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Article: A Set of Diagnostic Tests for Detection of Active

Chand, Meenal / Vydyam, Pratap / Pal, Anasuya C / Thekkiniath, Jose / Darif, Dounia / Li, Zeng / Choi, Jae-Yeon / Magni, Ruben / Luchini, Alessandra / Tonnetti, Laura / Horn, Elizabeth J / Tufts, Danielle M / Ben Mamoun, Choukri

medRxiv : the preprint server for health sciences

2024

Abstract: Human babesiosis is a rapidly emerging and potentially fatal tick-borne disease caused by intraerythrocytic apicomplexan parasites of the : Short summary: We developed two ELISA-based assays, BdACA38 and BdACA234, for ... ...

Abstract	Human babesiosis is a rapidly emerging and potentially fatal tick-borne disease caused by intraerythrocytic apicomplexan parasites of the Short summary: We developed two ELISA-based assays, BdACA38 and BdACA234, for detecting
Language	English
Publishing date	2024-03-26
Publishing country	United States
Document type	Preprint
DOI	10.1101/2024.03.25.24304816
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Article ; Online: Type I and III interferons are good markers to monitor COVID-19 pathophysiology.

Darif, Dounia / Ejghal, Rajaâ / Desterke, Christophe / Outlioua, Ahmed / Hammi, Ikram / Lemrani, Meryem / Hilali, Farida / Guessous, Fadila / Zaid, Younes / Akarid, Khadija

Cytokine

2023 Volume 165, Page(s) 156172

Abstract: The COVID-19 pandemic has caused millions of deaths and has resulted in disastrous societal and economic impacts worldwide. During SARS-CoV-2 infection, abnormal levels of pro-inflammatory cytokines have been observed and were associated to the severity ... ...

Abstract	The COVID-19 pandemic has caused millions of deaths and has resulted in disastrous societal and economic impacts worldwide. During SARS-CoV-2 infection, abnormal levels of pro-inflammatory cytokines have been observed and were associated to the severity of the disease. Type I (-α/β) and Type III (IFN-λ) interferons are family members of cytokines that play an important role in fighting viral replication during the early phases of infection. The location and timing of the IFNs production have been shown to be decisive for the COVID-19 outcome. Despite the effectiveness of COVID-19 vaccines and with the emergence of new SARS-CoV-2 variants, a better understanding of the involvement of IFNs as players in antiviral immunity in the COVID-19 pathophysiology is necessary to implement additional potent prophylactic and/or therapeutic approaches. In this study, we investigated the role of type I and III IFN in COVID-19 pathophysiology. We first analyzed the IFN-α, IFN-β and IFN- λ mRNA expression in nasopharyngeal swabs and blood samples from Moroccan patients infected with SARS-CoV-2 and secondly correlated these IFNs expressions with COVID-19 clinical and biological parameters. Our results showed that in the upper airways of patients with mild, non-severe, or severe COVID-19 manifestations, the IFN- α, - β and - λ are expressed in the same manner as in controls. However, in blood samples their expression was downregulated in all groups. Univariate linear models with interferons as predictors to evaluate clinical-biological parameters highlighted that the main clinical-biological relations were found when testing: FiO2, Lymphocyte values and virus load. Furthermore, the multivariate models confirmed that quantifications of interferons during COVID-19 are good biological markers for tracking COVID-19 pathophysiology.
MeSH term(s)	Humans ; Interferons ; COVID-19 ; COVID-19 Vaccines ; Pandemics ; SARS-CoV-2 ; Antiviral Agents ; Cytokines ; Interferon-alpha ; Interferon Lambda ; Interferon Type I
Chemical Substances	Interferons (9008-11-1) ; COVID-19 Vaccines ; Antiviral Agents ; Cytokines ; Interferon-alpha ; Interferon Lambda ; Interferon Type I
Language	English
Publishing date	2023-03-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1018055-2
ISSN	1096-0023 ; 1043-4666
ISSN (online)	1096-0023
ISSN	1043-4666
DOI	10.1016/j.cyto.2023.156172
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Article: The Modulation of NADPH Oxidase Activity in Human Neutrophils by Moroccan Strains of

Maksouri, Hasnaa / Darif, Dounia / Estaquier, Jerome / Riyad, Myriam / Desterke, Christophe / Lemrani, Meryem / Dang, Pham My-Chan / Akarid, Khadija

Microorganisms

2021 Volume 9, Issue 5

Abstract: Polymorphonuclear neutrophils (PMNs) are the first phagocyte recruited and infected ... ...

Abstract	Polymorphonuclear neutrophils (PMNs) are the first phagocyte recruited and infected by
Language	English
Publishing date	2021-05-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9051025
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Article ; Online: The pro-inflammatory cytokines in COVID-19 pathogenesis: What goes wrong?

Darif, Dounia / Hammi, Ikram / Kihel, Ayyoub / El Idrissi Saik, Imane / Guessous, Fadila / Akarid, Khadija

Microbial pathogenesis

2021 Volume 153, Page(s) 104799

Abstract: The outbreak of coronavirus disease 2019 (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection, has emerged in China in December 2019 and rapidly spread to more than 196 countries worldwide. The physiopathology of ... ...

Abstract	The outbreak of coronavirus disease 2019 (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection, has emerged in China in December 2019 and rapidly spread to more than 196 countries worldwide. The physiopathology of human SARS-CoV-2 has not been completely understood, but its pathogenesis has been linked to a disproportionate response of the immune system. Just as described for SARS and MERS, an uncontrolled systemic inflammatory response, known as cytokine release syndrome (CRS) was observed in severe COVID-19 patients. It results from the release by immune and non-immune effector cells of substantial amounts of pro-inflammatory cytokines and appears to contribute to SARS-CoV-2 pulmonary inflammation and extensive lung damage. In addition, hyper-coagulation and thrombosis resulted from the important release of pro-inflammatory cytokines contribute to the lethality of subjects severely infected with SARS-CoV-2. It is therefore essential to have a deep understanding of the various cytokines involved in this exacerbated immune response, and that could be targeted by potential immunological treatments. The aim of this review was to gather the current knowledge about the role of pro-inflammatory cytokines, namely IL-1β, IL-6, IL-8, IL-17 and TNFα in SARS-CoV-2 CRS, the probable causes and clinical outcomes of this phenomenon in severe cases of COVID-19.
MeSH term(s)	COVID-19/pathology ; Cytokine Release Syndrome/pathology ; Cytokines/blood ; Cytokines/immunology ; Humans ; Macrophage Activation/immunology ; SARS-CoV-2/immunology ; Thrombosis/pathology
Chemical Substances	Cytokines
Language	English
Publishing date	2021-02-18
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2021.104799
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Article ; Online: Capparis spinosa inhibits Leishmania major growth through nitric oxide production in vitro and arginase inhibition in silico.

Darif, Dounia / Nait Irahal, Imane / Hammi, Ikram / Kihel, Ayyoub / Kachmar, Mohamed Reda / Riyad, Myriam / Hmimid, Fouzia / Akarid, Khadija

Experimental parasitology

2022 Volume 245, Page(s) 108452

Abstract: Cutaneous leishmaniasis is an infectious disease, considered as a major public health problem in different regions of the world. The current treatments are limited due to their toxicity and treatment failures, which have increased the search for new ... ...

Abstract	Cutaneous leishmaniasis is an infectious disease, considered as a major public health problem in different regions of the world. The current treatments are limited due to their toxicity and treatment failures, which have increased the search for new substances of natural origin to control this infection. Capparis spinosa is an important medicinal plant, rich in biochemical compounds with a broad range of activities including antimicrobial effects. Nevertheless, more investigations are still needed to determine its effect on Leishmania parasites. This study aimed to evaluate the effect of C. spinosa' extracts on Leishmania major promastigotes and amastigotes growth as well as on L-arginine metabolic pathways, especially the production of leishmanicidal molecules such as nitric oxide. Our results showed that C. spinosa' methanolic and aqueous extracts contained polyphenols and flavonoids at different concentrations. The methanolic extract of C. spinosa, compared to the aqueous extract, showed significantly higher amounts of total polyphenols (21.23 ± 1.08) mg GAE/g of dw (P < 0.05), as well as a higher antioxidant activity evaluated respectively by Reducing Power and DPPH (EC
MeSH term(s)	Leishmania major ; Nitric Oxide/metabolism ; Arginase/metabolism ; Capparis/chemistry ; Capparis/metabolism ; Flavonoids/pharmacology ; Polyphenols/pharmacology ; Plant Extracts/pharmacology ; Methanol/pharmacology
Chemical Substances	Nitric Oxide (31C4KY9ESH) ; Arginase (EC 3.5.3.1) ; Flavonoids ; Polyphenols ; Plant Extracts ; Methanol (Y4S76JWI15)
Language	English
Publishing date	2022-12-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	391089-1
ISSN	1090-2449 ; 0014-4894
ISSN (online)	1090-2449
ISSN	0014-4894
DOI	10.1016/j.exppara.2022.108452
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Article ; Online: Therapeutic Potential of Clove Essential Oil in Diabetes: Modulation of Pro-Inflammatory Mediators, Oxidative Stress and Metabolic Enzyme Activities.

Nait Irahal, Imane / Darif, Dounia / Guenaou, Ismail / Hmimid, Fouzia / Azzahra Lahlou, Fatima / Ez-Zahra Ousaid, Fatima / Abdou-Allah, Fatima / Aitsi, Lamiaa / Akarid, Khadija / Bourhim, Noureddine

Chemistry & biodiversity

2023 Volume 20, Issue 3, Page(s) e202201169

Abstract: Type 1 diabetes is characterized by insulin deficiency due to the destruction of pancreatic β cells, leading to hyperglycemia, which in turn induces vascular complications. In the current study, we investigated the effect of intraperitoneal ... ...

Abstract	Type 1 diabetes is characterized by insulin deficiency due to the destruction of pancreatic β cells, leading to hyperglycemia, which in turn induces vascular complications. In the current study, we investigated the effect of intraperitoneal administration of clove essential oil (CEO: 20 mg/kg body weight) on certain oxidative stress and glucose metabolism enzymes, as well as the expression of proinflammatory mediators. Administration of CEO to diabetic rats showed a significant decline in blood glucose levels, total cholesterol, and xanthine oxidase, compared to the streptozotocin group. Furthermore, these treated rats elicited a notable attenuation in the levels of lipid peroxides, and thiols groups in both liver and brain tissues. The activities of antioxidant and metabolic enzymes were reverted to normality in diabetic upon CEO administration. In addition to its protective effects on red blood cell hemolysis, CEO is a potent α-amylase inhibitor with an IC
MeSH term(s)	Rats ; Animals ; Syzygium ; Clove Oil/pharmacology ; Clove Oil/therapeutic use ; Oils, Volatile/pharmacology ; Oils, Volatile/therapeutic use ; Diabetes Mellitus, Experimental/chemically induced ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/metabolism ; Inflammation Mediators/metabolism ; Inflammation Mediators/pharmacology ; Oxidative Stress ; Antioxidants/metabolism ; Streptozocin ; Hypoglycemic Agents/pharmacology
Chemical Substances	Clove Oil ; Oils, Volatile ; Inflammation Mediators ; Antioxidants ; Streptozocin (5W494URQ81) ; Hypoglycemic Agents
Language	English
Publishing date	2023-02-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2139001-0
ISSN	1612-1880 ; 1612-1872
ISSN (online)	1612-1880
ISSN	1612-1872
DOI	10.1002/cbdv.202201169
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Article ; Online: The different faces of the NLRP3 inflammasome in cutaneous Leishmaniasis: A review.

Kihel, Ayyoub / Hammi, Ikram / Darif, Dounia / Lemrani, Meryem / Riyad, Myriam / Guessous, Fadila / Akarid, Khadija

Cytokine

2020 Volume 147, Page(s) 155248

Abstract: Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease caused by Protozoa of the genus Leishmania. Clinical manifestations of this disease are the result of a complex interplay of diverse factors, including the genetic background and the immune ...

Abstract	Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease caused by Protozoa of the genus Leishmania. Clinical manifestations of this disease are the result of a complex interplay of diverse factors, including the genetic background and the immune status of the host. Understanding the impact of these factors on the CL pathology may provide new targets to manage the infection and improve clinical outcome. The NLRP3 inflammasome, an innate immune complex of several cell types, seems to be involved in the CL physiopathology. Current studies of its role show contradictory effects of this complex on the evolution of Leishmania infection in mice and humans. In this review, we discuss the data regarding different roles of the NLRP3 inflammasome in murine and human CL.
MeSH term(s)	Animals ; Humans ; Inflammasomes/metabolism ; Leishmania/pathogenicity ; Leishmaniasis, Cutaneous/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
Chemical Substances	Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein
Language	English
Publishing date	2020-08-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1018055-2
ISSN	1096-0023 ; 1043-4666
ISSN (online)	1096-0023
ISSN	1043-4666
DOI	10.1016/j.cyto.2020.155248
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Article ; Online: The Modulation of NADPH Oxidase Activity in Human Neutrophils by Moroccan Strains of Leishmania major and Leishmania tropica Is Not Associated with p47 phox Phosphorylation

Hasnaa Maksouri / Dounia Darif / Jerome Estaquier / Myriam Riyad / Christophe Desterke / Meryem Lemrani / Pham My-Chan Dang / Khadija Akarid

Microorganisms, Vol 9, Iss 1025, p

2021 Volume 1025

Abstract: Polymorphonuclear neutrophils (PMNs) are the first phagocyte recruited and infected by Leishmania . They synthetize superoxide anions (O 2 − ) under the control of the NADPH oxidase complex. In Morocco, Leishmania major and L. tropica are the main ... ...

Abstract	Polymorphonuclear neutrophils (PMNs) are the first phagocyte recruited and infected by Leishmania . They synthetize superoxide anions (O 2 − ) under the control of the NADPH oxidase complex. In Morocco, Leishmania major and L. tropica are the main species responsible for cutaneous leishmaniasis (CL). The impact of these parasites on human PMN functions is still unclear. We evaluated the in vitro capacity of primary Moroccan strains of L. major and L. tropica to modulate PMN O 2 − production and p47 phox phosphorylation status of the NADPH oxidase complex. PMNs were isolated from healthy blood donors, and their infection rate was measured by microscopy. O 2 − production was measured by superoxide dismutase–inhibitable reduction of cytochrome C. P47 phox phosphorylation was analyzed by Western blot using specific antibodies against Ser328 and Ser345 sites. Whereas we did not observe any difference in PMN infectivity rate, our results indicated that only L. tropica promastigotes inhibited both fMLF- and PMA-mediated O 2 − production independently of p47 phox phosphorylation. Leishmania soluble antigens (SLAs) from both species significantly inhibited O 2 − induced by fMLF or PMA. However, they only decreased PMA-induced p47phox phosphorylation. L. major and L. tropica modulated differently O 2 − production by human PMNs independently of p47 phox phosphorylation. The inhibition of ROS production by L. tropica could be a mechanism of its survival within PMNs that might explain the reported chronic pathogenicity of L. tropica CL.
Keywords	Leishmania ; neutrophils ; NADPH oxidase ; ROS ; p47 phox ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: The Modulation of NADPH Oxidase Activity in Human Neutrophils by Moroccan Strains of Leishmania major and Leishmania tropica Is Not Associated with p47phox Phosphorylation

Maksouri, Hasnaa / Darif, Dounia / Estaquier, Jerome / Riyad, Myriam / Desterke, Christophe / Lemrani, Meryem / Dang, Pham My-Chan / Akarid, Khadija

Microorganisms. 2021 May 10, v. 9, no. 5

2021

Abstract: Polymorphonuclear neutrophils (PMNs) are the first phagocyte recruited and infected by Leishmania. They synthetize superoxide anions (O₂⁻) under the control of the NADPH oxidase complex. In Morocco, Leishmania major and L. tropica are the main species ... ...

Abstract	Polymorphonuclear neutrophils (PMNs) are the first phagocyte recruited and infected by Leishmania. They synthetize superoxide anions (O₂⁻) under the control of the NADPH oxidase complex. In Morocco, Leishmania major and L. tropica are the main species responsible for cutaneous leishmaniasis (CL). The impact of these parasites on human PMN functions is still unclear. We evaluated the in vitro capacity of primary Moroccan strains of L. major and L. tropica to modulate PMN O₂⁻ production and p47ᵖʰᵒˣ phosphorylation status of the NADPH oxidase complex. PMNs were isolated from healthy blood donors, and their infection rate was measured by microscopy. O₂⁻ production was measured by superoxide dismutase–inhibitable reduction of cytochrome C. P47ᵖʰᵒˣ phosphorylation was analyzed by Western blot using specific antibodies against Ser328 and Ser345 sites. Whereas we did not observe any difference in PMN infectivity rate, our results indicated that only L. tropica promastigotes inhibited both fMLF- and PMA-mediated O₂⁻ production independently of p47ᵖʰᵒˣ phosphorylation. Leishmania soluble antigens (SLAs) from both species significantly inhibited O₂⁻ induced by fMLF or PMA. However, they only decreased PMA-induced p47phox phosphorylation. L. major and L. tropica modulated differently O₂⁻ production by human PMNs independently of p47ᵖʰᵒˣ phosphorylation. The inhibition of ROS production by L. tropica could be a mechanism of its survival within PMNs that might explain the reported chronic pathogenicity of L. tropica CL.
Keywords	Leishmania major ; Leishmania tropica ; NAD(P)H oxidase (H2O2-forming) ; Western blotting ; cutaneous leishmaniasis ; humans ; microscopy ; neutrophils ; pathogenicity ; phosphorylation ; promastigotes ; Morocco
Language	English
Dates of publication	2021-0510
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9051025
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Leishmaniose généralisée de l'Ancien monde : Premier cas marocain chez un adulte immunocompétent ?

Chiheb, Soumiya / Tazi Saoud, Zineb / El Idrissi Saik, Imane / Darif, Dounia / Hali, Fouzia / Fatoiki, Fatima Zahra El / Dahbi, Hayat Skali / Kihel, Ayyoub / Hammi, Ikram / Soussi Abdellaoui, Maha / Riyad, Myriam

Medecine tropicale et sante internationale

2021 Volume 1, Issue 4

Abstract: Background: Post-kala-azar dermal : Case: A 36-year-old-man from Tata in southern Morocco, with a history of visceral : Conclusion: To our knowledge, this is the first case of generalised leishmaniasis suggesting PKDL reported in a Moroccan ... ...

Title translation	Generalized old world leishmaniasis: first Moroccan case in an immunocompetent adult?
Abstract	Background: Post-kala-azar dermal Case: A 36-year-old-man from Tata in southern Morocco, with a history of visceral Conclusion: To our knowledge, this is the first case of generalised leishmaniasis suggesting PKDL reported in a Moroccan immunocompetent adult.
MeSH term(s)	Adult ; Amphotericin B/therapeutic use ; Humans ; Leishmania ; Leishmaniasis, Cutaneous/complications ; Leishmaniasis, Visceral/complications ; Male ; Meglumine Antimoniate/therapeutic use
Chemical Substances	Meglumine Antimoniate (75G4TW236W) ; Amphotericin B (7XU7A7DROE)
Language	French
Publishing date	2021-11-05
Publishing country	France
Document type	Case Reports ; Journal Article
ISSN	2778-2034
ISSN (online)	2778-2034
DOI	10.48327/mtsi.2021.90
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