pH, and temperature on growth and extracellular enzyme ... ...

    Abstract A novel Bacillus sp.PM06 isolated from sugarcane waste pressmud was tested for extracellular α-amylase and cellulase enzyme production. The effect of different substrates, nitrogen sources, pH, and temperature on growth and extracellular enzyme production was examined. Bacillus sp.PM06 was able to grow with starch and carboxymethyl cellulose (CMC) as a sole source of carbon and ammonium chloride was found to be the best nitrogen source. Maximum enzyme production was obtained at 48 H for both α-amylase and cellulase. The optimal condition for measuring enzyme activity was found to be pH 5.5 at 50 °C for α-amylase and pH 6.4 at 60 °C for cellulase respectively. It was found that Bacillus sp.PM06 exhibited halotolerance up to 2 M Sodium chloride (NaCl) and Potassium chloride (KCl). The isolate could produce α-amylase in the presence of 2 M NaCl and 1 M KCl. However, the strain produced cellulase even in the presence of 2 M NaCl and KCl. Concomitant production of both enzymes was observed when the medium was supplemented with starch and CMC. A maximum of 31 ± 1.15 U/mL of amylase and 15 ± 1.5 U/mL of cellulase was produced in 48 H. The enzyme was partially purified by Ammonium sulphate (NH
    MeSH term(s) Bacillus ; Cellulase ; Hydrogen-Ion Concentration ; Saccharum ; Temperature ; alpha-Amylases
    Chemical Substances alpha-Amylases (EC 3.2.1.1) ; Cellulase (EC 3.2.1.4)
    Language English
    Publishing date 2021-01-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 883433-7
    ISSN 1470-8744 ; 0885-4513
    ISSN (online) 1470-8744
    ISSN 0885-4513
    DOI 10.1002/bab.2091
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    Zs.A 1865: Show issues Location:
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  9. Article ; Online: Cholesterol interaction attenuates scramblase activity of SCRM-1 in the artificial membrane.

    Koyiloth, Muhasin / Gummadi, Sathyanarayana N

    Biochimica et biophysica acta. Biomembranes

    2021  Volume 1863, Issue 9, Page(s) 183548

    Abstract: Phospholipid (PL) scramblases are single-pass transmembrane protein mediating bidirectional PL translocation. Previously in silico analysis of human PL scramblases, predicted the presence of an uncharacterized cholesterol-binding domain spanning partly ... ...

    Abstract Phospholipid (PL) scramblases are single-pass transmembrane protein mediating bidirectional PL translocation. Previously in silico analysis of human PL scramblases, predicted the presence of an uncharacterized cholesterol-binding domain spanning partly in the transmembrane helix as well as in the adjacent extracellular coil. This domain was found to be universally conserved in diverse organisms like Caenorhabditis elegans. In this study, we investigated the saturable cholesterol-binding domain of SCRM-1 using fluorescence sterol binding assay, Stern-Volmer quenching, Förster resonance energy transfer, and CD spectroscopy. We observed high-affinity interaction between cholesterol and SCRM-1. Our results support a previous report, which showed that the cholesterol ordering effect reduced the scramblase activity of hPLSCR1. Considering the presence of a high-affinity binding sequence, we propose that the reduction in activity could partly be due to the cholesterol binding. To validate this, we generated a C-terminal helix (CTH) deletion construct (∆CTH SCRM-1) and a point mutation in the putative cholesterol-binding domain I273D SCRM-1. Deletion construct greatly reduced cholesterol affinity along with loss of scramblase activity. In contrast to this, I273D SCRM-1 retained scrambling activity in proteoliposomes containing ~30 mol% cholesterol but lost sterol binding ability. These results suggest that C-terminal helix is crucial for membrane insertion and in the lipid bilayer the scrambling activity of SCRM-1 is modulated through its interaction with cholesterol.
    MeSH term(s) Caenorhabditis elegans Proteins/chemistry ; Caenorhabditis elegans Proteins/metabolism ; Cholesterol/chemistry ; Cholesterol/metabolism ; Humans ; Membranes, Artificial ; Phospholipid Transfer Proteins/chemistry ; Phospholipid Transfer Proteins/metabolism
    Chemical Substances Caenorhabditis elegans Proteins ; Membranes, Artificial ; Phospholipid Transfer Proteins ; SCRM-1 protein, C elegans ; Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2021-01-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbamem.2020.183548
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    Uc I Zs.247: Show issues Location:
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  10. Article: Regulation and functions of membrane lipids: Insights from Caenorhabditis elegans

    Koyiloth, Muhasin / Gummadi, Sathyanarayana N.

    BBA advances. 2022, v. 2

    2022  

    Abstract: The Caenorhabditis elegans plasma membrane is composed of glycerophospholipids and sphingolipids with a small cholesterol. The C. elegans obtain the majority of the membrane lipids by modifying fatty acids present in the bacterial diet. The metabolic ... ...

    Abstract The Caenorhabditis elegans plasma membrane is composed of glycerophospholipids and sphingolipids with a small cholesterol. The C. elegans obtain the majority of the membrane lipids by modifying fatty acids present in the bacterial diet. The metabolic pathways of membrane lipid biosynthesis are well conserved across the animal kingdom. In C. elegans CDP-DAG and Kennedy pathway produce glycerophospholipids. Meanwhile, the sphingolipids are synthesized through a different pathway. They have evolved remarkably diverse mechanisms to maintain membrane lipid homeostasis. For instance, the lipid bilayer stress operates to accomplish homeostasis during any perturbance in the lipid composition. Meanwhile, the PAQR-2/IGLR-2 complex works with FLD-1 to balance unsaturated to saturated fatty acids to maintain membrane fluidity. The loss of membrane lipid homeostasis is observed in many human genetic and metabolic disorders. Since C. elegans conserved such genes and pathways, it can be used as a model organism.
    Keywords Caenorhabditis elegans ; biosynthesis ; cholesterol ; diet ; glycerophospholipids ; homeostasis ; humans ; lipid bilayers ; lipid composition ; membrane fluidity ; plasma membrane ; sphingolipids
    Language English
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 2667-1603
    DOI 10.1016/j.bbadva.2022.100043
    Database NAL-Catalogue (AGRICOLA)

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