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  1. Article ; Online: Therapeutic effect and mechanism of Yougui Wan in rats with intervertebral disk degeneration.

    Ma, She / Liu, Kan / Yang, Jing-Yan / Huang, Ren-Jun / Yu, Dong

    Journal of orthopaedic surgery and research

    2024  Volume 19, Issue 1, Page(s) 89

    Abstract: Objective: To explore the potential mechanism of Yougui Wan on deformed lumbar intervertebral disk ... administered Yougui Wan by gavage for 2 consecutive weeks. Serum interleukin-6 (IL-10 ... increased, and the protein expression levels of Notch1 were decreased.: Conclusion: Yougui Wan ...

    Abstract Objective: To explore the potential mechanism of Yougui Wan on deformed lumbar intervertebral disk structure in rats.
    Methods: Thirty male Sprague-Dawley rats were randomly divided into 3 groups, with 10 rats in each group. The animals in the blank control group were healthy rats without specific treatment, and those in the model group and traditional Chinese medicine (TCM) group were used to establish the intervertebral disk degeneration (IDD) model by puncturing the annulus. Four weeks after modeling, rats in the TCM group were administered Yougui Wan by gavage for 2 consecutive weeks. Serum interleukin-6 (IL-10), macrophage migration inhibitory factor (MIF) and tumor necrosis factor alpha (TNF-α) levels were measured by ELISA, and the protein expression levels of collagen II and Notch1 in intervertebral disk tissues were examined by Western blotting. Apoptosis was detected by the TUNEL method.
    Results: Compared with those in the blank group, IL-10, MIF and TNF-α levels in the model group and TCM group were increased (P < 0.05), the protein expression levels of collagen II were decreased, and the protein expression levels of Notch1 were increased. Compared with those in the model group, the levels of IL-10 in the TCM group were increased (P < 0.05), the levels of MIF and TNF-α were decreased (P < 0.05), the protein expression levels of collagen II were increased, and the protein expression levels of Notch1 were decreased.
    Conclusion: Yougui Wan can inhibit the inflammatory response in IDD rats, reduce the degradation of extracellular matrix, reduce apoptosis in nucleus pulposus cells, and alleviate intervertebral disk degeneration. The mechanism may be related to the regulation of the Notch signaling pathway.
    MeSH term(s) Male ; Rats ; Animals ; Intervertebral Disc Degeneration/drug therapy ; Interleukin-10 ; Rats, Sprague-Dawley ; Tumor Necrosis Factor-alpha ; Collagen ; Drugs, Chinese Herbal
    Chemical Substances yougui ; Interleukin-10 (130068-27-8) ; Tumor Necrosis Factor-alpha ; Collagen (9007-34-5) ; Drugs, Chinese Herbal
    Language English
    Publishing date 2024-01-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 2252548-8
    ISSN 1749-799X ; 1749-799X
    ISSN (online) 1749-799X
    ISSN 1749-799X
    DOI 10.1186/s13018-024-04554-w
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  2. Article ; Online: Jiao-tai-wan and its effective component-berberine improve diabetes and depressive disorder through the cAMP/PKA/CREB signaling pathway.

    Tang, Yueheng / Gao, Yang / Nie, Kexin / Wang, Hongzhan / Chen, Shen / Su, Hao / Huang, Wenya / Dong, Hui

    Journal of ethnopharmacology

    2024  Volume 324, Page(s) 117829

    Abstract: Ethnopharmacological relevance: Jiao-tai-wan (JTW), a classic herbal formula ...

    Abstract Ethnopharmacological relevance: Jiao-tai-wan (JTW), a classic herbal formula of traditional Chinese medicine recorded in Han Shi Yi Tong, has been used to alleviate sleep disorders since ancient times. In modern pharmacological research, JTW has been adopted for treating diabetes mellitus and even exerts antidepressant effects. However, the potential mechanisms deserve further elucidation.
    Aim of the study: The prevalence of diabetes mellitus combined with depressive disorder (DD) is continuing to increase, yet it is currently under-recognized and its treatment remains inadequate. The present study aims to explore the underlying therapeutics and mechanisms of JTW on DD.
    Materials and methods: Chronic restraint stress was used on db/db mice to construct a mouse model of DD. The therapeutic effects of JTW were assessed by glucolipid metabolic indexes, behavioral tests, and depression-related neurotransmitter levels. The inflammatory status and cell apoptosis of different mice were investigated and the changes in the cAMP/PKA/CREB pathway were detected. Combining the results of fingerprinting with molecular docking, the active components of JTW were screened. A cellular model was constructed by intervention of glucose combined with corticosterone (CORT). The levels of apoptosis and depression-related neurotransmitters in HT-22 cells were examined, and the changes in the cAMP/PKA/CREB pathway were tested. Finally, the activator and inhibitor of the PKA protein were used for reverse validation experiments.
    Results: JTW could improve the impaired glucose tolerance, lipid metabolism disorders, and depression-like symptoms in DD mice. Meanwhile, JTW could alleviate the inflammatory status, suppress the microglia activation, and improve hippocampal neuron apoptosis in DD mice. The dual effects of JTW might be associated with the activation of the cAMP/PKA/CREB pathway. Berberine (Ber) was identified for the in vitro experiment, it could reverse the apoptosis of HT-22 cells and up-regulate the depression-related neurotransmitter levels, and the effects of Ber were related to the activation of the cAMP/PKA/CREB pathway as well.
    Conclusion: JTW could exert both hypoglycemic and antidepressant effects through activating the cAMP/PKA/CREB signaling pathway, its active component, Ber, could improve the damage to HT-22 cells induced by glucose combined with CORT via the activation of the cAMP/PKA/CREB pathway. Ber may be one of the effective components of the dual effects of JTW.
    MeSH term(s) Mice ; Animals ; Berberine/pharmacology ; Berberine/therapeutic use ; Molecular Docking Simulation ; Signal Transduction ; Diabetes Mellitus/drug therapy ; Antidepressive Agents/pharmacology ; Antidepressive Agents/therapeutic use ; Glucose/metabolism ; Depressive Disorder/drug therapy ; Neurotransmitter Agents ; Drugs, Chinese Herbal
    Chemical Substances jiao tai wan ; Berberine (0I8Y3P32UF) ; Antidepressive Agents ; Glucose (IY9XDZ35W2) ; Neurotransmitter Agents ; Drugs, Chinese Herbal
    Language English
    Publishing date 2024-02-01
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2024.117829
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  3. Article ; Online: The active components and potential mechanisms of Wuji Wan in the treatment of ethanol-induced gastric ulcer: An integrated metabolomics, network pharmacology and experimental validation.

    Wu, Tiantai / Zhang, Huan / Jin, Yang / Zhang, Ming / Zhao, Qing / Li, Herong / Wang, Shouli / Lu, Yuan / Chen, Shuaishuai / Du, Huakang / Liu, Ting / Guo, Weiyu / Liu, Wen

    Journal of ethnopharmacology

    2024  Volume 326, Page(s) 117901

    Abstract: Ethnopharmacological relevance: Wuji Wan (WJW) is a traditional Chinese medicine formula ...

    Abstract Ethnopharmacological relevance: Wuji Wan (WJW) is a traditional Chinese medicine formula that can be found in the "Prescriptions of Taiping Benevolent Dispensary" that has been employed in treating gastric discomfort, burning epigastric pain, and gastric reflux for hundreds of years and has shown promise for treating gastric ulcers (GUs). However, the active components and mechanism of action against GUs remain unclear.
    Aim of the study: The aim of this study was to explore the active components of WJW and elucidate the underlying mechanism involved in treating GUs.
    Materials and methods: Initially, cell viability was measured by a cell counting kit 8 (CCK-8) assay to evaluate the efficacy of WJW-containing serum in vitro. The gastric ulcer index, ulcer inhibition rate, hematoxylin and staining (H&E), and periodic acid-Schiff (PAS) staining were used to evaluate the therapeutic effect of WJW in vivo. Subsequently, the levels of inflammatory factors and oxidative stress factors were determined using an enzyme-linked immunosorbent assays (ELISA) on in vitro and in vivo samples. Additionally, UPLC-Q Exactive Plus Orbitrap HRMS was used to analyze the components that were absorbed into the blood of WJW and its metabolites. Network pharmacology and metabolomics were subsequently used to identify the targets and pathways. Real-time quantitative PCR (RT‒qPCR) and Western blotting were used to verify the mRNA and protein levels of the key targets and pathways. Finally, the active components were identified by molecular docking to verify the binding stability of the components and key targets.
    Results: WJW-containing serum ameliorated ethanol-induced damage in GES-1 cells and promoted cell healing. WJW-containing serum reduced IL-6, TNF-α, MDA, and LDH levels while increasing IL-10, SOD, and T-AOC levels in the cells. Moreover, WJW treatment resulted in decreased IL-6, TNF-α, and MDA levels and increased IL-10, SOD, PGE
    Conclusions: WJW treatment reduces inflammation and oxidative stress injury and inhibits apoptosis signaling pathways. The main active components are berberine, palmatine, coptisine, evodiamine, rutaecarpine, evocarpine, and paeoniflorin. In this paper, we provide a new strategy for exploring the active components of traditional Chinese medicine formulas for the treatment of diseases based on target mechanisms.
    MeSH term(s) Animals ; Rats ; Stomach Ulcer/chemically induced ; Stomach Ulcer/drug therapy ; Caspase 3 ; Caspase 9 ; Interleukin-10 ; Berberine ; Cyclooxygenase 2 ; Interleukin-6 ; Molecular Docking Simulation ; Network Pharmacology ; Tumor Necrosis Factor-alpha ; Superoxide Dismutase ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Glucosides ; Monoterpenes
    Chemical Substances peoniflorin (21AIQ4EV64) ; Caspase 3 (EC 3.4.22.-) ; Caspase 9 (EC 3.4.22.-) ; Interleukin-10 (130068-27-8) ; Berberine (0I8Y3P32UF) ; Cyclooxygenase 2 (EC 1.14.99.1) ; Interleukin-6 ; Tumor Necrosis Factor-alpha ; Superoxide Dismutase (EC 1.15.1.1) ; Drugs, Chinese Herbal ; Glucosides ; Monoterpenes
    Language English
    Publishing date 2024-02-08
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2024.117901
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Shen Qi Wan-Containing Serum Alleviates Renal Interstitial Fibrosis via Restraining Notch1-Mediated Epithelial-Mesenchymal Transition.

    Chen, Hongshu / Zhou, Xiaojie / Yang, Yuanxiao / Feng, Yaorong

    Evidence-based complementary and alternative medicine : eCAM

    2023  Volume 2023, Page(s) 3352353

    Abstract: Objective: Shen Qi Wan (SQW) is the most classic prescription for the clinical therapy ...

    Abstract Objective: Shen Qi Wan (SQW) is the most classic prescription for the clinical therapy of chronic kidney disease in China. Nevertheless, the function of SQW in renal interstitial fibrosis (RIF) has not been clearly clarified. Our purpose was to explore the protective function of SQW on RIF.
    Methods: After intervention with SQW-containing serum alone at increasing concentrations (2.5, 5, and 10%) or in combination with siNotch1, the transforming growth factor-beta (TGF-
    Results: SQW-containing serum intensified the viability of TGF-
    Conclusion: Collectively, these findings elucidated that SQW-containing serum attenuated RIF via restraining EMT through the repression of the Notch1 pathway.
    Language English
    Publishing date 2023-02-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2023/3352353
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  5. Article ; Online: Angong Niuhuang Wan inhibit ferroptosis on ischemic and hemorrhagic stroke by activating PPARγ/AKT/GPX4 pathway.

    Bai, Xue / Zheng, Enqi / Tong, Lin / Liu, Yang / Li, Xianyu / Yang, Hong / Jiang, Jie / Chang, Zhenghui / Yang, Hongjun

    Journal of ethnopharmacology

    2023  Volume 321, Page(s) 117438

    Abstract: Ethnopharmacological relevance: Angong Niuhuang Wan (AGNHW) is a prescription ...

    Abstract Ethnopharmacological relevance: Angong Niuhuang Wan (AGNHW) is a prescription from traditional Chinese medicine (TCM) that has been used for centuries to treat ischemic stroke (IS) and hemorrhagic stroke (HS). According to a recent study, targeting ferroptosis might be effective in the management of IS and HS. However, the ferroptosis-related effects and mechanisms of AGNHW have not yet been reported.
    Aim of the study: This research examines the anti-ferroptosis mechanisms of AGNHW in the treatment of IS and HS.
    Materials and methods: A system pharmacological approach including in vivo experiment, UHPLC-Q-Orbitrap HRMS, network pharmacology, molecular docking, microscale thermophoresis, and in vitro experiment was utilized to study the anti-ferroptosis mechanisms of AGNHW against IS and HS.
    Results: In vivo experiments indicated that AGNHW enhanced nerve function, decreased cerebral infarct volume, ameliorated histological brain injuries, improved the structural integrity of the blood-brain barrier, ameliorated the mitochondrial dysfunction and morphology disruption, and inhibits ROS, LPO and Fe
    Conclusions: AGNHW attenuated ferroptosis in treating IS and HS by targeting the PPARγ/AKT/GPX4 pathway. This work reveals AGNHW's anti-ferroptosis mechanism against IS and HS, but it also develops an integrated approach to demonstrate the common characteristics of drugs in treating different diseases.
    MeSH term(s) Animals ; Rats ; Hemorrhagic Stroke ; PPAR gamma ; Proto-Oncogene Proteins c-akt ; Ferroptosis ; Molecular Docking Simulation ; Phosphatidylinositol 3-Kinases ; Ischemic Stroke/drug therapy
    Chemical Substances niuhuang (4162LZ0N4O) ; PPAR gamma ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-)
    Language English
    Publishing date 2023-11-18
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2023.117438
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  6. Article ; Online: Zuo Gui Wan Promotes Osteogenesis via PI3K/AKT Signaling Pathway: Network Pharmacology Analysis and Experimental Validation.

    Yang, Shuo / Zhang, Bin / Wang, Yu-Guo / Liu, Zi-Wei / Qiao, Bo / Xu, Juan / Zhao, Li-Sheng

    Current medical science

    2023  Volume 43, Issue 5, Page(s) 1051–1060

    Abstract: Objective: Osteogenesis is vitally important for bone defect repair, and Zuo Gui Wan (ZGW) is ...

    Abstract Objective: Osteogenesis is vitally important for bone defect repair, and Zuo Gui Wan (ZGW) is a classic prescription in traditional Chinese medicine (TCM) for strengthening bones. However, the specific mechanism by which ZGW regulates osteogenesis is still unclear. The current study is based on a network pharmacology analysis to explore the potential mechanism of ZGW in promoting osteogenesis.
    Methods: A network pharmacology analysis followed by experimental validation was applied to explore the potential mechanisms of ZGW in promoting the osteogenesis of bone marrow mesenchymal stem cells (BMSCs).
    Results: In total, 487 no-repeat targets corresponding to the bioactive components of ZGW were screened, and 175 target genes in the intersection of ZGW and osteogenesis were obtained. And 28 core target genes were then obtained from a PPI network analysis. A GO functional enrichment analysis showed that the relevant biological processes mainly involve the cellular response to chemical stress, metal ions, and lipopolysaccharide. Additionally, KEGG pathway enrichment analysis revealed that multiple signaling pathways, including the phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) signaling pathway, were associated with ZGW-promoted osteogensis. Further experimental validation showed that ZGW could increase alkaline phosphatase (ALP) activity as well as the mRNA and protein levels of ALP, osteocalcin (OCN), and runt related transcription factor 2 (Runx 2). What's more, Western blot analysis results showed that ZGW significantly increased the protein levels of p-PI3K and p-AKT, and the increases of these protein levels significantly receded after the addition of the PI3K inhibitor LY294002. Finally, the upregulated osteogenic-related indicators were also suppressed by the addition of LY294002.
    Conclusion: ZGW promotes the osteogenesis of BMSCs via PI3K/AKT signaling pathway.
    MeSH term(s) Proto-Oncogene Proteins c-akt/metabolism ; Phosphatidylinositol 3-Kinases/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Osteogenesis ; Network Pharmacology ; Cell Differentiation ; Signal Transduction
    Chemical Substances Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; zuo gui wan ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-)
    Language English
    Publishing date 2023-10-09
    Publishing country China
    Document type Journal Article
    ZDB-ID 2931065-9
    ISSN 2523-899X ; 2096-5230
    ISSN (online) 2523-899X
    ISSN 2096-5230
    DOI 10.1007/s11596-023-2782-x
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  7. Article ; Online: Uncovering the pharmacological mechanism of Shou Tai Wan on recurrent spontaneous abortion: A integrated pharmacology strategy-based research.

    Yang, Kailin / Zeng, Liuting / Li, Yuwei / Wu, Lingyu / Xiang, Wang / Wu, Xiaolan / Wang, Guiyun / Bao, Tingting / Huang, Shanshan / Yu, Rong / Zhang, Guomin / Liu, Huiping

    Journal of ethnopharmacology

    2023  Volume 323, Page(s) 117589

    Abstract: Ethnopharmacological relevance: Shou Tai Wan (STW), a traditional Chinese medicine formula, has ...

    Abstract Ethnopharmacological relevance: Shou Tai Wan (STW), a traditional Chinese medicine formula, has been historically used for the treatment of recurrent spontaneous abortion (RSA). Despite its long-standing usage, the exact mechanism underlying the therapeutic effects of STW remains unclear in the existing literature.
    Aims of this study: To explore the Pharmacological Mechanism of STW on RSA.
    Methods: A network pharmacological methodology was utilized to predict the active compounds and potential targets of STW, collect the RSA targets and other human proteins of STW, and analyze the STW related networks. The animal experiments were also performed to validate the effect of STW on RSA.
    Results: The results of network analysis showed that STW may regulate PI3K/AKT, MAPK, FoxO signaling pathways and so on. Animal experiment established the RSA model with CBA/J × DBA/2 mice. It was found that STW can reduce the embryo absorption rate of RSA group (p < 0.05) and balance the expression of Th 1/Th2 type cytokines compared with the model group. After 14 days of administration, the decidual and placental tissues were taken and the CD4
    Conclusion: STW may achieve therapeutic effects by interfering with the signaling pathways, biological processes and targets discovered in this study. It provides a new perspective for revealing the immunological mechanism of STW in the treatment of RSA, and also provides a theoretical basis for the clinical use of STW in the treatment of RSA.
    MeSH term(s) Mice ; Animals ; Pregnancy ; Female ; Humans ; Phosphatidylinositol 3-Kinases ; Placenta ; Mice, Inbred DBA ; Mice, Inbred CBA ; Abortion, Habitual/drug therapy
    Chemical Substances Phosphatidylinositol 3-Kinases (EC 2.7.1.-)
    Language English
    Publishing date 2023-12-15
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2023.117589
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  8. Article: Immunomodulatory effects of modified Liu-Wei-Di-Huang-Wan Traditional Chinese medicine on allergic asthmatic mice.

    Tsai, Jaw-Ji / Yen, Chung-Yang / Hsu, Chun-Hsien / Yu, Sheng-Jie / Chen, Chao-Hsien / Liao, En-Chih

    Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology

    2023  Volume 19, Issue 1, Page(s) 35

    Abstract: ... patients. Few studies evaluate the ameliorating effects of modified Liu-Wei-Di-Huang-Wan (modified LWDHW ...

    Abstract Background: Allergic asthma occurs worldwide and is particularly prevalent in westernized countries characterized by chronic airway inflammation resulting in airway hyperresponsiveness. The house dust mites (HDM) including Dermatophagoides pteronyssinus are major sources of sensitization and triggering allergic symptoms in asthmatic patients. The Der p 2 is a major allergen and the predominant source of causative respiratory disorders which induce airway inflammation and bronchial constriction in mite-allergic patients. Few studies evaluate the ameliorating effects of modified Liu-Wei-Di-Huang-Wan (modified LWDHW) on allergic asthma.
    Methods: This study aimed to investigate the immunological mechanisms of modified LWDHW on the reductions of airway inflammation, signal transduction, inflammatory cytokine production, Th2 cell proliferation, and bronchial obstruction in Der p 2-induced asthmatic mice.
    Results: At least ten active ingredients were contained in the formula of modified LWDHW- 1217A and 1217B. Results showed that the immunoglobulin generations (Der p 2 specific- IgE and IgG1), inflammatory cytokine productions (IL-5 and IL-13) in the Sera and BALF could be down-regulated, and the Th1-cytokine productions (IL-12 and IFN-γ) be increased after immunotherapy with modified LWDHW of 1217A or 1217B. The inflammatory cell infiltrations (macrophages, eosinophils, and neutrophils) in the airway and the expressions of T
    Conclusion: It revealed that 1217A or 1217B could regulate the immune responses and improve pulmonary function. Data suggests that modified LWDHW of 1217A or 1217B have the potential for use as a therapeutic intervention for the treatment of mite allergen Der p 2-induced allergic asthma.
    Language English
    Publishing date 2023-04-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2434973-2
    ISSN 1710-1492 ; 1710-1484
    ISSN (online) 1710-1492
    ISSN 1710-1484
    DOI 10.1186/s13223-023-00792-5
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  9. Article ; Online: Huanglian Jiedu Wan intervened with "Shi-Re Shanghuo" syndrome through regulating immune balance mediated by biomarker succinate.

    Luo, Keke / Zhao, Haiyu / Wang, Mengxiao / Tian, Mengyao / Si, Nan / Xia, Wen / Song, Jianfang / Chen, Yunqin / Wang, Linna / Zhang, Yan / Wei, Xiaolu / Li, Xing / Qin, Guangyuan / Yang, Jiaying / Wang, Hongjie / Bian, Baolin / Zhou, Yanyan

    Clinical immunology (Orlando, Fla.)

    2023  Volume 258, Page(s) 109861

    Abstract: ... syndrome was gradually universal. "Huanglian Jiedu Wan" (HLJDW) was the first new syndrome Chinese medicine ...

    Abstract With increasing stress in daily life and work, subhealth conditions induced by "Shi-Re Shanghuo" syndrome was gradually universal. "Huanglian Jiedu Wan" (HLJDW) was the first new syndrome Chinese medicine approved for the treatment of "Shi-Re Shanghuo" with promising clinical efficacy. Preliminary small-sample clinical studies have identified some notable biomarkers (succinate, 4-hydroxynonenal, etc.). However, the correlation and underlying mechanism between these biomarkers of HLJDW intervention on "Shi-Re Shanghuo" syndrome remained ambiguous. Therefore, this study was designed as a randomized, double-blind, multicenter, placebo-controlled Phase II clinical trial, employing integrated analysis techniques such as non-targeted and targeted metabolomics, salivary microbiota, proteomics, parallel peaction monitoring, molecular docking and surface plasmon resonance (SPR). The results of the correlation analysis indicated that HLJDW could mediate the balance between inflammation and immunity through succinate produced via host and microbial source to intervene "Shi-Re Shanghuo" syndrome. Further through the HIF1α/MMP9 pathway, succinate regulated downstream arachidonic acid metabolism, particularly the lipid peroxidation product 4-hydroxynonenal. Finally, an animal model of recurrent oral ulcers induced by "Shi-Re Shang Huo" was established and HLJDW was used for intervention, key essential indicators (succinate, glutamine, 4-hydroxynonenal, arachidonic acid metabolism) essential in the potential pathway HIF1α/MMP9 discovered in clinical practice were validated. The results were found to be consistent with our clinical findings. Taken together, succinate was observed as an important signal that triggered immune responses, which might serve as a key regulatory metabolic switch or marker of "Shi-Re Shanghuo" syndrome treated with HLJDW.
    MeSH term(s) Animals ; Arachidonic Acid ; Biomarkers ; Drugs, Chinese Herbal ; Matrix Metalloproteinase 9 ; Molecular Docking Simulation ; Succinates/therapeutic use ; Succinic Acid ; Humans
    Chemical Substances 4-hydroxy-2-nonenal (K1CVM13F96) ; Arachidonic Acid (27YG812J1I) ; Biomarkers ; Drugs, Chinese Herbal ; huanglian-jie-du decoction ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Succinates ; Succinic Acid (AB6MNQ6J6L)
    Language English
    Publishing date 2023-12-06
    Publishing country United States
    Document type Clinical Trial, Phase II ; Journal Article ; Multicenter Study ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 1459903-x
    ISSN 1521-7035 ; 1521-6616
    ISSN (online) 1521-7035
    ISSN 1521-6616
    DOI 10.1016/j.clim.2023.109861
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  10. Article ; Online: RNA-seq combined network pharmacology reveals that Fu-Gan-Wan (FGW) inhibits liver fibrosis via NF-κB/CCL2/CCR2 and lipid peroxidation via Nrf2/HMOX1 signaling pathway.

    Shi, Hanlin / Duan, Xiaohong / Dong, Jingcheng / Tao, Yanyan / Lei, Yang

    Journal of ethnopharmacology

    2024  Volume 326, Page(s) 117963

    Abstract: ... characterized by excessive collagen deposition, without effective therapeutic agents in the clinic. Fu-Gan-Wan ...

    Abstract Ethnopharmacological relevance: Liver fibrosis is a serious complication of liver disease characterized by excessive collagen deposition, without effective therapeutic agents in the clinic. Fu-Gan-Wan (FGW) is an empirical formula used for the clinical treatment of hepatitis and cirrhosis. It has been shown to reverse experimental liver fibrosis. However, its corresponding mechanisms remain unclear.
    Aim of the review: This study aimed to elucidate the key pathways and target genes of FGW in attenuating liver fibrosis.
    Materials and methods: The therapeutic effects of different doses of FGW on liver fibrosis were investigated using a 2 mL/kg 15% CCl
    Results: We found that 19.5 g/kg FGW significantly down-regulated CCl
    Conclusions: This study demonstrated that FGW exhibits potential in mitigating CCl
    MeSH term(s) Mice ; Animals ; NF-kappa B/metabolism ; Transforming Growth Factor beta1/metabolism ; NF-E2-Related Factor 2/genetics ; NF-E2-Related Factor 2/metabolism ; Lipid Peroxidation ; Network Pharmacology ; RNA-Seq ; Liver Cirrhosis/chemically induced ; Liver Cirrhosis/drug therapy ; Liver Cirrhosis/metabolism ; Signal Transduction ; Liver ; Collagen/metabolism ; Carbon Tetrachloride/pharmacology ; Hepatic Stellate Cells
    Chemical Substances NF-kappa B ; Transforming Growth Factor beta1 ; NF-E2-Related Factor 2 ; Collagen (9007-34-5) ; Carbon Tetrachloride (CL2T97X0V0)
    Language English
    Publishing date 2024-02-20
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2024.117963
    Database MEDical Literature Analysis and Retrieval System OnLINE

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