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Article ; Online: Alpha Satellite DNA in Targeted Drug Therapy for Prostate Cancer.

Feliciello, Isidoro / Ugarković, Đurđica

International journal of molecular sciences

2023 Volume 24, Issue 21

Abstract: Prostate cancer is the most common solid cancer in men and, despite the development of many new therapies, metastatic castration-resistant prostate cancer still remains a deadly disease. Therefore, novel concepts for the treatment of metastatic prostate ... ...

Abstract	Prostate cancer is the most common solid cancer in men and, despite the development of many new therapies, metastatic castration-resistant prostate cancer still remains a deadly disease. Therefore, novel concepts for the treatment of metastatic prostate cancer are needed. In our opinion, the role of the non-coding part of the genome, satellite DNA in particular, has been underestimated in relation to diseases such as cancer. Here, we hypothesise that this part of the genome should be considered as a potential target for the development of new drugs. Specifically, we propose a novel concept directed at the possible treatment of metastatic prostate cancer that is mostly based on epigenetics. Namely, metastatic prostate cancer is characterized by the strongly induced transcription of alpha satellite DNA located in pericentromeric heterochromatin and, according to our hypothesis, the stable controlled transcription of satellite DNA might be important in terms of the control of disease development. This can be primarily achieved through the epigenetic regulation of pericentromeric heterochromatin by using specific enzymes as well as their activators/inhibitors that could act as potential anti-prostate cancer drugs. We believe that our concept is innovative and should be considered in the potential treatment of prostate cancer in combination with other more conventional therapies.
MeSH term(s)	Male ; Humans ; DNA, Satellite/genetics ; Heterochromatin/genetics ; Epigenesis, Genetic ; Prostatic Neoplasms/drug therapy ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms, Castration-Resistant/pathology
Chemical Substances	DNA, Satellite ; Heterochromatin
Language	English
Publishing date	2023-10-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms242115585
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Alpha Satellite DNA in Targeted Drug Therapy for Prostate Cancer

Isidoro Feliciello / Đurđica Ugarković

International Journal of Molecular Sciences, Vol 24, Iss 21, p

2023 Volume 15585

Abstract	Prostate cancer is the most common solid cancer in men and, despite the development of many new therapies, metastatic castration-resistant prostate cancer still remains a deadly disease. Therefore, novel concepts for the treatment of metastatic prostate cancer are needed. In our opinion, the role of the non-coding part of the genome, satellite DNA in particular, has been underestimated in relation to diseases such as cancer. Here, we hypothesise that this part of the genome should be considered as a potential target for the development of new drugs. Specifically, we propose a novel concept directed at the possible treatment of metastatic prostate cancer that is mostly based on epigenetics. Namely, metastatic prostate cancer is characterized by the strongly induced transcription of alpha satellite DNA located in pericentromeric heterochromatin and, according to our hypothesis, the stable controlled transcription of satellite DNA might be important in terms of the control of disease development. This can be primarily achieved through the epigenetic regulation of pericentromeric heterochromatin by using specific enzymes as well as their activators/inhibitors that could act as potential anti-prostate cancer drugs. We believe that our concept is innovative and should be considered in the potential treatment of prostate cancer in combination with other more conventional therapies.
Keywords	metastatic prostate cancer ; satellite DNA ; heterochromatin ; epigenetics ; histone modification ; transcription ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	610
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: mRNA vaccines

Isidoro Feliciello / Alfredo Procino

Journal of Biological Research (2021)

Why and how they should be modified

2021

Abstract: Dear editor, The COVID-19 pandemic has stimulated the production of different therapeutic approaches for the resolution of coronavirus infections. On one hand, nanobiomolecules have been proposed as bait material for viruses, 1 on the other hand ... ...

Abstract	Dear editor, The COVID-19 pandemic has stimulated the production of different therapeutic approaches for the resolution of coronavirus infections. On one hand, nanobiomolecules have been proposed as bait material for viruses, 1 on the other hand unconventional messenger RNA vaccines have been produced like SARS-CoV-2 mRNA vaccines (BioNTech / Pfizer BNT162b2 and Moderna mRNA-1273). [.]
Keywords	L1 retrotransposon ; mRNA-vaccines ; mutation ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	PAGEPress Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Satellite DNAs in Health and Disease.

Ugarković, Đurđica / Sermek, Antonio / Ljubić, Sven / Feliciello, Isidoro

Genes

2022 Volume 13, Issue 7

Abstract: Tandemly repeated satellite DNAs are major components of centromeres and pericentromeric heterochromatin which are crucial chromosomal elements responsible for accurate chromosome segregation. Satellite DNAs also contribute to genome evolution and the ... ...

Abstract	Tandemly repeated satellite DNAs are major components of centromeres and pericentromeric heterochromatin which are crucial chromosomal elements responsible for accurate chromosome segregation. Satellite DNAs also contribute to genome evolution and the speciation process and are important for the maintenance of the entire genome inside the nucleus. In addition, there is increasing evidence for active and tightly regulated transcription of satellite DNAs and for the role of their transcripts in diverse processes. In this review, we focus on recent discoveries related to the regulation of satellite DNA expression and the role of their transcripts, either in heterochromatin establishment and centromere function or in gene expression regulation under various biological contexts. We discuss the role of satellite transcripts in the stress response and environmental adaptation as well as consequences of the dysregulation of satellite DNA expression in cancer and their potential use as cancer biomarkers.
MeSH term(s)	Centromere/genetics ; DNA, Satellite/genetics ; Gene Expression Regulation ; Heterochromatin/genetics
Chemical Substances	DNA, Satellite ; Heterochromatin
Language	English
Publishing date	2022-06-26
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes13071154
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Article ; Online: The pulmonary-proteoliposome as a new therapeutic approach for Coronaviruses.

Feliciello, Isidoro / Procino, Alfredo

Human vaccines & immunotherapeutics

2020 Volume 16, Issue 10, Page(s) 2373

Abstract: We are proposingÂ the use of pulmonary-proteoliposome as a new therapeutic approach for Coronaviruses. The designed strategy represents a potential treatment to reduce the overall viral load in the lungs and to help the immune system to successfully ... ...

Abstract	We are proposingÂ the use of pulmonary-proteoliposome as a new therapeutic approach for Coronaviruses. The designed strategy represents a potential treatment to reduce the overall viral load in the lungs and to help the immune system to successfully stave off the infection.
MeSH term(s)	Angiotensin-Converting Enzyme 2 ; Betacoronavirus ; COVID-19 ; Cell Membrane/metabolism ; Coronavirus Infections/prevention & control ; Membranes, Artificial ; Pandemics/prevention & control ; Peptidyl-Dipeptidase A/metabolism ; Pneumonia, Viral/prevention & control ; Proteolipids/metabolism ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/metabolism ; Viral Load
Chemical Substances	Membranes, Artificial ; Proteolipids ; Spike Glycoprotein, Coronavirus ; proteoliposomes ; spike protein, SARS-CoV-2 ; Peptidyl-Dipeptidase A (EC 3.4.15.1) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23)
Keywords	covid19
Language	English
Publishing date	2020-05-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	2664176-8
ISSN	2164-554X ; 2164-5515
ISSN (online)	2164-554X
ISSN	2164-5515
DOI	10.1080/21645515.2020.1758534
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Article ; Online: Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA.

Đermić, Damir / Ljubić, Sven / Matulić, Maja / Procino, Alfredo / Feliciello, Maria Chiara / Ugarković, Đurđica / Feliciello, Isidoro

Scientific reports

2023 Volume 13, Issue 1, Page(s) 11470

Abstract: The procedure illustrated in this paper represents a new method for transcriptome analysis by PCR (Polymerase Chain Reaction), which circumvents the need for elimination of potential DNA contamination. Compared to the existing methodologies, our method ... ...

Abstract	The procedure illustrated in this paper represents a new method for transcriptome analysis by PCR (Polymerase Chain Reaction), which circumvents the need for elimination of potential DNA contamination. Compared to the existing methodologies, our method is more precise, simpler and more reproducible because it preserves the RNA's integrity, does not require materials and/or reagents that are used for elimination of DNA and it also reduces the number of samples that should be set up as negative controls. This novel procedure involves the use of a specifically modified primer during reverse transcription step, which contains mismatched bases, thus producing cDNA molecules that differ from genomic DNA. By using the same modified primer in PCR amplification, only cDNA template is amplified since genomic DNA template is partially heterologous to the primer. In this way, amplification by PCR is unaffected by any potential DNA contamination since it is specific only for the cDNA template. Furthermore, it accurately reflects the initial RNA concentration of the sample, which is prone to changes due to various physical or enzymatic treatments commonly used by the current methodologies for DNA elimination. The method is particularly suitable for quantification of highly repetitive DNA transcripts, such as satellite DNA.
MeSH term(s)	DNA, Complementary/genetics ; Reverse Transcription ; Polymerase Chain Reaction/methods ; DNA/genetics ; RNA/genetics ; Reverse Transcriptase Polymerase Chain Reaction
Chemical Substances	DNA, Complementary ; DNA (9007-49-2) ; RNA (63231-63-0)
Language	English
Publishing date	2023-07-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-38383-4
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Article: Satellite DNAs in Health and Disease

Ugarković, Đurđica / Sermek, Antonio / Ljubić, Sven / Feliciello, Isidoro

Genes. 2022 June 26, v. 13, no. 7

2022

Abstract	Tandemly repeated satellite DNAs are major components of centromeres and pericentromeric heterochromatin which are crucial chromosomal elements responsible for accurate chromosome segregation. Satellite DNAs also contribute to genome evolution and the speciation process and are important for the maintenance of the entire genome inside the nucleus. In addition, there is increasing evidence for active and tightly regulated transcription of satellite DNAs and for the role of their transcripts in diverse processes. In this review, we focus on recent discoveries related to the regulation of satellite DNA expression and the role of their transcripts, either in heterochromatin establishment and centromere function or in gene expression regulation under various biological contexts. We discuss the role of satellite transcripts in the stress response and environmental adaptation as well as consequences of the dysregulation of satellite DNA expression in cancer and their potential use as cancer biomarkers.
Keywords	biomarkers ; centromeres ; chromosome segregation ; evolution ; gene expression regulation ; heterochromatin ; satellite DNA ; satellites ; stress response
Language	English
Dates of publication	2022-0626
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2527218-4
ISSN	2073-4425
ISSN	2073-4425
DOI	10.3390/genes13071154
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Distinct Regulation of the Expression of Satellite DNAs in the Beetle

Sermek, Antonio / Feliciello, Isidoro / Ugarković, Đurđica

International journal of molecular sciences

2020 Volume 22, Issue 1

Abstract: In the flour beetle, ...

Abstract	In the flour beetle,
MeSH term(s)	Animals ; DNA, Satellite/genetics ; Embryo, Nonmammalian/embryology ; Embryo, Nonmammalian/metabolism ; Gene Expression Profiling/methods ; Gene Expression Regulation, Developmental ; Genome, Insect/genetics ; Heat-Shock Response/genetics ; Heterochromatin/genetics ; Tribolium/embryology ; Tribolium/genetics
Chemical Substances	DNA, Satellite ; Heterochromatin
Language	English
Publishing date	2020-12-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22010296
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Article ; Online: Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA

Damir Đermić / Sven Ljubić / Maja Matulić / Alfredo Procino / Maria Chiara Feliciello / Đurđica Ugarković / Isidoro Feliciello

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 8

Abstract: Abstract The procedure illustrated in this paper represents a new method for transcriptome analysis by PCR (Polymerase Chain Reaction), which circumvents the need for elimination of potential DNA contamination. Compared to the existing methodologies, our ...

Abstract	Abstract The procedure illustrated in this paper represents a new method for transcriptome analysis by PCR (Polymerase Chain Reaction), which circumvents the need for elimination of potential DNA contamination. Compared to the existing methodologies, our method is more precise, simpler and more reproducible because it preserves the RNA’s integrity, does not require materials and/or reagents that are used for elimination of DNA and it also reduces the number of samples that should be set up as negative controls. This novel procedure involves the use of a specifically modified primer during reverse transcription step, which contains mismatched bases, thus producing cDNA molecules that differ from genomic DNA. By using the same modified primer in PCR amplification, only cDNA template is amplified since genomic DNA template is partially heterologous to the primer. In this way, amplification by PCR is unaffected by any potential DNA contamination since it is specific only for the cDNA template. Furthermore, it accurately reflects the initial RNA concentration of the sample, which is prone to changes due to various physical or enzymatic treatments commonly used by the current methodologies for DNA elimination. The method is particularly suitable for quantification of highly repetitive DNA transcripts, such as satellite DNA.
Keywords	Medicine ; R ; Science ; Q
Subject code	612
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Distinct Regulation of the Expression of Satellite DNAs in the Beetle Tribolium castaneum

Antonio Sermek / Isidoro Feliciello / Đurđica Ugarković

International Journal of Molecular Sciences, Vol 22, Iss 296, p

2021 Volume 296

Abstract: In the flour beetle, Tribolium castaneum (peri)centromeric heterochromatin is mainly composed of a major satellite DNA TCAST1 interspersed with minor satellites. With the exception of heterochromatin, clustered satellite repeats are found dispersed ... ...

Abstract	In the flour beetle, Tribolium castaneum (peri)centromeric heterochromatin is mainly composed of a major satellite DNA TCAST1 interspersed with minor satellites. With the exception of heterochromatin, clustered satellite repeats are found dispersed within euchromatin. In order to uncover a possible satellite DNA function within the beetle genome, we analysed the expression of the major TCAST1 and a minor TCAST2 satellite during the development and upon heat stress. The results reveal that TCAST1 transcription was strongly induced at specific embryonic stages and upon heat stress, while TCAST2 transcription is stable during both processes. TCAST1 transcripts are processed preferentially into piRNAs during embryogenesis and into siRNAs during later development, contrary to TCAST2 transcripts, which are processed exclusively into piRNAs. In addition, increased TCAST1 expression upon heat stress is accompanied by the enrichment of the silent histone mark H3K9me3 on the major satellite, while the H3K9me3 level at TCAST2 remains unchanged. The transcription of the two satellites is proposed to be affected by the chromatin state: heterochromatin and euchromatin, which are assumed to be the prevalent sources of TCAST1 and TCAST2 transcripts, respectively. In addition, distinct regulation of the expression might be related to diverse roles that major and minor satellite RNAs play during the development and stress response.
Keywords	satellite DNA ; siRNA ; piRNA ; embryogenesis ; heat stress ; heterochromatin ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	572
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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