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Article ; Online: Evaluation and Treatment of the Child with Acute Back Pain.

Murphy, Kevin P / Sanders, Cristina / Rabatin, Amy E

2023 Volume 70, Issue 3, Page(s) 545–574

Abstract: Back pain is common, in up to 30% of children, increasing with age. Eighty percent is benign, mechanical type, improving within 2 weeks of conservative care. Required for those not improving is in-depth evaluation, including MRI, laboratory, and peer ... ...

Abstract	Back pain is common, in up to 30% of children, increasing with age. Eighty percent is benign, mechanical type, improving within 2 weeks of conservative care. Required for those not improving is in-depth evaluation, including MRI, laboratory, and peer consultations. Spondylolysis and spondylolisthesis comprise almost 10% of pediatric back pain, often caused by lumbar hyperextension activities and treated conservatively in most cases. Osteoid osteomas and osteoblastomas constitute the most common benign spinal tumors in childhood. Aggressive and malignant tumors of the spine are rare but when present require tertiary care referral and a comprehensive oncology team for optimal life-sustaining outcomes.
MeSH term(s)	Humans ; Child ; Back Pain/etiology ; Spondylolysis ; Spondylolisthesis/complications ; Magnetic Resonance Imaging/adverse effects ; Lumbosacral Region
Language	English
Publishing date	2023-03-21
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	215711-1
ISSN	1557-8240 ; 0031-3955
ISSN (online)	1557-8240
ISSN	0031-3955
DOI	10.1016/j.pcl.2023.01.013
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Article ; Online: Risk Factors for Stiffness After Surgical Treatment of Pediatric Humeral Lateral Condyle Fractures: Results of a Multicenter Study.

Tippabhatla, Abhishek / Torres-Izquierdo, Beltran / Pereira, Daniel E / Goldstein, Rachel / Sanders, Julia / Neal, Kevin / Bellaire, Laura / Denning, Jaime / Hosseinzadeh, Pooya

Journal of pediatric orthopedics

2024 Volume 44, Issue 4, Page(s) 208–212

Abstract: Introduction: Pediatric humeral lateral condyle fractures are the second most common elbow fractures. Their treatment presents challenges due to physeal and intra-articular involvement. Postoperative stiffness is a common concern that can limit limb ... ...

Abstract	Introduction: Pediatric humeral lateral condyle fractures are the second most common elbow fractures. Their treatment presents challenges due to physeal and intra-articular involvement. Postoperative stiffness is a common concern that can limit limb functionality. This study aims to identify risk factors for postoperative stiffness in a large cohort of these fractures across multiple institutions. Methods: A large, multicenter retrospective review of medical records from 6 level I trauma centers was conducted. Data from children aged 1 to 12 years with lateral condyle fractures treated between 2005 and 2019 were collected. Elbow stiffness was defined in the present study as having a limited elbow ROM that led to requiring a physical or occupational therapy referral or needing surgical treatment to address stiffness. Relevant patient demographics, fracture characteristics, treatment approaches, and complications were analyzed. Results: Six hundred sixty-five fractures were analyzed. The average patient age was 8.8 years with 21% experiencing stiffness. The stiffness group had older patients, a higher incidence of elbow dislocations, a higher rate of open reduction, and more severe fracture patterns. Multivariate regression analysis identified open reduction, increased age, and concurrent elbow dislocation as significant risk factors for stiffness. Patients with stiffness commonly utilized only physical or occupational therapy (96%), while a small percentage (4%) required surgical interventions. Conclusions: This study highlights the risk factors for postoperative stiffness in pediatric humeral lateral condyle fractures, namely increased age, concomitant elbow dislocation, and treatment with open reduction. Families of older patients or severe fracture patterns requiring open reduction and those with concurrent elbow instability should be counseled about their increased risk of stiffness. The authors recommend initially attempting a closed reduction in high-risk patients to help mitigate the risk of postoperative stiffness. Early initiation of range of motion exercises may also be beneficial for at-risk patients. Level of evidence: Level III: Therapeutic studies-Investigating the results of treatment.
MeSH term(s)	Child ; Humans ; Elbow Joint/surgery ; Joint Instability/etiology ; Humeral Fractures/complications ; Humerus ; Joint Dislocations/surgery ; Retrospective Studies ; Fracture Fixation, Internal/adverse effects ; Fracture Fixation, Internal/methods ; Range of Motion, Articular ; Treatment Outcome
Language	English
Publishing date	2024-01-29
Publishing country	United States
Document type	Multicenter Study ; Journal Article
ZDB-ID	604642-3
ISSN	1539-2570 ; 0271-6798
ISSN (online)	1539-2570
ISSN	0271-6798
DOI	10.1097/BPO.0000000000002632
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Zs.A 1681: Show issues

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Article ; Online: Cardiac defibrillator therapies during the COVID-19 pandemic: how you look provides perspective.

O'Shea, Catherine J / Middeldorp, Melissa E / Campbell, Kevin / Sanders, Prashanthan

European heart journal

2021 Volume 43, Issue 11, Page(s) 1174–1175

MeSH term(s)	COVID-19 ; Defibrillators, Implantable ; Electric Countershock ; Humans ; Pandemics/prevention & control
Language	English
Publishing date	2021-07-23
Publishing country	England
Document type	Editorial ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	603098-1
ISSN	1522-9645 ; 0195-668X
ISSN (online)	1522-9645
ISSN	0195-668X
DOI	10.1093/eurheartj/ehab385
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Zs.A 1563: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Type IV pili facilitated natural competence in Fusobacterium nucleatum.

Sanders, Blake E / Umaña, Ariana / Nguyen, Tam T D / Williams, Kevin J / Yoo, Christopher C / Casasanta, Michael A / Wozniak, Bryce / Slade, Daniel J

Anaerobe

2023 Volume 82, Page(s) 102760

Abstract: Objectives: Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first ... ...

Abstract	Objectives: Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first characterization of Type IV pili facilitated natural competence in Fusobacterium nucleatum, which is a Gram-negative, anaerobic bacterium that participates in a range of infections and diseases including periodontitis, preterm birth, and cancer. Methods: Here we used bioinformatics on multiple Fusobacterium species, as well as molecular genetics to characterize natural competence in strain F. nucleatum subsp. nucleatum ATCC 23726. Results: We bioinformatically identified components of the Type IV conjugal pilus machinery and show this is a conserved system within the Fusobacterium genus. We next validate Type IV pili in natural competence in F. nucleatum ATCC 23726 and show that gene deletions in key components of pilus deployment (pilQ) and cytoplasmic DNA import (comEC) abolish DNA uptake and chromosomal incorporation. We next show that natural competence may require native F. nucleatum DNA methylation to bypass restriction modification systems and allow subsequent genomic homologous recombination. Conclusions: In summary, this proof of principle study provides the first characterization of natural competence in Fusobacterium nucleatum and highlights the potential to exploit this DNA import mechanism as a genetic tool to characterize virulence mechanisms of an opportunistic oral pathogen.
MeSH term(s)	Infant, Newborn ; Humans ; Female ; Fusobacterium nucleatum/metabolism ; Base Composition ; Sequence Analysis, DNA ; Phylogeny ; Premature Birth ; RNA, Ribosomal, 16S ; Fusobacterium ; DNA, Bacterial/genetics ; Fusobacterium Infections/microbiology
Chemical Substances	RNA, Ribosomal, 16S ; DNA, Bacterial
Language	English
Publishing date	2023-07-13
Publishing country	England
Document type	Journal Article
ZDB-ID	1237621-8
ISSN	1095-8274 ; 1075-9964
ISSN (online)	1095-8274
ISSN	1075-9964
DOI	10.1016/j.anaerobe.2023.102760
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Article: Clinical Performance of Cas13a-based Point-of-Care Lateral Flow Assay for Detecting

Allan-Blitz, Lao-Tzu / Sanders, Gabriela / Shah, Palak / Adams, Gordon / Jarolimova, Jana / Ard, Kevin / Branda, John A / Klausner, Jeffrey D / Sabeti, Pardis C / Lemieux, Jacob E

medRxiv : the preprint server for health sciences

2024

Abstract: Background: Diagnosis of : Methods: We collected an additional urine specimen among individuals enrolling in an ongoing clinical trial at the Massachusetts General Hospital Sexual Health Clinic who presented with symptoms of urethritis or cervicitis ( ...

Abstract	Background: Diagnosis of Methods: We collected an additional urine specimen among individuals enrolling in an ongoing clinical trial at the Massachusetts General Hospital Sexual Health Clinic who presented with symptoms of urethritis or cervicitis (urethral or vaginal discharge, dysuria, or dyspareunia). We then assessed thermal, detergent, and combination DNA extraction conditions, varying the duration of heat at 95°C and concentration of Triton X. We assessed the efficacy of the various DNA extraction methods by quantitative polymerase chain reaction (qPCR). Once an extraction method was selected, we incubated samples for 90 minutes to permit isothermal recombinase polymerase amplification. We then assessed the performance of lateral flow Cas13a-based detection using our previously designed Results: We assessed DNA extraction conditions on 3 clinical urine specimens. There was no consistent significant difference in copies per microliter of DNA obtained using more or less heat. On average, we noted that 0.02% triton combined with 5 minutes of heating to 95°C resulted in the highest DNA yield, however, 0.02% triton alone resulted in a quantity of DNA that was above the previously determined analytic sensitivity of the assay. Given that detergent-based extraction is more easily deployable, we selected that as our method for extraction. We treated 23 clinical specimens with 0.02% triton, which we added to the Cas13a detection system. We ran all lateral flow detections in duplicate. The Cas13a-based assay detected 8 of 8 (100%) positive specimens, and 0 of 15 negative specimens. Conclusion: Using point-of-care DNA extraction, isothermal amplification, and Cas13a-based detection, our point-of-care lateral flow
Language	English
Publishing date	2024-03-04
Publishing country	United States
Document type	Preprint
DOI	10.1101/2024.03.01.24303603
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Article ; Online: Type IV pili facilitated natural competence in Fusobacterium nucleatum

Sanders, Blake E. / Umaña, Ariana / Nguyen, Tam T.D. / Williams, Kevin J. / Yoo, Christopher C. / Casasanta, Michael A. / Wozniak, Bryce / Slade, Daniel J.

Anaerobe. 2023 Aug., v. 82 p.102760-

2023

Abstract: Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first characterization of Type IV ...

Abstract	Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first characterization of Type IV pili facilitated natural competence in Fusobacterium nucleatum, which is a Gram-negative, anaerobic bacterium that participates in a range of infections and diseases including periodontitis, preterm birth, and cancer. Here we used bioinformatics on multiple Fusobacterium species, as well as molecular genetics to characterize natural competence in strain F. nucleatum subsp. nucleatum ATCC 23726. We bioinformatically identified components of the Type IV conjugal pilus machinery and show this is a conserved system within the Fusobacterium genus. We next validate Type IV pili in natural competence in F. nucleatum ATCC 23726 and show that gene deletions in key components of pilus deployment (pilQ) and cytoplasmic DNA import (comEC) abolish DNA uptake and chromosomal incorporation. We next show that natural competence may require native F. nucleatum DNA methylation to bypass restriction modification systems and allow subsequent genomic homologous recombination. In summary, this proof of principle study provides the first characterization of natural competence in Fusobacterium nucleatum and highlights the potential to exploit this DNA import mechanism as a genetic tool to characterize virulence mechanisms of an opportunistic oral pathogen.
Keywords	DNA ; DNA methylation ; Fusobacterium nucleatum ; bacteria ; bioinformatics ; chromosomes ; fimbriae ; gene transfer ; genes ; genomics ; homologous recombination ; pathogens ; periodontitis ; premature birth ; virulence ; Fusobacterium ; DNA methyltransferase ; Natural competence ; Type IV pili ; Transformation ; Bacterial genetics
Language	English
Dates of publication	2023-08
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	1237621-8
ISSN	1075-9964
ISSN	1075-9964
DOI	10.1016/j.anaerobe.2023.102760
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 4318: Show issues

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Article ; Online: Coronary Artery Fistulas: A Review of the Current and Future Roles of Imaging.

Kalisz, Kevin / Sanders, Alison E / Avery, Ryan / Allen, Bradley D

Journal of thoracic imaging

2020 Volume 36, Issue 6, Page(s) 333–344

Abstract: Coronary artery fistulas are uncommon but clinically important entities that may produce symptoms and significant complications such as angina, myocardial infarction, coronary artery aneurysm formation, and congestive heart failure. Multiple fistula ... ...

Abstract	Coronary artery fistulas are uncommon but clinically important entities that may produce symptoms and significant complications such as angina, myocardial infarction, coronary artery aneurysm formation, and congestive heart failure. Multiple fistula types have been recognized, and classification uses factors such as etiology, coronary artery origin, and drainage site. Both invasive and noninvasive imaging play an important role in the management and treatment of these patients, and often times, more than one modality is necessary for comprehensive evaluation of coronary fistulas. Recent advances in both functional and anatomic imaging will likely also play a growing role in fistula evaluation. The purpose of this article is to review the classification, pathophysiology, clinical presentations, imaging findings, treatment, and future imaging directions of coronary artery fistulas.
MeSH term(s)	Coronary Angiography ; Coronary Artery Disease/diagnostic imaging ; Coronary Vessel Anomalies ; Fistula ; Humans
Language	English
Publishing date	2020-09-05
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	632900-7
ISSN	1536-0237 ; 0883-5993
ISSN (online)	1536-0237
ISSN	0883-5993
DOI	10.1097/RTI.0000000000000557
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Article ; Online: Enhanced Fusobacterium nucleatum Genetics Using Host DNA Methyltransferases To Bypass Restriction-Modification Systems.

Umaña, Ariana / Nguyen, Tam T D / Sanders, Blake E / Williams, Kevin J / Wozniak, Bryce / Slade, Daniel J

Journal of bacteriology

2022 Volume 204, Issue 12, Page(s) e0027922

Abstract: Bacterial restriction-modification (R-M) systems are a first-line immune defense against foreign DNA from viruses and other bacteria. While R-M systems are critical in maintaining genome integrity, R-M nucleases unfortunately present significant barriers ...

Abstract	Bacterial restriction-modification (R-M) systems are a first-line immune defense against foreign DNA from viruses and other bacteria. While R-M systems are critical in maintaining genome integrity, R-M nucleases unfortunately present significant barriers to targeted genetic modification. Bacteria of the genus
MeSH term(s)	DNA Methylation ; DNA Restriction-Modification Enzymes/genetics ; Fusobacterium nucleatum/genetics ; Methyltransferases/genetics
Chemical Substances	DNA Restriction-Modification Enzymes ; Methyltransferases (EC 2.1.1.-)
Language	English
Publishing date	2022-11-03
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2968-3
ISSN	1098-5530 ; 0021-9193
ISSN (online)	1098-5530
ISSN	0021-9193
DOI	10.1128/jb.00279-22
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Article ; Online: Impact of intensive follow-up of cardiac implantable electronic devices via remote monitoring: A pilot study.

Heath, Kyle / O'Shea, Catherine / Thomas, Gijo / Harper, Curtis / Campbell, Kevin / Sanders, Prashanthan / Middeldorp, Melissa E

Heart rhythm O2

2022 Volume 4, Issue 2, Page(s) 90–96

Abstract: Background: The volume of remote monitoring (RM) data generates a significant workload and is generally dealt with by clinic staff during standard office hours, potentially delaying clinical action.: Objective: The purpose of this study was to ... ...

Abstract	Background: The volume of remote monitoring (RM) data generates a significant workload and is generally dealt with by clinic staff during standard office hours, potentially delaying clinical action. Objective: The purpose of this study was to determine the clinical efficiency and workflow of implementing intensive RM (IRM) in patients with cardiac implantable electronic device (CIED) when compared with standard RM (SRM). Methods: From a cohort of >1500 remotely monitored devices, 70 patients were randomly selected to undergo IRM. For comparison, an equal number of matched patients were prospectively selected for SRM. Intensive follow-up occurred via automated vendor-neutral software with rapid alert processing by International Board of Heart Rhythm Examiners-certified device specialists. Standard follow-up was conducted by clinic staff during office hours via individual device vendor interfaces. Alerts were categorized on the basis of the level of acuity as actionable (red [high], yellow [moderate]), or green [not requiring action]). Results: Over 9 months of follow-up, 922 remote transmissions were received; 339 (36.8%) were coded as actionable alerts (118 in IRM and 221 in SRM; Conclusion: Intensive and managed RM results in a significant reduction in time to review alerts and number of actionable alerts. Monitoring with enhanced alert adjudication is needed to facilitate device clinic efficiency and optimize patient care. Study registration: ACTRN12621001275853.
Language	English
Publishing date	2022-11-17
Publishing country	United States
Document type	Journal Article
ISSN	2666-5018
ISSN (online)	2666-5018
DOI	10.1016/j.hroo.2022.11.002
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Article ; Online: Device-detected atrial fibrillation in a large remote-monitored cohort: implications for anticoagulation and need for new pathways of service delivery.

O'Shea, Catherine J / Brooks, Anthony G / Middeldorp, Melissa E / Harper, Curtis / Hendriks, Jeroen M / Russo, Andrea M / Freeman, James V / Gopinathannair, Rakesh / Varma, Niraj / Deering, Thomas F / Campbell, Kevin / Sanders, Prashanthan

Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing

2023 Volume 66, Issue 7, Page(s) 1659–1668

Abstract: Background: Remote monitoring (RM) can facilitate early detection of subclinical and symptomatic atrial fibrillation (AF), providing an opportunity to evaluate the need for stroke prevention therapies. We aimed to characterize the burden of RM AF alerts ...

Abstract	Background: Remote monitoring (RM) can facilitate early detection of subclinical and symptomatic atrial fibrillation (AF), providing an opportunity to evaluate the need for stroke prevention therapies. We aimed to characterize the burden of RM AF alerts and its impact on anticoagulation of patients with device-detected AF. Methods: Consecutive patients with a cardiac implantable electronic device, at least one AF episode, undergoing RM were included and assigned an estimated minimum CHA Results: From 7651 individual, 389,188 AF episodes were identified, 3120 (40.8%) permanent pacemakers, 2260 (29.5%) implantable loop recorders (ILRs), 987 (12.9%) implantable cardioverter defibrillators, 968 (12.7%) cardiac resynchronization therapy (CRT) defibrillators, and 316 (4.1%) CRT pacemakers. ILRs transmitted 48.8% of all AF episodes. At twelve-months, 3404 (44.5%) AF < 6 min, 1367 (17.9%) 6 min-6 h, 1206 (15.8%) 6-24 h, and 1674 (21.9%) ≥ 24 h. A minimum CHA Conclusions: Despite being intensively managed via RM software system incorporating cues for anticoagulation, a substantial proportion of patients with increased stroke risk remained unanticoagulated after a device-detected AF episode of significant duration. These data highlight the need for improved clinical response pathways and an integrated care approach to RM. Trial registration: Australian New Zealand Clinical Trial Registry: ACTRN12620001232921.
MeSH term(s)	Humans ; Anticoagulants ; Atrial Fibrillation/diagnosis ; Australia ; Defibrillators, Implantable ; Pacemaker, Artificial ; Risk Factors
Chemical Substances	Anticoagulants
Language	English
Publishing date	2023-02-03
Publishing country	Netherlands
Document type	Clinical Trial ; Journal Article
ZDB-ID	1329179-8
ISSN	1572-8595 ; 1383-875X
ISSN (online)	1572-8595
ISSN	1383-875X
DOI	10.1007/s10840-023-01481-4
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