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  1. Article ; Online: The acyl-CoA synthetase

    Charital, Sarah / Shunmugam, Serena / Dass, Sheena / Alazzi, Anna Maria / Arnold, Christophe-Sébastien / Katris, Nicholas J / Duley, Samuel / Quansah, Nyamekye A / Pierrel, Fabien / Govin, Jérôme / Yamaryo-Botté, Yoshiki / Botté, Cyrille Y

    mBio

    2024  Volume 15, Issue 4, Page(s) e0042724

    Abstract: Apicomplexa parasites cause major diseases such as toxoplasmosis and malaria that have major health and economic burdens. These unicellular pathogens are obligate intracellular parasites that heavily depend on lipid metabolism for the survival within ... ...

    Abstract Apicomplexa parasites cause major diseases such as toxoplasmosis and malaria that have major health and economic burdens. These unicellular pathogens are obligate intracellular parasites that heavily depend on lipid metabolism for the survival within their hosts. Their lipid synthesis relies on an essential combination of fatty acids (FAs) obtained from both
    MeSH term(s) Humans ; Toxoplasma/metabolism ; Lipid Metabolism ; Saccharomyces cerevisiae/metabolism ; Toxoplasmosis/parasitology ; Fatty Acids/metabolism ; Nutrients ; Malaria ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism
    Chemical Substances Fatty Acids ; Protozoan Proteins
    Language English
    Publishing date 2024-03-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.00427-24
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  2. Article ; Online: Champignons pathogènes - Un nouvel espoir de traitement des infections généralisées.

    Petosa, Carlo / Govin, Jérôme / Mietton, Flore

    Medecine sciences : M/S

    2018  Volume 34, Issue 2, Page(s) 123–125

    Title translation A new hope to fight invasive fungal infection.
    MeSH term(s) Animals ; Antifungal Agents/therapeutic use ; Candida albicans/drug effects ; Candida albicans/pathogenicity ; High-Throughput Screening Assays/methods ; Humans ; Invasive Fungal Infections/drug therapy ; Invasive Fungal Infections/microbiology ; Molecular Targeted Therapy/methods ; Molecular Targeted Therapy/trends ; Proteins/chemistry
    Chemical Substances Antifungal Agents ; Proteins ; bromodomain and extra-terminal domain protein, human
    Language French
    Publishing date 2018-02-16
    Publishing country France
    Document type News
    ZDB-ID 632733-3
    ISSN 1958-5381 ; 0767-0974
    ISSN (online) 1958-5381
    ISSN 0767-0974
    DOI 10.1051/medsci/20183402007
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2872: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Bromodomains shake the hegemony of pan-acetyl antibodies.

    Champleboux, Morgane / Govin, Jérôme

    Proteomics

    2015  Volume 15, Issue 9, Page(s) 1457–1458

    Abstract: Acetylation signaling pathways are involved in numerous cellular processes and are used as therapeutic targets in several disease contexts. However, acetylated proteins only represent a minor fraction of the full proteome, and the identification and ... ...

    Abstract Acetylation signaling pathways are involved in numerous cellular processes and are used as therapeutic targets in several disease contexts. However, acetylated proteins only represent a minor fraction of the full proteome, and the identification and quantification of acetylated sites remain a technological challenge. Currently, pan-acetyl antibodies are used to increase the abundance of acetylated peptides through affinity purification before MS analysis. These antibodies are powerful reagents, but they are hampered by a lack of specificity, affinity, and batch-to-batch reproducibility. In this issue, Bryson et al. (Proteomics 2015 15, 1470-1475) present an interesting alternative to these antibodies, in the form of bromodomains. These domains specifically recognize acetylated lysines, and were successfully used in this study to enrich for acetylated peptides before MS analysis. Future development of this pioneering approach could help overcome this limiting step in the characterization of acetylproteomes.
    MeSH term(s) Acetylation ; Antibodies/chemistry ; Humans ; Lysine/analysis ; Mass Spectrometry/methods ; Protein Structure, Tertiary ; Proteome/chemistry ; Proteomics/methods
    Chemical Substances Antibodies ; Proteome ; Lysine (K3Z4F929H6)
    Language English
    Publishing date 2015-05
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2032093-0
    ISSN 1615-9861 ; 1615-9853
    ISSN (online) 1615-9861
    ISSN 1615-9853
    DOI 10.1002/pmic.201500116
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5386: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.A 1868: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Proteomic Analysis of Histone Variants and Their PTMs: Strategies and Pitfalls.

    El Kennani, Sara / Crespo, Marion / Govin, Jérôme / Pflieger, Delphine

    Proteomes

    2018  Volume 6, Issue 3

    Abstract: Epigenetic modifications contribute to the determination of cell fate and differentiation. The molecular mechanisms underlying histone variants and post-translational modifications (PTMs) have been studied in the contexts of development, differentiation, ...

    Abstract Epigenetic modifications contribute to the determination of cell fate and differentiation. The molecular mechanisms underlying histone variants and post-translational modifications (PTMs) have been studied in the contexts of development, differentiation, and disease. Antibody-based assays have classically been used to target PTMs, but these approaches fail to reveal combinatorial patterns of modifications. In addition, some histone variants are so similar to canonical histones that antibodies have difficulty distinguishing between these isoforms. Mass spectrometry (MS) has progressively developed as a powerful technology for the study of histone variants and their PTMs. Indeed, MS analyses highlighted exquisitely complex combinations of PTMs, suggesting “crosstalk” between them, and also revealed that PTM patterns are often variant-specific. Even though the sensitivity and acquisition speed of MS instruments have considerably increased alongside the development of computational tools for the study of multiple PTMs, it remains challenging to correctly describe the landscape of histone PTMs, and in particular to confidently assign modifications to specific amino acids. Here, we provide an inventory of MS-based strategies and of the pitfalls inherent to histone PTM and variant characterization, while stressing the complex interplay between PTMs and histone sequence variations. We will particularly illustrate the roles played by MS-based analyses in identifying and quantifying histone variants and modifications.
    Language English
    Publishing date 2018-06-21
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2720995-7
    ISSN 2227-7382
    ISSN 2227-7382
    DOI 10.3390/proteomes6030029
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  5. Article ; Online: Histone variants and sensing of chromatin functional states.

    Govin, Jérôme / Khochbin, Saadi

    Nucleus (Austin, Tex.)

    2013  Volume 4, Issue 6, Page(s) 438–442

    Abstract: An extreme case of chromatin remodelling is the genome-wide exchange of histones with basic non-histone DNA-packaging proteins that occurs in post-meiotic male germ cells. The scale of this genome reorganization is such that chromatin needs to undergo a ... ...

    Abstract An extreme case of chromatin remodelling is the genome-wide exchange of histones with basic non-histone DNA-packaging proteins that occurs in post-meiotic male germ cells. The scale of this genome reorganization is such that chromatin needs to undergo a prior "preparation" for a facilitated action of the factors involved. Stage-specific incorporation of specialized histone variants, affecting large domains of chromatin, combined with histone post-translational modifications accompany the successive steps of the male genome reorganization. Recently, it has been shown that a testis-specific H2B variant, TH2B, one of the first identified core histone variants, replaces H2B at the time of cells' commitment into meiotic divisions and contributes to the process of global histone removal. These investigations also revealed a previously unknown histone dosage compensation mechanism that also ensures a functional interconnection between histone variant expression and histone post-translational modifications and will be further discussed here.
    MeSH term(s) Animals ; Chromatin/metabolism ; Female ; Histones/metabolism ; Male ; Protamines/metabolism
    Chemical Substances Chromatin ; Histones ; Protamines
    Language English
    Publishing date 2013-11-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 2619626-8
    ISSN 1949-1042 ; 1949-1034
    ISSN (online) 1949-1042
    ISSN 1949-1034
    DOI 10.4161/nucl.27088
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Identification of IQCH as a calmodulin-associated protein required for sperm motility in humans.

    Cavarocchi, Emma / Sayou, Camille / Lorès, Patrick / Cazin, Caroline / Stouvenel, Laurence / El Khouri, Elma / Coutton, Charles / Kherraf, Zine-Eddine / Patrat, Catherine / Govin, Jérôme / Thierry-Mieg, Nicolas / Whitfield, Marjorie / Ray, Pierre F / Dulioust, Emmanuel / Touré, Aminata

    iScience

    2023  Volume 26, Issue 8, Page(s) 107354

    Abstract: Sperm fertilization ability mainly relies on proper sperm progression through the female genital tract and capacitation, which involves phosphorylation signaling pathways triggered by calcium and bicarbonate. We performed exome sequencing of an infertile ...

    Abstract Sperm fertilization ability mainly relies on proper sperm progression through the female genital tract and capacitation, which involves phosphorylation signaling pathways triggered by calcium and bicarbonate. We performed exome sequencing of an infertile asthenozoospermic patient and identified truncating variants in
    Language English
    Publishing date 2023-07-10
    Publishing country United States
    Document type Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2023.107354
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  7. Article: Histone variants and sensing of chromatin functional states

    Govin, Jérôme / Khochbin, Saadi

    Nucleus. 2013 Nov. 1, v. 4, no. 6

    2013  

    Abstract: An extreme case of chromatin remodelling is the genome-wide exchange of histones with basic non-histone DNA-packaging proteins that occurs in post-meiotic male germ cells. The scale of this genome reorganization is such that chromatin needs to undergo a ... ...

    Abstract An extreme case of chromatin remodelling is the genome-wide exchange of histones with basic non-histone DNA-packaging proteins that occurs in post-meiotic male germ cells. The scale of this genome reorganization is such that chromatin needs to undergo a prior “preparation” for a facilitated action of the factors involved. Stage-specific incorporation of specialized histone variants, affecting large domains of chromatin, combined with histone post-translational modifications accompany the successive steps of the male genome reorganization. Recently, it has been shown that a testis-specific H2B variant, TH2B, one of the first identified core histone variants, replaces H2B at the time of cells’ commitment into meiotic divisions and contributes to the process of global histone removal. These investigations also revealed a previously unknown histone dosage compensation mechanism that also ensures a functional interconnection between histone variant expression and histone post-translational modifications and will be further discussed here.
    Keywords chromatin ; genome ; histones ; males ; meiosis
    Language English
    Dates of publication 2013-1101
    Size p. 438-442.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 2619626-8
    ISSN 1949-1042 ; 1949-1034
    ISSN (online) 1949-1042
    ISSN 1949-1034
    DOI 10.4161/nucl.27088
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  8. Article ; Online: Enantioselective Approach for Expanding the Three-Dimensional Space of Tetrahydroquinoline to Develop BET Bromodomain Inhibitors.

    Lespinasse, Marie-Ange / Wei, Kaiyao / Perrin, Justine / Winkler, Matthias / Hamaidia, Sieme / Leroy, Alexis / Macek Jilkova, Zuzana / Philouze, Christian / Marche, Patrice N / Petosa, Carlo / Govin, Jérôme / Emadali, Anouk / Wong, Yung-Sing

    Chemistry (Weinheim an der Bergstrasse, Germany)

    2022  Volume 28, Issue 64, Page(s) e202202293

    Abstract: The pharmaceutical industry has a pervasive need for chiral specific molecules with optimal affinity for their biological targets. However, the mass production of such compounds is currently limited by conventional chemical routes, that are costly and ... ...

    Abstract The pharmaceutical industry has a pervasive need for chiral specific molecules with optimal affinity for their biological targets. However, the mass production of such compounds is currently limited by conventional chemical routes, that are costly and have an environmental impact. Here, we propose an easy access to obtain new tetrahydroquinolines, a motif found in many bioactive compounds, that is rapid and cost effective. Starting from simple raw materials, the procedure uses a proline-catalyzed Mannich reaction followed by the addition of BF
    MeSH term(s) Stereoisomerism ; Quinolines ; Antineoplastic Agents ; Catalysis
    Chemical Substances 1,2,3,4-tetrahydroquinoline (CCR50N1Z9G) ; Quinolines ; Antineoplastic Agents
    Language English
    Publishing date 2022-09-13
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1478547-X
    ISSN 1521-3765 ; 0947-6539
    ISSN (online) 1521-3765
    ISSN 0947-6539
    DOI 10.1002/chem.202202293
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  9. Article ; Online: Toward more potent imidazopyridine inhibitors of Candida albicans Bdf1: Modeling the role of structural waters in selective ligand binding.

    Zhou, Yingsheng / Overhulse, Justin M / Dupper, Nathan J / Guo, Yanchun / Kashemirov, Boris A / Wei, Kaiyao / Govin, Jérôme / Petosa, Carlo / McKenna, Charles E

    Journal of computational chemistry

    2022  Volume 43, Issue 32, Page(s) 2121–2130

    Abstract: Novel agents to treat invasive fungal infections are urgently needed because the small number of established targets in pathogenic fungi makes the existing drug repertoire particularly vulnerable to the emergence of resistant strains. Recently, we ... ...

    Abstract Novel agents to treat invasive fungal infections are urgently needed because the small number of established targets in pathogenic fungi makes the existing drug repertoire particularly vulnerable to the emergence of resistant strains. Recently, we reported that Candida albicans Bdf1, a bromodomain and extra-terminal domain (BET) bromodomain with paired acetyl-lysine (AcK) binding sites (BD1 and BD2) is essential for fungal cell growth and that an imidazopyridine (1) binds to BD2 with selectivity versus both BD1 and human BET bromodomains. Bromodomain binding pockets contain a conserved array of structural waters. Molecular dynamics simulations now reveal that one water molecule is less tightly bound to BD2 than to BD1, explaining the site selectivity of 1. This insight is useful in the performance of ligand docking studies to guide design of more effective Bdf1 inhibitors, as illustrated by the design of 10 new imidazopyridine BD2 ligands 1a-j, for which experimental binding and site selectivity data are presented.
    MeSH term(s) Humans ; Candida albicans/metabolism ; Ligands ; Transcription Factors/metabolism ; Binding Sites
    Chemical Substances imidazopyridine ; Ligands ; Transcription Factors
    Language English
    Publishing date 2022-10-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1479181-X
    ISSN 1096-987X ; 0192-8651
    ISSN (online) 1096-987X
    ISSN 0192-8651
    DOI 10.1002/jcc.26997
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  10. Article ; Online: Characterization of Post-Meiotic Male Germ Cell Genome Organizational States.

    Govin, Jérôme / Barral, Sophie / Morozumi, Yuichi / Hoghoughi, Naghmeh / Buchou, Thierry / Rousseaux, Sophie / Khochbin, Saadi

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1832, Page(s) 293–307

    Abstract: Dramatic and unique genome reorganizations accompany the differentiation of haploid male germ cells, characterized by a gradual loss of the vast majority of histones leading to a final tight compaction of the genome by protamines. Despite being essential ...

    Abstract Dramatic and unique genome reorganizations accompany the differentiation of haploid male germ cells, characterized by a gradual loss of the vast majority of histones leading to a final tight compaction of the genome by protamines. Despite being essential for procreation and the life cycle, the mechanisms driving the transformation of nucleosomes into nucleoprotamines remain poorly understood. To address this issue, our laboratory has developed a number of specific approaches, ranging from the purification of spermatogenic cells at specific stages, the analysis of chromatin transitional states, the functional characterization of histone variants, histone-replacing proteins and their chaperones. This chapter will detail all related relevant techniques with a particular emphasis on methods allowing the functional studies of histone variants and the genome organizational states associated with the studied histones in spermatogenic cells undergoing histone-to-protamine exchange.
    MeSH term(s) Animals ; Genome ; Germ Cells/cytology ; Germ Cells/metabolism ; Histones/metabolism ; Male ; Meiosis/genetics ; Mice ; Micrococcal Nuclease/metabolism ; Nuclear Proteins/isolation & purification ; Nucleosomes/metabolism ; Proteomics ; Solubility ; Spermatids/cytology ; Spermatids/metabolism
    Chemical Substances Histones ; Nuclear Proteins ; Nucleosomes ; Micrococcal Nuclease (EC 3.1.31.1)
    Language English
    Publishing date 2018-07-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-8663-7_16
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