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  1. Article ; Online: Confirmation of increased and more severe adolescent mental health-related in-patient admissions in the COVID-19 pandemic aftermath: A 2-year follow-up study.

    Marin, Dario / Di Gennaro, Gianfranco / Baracetti, Margherita / Zanetti, Rossella / Balestrieri, Matteo / Cogo, Paola / Colizzi, Marco

    Psychiatry research communications

    2023  Volume 3, Issue 2, Page(s) 100119

    Abstract: COVID-19 pandemic may have affected youth's mental wellbeing. Youth admissions for mental health emergencies over the 2-year period following the COVID-19 outbreak (March 2020-February 2022) were compared to those occurring in the same period of 2018- ... ...

    Abstract COVID-19 pandemic may have affected youth's mental wellbeing. Youth admissions for mental health emergencies over the 2-year period following the COVID-19 outbreak (March 2020-February 2022) were compared to those occurring in the same period of 2018-2020, with reference to individual and clinical data. The study identified 30 admissions in the pre-pandemic period and 65 (+116.7%) in the post-pandemic period, with the latter being younger, less likely to have a personal psychiatric history, and more likely to receive psychopharmacological treatment. A higher likelihood of earlier,
    Language English
    Publishing date 2023-03-13
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2772-5987
    ISSN (online) 2772-5987
    DOI 10.1016/j.psycom.2023.100119
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  2. Article ; Online: Mammographic breast density and survival in women with invasive breast cancer.

    Pizzato, Margherita / Carioli, Greta / Rosso, Stefano / Zanetti, Roberto / La Vecchia, Carlo

    Cancer causes & control : CCC

    2022  Volume 33, Issue 9, Page(s) 1207–1213

    Abstract: Purpose: We explored the under-debate association between mammographic breast density (MBD) and survival.: Methods: From the Piedmont Cancer Registry, we identified 693 invasive breast cancer (BC) cases. We analyzed the overall survival in strata of ... ...

    Abstract Purpose: We explored the under-debate association between mammographic breast density (MBD) and survival.
    Methods: From the Piedmont Cancer Registry, we identified 693 invasive breast cancer (BC) cases. We analyzed the overall survival in strata of MBD through the Kaplan-Meier method. Using the Cox proportional hazards model, we estimated the hazard ratios (HRs) of death; using the cause-specific hazards regression model, we estimated the HRs of BC-related and other causes of death. Models included term for Breast Imaging-Reporting and Data System (BI-RADS) MBD (categorized as BI-RADS 1 and BI-RADS 2-4) and were adjusted for selected patient and tumour characteristics.
    Results: There were 102 deaths, of which 49 were from BC. After 5 years, the overall survival was 69% in BI-RADS 1 and 88% in BI-RADS 2-4 (p < 0.01). Compared to BI-RADS 2-4, the HRs of death for BI-RADS 1 were 1.65 (95% CI 1.06-2.58) in the crude model and 1.35 (95% CI 0.84-2.16) in the fully adjusted model. Compared to BI-RADS 2-4, the fully adjusted HRs for BI-RADS 1 were 1.52 (95% CI 0.74-3.13) for BC-related death and 1.83 (95% CI 0.84-4.00) for the other causes of death.
    Conclusion: Higher MBD is one of the strongest independent risk factors for BC, but it seems not to have an unfavorable impact on survival.
    MeSH term(s) Breast Density ; Breast Neoplasms/diagnostic imaging ; Breast Neoplasms/pathology ; Female ; Humans ; Mammography/methods ; Risk Factors
    Language English
    Publishing date 2022-06-13
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1064022-8
    ISSN 1573-7225 ; 0957-5243
    ISSN (online) 1573-7225
    ISSN 0957-5243
    DOI 10.1007/s10552-022-01590-x
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    Zs.A 3375: Show issues Location:
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  3. Article ; Online: Cigarettes smoking and androgen receptor-positive breast cancer.

    Pizzato, Margherita / Carioli, Greta / Rosso, Stefano / Zanetti, Roberto / Negri, Eva / La Vecchia, Carlo

    European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)

    2020  Volume 30, Issue 6, Page(s) 469–471

    Abstract: Objectives: Cigarette smoking is related to higher levels of circulating androgens, but its association with androgen receptor (AR) status is still unaddressed.: Methods: We analysed, with a case-only approach, smoking habits according to AR status ... ...

    Abstract Objectives: Cigarette smoking is related to higher levels of circulating androgens, but its association with androgen receptor (AR) status is still unaddressed.
    Methods: We analysed, with a case-only approach, smoking habits according to AR status in 112 cases of invasive female breast cancer, from the Piedmont Cancer Registry. We used a multivariate logistic regression model to estimate the odds ratio (OR) and the corresponding confidence interval (CI).
    Results: The OR of AR-positive breast cancer (versus AR-negative) for ever smokers (versus never) was 2.85 (95% CI 1.02-7.96).
    Conclusion: Smoking is related to AR-positive breast cancer.
    MeSH term(s) Breast Neoplasms/epidemiology ; Breast Neoplasms/etiology ; Female ; Humans ; Odds Ratio ; Receptors, Androgen ; Smoking/adverse effects ; Smoking/epidemiology ; Tobacco Products
    Chemical Substances Receptors, Androgen
    Language English
    Publishing date 2020-12-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 1137033-6
    ISSN 1473-5709 ; 0959-8278
    ISSN (online) 1473-5709
    ISSN 0959-8278
    DOI 10.1097/CEJ.0000000000000661
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    Zs.A 3621: Show issues Location:
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  4. Article ; Online: Therapeutic Strategies and Outcomes in Neuropsychiatric Systemic Lupus Erythematosus: An International Multicenter Retrospective Study.

    Bortoluzzi, Alessandra / Fanouriakis, Antonis / Silvagni, Ettore / Appenzeller, Simone / Carli, Linda / Carrara, Greta / Cauli, Alberto / Conti, Fabrizio / Costallat, Lilian Teresa Lavras / De Marchi, Ginevra / Doria, Andrea / Fredi, Micaela / Franceschini, Franco / Garaffoni, Carlo / Hanly, John G / Mosca, Marta / Murphy, Elana / Piga, Matteo / Quartuccio, Luca /
    Scirè, Carlo Alberto / Tomietto, Paola / Truglia, Simona / Zanetti, Anna / Zen, Margherita / Bertsias, George / Govoni, Marcello

    Rheumatology (Oxford, England)

    2024  

    Abstract: Objectives: The management of neuropsychiatric systemic lupus erythematosus (NPSLE) poses considerable challenges due to limited clinical trials. Therapeutic decisions are customized based on suspected pathogenic mechanisms and symptom severity. This ... ...

    Abstract Objectives: The management of neuropsychiatric systemic lupus erythematosus (NPSLE) poses considerable challenges due to limited clinical trials. Therapeutic decisions are customized based on suspected pathogenic mechanisms and symptom severity. This study aimed to investigate therapeutic strategies and disease outcome for patients with NPSLE experiencing their first neuropsychiatric (NP) manifestation.
    Methods: This retrospective cohort study defined NP events according to the American College of Rheumatology case definition, categorizing them into three clusters: central/diffuse, central/focal and peripheral. Clinical judgment and a validated attribution algorithm were used for NP event attribution. Data included demographic variables, SLE disease activity index, cumulative organ damage, and NP manifestation treatments. The clinical outcome of all NP events was determined by a physician seven-point Likert scale. Predictors of clinical improvement/resolution were investigated in a multivariable logistic regression analysis.
    Results: The analysis included 350 events. Immunosuppressants and corticosteroids were more frequently initiated/escalated for SLE-attributed central diffuse or focal NP manifestations. At 12 months of follow-up, 64% of patients showed a clinical improvement in NP manifestations. Focal central events and SLE-attributed manifestations correlated with higher rates of clinical improvement. Patients with NP manifestations attributed to SLE according to clinical judgment and treated with immunosuppressants had a significantly higher probability of achieving clinical response (OR 2.55, 95%CI 1.06-6.41, p= 0.04). Age at diagnosis and focal central events emerged as additional response predictors.
    Conclusion: NP manifestations attributed to SLE by clinical judgment and treated with immunosuppressants demonstrated improved 12-month outcomes. This underscores the importance of accurate attribution and timely diagnosis of NPSLE.
    Language English
    Publishing date 2024-02-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 1464822-2
    ISSN 1462-0332 ; 1462-0324
    ISSN (online) 1462-0332
    ISSN 1462-0324
    DOI 10.1093/rheumatology/keae119
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    Zs.B 240: Show issues Location:
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    Zs.MO 497: Show issues

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  5. Article ; Online: The impact of selected risk factors among breast cancer molecular subtypes: a case-only study.

    Pizzato, Margherita / Carioli, Greta / Rosso, Stefano / Zanetti, Roberto / La Vecchia, Carlo

    Breast cancer research and treatment

    2020  Volume 184, Issue 1, Page(s) 213–220

    Abstract: Purpose: Breast cancer (BC) risk factors have been differentially associated with BC subtypes, but quantification is still undefined. Therefore, we compared selected risk factors with BC subtypes, using a case-case approach.: Methods: We retrieved ... ...

    Abstract Purpose: Breast cancer (BC) risk factors have been differentially associated with BC subtypes, but quantification is still undefined. Therefore, we compared selected risk factors with BC subtypes, using a case-case approach.
    Methods: We retrieved 1321 invasive female BCs from the Piedmont Cancer Registry. Through record linkage of clinical records, we obtained data on estrogen (Er) and progesterone (Pr) receptors, Ki67 and HER2+ status, BC family history, breast imaging reporting and data system (BI-RADS) density, reproductive risk factors and education. We defined BC subtypes as follows : luminal A (Er+ and/or Pr+ , HER2- , low Ki67), luminal BH- (Er+ and/or Pr + , HER2- , Ki67 high), luminal BH+ (Er+ and/or Pr + , HER2+), HER2+ (Er - , Pr - , HER2+), ) and triple negative (Er - , Pr - , HER2-). Using a multinomial regression model, we estimated the odds ratios (ORs) for selected BC risk factors considering luminal A as reference.
    Results: For triple negative, the OR for BC family history was 1.83 (95% confidence interval (CI) 1.13-2.97). Compared to BI-RADS 1, for triple negative, the OR for BI-RADS 2 was 0.56 (95% CI 0.27-1.14) and for BI-RADS 3-4 was 0.37 (95% CI 0.15-0.88); for luminal BH +, the OR for BI-RADS 2 was 2.36 (95% CI 1.08-5.11). For triple negative, the OR for high education was 1.78 (95% CI 1.03-3.07), and for late menarche, the OR was 1.69 (95% CI 1.02-2.81). For luminal BH + , the OR for parous women was 0.56 (95% CI 0.34-0.92).
    Conclusions: This study supported BC etiologic heterogeneity across subtypes, particularly for triple negative.
    MeSH term(s) Biomarkers, Tumor ; Breast Neoplasms/epidemiology ; Breast Neoplasms/genetics ; Female ; Humans ; Odds Ratio ; Receptor, ErbB-2/genetics ; Receptors, Progesterone/genetics ; Reproductive History ; Risk Factors
    Chemical Substances Biomarkers, Tumor ; Receptors, Progesterone ; Receptor, ErbB-2 (EC 2.7.10.1)
    Language English
    Publishing date 2020-08-26
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604563-7
    ISSN 1573-7217 ; 0167-6806
    ISSN (online) 1573-7217
    ISSN 0167-6806
    DOI 10.1007/s10549-020-05820-1
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    Zs.A 1655: Show issues Location:
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  6. Article: The role of cathelicidins in the innate host defenses of mammals.

    Zanetti, Margherita

    Current issues in molecular biology

    2005  Volume 7, Issue 2, Page(s) 179–196

    Abstract: The cathelicidin peptides comprise one of several families of antimicrobial peptides that are found in neutrophils and epithelia as components of the early host defenses of mammals against infection. All cathelicidin family members are synthesized and ... ...

    Abstract The cathelicidin peptides comprise one of several families of antimicrobial peptides that are found in neutrophils and epithelia as components of the early host defenses of mammals against infection. All cathelicidin family members are synthesized and stored in cells as two-domain proteins. These are split on demand to produce a cathelin protein and an antimicrobial peptide. Accumulating evidence indicates that both the cathelin portion and the C-terminal peptide exert biological activities connected with host protection. This review presents an overview of the structure and biology of cathelicidins and discusses recent progress in cathelicidin research with emphasis on the functional properties and role in host defense of the human cathelicidin hCAP18/LL-37. Although investigators initially concentrated their attention on antibiotic activity, it is becoming clear now that LL-37 is a multifunctional molecule that may mediate various host responses, and thus represents an essential component of the innate immune system in humans.
    MeSH term(s) Animals ; Antimicrobial Cationic Peptides/chemistry ; Antimicrobial Cationic Peptides/genetics ; Antimicrobial Cationic Peptides/immunology ; Antimicrobial Cationic Peptides/metabolism ; Gene Expression Regulation ; Humans ; Immunity, Innate ; Mammals/immunology
    Chemical Substances Antimicrobial Cationic Peptides ; ropocamptide (3DD771JO2H)
    Language English
    Publishing date 2005-07-20
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2000024-8
    ISSN 1467-3037
    ISSN 1467-3037
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    Zs.A 5122: Show issues Location:
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  7. Article: Cathelicidins, multifunctional peptides of the innate immunity.

    Zanetti, Margherita

    Journal of leukocyte biology

    2004  Volume 75, Issue 1, Page(s) 39–48

    Abstract: Cathelicidins comprise a family of mammalian proteins containing a C-terminal cationic antimicrobial domain that becomes active after being freed from the N-terminal cathelin portion of the holoprotein. Many other members of this family have been ... ...

    Abstract Cathelicidins comprise a family of mammalian proteins containing a C-terminal cationic antimicrobial domain that becomes active after being freed from the N-terminal cathelin portion of the holoprotein. Many other members of this family have been identified since the first cathelicidin sequences were reported 10 years ago. The mature peptides generally show a wide spectrum of antimicrobial activity and, more recently, some of them have also been found to exert other biological activities. The human cathelicidin peptide LL-37 is chemotactic for neutrophils, monocytes, mast cells, and T cells; induces degranulation of mast cells; alters transcriptional responses in macrophages; stimulates wound vascularization and re-epithelialization of healing skin. The porcine PR-39 has also been involved in a variety of processes, including promotion of wound repair, induction of angiogenesis, neutrophils chemotaxis, and inhibition of the phagocyte NADPH oxidase activity, whereas the bovine BMAP-28 induces apoptosis in transformed cell lines and activated lymphocytes and may thus help with clearance of unwanted cells at inflammation sites. These multiple actions provide evidence for active participation of cathelicidin peptides in the regulation of the antimicrobial host defenses.
    MeSH term(s) Animals ; Antimicrobial Cationic Peptides/physiology ; Blood Proteins/physiology ; Cattle ; Chemotaxis, Leukocyte/physiology ; Humans ; Immunity, Innate/physiology ; Macrophages/physiology ; Protein Precursors/physiology ; Proteins/physiology ; T-Lymphocytes/immunology
    Chemical Substances Antimicrobial Cationic Peptides ; BMAP-28 ; Blood Proteins ; Protein Precursors ; Proteins ; cathelicidin 2 protein, mammal ; cathelicidin 3 protein, Equus caballus
    Language English
    Publishing date 2004-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 605722-6
    ISSN 1938-3673 ; 0741-5400
    ISSN (online) 1938-3673
    ISSN 0741-5400
    DOI 10.1189/jlb.0403147
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    Zs.A 603: Show issues Location:
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  8. Article ; Online: DNMT3A epigenetically regulates key microRNAs involved in epithelial-to-mesenchymal transition in prostate cancer.

    Mancini, Monica / Grasso, Margherita / Muccillo, Livio / Babbio, Federica / Precazzini, Francesca / Castiglioni, Ilaria / Zanetti, Valentina / Rizzo, Francesca / Pistore, Christian / De Marino, Maria Giovanna / Zocchi, Michele / Del Vescovo, Valerio / Licursi, Valerio / Giurato, Giorgio / Weisz, Alessandro / Chiarugi, Paola / Sabatino, Lina / Denti, Michela Alessandra / Bonapace, Ian Marc

    Carcinogenesis

    2021  Volume 42, Issue 12, Page(s) 1449–1460

    Abstract: Epithelial-to-mesenchymal transition (EMT) is involved in prostate cancer (PCa) metastatic progression, and its plasticity suggests epigenetic implications. Deregulation of DNA methyltransferases (DNMTs) and several microRNAs (miRNAs) plays a relevant ... ...

    Abstract Epithelial-to-mesenchymal transition (EMT) is involved in prostate cancer (PCa) metastatic progression, and its plasticity suggests epigenetic implications. Deregulation of DNA methyltransferases (DNMTs) and several microRNAs (miRNAs) plays a relevant role in EMT, but their interplay has not been clarified yet. In this study, we provide evidence that DNMT3A interaction with several miRNAs has a central role in an ex vivo EMT PCa model obtained via exposure of PC3 cells to conditioned media from cancer-associated fibroblasts. The analysis of the alterations of the miRNA profile shows that miR-200 family (miR-200a/200b/429, miR-200c/141), miR-205 and miR-203, known to modulate key EMT factors, are down-regulated and hyper-methylated at their promoters. DNMT3A (mainly isoform a) is recruited onto these miRNA promoters, coupled with the increase of H3K27me3/H3K9me3 and/or the decrease of H3K4me3/H3K36me3. Most interestingly, our results reveal the differential expression of two DNMT3A isoforms (a and b) during ex vivo EMT and a regulatory feedback loop between miR-429 and DNMT3A that can promote and sustain the transition towards a more mesenchymal phenotype. We demonstrate the ability of miR-429 to target DNMT3A 3'UTR and modulate the expression of EMT factors, in particular ZEB1. Survey of the PRAD-TCGA dataset shows that patients expressing an EMT-like signature are indeed characterized by down-regulation of the same miRNAs with a diffused hyper-methylation at miR-200c/141 and miR-200a/200b/429 promoters. Finally, we show that miR-1260a also targets DNMT3A, although it does not seem to be involved in EMT in PCa.
    MeSH term(s) Binding Sites ; Chromatin Immunoprecipitation ; Computational Biology/methods ; DNA Methylation ; DNA Methyltransferase 3A/metabolism ; Disease Susceptibility ; Epigenesis, Genetic ; Epithelial-Mesenchymal Transition/genetics ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; MicroRNAs/genetics ; Promoter Regions, Genetic ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/metabolism ; Prostatic Neoplasms/pathology ; Protein Binding ; RNA Interference ; Tumor Microenvironment/drug effects ; Tumor Microenvironment/genetics
    Chemical Substances DNMT3A protein, human ; MicroRNAs ; DNA Methyltransferase 3A (EC 2.1.1.37)
    Language English
    Publishing date 2021-10-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 603134-1
    ISSN 1460-2180 ; 0143-3334
    ISSN (online) 1460-2180
    ISSN 0143-3334
    DOI 10.1093/carcin/bgab101
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    Zs.A 1558: Show issues Location:
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  9. Article ; Online: Modulation of cytokine gene expression by cathelicidin BMAP-28 in LPS-stimulated and -unstimulated macrophages.

    D'Este, Francesca / Tomasinsig, Linda / Skerlavaj, Barbara / Zanetti, Margherita

    Immunobiology

    2012  Volume 217, Issue 10, Page(s) 962–971

    Abstract: Apart from direct bacterial killing, antimicrobial host defence peptides (HDPs) exert various other biological activities that also include modulation of immune responses to infection. The bovine cathelicidin BMAP-28 has been extensively studied with ... ...

    Abstract Apart from direct bacterial killing, antimicrobial host defence peptides (HDPs) exert various other biological activities that also include modulation of immune responses to infection. The bovine cathelicidin BMAP-28 has been extensively studied with regard to its direct antibacterial activity while little is known about its effects on immune cell function. We have investigated its ability to affect inflammatory pathways and to influence the proinflammatory response induced by LPS in RAW 264.7 macrophages, in terms of modulation of TLR4 activation and cytokine gene induction. BMAP-28 on its own elicited ERK1/2, p38 and NF-κB activation leading to upregulation of IL-1β gene expression in these cells, suggesting it has the capacity to activate selected cellular pathways through direct effects on macrophages. As expected based on its in vitro LPS-binding properties, BMAP-28 blocked LPS-induced cytokine gene expression when added to the cell culture in combination with LPS. However it enhanced the induction of IL-1β and IL-6 genes and suppressed that of IFN-β when added prior to or following LPS stimulation over a 30-60 min time interval, or when co-administered with taxol as another TLR4 stimulant. It did not inhibit the expression of IFN-β induced by the TLR3 ligand poly(I:C). Overall these results, and the fact that BMAP-28 increased the LPS-stimulated activation of NF-κB while diminishing that of IRF-3, suggest that the peptide potentiates the early TLR4-mediated proinflammatory cytokine response while inhibiting the TLR4/TRAM/TRIF signaling pathway leading to IRF-3 activation and IFN-β gene expression. Using a TLR4-specific antibody we also found that BMAP-28 decreased the LPS-induced internalization of surface TLR4 required for initiating the TRAM/TRIF signaling pathway, which provides a mechanism for the inhibitory effect of the peptide on the TLR4/TRAM/TRIF pathway.
    MeSH term(s) Animals ; Cell Line ; Cytokines/genetics ; Cytokines/immunology ; Gene Expression Regulation/drug effects ; Inflammation/genetics ; Inflammation/immunology ; Interferon Regulatory Factor-3/metabolism ; Lipopolysaccharides/immunology ; Lipopolysaccharides/metabolism ; Macrophages/drug effects ; Macrophages/immunology ; Macrophages/metabolism ; Mice ; NF-kappa B/metabolism ; Protein Binding/immunology ; Proteins/metabolism ; Proteins/pharmacology ; Signal Transduction ; Toll-Like Receptor 3/metabolism ; Toll-Like Receptor 4/metabolism
    Chemical Substances BMAP-28 ; Cytokines ; Interferon Regulatory Factor-3 ; Lipopolysaccharides ; NF-kappa B ; Proteins ; Toll-Like Receptor 3 ; Toll-Like Receptor 4
    Language English
    Publishing date 2012-10
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 563292-4
    ISSN 1878-3279 ; 0171-2985
    ISSN (online) 1878-3279
    ISSN 0171-2985
    DOI 10.1016/j.imbio.2012.01.010
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  10. Article: The Integration of qSOFA with Clinical Variables and Serum Biomarkers Improves the Prognostic Value of qSOFA Alone in Patients with Suspected or Confirmed Sepsis at ED Admission.

    Mearelli, Filippo / Barbati, Giulia / Casarsa, Chiara / Giansante, Carlo / Breglia, Andrea / Spica, Andrea / Moras, Cristina / Olivieri, Gaia / Occhipinti, Alessandro Agostino / De Nardo, Margherita / Spagnol, Francesca / Fiotti, Nicola / Di Girolamo, Filippo Giorgio / Ruscio, Maurizio / Castello, Luigi Mario / Colonetti, Efrem / Marino, Rossella / Ronco, Claudio / Zanetti, Michela /
    Lupia, Enrico / Muiesan, Maria Lorenza / Di Somma, Salvatore / Avanzi, Gian Carlo / Biolo, Gianni

    Journal of clinical medicine

    2020  Volume 9, Issue 4

    Abstract: Background: The prognostic value of quick sepsis-related organ failure assessment (qSOFA) outside intensive care units has been criticized. Therefore, we aimed to improve its ability in predicting 30-day all-cause mortality, and in ruling out the cases ... ...

    Abstract Background: The prognostic value of quick sepsis-related organ failure assessment (qSOFA) outside intensive care units has been criticized. Therefore, we aimed to improve its ability in predicting 30-day all-cause mortality, and in ruling out the cases at high risk of death among patients with suspected or confirmed sepsis at emergency department (ED) admission.
    Methods: This study is a secondary analysis of a prospective multicenter study. We built three predictive models combining qSOFA with the clinical variables and serum biomarkers that resulted in an independent association with 30-day mortality, in both 848 undifferentiated patients (Group 1) and in 545 patients definitively diagnosed with sepsis (Group 2). The models reaching the highest negative predictive value (NPV) with the minimum expenditure of biomarkers in Group 1 and in Group 2 were validated in two cohorts of patients initially held out due to missing data.
    Results: In terms of the area under the receiver-operating characteristic curve, all six models significantly exceeded qSOFA in predicting prognosis. An "extended" qSOFA (eqSOFA1) in Group 1 and an eqSOFA2 integrated with C-reactive protein and mid-regional proadrenomedullin (eqSOFA2+CRP+MR-proADM) in Group 2 reached the best NPV (0.94 and 0.93, respectively) and ease of use. eqSOFA1 and eqSOFA2+CRP+MR-proADM performed equally well in both the inception and validation cohorts.
    Conclusions: We have derived and validated two prognostic models that outweigh qSOFA in predicting mortality and in identifying the low risk of death among patients with suspected or confirmed sepsis at ED admission.
    Language English
    Publishing date 2020-04-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm9041205
    Database MEDical Literature Analysis and Retrieval System OnLINE

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