LIVIVO - Search results -

Search results

Result 1 - 10 of total 1303

Search options

Article: Development of microsatellite markers for genes related to defense responses in Musa acuminata

Miller, R. N. G / F. L. Emediato / O. Jr. Silva / R. Togawa

Acta horticulturae. 2016 Mar. 22, , no. 1114

2016

Abstract: Banana (Musa spp.) is one of the world's most important monocotyledonous crops. As many of today's commercial cultivars are sterile triploids or diploids, with fruit development via parthenocarpy, restricted genetic variation has resulted in a crop ... ...

Abstract	Banana (Musa spp.) is one of the world's most important monocotyledonous crops. As many of today's commercial cultivars are sterile triploids or diploids, with fruit development via parthenocarpy, restricted genetic variation has resulted in a crop lacking disease resistance. Considering the importance of molecular tools to accelerate development of disease resistant genotypes, the objective of this study was to develop microsatellite markers in Musa acuminata for unigenes related to defense and response to biotic stress. Microsatellites were mined in sequence data using the programs WebSat and SSRIT, with flanking primers designed using Primer3. Analyzed data containing genes potentially involved in defense or stress responses comprised unigene sequences for M. acuminata genotypes 'Calcutta 4' and Cavendish cultivar 'Grande Naine' (AAA genome, Cavendish subgroup), derived from 454 transcriptome pyrosequencing, NBS-LRR family resistance gene analog (RGA)-containing DNA BAC clone sequences from M. acuminata 'Calcutta 4', and NB-ARC conserved domain sequences in the M. acuminata DH Pahang whole genome sequence. A total of 156 SSR loci were validated for polymorphism by PCR in 20 M. acuminata genotypes organized into DNA bulks contrasting in resistance to black leaf streak and Sigatoka leaf spot. Primer annealing temperatures and MgCl2 concentrations were optimized and polymorphism examined on 4% polyacrylamide gels with silver nitrate staining. Currently, approximately 16% of loci display polymorphism. These loci have been tested against individualized bulks for identification alleles for each genotype. The microsatellites identified, associated with genes potentially involved in defense or stress responses, are potentially useful genetic markers for genotyping and genetic map construction for Musa segregating populations.
Keywords	alleles ; bacterial artificial chromosomes ; bananas ; biotic stress ; crops ; cultivars ; diploidy ; disease resistance ; DNA ; gels ; genetic markers ; genetic resistance ; genetic variation ; genotype ; genotyping ; leaf spot ; leaves ; loci ; magnesium chloride ; microsatellite repeats ; Musa acuminata ; nucleic acid annealing ; parthenocarpy ; polyacrylamide ; polymerase chain reaction ; sequence analysis ; silver staining ; stress response ; temperature ; transcriptome ; triploidy ; unigenes
Language	English
Dates of publication	2016-0322
Size	p. 91-94.
Publishing place	International Society for Horticultural Science.
Document type	Article
Note	Paper presented at the XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and landscapes (IHC2014): IX International Symposium on Banana: ISHS-ProMusa Symposium on Unravelling the Banana's Genomic Potential held August 17-22, 2014, Brisbane, Australia
ISSN	0567-7572
DOI	10.17660/ActaHortic.2016.1114.12
Database	NAL-Catalogue (AGRICOLA)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Gene expression and proteome analysis during the Musa acuminata-Mycosphaerella musicola interaction

Miller, R. N. G / F. L. Emediato / M. A. N. Passos / N. G. Oliveira-Junior / O. Jr. Silva / O. L. Franco / V. O. Cruz

Acta horticulturae. 2016 Mar. 22, , no. 1114

2016

Abstract: Sigatoka leaf spot disease in banana (Musa), caused by the fungal pathogen Mycosphaerella musicola, can cause significant reduction in functional leaf area and premature fruit ripening. In order to develop a functional genomics resource for this crop ... ...

Abstract	Sigatoka leaf spot disease in banana (Musa), caused by the fungal pathogen Mycosphaerella musicola, can cause significant reduction in functional leaf area and premature fruit ripening. In order to develop a functional genomics resource for this crop which offers insights into molecular mechanisms involved in host responses to this biotic stress, an analysis of differential gene expression was conducted in 'Calcutta 4' (AA genome, Musa acuminata ssp. burmannicoides) and 'Grande Naine' (AAA genome, Cavendish subgroup), contrasting in resistance to the fungal pathogen. Total cDNA was prepared from whole plant leaf materials, both uninfected and artificially challenged with pathogen conidiospores. From a subset of defense-related genes identified in silico based upon available 454 GS-FLX Titanium-derived sequence transcripts, gene expression was further examined by RT-qPCR. Data were analyzed using programs Rest and Miner to calculate primer efficiency and differential gene expression. Transcripts displaying a significant change in expression between infected and non-infected treatments included, amongst others, chitinases, thaumatin, glutathione transferases, a putative avrRpt2-induced AIG2 protein, a Germin/oxalate oxidase, a regulator of pathogen resistance responses of RPS2 and RPM1 genes, and an IAA-amino acid hydrolase. Simultaneously, the proteomic profile for this pathosystem was conducted from the inoculated and non-inoculated leaf materials. Isoelectric focusing of proteins, followed by second-dimension separation using 12% SDS polyacrylamide gels, enabled differential protein spots to be identified for characterization by MALDI-TOF-TOF. Analysis of gene and protein expression in this pathosystem is important for furthering our understanding of defense mechanisms in Musa and development of disease control strategies.
Keywords	bananas ; biotic stress ; chitinase ; complementary DNA ; defense mechanisms ; fungi ; gene expression ; gene expression regulation ; genes ; genomics ; glutathione ; isoelectric focusing ; leaf area ; leaf spot ; leaves ; Musa acuminata subsp. burmannica ; Mycosphaerella musicola ; oxalate oxidase ; pathogens ; protein synthesis ; proteome ; proteomics ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; ripening ; transferases
Language	English
Dates of publication	2016-0322
Size	p. 149-152.
Publishing place	International Society for Horticultural Science.
Document type	Article
Note	Paper presented at the XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and landscapes (IHC2014): IX International Symposium on Banana: ISHS-ProMusa Symposium on Unravelling the Banana's Genomic Potential held August 17-22, 2014, Brisbane, Australia
ISSN	0567-7572
DOI	10.17660/ActaHortic.2016.1114.21
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Network science

Fredric M. Windsor / Dolors Armenteras / Ana Paula A. Assis / Julia Astegiano / Pamela C. Santana / Luciano Cagnolo / Luísa G. Carvalheiro / Clive Emary / Hugo Fort / Xavier I. Gonzalez / James J.N. Kitson / Ana C.F. Lacerda / Marcelo Lois / Viviana Márquez-Velásquez / Kirsten E. Miller / Marcos Monasterolo / Marina Omacini / Kate P. Maia / Tania Paula Palacios /

Michael J.O. Pocock / Santiago L. Poggio / Isabela G. Varassin / Diego P. Vázquez / Julia Tavella / Débora C. Rother / Mariano Devoto / Paulo R. Guimarães, Jr. / Darren M. Evans

Perspectives in Ecology and Conservation, Vol 20, Iss 2, Pp 79-

Applications for sustainable agroecosystems and food security

2022 Volume 90

Abstract: The global challenge of feeding two billion more people by 2050, using more sustainable agricultural practices whilst dealing with uncertainties associated with environmental change, requires a transformation of food systems. We present a new perspective ...

Abstract	The global challenge of feeding two billion more people by 2050, using more sustainable agricultural practices whilst dealing with uncertainties associated with environmental change, requires a transformation of food systems. We present a new perspective for how advances in network science can provide novel ways to better understand, harness, and restore multiple ecological processes in agricultural environments. We describe: (i) a network-focused framework for managing agro-ecosystems that accounts for the multiple interactions between biodiversity and associated ecosystem services; (ii) guidance for incorporating socio-economic factors into ecological networks; and (iii) the potential to upscale network methods to inform efforts to build resilience, including global food-supply chains. In doing so we aim to facilitate the application of network science as a systems-based way to tackle the challenges of securing an equitable distribution of food.
Keywords	Agriculture ; Biodiversity ; Ecological networks ; Social networks ; Crops ; Food production ; Ecology ; QH540-549.5 ; General. Including nature conservation ; geographical distribution ; QH1-199.5
Subject code	333
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Mobile Lung Screening: Should We All Get on the Bus?

Headrick, James R / Morin, Olivia / Miller, Ashley D / Hill, Lauren / Smith, Jeremiah

The Annals of thoracic surgery

2020 Volume 110, Issue 4, Page(s) 1147–1152

Abstract: Background: Despite favorable recommendations, national lung screening adoption remains low (2% to 3%). Patients living in rural areas have additional challenges, including access to lung screening programs. We initiated a mobile lung screening program ... ...

Abstract	Background: Despite favorable recommendations, national lung screening adoption remains low (2% to 3%). Patients living in rural areas have additional challenges, including access to lung screening programs. We initiated a mobile lung screening program to serve the rural patients at risk. This is what we learned from this 12-month feasibility project. Methods: Utilizing a multidisciplinary approach, we began an 8-month design and build schedule. This was the first build of this type. The operational team included a radiology technician, nurse practitioner, driver with a commercial driver's license, and program developer. Specialized software was used for data mining. Downstream revenue projections were based on previously published Medicare claims data. Generally accepted accounting principles were used. Results: The prototype bus was delivered January 2018. During the 12-month feasibility period, we performed 548 low-dose lung screenings at 104 sites. Mean patient age was 62 years, mean pack-years of smoking was 41; 258 (47%) were male. Five lung cancers were found in addition to a type B thymoma. Financially, we exceeded the break-even analysis by 28%. The 5-year pro forma using 1 year of actual data and 4 additional years of projected data demonstrated a net present value of 1 million, internal rate of return of 34.6%, and profitability index of 2.2-all highly dependent on downstream revenue. Conclusions: Although challenges exist, a commercially viable bus and a financially sound mobile program can be developed. However, without a centralized approach for incidental findings, the downstream revenue may be at risk as well as the financial viability of the project.
MeSH term(s)	Aged ; Aged, 80 and over ; Cost-Benefit Analysis ; Early Detection of Cancer/economics ; Female ; Humans ; Lung Neoplasms/diagnosis ; Lung Neoplasms/economics ; Male ; Mass Screening/economics ; Mass Screening/methods ; Medicare/economics ; Middle Aged ; Mobile Health Units/economics ; Tomography, X-Ray Computed/methods ; United States
Language	English
Publishing date	2020-07-14
Publishing country	Netherlands
Document type	Journal Article ; Multicenter Study
ZDB-ID	211007-6
ISSN	1552-6259 ; 0003-4975
ISSN (online)	1552-6259
ISSN	0003-4975
DOI	10.1016/j.athoracsur.2020.03.093
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 252: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Clinical response to dabrafenib plus trametinib in a pediatric ganglioglioma with

Miller, Katherine E / Schieffer, Kathleen M / Grischow, Olivia / Rodriguez, Diana P / Cottrell, Catherine E / Leonard, Jeffrey R / Finlay, Jonathan L / Mardis, Elaine R

Cold Spring Harbor molecular case studies

2021 Volume 7, Issue 2

Abstract: In this follow-up report, we present updated information regarding a previously reported pediatric patient with a World Health Organization grade I ganglioglioma harboring ... ...

Abstract	In this follow-up report, we present updated information regarding a previously reported pediatric patient with a World Health Organization grade I ganglioglioma harboring a
MeSH term(s)	Adolescent ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Brain Neoplasms/diagnostic imaging ; Brain Neoplasms/drug therapy ; Brain Neoplasms/genetics ; Brain Neoplasms/pathology ; Cohort Studies ; Female ; Ganglioglioma/diagnostic imaging ; Ganglioglioma/drug therapy ; Ganglioglioma/genetics ; Ganglioglioma/pathology ; Humans ; Imidazoles/therapeutic use ; Mutation ; Oximes/therapeutic use ; Protein Kinase Inhibitors/therapeutic use ; Proto-Oncogene Proteins B-raf/genetics ; Pyridones/therapeutic use ; Pyrimidinones/therapeutic use ; Treatment Outcome
Chemical Substances	Imidazoles ; Oximes ; Protein Kinase Inhibitors ; Pyridones ; Pyrimidinones ; trametinib (33E86K87QN) ; BRAF protein, human (EC 2.7.11.1) ; Proto-Oncogene Proteins B-raf (EC 2.7.11.1) ; dabrafenib (QGP4HA4G1B)
Language	English
Publishing date	2021-04-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2835759-0
ISSN	2373-2873 ; 2373-2873
ISSN (online)	2373-2873
ISSN	2373-2873
DOI	10.1101/mcs.a006023
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Behavioral and Attitudinal Correlates of Trusted Sources of COVID-19 Vaccine Information in the US.

Latkin, Carl A / Dayton, Lauren / Miller, Jacob R / Yi, Grace / Jaleel, Afareen / Nwosu, Chikaodinaka C / Yang, Cui / Falade-Nwulia, Oluwaseun

Behavioral sciences (Basel, Switzerland)

2021 Volume 11, Issue 4

Abstract: There is a critical need for the public to have trusted sources of vaccine information. A longitudinal online study assessed trust in COVID-19 vaccine information from 10 sources. A factor analysis for data reduction revealed two factors. The first ... ...

Abstract	There is a critical need for the public to have trusted sources of vaccine information. A longitudinal online study assessed trust in COVID-19 vaccine information from 10 sources. A factor analysis for data reduction revealed two factors. The first factor contained politically conservative sources (PCS) of information. The second factor included eight news sources representing mainstream sources (MS). Multivariable logistic regression models were used. Trust in Dr. Fauci was also examined. High trust in MS was associated with intention to encourage family members to get COVID-19 vaccines, altruistic beliefs that more vulnerable people should have vaccine priority, and belief that racial minorities with higher rates of COVID-19 deaths should have priority. High trust in PCS was associated with intention to discourage friends from getting vaccinated. Higher trust in PCS was also associated with participants more likely to disagree that minorities with higher rates of COVID-19 deaths should have priority for a vaccine. High trust in Dr. Fauci as a source of COVID-19 vaccine information was associated with factors similar to high trust in MS. Fair, equitable, and transparent access and distribution are essential to ensure trust in public health systems' abilities to serve the population.
Language	English
Publishing date	2021-04-20
Publishing country	Switzerland
Document type	Journal Article
ISSN	2076-328X
ISSN	2076-328X
DOI	10.3390/bs11040056
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Key questions for modelling COVID-19 exit strategies

Thompson RN / Hollingsworth TD / Isham V / Arribas-Bel D / Ashby B / Britton T / Challenor P / Chappell LHK / Clapham H / Cunniffe NJ / Dawid AP / Donnelly CA / Eggo RM / Funk S / Gilbert N / Glendinning P / Gog JR / Hart WS / Heesterbeek H /

House T / Keeling M / Kiss IZ / Kretzschmar ME / Lloyd AL / McBryde ES / McCaw JM / McKinley TJ / Miller JC / Morris M / O'Neill PD / Parag KV / Pearson CAB / Pellis L / Pulliam JRC / Ross JV / Tomba GS / Silverman BW / Struchiner CJ / Tildesley MJ / Trapman P / Webb CR / Mollison D / Restif O

2020

Keywords	covid19
Publishing country	uk
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Accelerating Environmental Research to Achieve Sustainable Development Goals.

Mihelcic, James R / Barra, Ricardo O / Brooks, Bryan W / Diamond, Miriam L / Eckelman, Matthew J / MacDonald Gibson, Jacqueline / Guidotti, Sunny / Ikeda-Araki, Atsuko / Kumar, Manish / Maiga, Ynoussa / McConville, Jennifer / Miller, Shelly L / Pizarro, Valeria / Rosario-Ortiz, Fernando / Wang, Shuxiao / Zimmerman, Julie B

Environmental science & technology

2023 Volume 57, Issue 45, Page(s) 17167–17168

MeSH term(s)	Sustainable Development ; Conservation of Natural Resources ; Goals
Language	English
Publishing date	2023-11-02
Publishing country	United States
Document type	Editorial
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.3c08894
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Environmental Research Addressing Sustainable Development Goals.

Mihelcic, James R / Barra, Ricardo O / Brooks, Bryan W / Diamond, Miriam L / Eckelman, Matthew J / Gibson, Jacqueline MacDonald / Guidotti, Sunny / Ikeda-Araki, Atsuko / Kumar, Manish / Maiga, Ynoussa / McConville, Jennifer / Miller, Shelly L / Pizarro, Valeria / Rosario-Ortiz, Fernando / Wang, Shuxiao / Zimmerman, Julie B

Environmental science & technology

2023 Volume 57, Issue 9, Page(s) 3457–3460

MeSH term(s)	Sustainable Development ; Conservation of Natural Resources ; Goals
Language	English
Publishing date	2023-02-22
Publishing country	United States
Document type	Editorial
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.3c01070
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Gut epithelial Interleukin-17 receptor A signaling can modulate distant tumors growth through microbial regulation.

Chandra, Vidhi / Li, Le / Le Roux, Olivereen / Zhang, Yu / Howell, Rian M / Rupani, Dhwani N / Baydogan, Seyda / Miller, Haiyan D / Riquelme, Erick / Petrosino, Joseph / Kim, Michael P / Bhat, Krishna P L / White, James R / Kolls, Jay K / Pylayeva-Gupta, Yuliya / McAllister, Florencia

Cancer cell

2023 Volume 42, Issue 1, Page(s) 85–100.e6

Abstract: Microbes influence cancer initiation, progression and therapy responsiveness. IL-17 signaling contributes to gut barrier immunity by regulating microbes but also drives tumor growth. A knowledge gap remains regarding the influence of enteric IL-17-IL- ... ...

Abstract	Microbes influence cancer initiation, progression and therapy responsiveness. IL-17 signaling contributes to gut barrier immunity by regulating microbes but also drives tumor growth. A knowledge gap remains regarding the influence of enteric IL-17-IL-17RA signaling and their microbial regulation on the behavior of distant tumors. We demonstrate that gut dysbiosis induced by systemic or gut epithelial deletion of IL-17RA induces growth of pancreatic and brain tumors due to excessive development of Th17, primary source of IL-17 in human and mouse pancreatic ductal adenocarcinoma, as well as B cells that circulate to distant tumors. Microbial dependent IL-17 signaling increases DUOX2 signaling in tumor cells. Inefficacy of pharmacological inhibition of IL-17RA is overcome with targeted microbial ablation that blocks the compensatory loop. These findings demonstrate the complexities of IL-17-IL-17RA signaling in different compartments and the relevance for accounting for its homeostatic host defense function during cancer therapy.
MeSH term(s)	Mice ; Animals ; Humans ; Interleukin-17 ; Receptors, Interleukin-17/genetics ; Mice, Knockout ; Signal Transduction ; Pancreatic Neoplasms/pathology
Chemical Substances	Interleukin-17 ; Receptors, Interleukin-17
Language	English
Publishing date	2023-12-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2078448-X
ISSN	1878-3686 ; 1535-6108
ISSN (online)	1878-3686
ISSN	1535-6108
DOI	10.1016/j.ccell.2023.12.006
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5650: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 104: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito