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Article ; Online: Frequencies of SARS-CoV-2 Spike Protein-Specific Memory B Cells in Human PBMCs, Quantified by ELISPOT Assay.

Swanson, Ilya M / Haralambieva, Iana H / Rasche, Madeleine M / Ovsyannikova, Inna G / Kennedy, Richard B

Methods in molecular biology (Clifton, N.J.)

2024 Volume 2768, Page(s) 153–166

Abstract: Vaccination against SARS-CoV-2 with coronavirus vaccines that elicit protective immune responses is critical to the prevention of severe disease and mortality associated with SARS-CoV-2 infection. Understanding the adaptive immune responses to SARS-CoV-2 ...

Abstract	Vaccination against SARS-CoV-2 with coronavirus vaccines that elicit protective immune responses is critical to the prevention of severe disease and mortality associated with SARS-CoV-2 infection. Understanding the adaptive immune responses to SARS-CoV-2 infection and/or vaccination will continue to aid in the development of next-generation vaccines. Studies have shown the important role of SARS-CoV-2-specific antibodies for both disease resolution and prevention of COVID-19 serious sequelae following vaccination. However, antibody responses are short-lived, highlighting the importance of studying antigen-specific B-cell responses to better understand durable immunity and immunologic memory. Since the spike protein is the main target of antibody-producing B cells, we developed a SARS-CoV-2 memory B cell ELISPOT assay to measure the frequencies of spike-specific B cells after COVID-19 infection and/or vaccination. Here, we describe in detail the methodology for using this ELISPOT assay to quantify SARS-CoV-2 spike-specific memory B cells produced by infection and/or vaccination in human PBMC samples. Application of this assay may help better understand and predict SARS-CoV-2 recall immune responses and to develop potential B cell correlates of protection at the methodological level.
MeSH term(s)	Humans ; Memory B Cells ; Spike Glycoprotein, Coronavirus ; Enzyme-Linked Immunospot Assay ; Leukocytes, Mononuclear ; COVID-19 ; SARS-CoV-2 ; Antibodies, Viral ; Vaccination ; Vaccines
Chemical Substances	spike protein, SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Antibodies, Viral ; Vaccines
Language	English
Publishing date	2024-03-19
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3690-9_10
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Article ; Online: Pharmacogenomics and Vaccine Development.

Poland, Gregory A / Ovsyannikova, Inna G / Kennedy, Richard B

Clinical pharmacology and therapeutics

2021 Volume 110, Issue 3, Page(s) 546–548

MeSH term(s)	Animals ; Drug Development ; Humans ; Pharmacogenetics ; Proteomics ; Systems Biology ; Vaccines/genetics
Chemical Substances	Vaccines
Language	English
Publishing date	2021-06-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	123793-7
ISSN	1532-6535 ; 0009-9236
ISSN (online)	1532-6535
ISSN	0009-9236
DOI	10.1002/cpt.2288
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Zs.A 20: Show issues

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Article ; Online: Early B cell transcriptomic markers of measles-specific humoral immunity following a 3

Haralambieva, Iana H / Chen, Jun / Quach, Huy Quang / Ratishvili, Tamar / Warner, Nathaniel D / Ovsyannikova, Inna G / Poland, Gregory A / Kennedy, Richard B

Frontiers in immunology

2024 Volume 15, Page(s) 1358477

Abstract: B cell transcriptomic signatures hold promise for the early prediction of vaccine-induced humoral immunity and vaccine protective efficacy. We performed a longitudinal study in 232 healthy adult participants before/after a ... ...

Abstract	B cell transcriptomic signatures hold promise for the early prediction of vaccine-induced humoral immunity and vaccine protective efficacy. We performed a longitudinal study in 232 healthy adult participants before/after a 3
MeSH term(s)	Adult ; Humans ; Measles-Mumps-Rubella Vaccine ; Immunity, Humoral ; Longitudinal Studies ; Antibodies, Viral ; Measles ; Gene Expression Profiling ; Nerve Tissue Proteins
Chemical Substances	Measles-Mumps-Rubella Vaccine ; Antibodies, Viral ; BEX2 protein, human ; Nerve Tissue Proteins
Language	English
Publishing date	2024-04-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2024.1358477
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Article ; Online: The need for broadly protective COVID-19 vaccines: Beyond S-only approaches.

Poland, Gregory A / Ovsyannikova, Inna G / Kennedy, Richard B

Vaccine

2021 Volume 39, Issue 31, Page(s) 4239–4241

MeSH term(s)	COVID-19 ; COVID-19 Vaccines ; Epitopes, B-Lymphocyte ; Epitopes, T-Lymphocyte ; Humans ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Vaccines, Subunit ; Viral Vaccines
Chemical Substances	COVID-19 Vaccines ; Epitopes, B-Lymphocyte ; Epitopes, T-Lymphocyte ; Spike Glycoprotein, Coronavirus ; Vaccines, Subunit ; Viral Vaccines
Language	English
Publishing date	2021-06-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2021.06.028
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Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 458: Show issues

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Article: Polygenic Prediction of Cellular Immune Responses to Mumps Vaccine.

Coombes, Brandon J / Ovsyannikova, Inna G / Schaid, Daniel J / Warner, Nathaniel D / Poland, Gregory A / Kennedy, Richard B

medRxiv : the preprint server for health sciences

2024

Abstract: In this report, we provide a follow-up analysis of a previously published genome-wide association study of host genetic variants associated with inter-individual variations in cellular immune responses to mumps vaccine. Here we report the results of a ... ...

Abstract	In this report, we provide a follow-up analysis of a previously published genome-wide association study of host genetic variants associated with inter-individual variations in cellular immune responses to mumps vaccine. Here we report the results of a polygenic score (PGS) analysis showing how common variants can predict mumps vaccine response. We found higher PGS for IFNγ, IL-2, and TNFα were predictive of higher post-vaccine IFNγ (p-value = 2e-6), IL-2 (p = 2e-7), and TNFα (p = 0.004) levels, respectively. Control of immune responses after vaccination is complex and polygenic in nature. Our results suggest that the PGS-based approach enables better capture of the combined genetic effects that contribute to mumps vaccine-induced immunity, potentially offering a more comprehensive understanding than traditional single-variant GWAS. This approach will likely have broad utility in studying genetic control of immune responses to other vaccines and to infectious diseases.
Language	English
Publishing date	2024-02-27
Publishing country	United States
Document type	Preprint
DOI	10.1101/2024.02.23.24303277
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Article ; Online: Restricted Omicron-specific cross-variant memory B-cell immunity after a 3rd dose/booster of monovalent Wuhan-Hu-1-containing COVID-19 mRNA vaccine.

Haralambieva, Iana H / Monroe, Jonathon M / Ovsyannikova, Inna G / Warner, Nathaniel D / Grill, Diane E / Poland, Gregory A / Kennedy, Richard B

Vaccine

2024 Volume 42, Issue 4, Page(s) 912–917

Abstract: ... divergent SARS-CoV-2 variants (e.g., Omicron) is understudied. In this longitudinal study subjects receiving ...

Abstract	The responsiveness/cross-binding of vaccine-induced memory B cells/MBCs to previous and emerging divergent SARS-CoV-2 variants (e.g., Omicron) is understudied. In this longitudinal study subjects receiving two or three doses of monovalent ancestral strain-containing COVID-19 mRNA vaccine were evaluated. In contrast to others, we observed significantly lower frequencies of MBCs reactive to the receptor-binding domain/RBD, the N-terminal domain/NTD, and the S1 of Omicron/BA.1, compared to Wuhan and Delta, even after a 3rd vaccine dose/booster. Our study is a proof of concept that MBC cross-reactivity to variants with greater sequence divergence from the vaccine strain may be overestimated and suggests that these variants may exhibit immune escape with reduced recognition by circulating pre-existing MBCs upon infection.
MeSH term(s)	Humans ; COVID-19 Vaccines ; Longitudinal Studies ; Memory B Cells ; mRNA Vaccines ; COVID-19/prevention & control ; SARS-CoV-2 ; Antibodies, Neutralizing ; Antibodies, Viral
Chemical Substances	COVID-19 Vaccines ; mRNA Vaccines ; Antibodies, Neutralizing ; Antibodies, Viral
Language	English
Publishing date	2024-01-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2024.01.032
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Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 458: Show issues

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Article ; Online: Detection of SARS-CoV-2-Specific Cells Utilizing Whole Proteins and/or Peptides in Human PBMCs Using IFN-ƴ ELISPOT Assay.

Rasche, Madeleine M / Kaufmann, Ella C / Ratishvili, Tamar / Swanson, Ilya M / Ovsyannikova, Inna G / Kennedy, Richard B

Methods in molecular biology (Clifton, N.J.)

2024 Volume 2768, Page(s) 117–133

Abstract: SARS-CoV-2 continues to threaten global public health, making COVID-19 immunity studies of utmost importance. Waning of antibody responses postinfection and/or vaccination and the emergence of immune escape variants have been ongoing challenges in ... ...

Abstract	SARS-CoV-2 continues to threaten global public health, making COVID-19 immunity studies of utmost importance. Waning of antibody responses postinfection and/or vaccination and the emergence of immune escape variants have been ongoing challenges in mitigating SARS-CoV-2 morbidity and mortality. While a tremendous amount of work has been done to characterize humoral immune responses to SARS-CoV-2 virus and vaccines, cellular immunity, mediated by T cells, is critical for efficient viral control and protection and demonstrates high durability and cross-reactivity to coronavirus variants. Thus, ELISPOT, a standard assay for antigen-specific cellular immune response assessment, allows us to evaluate SARS-CoV-2-specific T-cell response by quantifying the frequency of SARS-CoV-2-specific cytokine-secreting cells in vitro. We have outlined a detailed procedure to study T-cell recall responses to SARS-CoV-2 in human peripheral blood mononuclear cells (PBMCs) following infection and/or vaccination using an optimized IFN-γ ELISPOT assay. Our methodologies can be adapted to assess other cytokines and are a useful tool for studying other viral pathogen and/or peptide-specific T-cell responses.
MeSH term(s)	Humans ; SARS-CoV-2 ; Enzyme-Linked Immunospot Assay ; Leukocytes, Mononuclear ; COVID-19 ; Peptides ; Cytokines ; Immunity, Cellular ; Antibodies, Viral ; Vaccination
Chemical Substances	Peptides ; Cytokines ; Antibodies, Viral
Language	English
Publishing date	2024-03-19
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3690-9_8
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Article ; Online: Update on Influenza Vaccines: Needs and Progress.

Kennedy, Richard B / Ovsyannikova, Inna G / Poland, Gregory A

The journal of allergy and clinical immunology. In practice

2021 Volume 9, Issue 10, Page(s) 3599–3603

Abstract: Influenza is an annual seasonal epidemic, and occasionally pandemic, respiratory disease that causes considerable morbidity and mortality worldwide. Despite the widespread availability of safe and effective vaccines since the 1950s, this virus continues ... ...

Abstract	Influenza is an annual seasonal epidemic, and occasionally pandemic, respiratory disease that causes considerable morbidity and mortality worldwide. Despite the widespread availability of safe and effective vaccines since the 1950s, this virus continues to pose a significant public health threat. Variable and often weak vaccine effectiveness, antigenic drift and shift, and vaccine hesitancy are some of the obstacles that must be overcome to control this disease. In this article, we briefly review current influenza vaccines, address safety concerns and the need for newer influenza vaccines of higher efficacy, and discuss efforts to create broadly protective, universal influenza vaccines.
MeSH term(s)	Humans ; Influenza Vaccines ; Influenza, Human/epidemiology ; Influenza, Human/prevention & control ; Pandemics
Chemical Substances	Influenza Vaccines
Language	English
Publishing date	2021-08-18
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2843237-X
ISSN	2213-2201 ; 2213-2198
ISSN (online)	2213-2201
ISSN	2213-2198
DOI	10.1016/j.jaip.2021.08.003
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Article ; Online: Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA.

Quach, Huy Quang / Ovsyannikova, Inna G / Poland, Gregory A / Kennedy, Richard B

Journal of immunological methods

2022 Volume 505, Page(s) 113275

Abstract: Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to ... ...

Abstract	Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters.
MeSH term(s)	Animals ; Antibodies, Viral ; COVID-19 ; Cricetinae ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; Mesocricetus ; Peptides ; SARS-CoV-2
Chemical Substances	Antibodies, Viral ; Peptides
Language	English
Publishing date	2022-04-16
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120142-6
ISSN	1872-7905 ; 0022-1759
ISSN (online)	1872-7905
ISSN	0022-1759
DOI	10.1016/j.jim.2022.113275
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Article ; Online: Evaluating immunogenicity of pathogen-derived T-cell epitopes to design a peptide-based smallpox vaccine.

Quach, Huy Quang / Ovsyannikova, Inna G / Poland, Gregory A / Kennedy, Richard B

Scientific reports

2022 Volume 12, Issue 1, Page(s) 15401

Abstract: Despite the eradication in 1980, developing safe and effective smallpox vaccines remains an active area of research due to the recent outbreaks and the public health concern that smallpox viruses could be used as bioterrorism weapons. Identifying ... ...

Abstract	Despite the eradication in 1980, developing safe and effective smallpox vaccines remains an active area of research due to the recent outbreaks and the public health concern that smallpox viruses could be used as bioterrorism weapons. Identifying immunogenic peptides (epitopes) would create a foundation for the development of a robust peptide-based vaccine. We previously identified a library of naturally-processed, human leukocyte antigen class I-presented vaccinia-derived peptides from infected B cells. In the current study, we evaluated the immunogenicity of these T-cell peptides in both transgenic mouse models and human peripheral blood mononuclear cells. A vaccine based on four selected peptides provided 100% protection against a lethal viral challenge. In addition, responses from memory T cells remained unchanged up to five months. Our results validate a practical approach for identifying and verifying immunogenic peptides for vaccine development and highlight the potential of peptide-based vaccines for various infectious diseases.
MeSH term(s)	Animals ; Epitopes, T-Lymphocyte ; Humans ; Leukocytes, Mononuclear ; Mice ; Peptides ; Smallpox/prevention & control ; Smallpox Vaccine ; Vaccines, Subunit ; Variola virus
Chemical Substances	Epitopes, T-Lymphocyte ; Peptides ; Smallpox Vaccine ; Vaccines, Subunit
Language	English
Publishing date	2022-09-13
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-19679-3
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