Article ; Online: Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans.
2021 Volume 34, Issue 6, Page(s) 108684
Abstract: The diversity of circulating human B cells is unknown. We use single-cell RNA sequencing (RNA-seq ... to examine the diversity of both antigen-specific and total B cells in healthy subjects and malaria-exposed ... individuals. This reveals two B cell lineages: a classical lineage of activated and resting memory B cells and ...
| Abstract | The diversity of circulating human B cells is unknown. We use single-cell RNA sequencing (RNA-seq) to examine the diversity of both antigen-specific and total B cells in healthy subjects and malaria-exposed individuals. This reveals two B cell lineages: a classical lineage of activated and resting memory B cells and an alternative lineage, which includes previously described atypical B cells. Although atypical B cells have previously been associated with disease states, the alternative lineage is common in healthy controls, as well as malaria-exposed individuals. We further track Plasmodium-specific B cells after malaria vaccination in naive volunteers. We find that alternative lineage cells are primed after the initial immunization and respond to booster doses. However, alternative lineage cells develop an atypical phenotype with repeated boosts. The data highlight that atypical cells are part of a wider alternative lineage of B cells that are a normal component of healthy immune responses. |
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| MeSH term(s) | Adult ; Antibodies, Protozoan/immunology ; B-Lymphocytes/immunology ; Child ; Child, Preschool ; Female ; Humans ; Malaria/immunology ; Malaria/prevention & control ; Malaria Vaccines/administration & dosage ; Malaria Vaccines/immunology ; Male ; Plasmodium/immunology ; RNA-Seq ; Vaccination |
| Chemical Substances | Antibodies, Protozoan ; Malaria Vaccines |
| Language | English |
| Publishing date | 2021-02-10 |
| Publishing country | United States |
| Document type | Clinical Trial, Phase III ; Journal Article ; Multicenter Study ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2649101-1 |
| ISSN | 2211-1247 ; 2211-1247 |
| ISSN (online) | 2211-1247 |
| ISSN | 2211-1247 |
| DOI | 10.1016/j.celrep.2020.108684 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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