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  1. Article: Spirometry: how should we order this bedrock of diagnosis and management for Asthma and COPD?

    Dwyer, Terry M / Abraham, George E

    Respiratory care

    2012  Volume 57, Issue 10, Page(s) 1692–1695

    MeSH term(s) Albuterol/pharmacology ; Bronchodilator Agents/pharmacology ; Female ; Forced Expiratory Volume/drug effects ; Humans ; Male ; Vital Capacity/drug effects
    Chemical Substances Bronchodilator Agents ; Albuterol (QF8SVZ843E)
    Language English
    Publishing date 2012-10
    Publishing country United States
    Document type Comment ; Editorial
    ZDB-ID 603252-7
    ISSN 0020-1324 ; 0098-9142
    ISSN 0020-1324 ; 0098-9142
    DOI 10.4187/respcare.02105
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Just what are the mucous secretions of cystic fibrosis?

    Dwyer, Terry M

    American journal of respiratory cell and molecular biology

    2005  Volume 32, Issue 3, Page(s) 248

    MeSH term(s) Cystic Fibrosis/metabolism ; DNA/metabolism ; Humans ; Mucins/metabolism ; Sputum/metabolism
    Chemical Substances Mucins ; DNA (9007-49-2)
    Language English
    Publishing date 2005-03
    Publishing country United States
    Document type Comment ; Letter
    ZDB-ID 1025960-0
    ISSN 1535-4989 ; 1044-1549
    ISSN (online) 1535-4989
    ISSN 1044-1549
    DOI 10.1165/rcmb.F291
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: About long-term oxygen therapy.

    Abraham, George E / Dwyer, Terry M / Bhagat, Rajesh

    Journal of the Mississippi State Medical Association

    2014  Volume 55, Issue 9, Page(s) 291–293

    MeSH term(s) Altitude ; Contraindications ; Humans ; Oxygen Inhalation Therapy/instrumentation ; Oxygen Inhalation Therapy/utilization ; Pulmonary Disease, Chronic Obstructive/epidemiology ; Pulmonary Disease, Chronic Obstructive/therapy ; Quality of Life ; United States/epidemiology
    Language English
    Publishing date 2014-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604042-1
    ISSN 0026-6396
    ISSN 0026-6396
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Top 10 FACTS YOU FACTS YOU SHOULD KNOW About Spirometry.

    Haynes, Demondes / Abraham, George E / Dwyer, Terry M / Petrini, Marcy F

    Journal of the Mississippi State Medical Association

    2015  Volume 56, Issue 4, Page(s) 95–98

    MeSH term(s) Airway Obstruction/diagnosis ; Airway Obstruction/etiology ; Equipment Design ; Humans ; Respiratory Tract Diseases/diagnosis ; Spirometry/instrumentation ; Spirometry/methods
    Language English
    Publishing date 2015-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604042-1
    ISSN 0026-6396
    ISSN 0026-6396
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Deriving National Continued Competency Priorities for Emergency Medical Services Clinicians.

    Terry, Mark / Powell, Jonathan / Gilmore, W Scott / Way, David P / Dwyer, Andrew / Bhanji, Farhan / Panchal, Ashish R

    Prehospital emergency care

    2022  Volume 27, Issue 4, Page(s) 439–448

    Abstract: Objective: Continued competency is poorly defined in emergency medical services (EMS), with no established method for verifying continued competency at a national level. The objective of this project was to refine understanding of continued competency ... ...

    Abstract Objective: Continued competency is poorly defined in emergency medical services (EMS), with no established method for verifying continued competency at a national level. The objective of this project was to refine understanding of continued competency for EMS clinicians in the U.S. and establish priorities for developing competency assessments.
    Methods: A panel of EMS managers, educators, medical directors, and experts in competency assessment, simulation, and certification used a modified Delphi technique to address two questions: "What is the content for continued competency in EMS that should be assessed or verified?" (content) and "How should continued competency of EMS clinicians be demonstrated?" (process). The Delphi process was conducted through electronic conferencing and survey software over a 6-month period. In round one, panelists responded to open-ended prompts and their contributions were analyzed and categorized into themes by independent reviewers. In round two, the panel rated theme importance using five-point Likert-type scales. In round three, the panel ranked their top 10 themes, and in round four, the panel selected the most important themes for each of the two questions through consensus-building discussions. Descriptive statistics and thematic analyses were performed with Excel and STATA 16.
    Results: Fourteen invited experts participated in all Delphi activities. The panel contributed 70 content and 35 process items from the original prompts. Following thematic analysis, these contributions were reduced to 21 and 14 unique themes, respectively. The final top five prioritized themes for content important for continued competency included (1) airway, respiration, and ventilation, (2) patient assessment, (3) pharmacology, (4) pediatrics, and (5) management of time critical disease progressions. The final top five prioritized themes for the processes for continued competency assessment included (1) assessments of evidence-based practice, (2) performance-based assessments, (3) combined knowledge and skill assessments, (4) performance improvement over time, and (5) frequent, short knowledge assessments.
    Conclusion: This modified Delphi process identified priorities for content and assessment, laying the groundwork for EMS continued competency at a national level. These findings can be leveraged by national task forces to develop transparent and consistent guidelines for systems that verify continued competency related to certification, licensure, and local credentialing.
    MeSH term(s) Humans ; Child ; Emergency Medical Services/methods ; Delphi Technique ; Certification ; Consensus ; Surveys and Questionnaires
    Language English
    Publishing date 2022-10-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 1461751-1
    ISSN 1545-0066 ; 1090-3127
    ISSN (online) 1545-0066
    ISSN 1090-3127
    DOI 10.1080/10903127.2022.2120934
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Sampling airway surface liquid: non-volatiles in the exhaled breath condensate.

    Dwyer, Terry M

    Lung

    2004  Volume 182, Issue 4, Page(s) 241–250

    Abstract: Exhaled breath condensate (EBC) samples contain molecules that have no appreciable vapor pressure; such molecules likely derive from droplets of airway fluid. We analyzed EBC gathered from a total of 62 healthy volunteers in order to quantify the volume ... ...

    Abstract Exhaled breath condensate (EBC) samples contain molecules that have no appreciable vapor pressure; such molecules likely derive from droplets of airway fluid. We analyzed EBC gathered from a total of 62 healthy volunteers in order to quantify the volume of airway liquid that was the source of the non-volatiles; saliva was analyzed as a reference secretion. EBC urea averaged 0.52 +/- 0.12 micromol/L (n = 18), an 8,600-fold dilution from predicted blood urea nitrogen levels. Protein averaged 2.3 +/- 0.3 microg/ml (n = 31), three orders of magnitude less than in saliva (1.4 +/- 0.1 mg/ml, n = 15). EBC ammonia was 6.6 +/- 0.6 mmol/L (1/15 that of saliva) and EBC ammonium ion was 0.90 +/- 0.19 micromol/L, concentrations that are incompatible with an 8,600-fold dilution from a biological source. Thus, urea-derived dilution factors may be used to interpret EBC non-volatile molecules, but not EBC volatiles.
    MeSH term(s) Albumins/metabolism ; Ammonia/metabolism ; Breath Tests/methods ; Exhalation/physiology ; Female ; Humans ; Male ; Proteins/metabolism ; Reference Values ; Respiratory Function Tests ; Specimen Handling/methods ; Surface Properties ; Urea/metabolism
    Chemical Substances Albumins ; Proteins ; Ammonia (7664-41-7) ; Urea (8W8T17847W)
    Language English
    Publishing date 2004-10-15
    Publishing country United States
    Document type Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 6165-7
    ISSN 1432-1750 ; 0341-2040
    ISSN (online) 1432-1750
    ISSN 0341-2040
    DOI 10.1007/s00408-004-2506-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Lipoprotein(a) in Youth and Prediction of Major Cardiovascular Outcomes in Adulthood.

    Raitakari, Olli / Kartiosuo, Noora / Pahkala, Katja / Hutri-Kähönen, Nina / Bazzano, Lydia A / Chen, Wei / Urbina, Elaine M / Jacobs, David R / Sinaiko, Alan / Steinberger, Julia / Burns, Trudy / Daniels, Stephen R / Venn, Alison / Woo, Jessica G / Dwyer, Terry / Juonala, Markus / Viikari, Jorma

    Circulation

    2022  Volume 147, Issue 1, Page(s) 23–31

    Abstract: Background: Elevated lipoprotein(a) [Lp(a)] is a common risk factor for cardiovascular disease outcomes with unknown mechanisms. We examined its potential role in identifying youths who are at increased risk of developing adult atherosclerotic ... ...

    Abstract Background: Elevated lipoprotein(a) [Lp(a)] is a common risk factor for cardiovascular disease outcomes with unknown mechanisms. We examined its potential role in identifying youths who are at increased risk of developing adult atherosclerotic cardiovascular disease (ASCVD).
    Methods: Lp(a) levels measured in youth 9 to 24 years of age were linked to adult ASCVD and carotid intima-media thickness in the YFS (Cardiovascular Risk in Young Finns Study), in which 95 of the original 3596 participants (2.7%) recruited as children have been diagnosed with ASCVD at a median of 47 years of age. Results observed in YFS were replicated with the use of data for White participants from the BHS (Bogalusa Heart Study). In BHS, 587 White individuals had data on youth Lp(a) (measured at 8-17 years of age) and information on adult events, including 15 cases and 572 noncases. Analyses were performed with the use of Cox proportional hazard regression.
    Results: In YFS, those who had been exposed to high Lp(a) level in youth [defined as Lp(a) ≥30 mg/dL] had ≈2 times greater risk of developing adult ASCVD compared with nonexposed individuals (hazard ratio, 2.0 [95% CI, 1.4-2.6]). Youth risk factors, including Lp(a), low-density lipoprotein cholesterol, body mass index, and smoking, were all independently associated with higher risk. In BHS, in an age- and sex-adjusted model, White individuals who had been exposed to high Lp(a) had 2.5 times greater risk (95% CI, 0.9-6.8) of developing adult ASCVD compared with nonexposed individuals. When also adjusted for low-density lipoprotein cholesterol and body mass index, the risk associated with high Lp(a) remained unchanged (hazard ratio, 2.4 [95% CI, 0.8-7.3]). In a multivariable model for pooled data, individuals exposed to high Lp(a) had 2.0 times greater risk (95% CI, 1.0-3.7) of developing adult ASCVD compared with nonexposed individuals. No association was detected between youth Lp(a) and adult carotid artery thickness in either cohort or pooled data.
    Conclusions: Elevated Lp(a) level identified in youth is a risk factor for adult atherosclerotic cardiovascular outcomes but not for increased carotid intima-media thickness.
    MeSH term(s) Adult ; Child ; Humans ; Adolescent ; Lipoprotein(a) ; Carotid Intima-Media Thickness ; Cardiovascular Diseases/diagnosis ; Cardiovascular Diseases/epidemiology ; Risk Assessment ; Risk Factors ; Atherosclerosis/epidemiology ; Atherosclerosis/diagnosis ; Cholesterol, LDL
    Chemical Substances Lipoprotein(a) ; Cholesterol, LDL
    Language English
    Publishing date 2022-11-28
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80099-5
    ISSN 1524-4539 ; 0009-7322 ; 0069-4193 ; 0065-8499
    ISSN (online) 1524-4539
    ISSN 0009-7322 ; 0069-4193 ; 0065-8499
    DOI 10.1161/CIRCULATIONAHA.122.060667
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Cigarette smoke-induced airway inflammation as sampled by the expired breath condensate.

    Dwyer, Terry M

    The American journal of the medical sciences

    2003  Volume 326, Issue 4, Page(s) 174–178

    Abstract: Airways exposed to smoke respond with inflammatory processes. The airway inflammation generally present in smokers causes persistent cough and phlegm production, reactive airway disease, and tissue infiltration by inflammatory cells. Although the short- ... ...

    Abstract Airways exposed to smoke respond with inflammatory processes. The airway inflammation generally present in smokers causes persistent cough and phlegm production, reactive airway disease, and tissue infiltration by inflammatory cells. Although the short-term response may be protective, long-term pathological consequences include swelling of the airway epithelium, mucus hypersecretion, and increased airway reactivity characteristic of chronic bronchitis and chronic obstructive lung disease and the tissue destruction characteristic of emphysema. The natural history of these diseases is poorly understood, because human airway tissue is available for study only at autopsy, from surgical specimens, or from procedures such as bronchoscopy or thoracotomy. A noninvasive method of monitoring the inflammation is by analyzing expired breath condensate, which contains a diluted sample of airway surface liquid. The study of expired breath condensate may offer a more practical approach to sampling airway chemistry and make it possible to study the detailed inflammatory response to airborne particulates.
    MeSH term(s) Breath Tests ; Emphysema ; Genetic Predisposition to Disease ; Humans ; Inflammation/diagnosis ; Inflammation/etiology ; Inflammation/pathology ; Reactive Oxygen Species/analysis ; Respiratory Tract Diseases/diagnosis ; Respiratory Tract Diseases/etiology ; Respiratory Tract Diseases/pathology ; Smoke/adverse effects ; Smoke/analysis ; Nicotiana ; alpha 1-Antitrypsin
    Chemical Substances Reactive Oxygen Species ; Smoke ; alpha 1-Antitrypsin
    Language English
    Publishing date 2003-10-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 82078-7
    ISSN 1538-2990 ; 0002-9629
    ISSN (online) 1538-2990
    ISSN 0002-9629
    DOI 10.1097/00000441-200310000-00004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Relative and Absolute Expression Analysis of MicroRNAs Associated with Luminal A Breast Cancer- A Comparison.

    Arabkari, Vahid / Clancy, Eoin / Dwyer, Róisín M / Kerin, Michael J / Kalinina, Olga / Holian, Emma / Newell, John / Smith, Terry J

    Pathology oncology research : POR

    2019  Volume 26, Issue 2, Page(s) 833–844

    Abstract: MicroRNAs, as small non-coding regulatory RNAs, play crucial roles in various aspects of breast cancer biology. They have prognostic and diagnostic value, which makes them very interesting molecules to investigate. Reverse transcriptase quantitative ... ...

    Abstract MicroRNAs, as small non-coding regulatory RNAs, play crucial roles in various aspects of breast cancer biology. They have prognostic and diagnostic value, which makes them very interesting molecules to investigate. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is the gold standard method to analyse miRNA expression in breast cancer patients. This study investigated two RT-qPCR methods (absolute and relative) to determine the expression of ten miRNAs in whole blood samples obtained from luminal A breast cancer patients compared to healthy controls. Whole blood samples were collected from 38 luminal A breast cancer patients and 20 healthy controls in Paxgene blood RNA tubes. Total RNA was extracted and analysed by relative and absolute RT-qPCR. For relative RT-qPCR, miR-16 was used as an endogenous control. For absolute RT-qPCR, standard curves were generated using synthetic miRNA oligonucleotides to determine the absolute copy number of each miRNA. Of the ten miRNAs that were analysed, the absolute RT-qPCR method identified six miRNAs (miR-16, miR-145, miR-155, miR-451a, miR-21 and miR-486) that were upregulated and one miRNA (miR-195) that was downregulated. ROC curve and AUC analysis of the data found that the combination of three miRNAs (miR-145, miR-195 and miR-486) had the best diagnostic value for luminal A breast cancer with an AUC of 0.875, with 76% sensitivity and 81% specificity. On the other hand, the relative RT-qPCR method identified two miRNAs (miR-155 and miR-486) that were upregulated and miR-195, which was downregulated. Using this approach, the combination of three miRNAs (miR-155, miR-195 and miR-486) was showed to have an AUC of 0.657 with 65% sensitivity and 69% specificity. We conclude that miR-16 is not a suitable normalizer for the relative expression profiling of miRNAs in luminal A breast cancer patients. Compared to relative quantification, absolute quantification assay is a better method to determine the expression level of circulating miRNAs in Luminal A breast cancer.
    MeSH term(s) Adult ; Biomarkers, Tumor/genetics ; Breast Neoplasms/diagnosis ; Breast Neoplasms/genetics ; Female ; Humans ; MicroRNAs/analysis ; MicroRNAs/biosynthesis ; Middle Aged ; Sensitivity and Specificity
    Chemical Substances Biomarkers, Tumor ; MicroRNAs
    Language English
    Publishing date 2019-03-06
    Publishing country Switzerland
    Document type Comparative Study ; Journal Article
    ZDB-ID 1375979-6
    ISSN 1532-2807 ; 1219-4956
    ISSN (online) 1532-2807
    ISSN 1219-4956
    DOI 10.1007/s12253-019-00627-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Amplification-free detection of microRNAs via a rapid microarray-based sandwich assay.

    Clancy, Eoin / Burke, Martina / Arabkari, Vahid / Barry, Thomas / Kelly, Helena / Dwyer, Róisín M / Kerin, Michael J / Smith, Terry J

    Analytical and bioanalytical chemistry

    2017  Volume 409, Issue 14, Page(s) 3497–3505

    Abstract: The detection and profiling of microRNAs are of great interest in disease diagnosis and prognosis. In this paper, we present a method for the rapid amplification-free detection of microRNAs from total RNA samples. In a two-step sandwich assay approach, ... ...

    Abstract The detection and profiling of microRNAs are of great interest in disease diagnosis and prognosis. In this paper, we present a method for the rapid amplification-free detection of microRNAs from total RNA samples. In a two-step sandwich assay approach, fluorescently labeled reporter probes were first hybridized with their corresponding target microRNAs. The reaction mix was then added to a microarray to enable their specific capture and detection. Reporter probes were T
    Language English
    Publishing date 2017-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 201093-8
    ISSN 1618-2650 ; 0016-1152 ; 0372-7920
    ISSN (online) 1618-2650
    ISSN 0016-1152 ; 0372-7920
    DOI 10.1007/s00216-017-0298-6
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