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Article ; Online: Mixture analysis of associations between exposure to low levels of multiple metals and semen quality and sperm DNA integrity.

Jeng, Hueiwang Anna / Sikdar, Sinjini / Huang, Yeou-Lih / Pan, Chih-Hon

Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering

2022 Volume 57, Issue 4, Page(s) 318–326

Abstract: The objective of this study was to assess the association of exposure to metal mixtures with semen quality and sperm DNA integrity of coke oven workers ( ...

Abstract	The objective of this study was to assess the association of exposure to metal mixtures with semen quality and sperm DNA integrity of coke oven workers (
MeSH term(s)	8-Hydroxy-2'-Deoxyguanosine ; Bayes Theorem ; Cadmium/analysis ; DNA ; Humans ; Male ; Metals/analysis ; Semen ; Semen Analysis ; Sperm Motility ; Spermatozoa
Chemical Substances	Metals ; Cadmium (00BH33GNGH) ; 8-Hydroxy-2'-Deoxyguanosine (88847-89-6) ; DNA (9007-49-2)
Language	English
Publishing date	2022-04-07
Publishing country	England
Document type	Journal Article
ZDB-ID	196584-0
ISSN	1532-4117 ; 0360-1226 ; 1077-1204 ; 1093-4529
ISSN (online)	1532-4117
ISSN	0360-1226 ; 1077-1204 ; 1093-4529
DOI	10.1080/10934529.2022.2061256
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Article: Mixture analysis of associations between exposure to low levels of multiple metals and semen quality and sperm DNA integrity

Jeng, Hueiwang Anna / Sikdar, Sinjini / Huang, Yeou-Lih / Pan, Chih-Hon

Journal of environmental science and health. 2022 Mar. 21, v. 57, no. 4

2022

Abstract: The objective of this study was to assess the association of exposure to metal mixtures with semen quality and sperm DNA integrity of coke oven workers (n = 96). Urinary six metals (cadmium, lead, arsenic, zinc, selenium, and copper) were quantified ... ...

Abstract	The objective of this study was to assess the association of exposure to metal mixtures with semen quality and sperm DNA integrity of coke oven workers (n = 96). Urinary six metals (cadmium, lead, arsenic, zinc, selenium, and copper) were quantified using inductively coupled-mass spectrometry. Semen quality parameters included sperm concentration, sperm concentration, sperm motility, sperm morphology, and sperm viability. Sperm DNA fragmentation and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) adducts served as biomarkers for assessing sperm DNA integrity. Bayesian kernel machine regression with the hierarchical variable selection process was used for analyzing both individual and joint effects of the metal mixture on the outcomes of semen samples, while adjusting for age, smoking, alcohol consumption, job length, and body mass index. The metal mixture was associated with reduced sperm concentration, motility, viability, and normal morphology. It was novel that a dose-response relationship was observed between exposure of the metal mixture and semen quality. Among the metals tested, cadmium had a reverse relationship with sperm motility, viability, and normal morphology, and a non-linear relationship with sperm viability and sperm motility. The metal mixture and individual metals were not associated with sperm DNA fragmentation and 8-oxodGuo. In conclusion, exposure to metal mixtures and cadmium may exert an association with semen quality and had no association with sperm DNA breakages.
Keywords	Bayesian theory ; DNA ; DNA fragmentation ; alcohol drinking ; arsenic ; biomarkers ; body mass index ; cadmium ; copper ; dose response ; environmental science ; lead ; ovens ; selenium ; semen quality ; spectroscopy ; sperm concentration ; sperm motility ; spermatozoa ; viability ; zinc
Language	English
Dates of publication	2022-0321
Size	p. 318-326.
Publishing place	Taylor & Francis
Document type	Article
ZDB-ID	196584-0
ISSN	1532-4117 ; 0360-1226 ; 1077-1204 ; 1093-4529
ISSN (online)	1532-4117
ISSN	0360-1226 ; 1077-1204 ; 1093-4529
DOI	10.1080/10934529.2022.2061256
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: What do patients think about palliative care? A national survey of hematopoietic stem cell transplant recipients.

Barata, Anna / Abrams, Hannah R / Meyer, Christa / Mau, Lih-Wen / Mattila, Deborah / Burns, Linda J / Ullrich, Christina / Murthy, Hemant / Wood, William A / Petersdorf, Effie / LeBlanc, Thomas W / El-Jawahri, Areej

Blood advances

2023 Volume 7, Issue 10, Page(s) 2032–2041

Abstract: Palliative care (PC) benefits patients undergoing hematopoietic stem cell transplantation (HSCT), but it remains underutilized. Although transplant physicians report concerns regarding how patients perceive PC, HSCT recipients' perceptions about PC ... ...

Abstract	Palliative care (PC) benefits patients undergoing hematopoietic stem cell transplantation (HSCT), but it remains underutilized. Although transplant physicians report concerns regarding how patients perceive PC, HSCT recipients' perceptions about PC remain unaddressed. We conducted a multisite, cross-sectional survey of autologous and allogeneic HSCT recipients 3 to 12 months after transplant to assess their familiarity, knowledge, and perception of PC, as well as their unmet PC needs. We computed a composite score of patients' perceptions of PC and used a generalized linear regression model to examine factors associated with these perceptions. We enrolled 69.6% (250/359) of potential participants (median age = 58.1; 63.1% autologous HSCT). Overall, 44.3.8% (109/249) reported limited knowledge about PC and 52% (127/245) endorsed familiarity with PC. Most patients felt hopeful (54%) and reassured (50%) when they heard the term PC; 83% saw referral as a sign their doctor cared about what was happening to them. In multivariate analyses, patients who were more knowledgeable about PC were more likely to have positive perceptions of PC (B = 7.54, standard error = 1.61, P < .001). Patients' demographics, HSCT features, quality of life, and symptom burden were not significantly associated with perceptions of PC. HSCT recipients have positive perceptions of PC, though many have limited knowledge about its role. Patients who were more knowledgeable about PC were more likely to have positive perceptions of PC. These data do not support transplant physicians' negative concerns about how patients perceive PC and underscore the need to further educate patients and transplant physicians about PC.
MeSH term(s)	Humans ; Middle Aged ; Palliative Care ; Cross-Sectional Studies ; Quality of Life ; Transplantation, Autologous ; Hematopoietic Stem Cell Transplantation
Language	English
Publishing date	2023-03-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2915908-8
ISSN	2473-9537 ; 2473-9529
ISSN (online)	2473-9537
ISSN	2473-9529
DOI	10.1182/bloodadvances.2023009712
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Article: Applying a multi-faceted infection control strategy to improve hospital environmental cleaning quality.

Lin, Hsin-An / Lin, Hsin-Chung / Chen, Lih-Chyang / Huang, Kuo-Yang / Guo, Jong-Long

Heliyon

2024 Volume 10, Issue 3, Page(s) e24928

Abstract: Background: Along with existing infection control policies, repeated education and training of environmental service workers (ESWs) improves their compliance and ultimately reduces hospital-associated infection (HAI) rates. However, only limited studies ...

Abstract	Background: Along with existing infection control policies, repeated education and training of environmental service workers (ESWs) improves their compliance and ultimately reduces hospital-associated infection (HAI) rates. However, only limited studies have explored the health behavioral determinants of ESWs regarding their cleaning performance after implementing an educational intervention with multi-faceted infection control strategy. Objective: To determine whether an educational intervention with multi-faceted infection control strategy improves the health behavioral determinants associated with ESWs' cleaning performance. Methods: Twenty-eight ESWs who received an educational intervention with multi-faceted hospital infection control strategy were included. ESWs' knowledge, perceived benefits and barriers, self-efficacy, health literacy, and cleaning performance were evaluated at pre-intervention, post-intervention, and 3-month follow-up. Results: HAI-related adenosine triphosphate (ATP) levels decreased significantly at post-intervention and 3-month follow-up compared with pre-intervention levels (all p < 0.05). All post-intervention ATP levels met the standard criterion after the 2nd environmental cleaning, with a median score of 267 (range, 71-386). High baseline ATP levels (odds ratio [OR] = 4.195, 95%CI 2.500-7.042, p < 0.05) were positively associated with qualified post-intervention ATP levels, while high education (OR = 0.480, 95%CI 0.276-0.833, p < 0.05) and high baseline knowledge scores (OR = 0.481, 95%CI 0.257-0.903, p = 0.023) were negatively associated with qualified post-intervention ATP levels. Conclusion: Educational intervention using a multi-faceted infection control strategy improves health behavioral determinants (baseline education, knowledge scores and ATP levels) associated with ESWs' hospital cleaning performance. Receiving an educational intervention may increase HAI knowledge of environmental cleaning among ESWs with high education or low baseline HAI knowledge.
Language	English
Publishing date	2024-01-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e24928
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Article ; Online: Growth differentiation factor 15 induces cisplatin resistance through upregulation of xCT expression and glutathione synthesis in gastric cancer.

Wang, Sheng-Fan / Chang, Yuh-Lih / Fang, Wen-Liang / Li, Anna Fen-Yau / Chen, Chian-Feng / Yeh, Tien-Shun / Hung, Giun-Yi / Huang, Kuo-Hung / Lee, Hsin-Chen

Cancer science

2023 Volume 114, Issue 8, Page(s) 3301–3317

Abstract: Gastric cancer is a common cancer worldwide, particularly in East Asia. Chemotherapy is used in adjuvant or palliative therapies for gastric cancer. However, subsequent chemoresistance often develops. Growth differentiation factor 15 (GDF15) links to ... ...

Abstract	Gastric cancer is a common cancer worldwide, particularly in East Asia. Chemotherapy is used in adjuvant or palliative therapies for gastric cancer. However, subsequent chemoresistance often develops. Growth differentiation factor 15 (GDF15) links to several cancers, but its effect on chemoresistance in gastric cancer remains unclear. Here, we analyzed clinical samples from genetic databases and included patients with gastric cancer. We dissected the regulatory mechanism underlying GDF15-mediated resistance of cisplatin in human gastric cancer cells. We showed that GDF15 serum levels might be a valuable biomarker for predicting prognosis in gastric cancer. The expressions of GDF15 and its receptor glial cell-derived neurotrophic factor family receptor a-like (GFRAL) in gastric tumors are important for malignant progression. Moreover, GDF15 expression is increased in gastric cancer cells with cisplatin resistance, resulting from elevated intracellular glutathione (GSH) and antioxidant activities. Upregulated GDF15 could increase intracellular GSH content by activating the GFRAL-GCN2-eIF2α-ATF4 signaling, enhancing cystine-uptake transporter xCT expression, and contributing biosynthesis of GSH in human gastric cancer cells. In conclusion, our results indicate that GDF15 could induce chemoresistance by upregulating xCT expression and GSH biosynthesis in human gastric cancer cells. Targeting GDF15 could be a promising treatment method for gastric cancer progression.
MeSH term(s)	Humans ; Cisplatin/therapeutic use ; Stomach Neoplasms/drug therapy ; Stomach Neoplasms/genetics ; Stomach Neoplasms/metabolism ; Up-Regulation ; Growth Differentiation Factor 15/genetics ; Growth Differentiation Factor 15/metabolism ; Glutathione/metabolism
Chemical Substances	Cisplatin (Q20Q21Q62J) ; Growth Differentiation Factor 15 ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2023-06-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2115647-5
ISSN	1349-7006 ; 1349-7006
ISSN (online)	1349-7006
ISSN	1349-7006
DOI	10.1111/cas.15869
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Article ; Online: Mitochondrial dysfunction decreases cisplatin sensitivity in gastric cancer cells through upregulation of integrated stress response and mitokine GDF15.

Wang, Sheng-Fan / Chang, Yuh-Lih / Liu, Ting-Yu / Huang, Kuo-Hung / Fang, Wen-Liang / Li, Anna Fen-Yau / Yeh, Tien-Shun / Hung, Giun-Yi / Lee, Hsin-Chen

The FEBS journal

2023 Volume 291, Issue 6, Page(s) 1131–1150

Abstract: Gastric neoplasm is a high-mortality cancer worldwide. Chemoresistance is the obstacle against gastric cancer treatment. Mitochondrial dysfunction has been observed to promote malignant progression. However, the underlying mechanism is still unclear. The ...

Abstract	Gastric neoplasm is a high-mortality cancer worldwide. Chemoresistance is the obstacle against gastric cancer treatment. Mitochondrial dysfunction has been observed to promote malignant progression. However, the underlying mechanism is still unclear. The mitokine growth differentiation factor 15 (GDF15) is a significant biomarker for mitochondrial disorder and is activated by the integrated stress response (ISR) pathway. The serum level of GDF15 was found to be correlated with the poor prognosis of gastric cancer patients. In this study, we found that high GDF15 protein expression might increase disease recurrence in adjuvant chemotherapy-treated gastric cancer patients. Moreover, treatment with mitochondrial inhibitors, especially oligomycin (a complex V inhibitor) and salubrinal (an ISR activator), respectively, was found to upregulate GDF15 and enhance cisplatin insensitivity of human gastric cancer cells. Mechanistically, it was found that the activating transcription factor 4-C/EBP homologous protein pathway has a crucial function in the heightened manifestation of GDF15. In addition, reactive oxygen species-activated general control nonderepressible 2 mediates the oligomycin-induced ISR, and upregulates GDF15. The GDF15-glial cell-derived neurotrophic factor family receptor a-like-ISR-cystine/glutamate transporter-enhanced glutathione production was found to be involved in cisplatin resistance. These results suggest that mitochondrial dysfunction might enhance cisplatin insensitivity through GDF15 upregulation, and targeting mitokine GDF15-ISR regulation might be a strategy against cisplatin resistance of gastric cancer.
MeSH term(s)	Humans ; Cisplatin/pharmacology ; Stomach Neoplasms/pathology ; Up-Regulation ; Growth Differentiation Factor 15/genetics ; Growth Differentiation Factor 15/metabolism ; Oligomycins
Chemical Substances	Cisplatin (Q20Q21Q62J) ; Growth Differentiation Factor 15 ; Oligomycins ; GDF15 protein, human
Language	English
Publishing date	2023-11-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2173655-8
ISSN	1742-4658 ; 1742-464X
ISSN (online)	1742-4658
ISSN	1742-464X
DOI	10.1111/febs.16992
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Article ; Online: IDH-mutant myeloid neoplasms are associated with seronegative rheumatoid arthritis and innate immune activation.

Hong, Lih En / Wechalekar, Mihir D / Kutyna, Monika / Small, Annabelle / Lim, Kelly / Thompson-Peach, Chloe / Li, Joule J / Chhetri, Rakchha / Scott, Hamish S / Brown, Anna / Hahn, Christopher N / Yeung, David T / Sajid, Salvia / Robinson, Nirmal / Thomas, Ranjeny / Branford, Susan / D'Andrea, Richard J / Samaraweera, Saumya E / Patnaik, Mrinal /

Proudman, Susanna / Thomas, Daniel / Kok, Chung Hoow / Shah, Mithun V / Hiwase, Devendra K

Blood

2024 Volume 143, Issue 18, Page(s) 1873–1877

Abstract: Abstract: High prevalence of IDH mutations in seronegative rheumatoid arthritis (RA) with myeloid neoplasm, elevated 2-hydroxyglutarate, dysregulated innate immunity, and proinflammatory microenvironment suggests causative association between IDH ... ...

Abstract	Abstract: High prevalence of IDH mutations in seronegative rheumatoid arthritis (RA) with myeloid neoplasm, elevated 2-hydroxyglutarate, dysregulated innate immunity, and proinflammatory microenvironment suggests causative association between IDH mutations and seronegative RA. Our findings merit investigation of IDH inhibitors as therapeutics for seronegative IDH-mutated RA.
MeSH term(s)	Humans ; Arthritis, Rheumatoid/immunology ; Arthritis, Rheumatoid/genetics ; Immunity, Innate ; Isocitrate Dehydrogenase/genetics ; Mutation ; Male ; Female ; Middle Aged ; Aged
Chemical Substances	Isocitrate Dehydrogenase (EC 1.1.1.41) ; IDH1 protein, human (EC 1.1.1.42.)
Language	English
Publishing date	2024-04-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	80069-7
ISSN	1528-0020 ; 0006-4971
ISSN (online)	1528-0020
ISSN	0006-4971
DOI	10.1182/blood.2023023593
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Article ; Online: Adaptations of a Tertiary Otorhinolaryngology Head and Neck Surgery Department in Singapore during the COVID-19 Outbreak.

See, Anna / Go, Lih Khuang / Teo, Constance E H / Teo, Neville Wei Yang / Toh, Song Tar

The Annals of otology, rhinology, and laryngology

2020 Volume 130, Issue 2, Page(s) 177–181

Abstract: Purpose: The novel coronavirus 2019 (COVID-19) outbreak which was first reported in Wuhan, China has been declared a pandemic by the World Health Organization on March 11, 2020. Otorhinolaryngologists deal intimately with pathologies of the head and ... ...

Abstract	Purpose: The novel coronavirus 2019 (COVID-19) outbreak which was first reported in Wuhan, China has been declared a pandemic by the World Health Organization on March 11, 2020. Otorhinolaryngologists deal intimately with pathologies of the head and neck region and upper respiratory tract and have been reported as a vulnerable group of healthcare workers who may be more susceptible to COVID-19 nosocomial infection. Methods: In this article, we provide a comprehensive overview of the adaptations of Singapore's largest tertiary Otorhinolaryngology department during the COVID-19 outbreak. This was undertaken via an evidence-based approach. The relevant medical literature and evidence underlying our adaptations are highlighted. Results: A four-pronged strategy including (1) personnel segregation, (2) triaging and decantment, (3) use of personal protective equipment and (4) changes in clinical practice was employed. The strategy was bolstered by drawing upon a collective learnt experience from the 2003 Severe Acute Respiratory Syndrome (SARS) outbreak. Conclusion: A rigorous framework which can preserve operationality while navigating the heightened risks during this outbreak is critical for every Otorhinolaryngology department. As the pandemic continues to evolve and more scientific reports of this disease are made available, approaches will need to be morphed.
MeSH term(s)	COVID-19/epidemiology ; Comorbidity ; Disease Outbreaks ; Health Personnel/statistics & numerical data ; Hospital Departments/statistics & numerical data ; Humans ; Otorhinolaryngologic Diseases/epidemiology ; Otorhinolaryngologic Diseases/surgery ; Otorhinolaryngologic Surgical Procedures/methods ; SARS-CoV-2 ; Singapore/epidemiology
Keywords	covid19
Language	English
Publishing date	2020-07-29
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	120642-4
ISSN	1943-572X ; 0003-4894
ISSN (online)	1943-572X
ISSN	0003-4894
DOI	10.1177/0003489420946779
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Article: Identity of

Karami, Nahid / Kk, Sriram / Yazdanshenas, Shora / Lin, Yii-Lih / Jaén-Luchoro, Daniel / Ekedahl, Elina / Parameshwaran, Sanjana / Lindblom, Anna / Åhrén, Christina / Westerlund, Fredrik

Microorganisms

2021 Volume 9, Issue 6

Abstract: Plasmid-mediated multidrug resistance ... ...

Abstract	Plasmid-mediated multidrug resistance in
Language	English
Publishing date	2021-05-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9061138
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Article ; Online: Dextran sulfate-amplified extracellular matrix deposition promotes osteogenic differentiation of mesenchymal stem cells.

Wan, Ho-Ying / Shin, Rita Lih Ying / Chen, Jack Chun Hin / Assunção, Marisa / Wang, Dan / Nilsson, Susie K / Tuan, Rocky S / Blocki, Anna

Acta biomaterialia

2021 Volume 140, Page(s) 163–177

Abstract: The development of bone-like tissues in vitro that exhibit key features similar to those in vivo is needed to produce tissue models for drug screening and the study of bone physiology and disease pathogenesis. Extracellular matrix (ECM) is a predominant ... ...

Abstract	The development of bone-like tissues in vitro that exhibit key features similar to those in vivo is needed to produce tissue models for drug screening and the study of bone physiology and disease pathogenesis. Extracellular matrix (ECM) is a predominant component of bone in vivo; however, as ECM assembly is sub-optimal in vitro, current bone tissue engineering approaches are limited by an imbalance in ECM-to-cell ratio. We amplified the deposition of osteoblastic ECM by supplementing dextran sulfate (DxS) into osteogenically induced cultures of human mesenchymal stem cells (MSCs). DxS, previously implicated to act as a macromolecular crowder, was recently demonstrated to aggregate and co-precipitate major ECM components, including collagen type I, thereby amplifying its deposition. This effect was re-confirmed for MSC cultures undergoing osteogenic induction, where DxS supplementation augmented collagen type I deposition, accompanied by extracellular osteocalcin accumulation. The resulting differentiated osteoblasts exhibited a more mature osteogenic gene expression profile, indicated by a strong upregulation of the intermediate and late osteogenic markers ALP and OCN, respectively. The associated cellular microenvironment was also enriched in bone morphogenetic protein 2 (BMP-2). Interestingly, the resulting decellularized matrices exhibited the strongest osteo-inductive effects on re-seeded MSCs, promoted cell proliferation, osteogenic marker expression and ECM calcification. Taken together, these findings suggest that DxS-mediated enhancement of osteogenic differentiation by MSCs is mediated by the amplified ECM, which is enriched in osteo-inductive factors. We have thus established a simple and reproducible approach to generate ECM-rich bone-like tissue in vitro with sequestration of osteo-inductive factors. STATEMENT OF SIGNIFICANCE: As extracellular matrix (ECM) assembly is significantly retarded in vitro, the imbalance in ECM-to-cell ratio hampers current in vitro bone tissue engineering approaches in their ability to faithfully resemble their in vivo counterpart. We addressed this limitation by leveraging a poly-electrolyte mediated co-assembly and amplified deposition of ECM during osteogenic differentiation of human mesenchymal stem cells (MSCs). The resulting pericelluar space in culture was enriched in organic and inorganic bone ECM components, as well as osteo-inductive factors, which promoted the differentiation of MSCs towards a more mature osteoblastic phenotype. These findings thus demonstrated a simple and reproducible approach to generate ECM-rich bone-like tissue in vitro with a closer recapitulation of the in vivo tissue niche.
MeSH term(s)	Cell Differentiation ; Cells, Cultured ; Dextran Sulfate/metabolism ; Dextran Sulfate/pharmacology ; Extracellular Matrix/metabolism ; Humans ; Mesenchymal Stem Cells ; Osteogenesis
Chemical Substances	Dextran Sulfate (9042-14-2)
Language	English
Publishing date	2021-12-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2173841-5
ISSN	1878-7568 ; 1742-7061
ISSN (online)	1878-7568
ISSN	1742-7061
DOI	10.1016/j.actbio.2021.11.049
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