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  1. Article ; Online: A role for DNA methylation in bumblebee morphogenesis hints at female-specific developmental erasure.

    Hunt, Ben J / Pegoraro, Mirko / Marshall, Hollie / Mallon, Eamonn B

    Insect molecular biology

    2024  

    Abstract: Epigenetic mechanisms, such as DNA methylation, are crucial factors in animal development. In some mammals, almost all DNA methylation is erased during embryo development and re-established in a sex- and cell-specific manner. This erasure and re- ... ...

    Abstract Epigenetic mechanisms, such as DNA methylation, are crucial factors in animal development. In some mammals, almost all DNA methylation is erased during embryo development and re-established in a sex- and cell-specific manner. This erasure and re-establishment is thought to primarily be a vertebrate-specific trait. Insects are particularly interesting in terms of development as many species often undergo remarkable morphological changes en route to maturity, that is, morphogenesis. However, little is known about the role of epigenetic mechanisms in this process across species. We have used whole-genome bisulfite sequencing to track genome-wide DNA methylation changes through the development of an economically and environmentally important pollinator species, the bumblebee Bombus terrestris (Hymenoptera:Apidae Linnaeus). We find overall levels of DNA methylation vary throughout development, and we find developmentally relevant differentially methylated genes throughout. Intriguingly, we have identified a depletion of DNA methylation in ovaries/eggs and an enrichment of highly methylated genes in sperm. We suggest this could represent a sex-specific DNA methylation erasure event. To our knowledge, this is the first suggestion of possible developmental DNA methylation erasure in an insect species. This study lays the required groundwork for functional experimental work to determine if there is a causal nature to the DNA methylation differences identified. Additionally, the application of single-cell methylation sequencing to this system will enable more accurate identification of if or when DNA methylation is erased during development.
    Language English
    Publishing date 2024-02-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 33602-6
    ISSN 1365-2583 ; 0962-1075
    ISSN (online) 1365-2583
    ISSN 0962-1075
    DOI 10.1111/imb.12897
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    In stock of ZB MED Cologne/Königswinter

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    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  2. Article ; Online: Malaria in the 'Omics Era'.

    Pegoraro, Mirko / Weedall, Gareth D

    Genes

    2021  Volume 12, Issue 6

    Abstract: Genomics has revolutionised the study of the biology of parasitic diseases. The first Eukaryotic parasite to have its genome sequenced was the malaria ... ...

    Abstract Genomics has revolutionised the study of the biology of parasitic diseases. The first Eukaryotic parasite to have its genome sequenced was the malaria parasite
    MeSH term(s) Epigenome ; Genome, Protozoan ; Humans ; Malaria/parasitology ; Plasmodium falciparum/genetics ; Plasmodium falciparum/metabolism ; Polymorphism, Genetic
    Language English
    Publishing date 2021-05-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes12060843
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  3. Article: Malaria in the ‘Omics Era’

    Pegoraro, Mirko / Weedall, Gareth D.

    Genes. 2021 May 30, v. 12, no. 6

    2021  

    Abstract: Genomics has revolutionised the study of the biology of parasitic diseases. The first Eukaryotic parasite to have its genome sequenced was the malaria parasite Plasmodium falciparum. Since then, Plasmodium genomics has continued to lead the way in the ... ...

    Abstract Genomics has revolutionised the study of the biology of parasitic diseases. The first Eukaryotic parasite to have its genome sequenced was the malaria parasite Plasmodium falciparum. Since then, Plasmodium genomics has continued to lead the way in the study of the genome biology of parasites, both in breadth—the number of Plasmodium species’ genomes sequenced—and in depth—massive-scale genome re-sequencing of several key species. Here, we review some of the insights into the biology, evolution and population genetics of Plasmodium gained from genome sequencing, and look at potential new avenues in the future genome-scale study of its biology.
    Keywords Plasmodium falciparum ; evolution ; genomics ; malaria ; parasites ; population genetics
    Language English
    Dates of publication 2021-0530
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes12060843
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  4. Article: Nucleotide Variation in

    Pegoraro, Mirko / Sayegh Rezek, Emily / Fishman, Bettina / Tauber, Eran

    Frontiers in physiology

    2022  Volume 13, Page(s) 781380

    Abstract: Cryptochrome (CRY) is a conserved protein associated with the circadian clock in a broad range of organisms, including plants, insects, and mammals. ... ...

    Abstract Cryptochrome (CRY) is a conserved protein associated with the circadian clock in a broad range of organisms, including plants, insects, and mammals. In
    Language English
    Publishing date 2022-02-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2022.781380
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  5. Article ; Online: Adaptation of

    Deppisch, Peter / Prutscher, Johanna M / Pegoraro, Mirko / Tauber, Eran / Wegener, Christian / Helfrich-Förster, Charlotte

    Journal of biological rhythms

    2022  Volume 37, Issue 2, Page(s) 185–201

    Abstract: Circadian clocks help animals to be active at the optimal time of the day whereby for most species the daily light-dark cycle is the most important zeitgeber for their circadian clock. In this respect, long arctic summer days are particularly challenging ...

    Abstract Circadian clocks help animals to be active at the optimal time of the day whereby for most species the daily light-dark cycle is the most important zeitgeber for their circadian clock. In this respect, long arctic summer days are particularly challenging as light is present almost 24 h per day, and continuous light makes the circadian clocks of many animals arrhythmic. This is especially true for the fruit fly,
    MeSH term(s) Alleles ; Animals ; Circadian Rhythm/genetics ; Cryptochromes ; Drosophila/physiology ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/physiology ; Light ; Photoperiod
    Chemical Substances Cryptochromes ; Drosophila Proteins
    Language English
    Publishing date 2022-03-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 896387-3
    ISSN 1552-4531 ; 0748-7304
    ISSN (online) 1552-4531
    ISSN 0748-7304
    DOI 10.1177/07487304221082448
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2872: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Photoperiod-Dependent Expression of MicroRNA in

    Pegoraro, Mirko / Fishman, Bettina / Zonato, Valeria / Zouganelis, Georgios / Francis, Amanda / Kyriacou, Charalambos P / Tauber, Eran

    International journal of molecular sciences

    2022  Volume 23, Issue 9

    Abstract: Like many other insects in temperate regions, ...

    Abstract Like many other insects in temperate regions,
    MeSH term(s) Animals ; Diapause ; Drosophila/genetics ; Drosophila melanogaster/genetics ; MicroRNAs/genetics ; Photoperiod
    Chemical Substances MIRN34 microRNA, Drosophila ; MicroRNAs ; Mirn184 microRNA, Drosophila
    Language English
    Publishing date 2022-04-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23094935
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  7. Article ; Online: Photoperiod-Dependent Expression of MicroRNA in Drosophila

    Mirko Pegoraro / Bettina Fishman / Valeria Zonato / Georgios Zouganelis / Amanda Francis / Charalambos P. Kyriacou / Eran Tauber

    International Journal of Molecular Sciences, Vol 23, Iss 4935, p

    2022  Volume 4935

    Abstract: Like many other insects in temperate regions, Drosophila melanogaster exploits the photoperiod shortening that occurs during the autumn as an important cue to trigger a seasonal response. Flies survive the winter by entering a state of reproductive ... ...

    Abstract Like many other insects in temperate regions, Drosophila melanogaster exploits the photoperiod shortening that occurs during the autumn as an important cue to trigger a seasonal response. Flies survive the winter by entering a state of reproductive arrest (diapause), which drives the relocation of resources from reproduction to survival. Here, we profiled the expression of microRNA (miRNA) in long and short photoperiods and identified seven differentially expressed miRNAs ( dme-mir-2b , dme-mir-11 , dme-mir-34 , dme-mir-274 , dme-mir-184 , dme-mir-184* , and dme-mir-285 ). Misexpression of dme-mir-2b , dme-mir-184 , and dme-mir-274 in pigment-dispersing, factor-expressing neurons largely disrupted the normal photoperiodic response, suggesting that these miRNAs play functional roles in photoperiodic timing. We also analyzed the targets of photoperiodic miRNA by both computational predication and by Argonaute-1-mediated immunoprecipitation of long- and short-day RNA samples. Together with global transcriptome profiling, our results expand existing data on other Drosophila species, identifying genes and pathways that are differentially regulated in different photoperiods and reproductive status. Our data suggest that post-transcriptional regulation by miRNA is an important facet of photoperiodic timing.
    Keywords diapause ; Drosophila ; microRNA ; photoperiodism ; RNA immunoprecipitation ; seasonal timing ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 590
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Do social insects support Haig's kin theory for the evolution of genomic imprinting?

    Pegoraro, Mirko / Marshall, Hollie / Lonsdale, Zoë N / Mallon, Eamonn B

    Epigenetics

    2017  Volume 12, Issue 9, Page(s) 725–742

    Abstract: Although numerous imprinted genes have been described in several lineages, the phenomenon of genomic imprinting presents a peculiar evolutionary problem. Several hypotheses have been proposed to explain gene imprinting, the most supported being Haig's ... ...

    Abstract Although numerous imprinted genes have been described in several lineages, the phenomenon of genomic imprinting presents a peculiar evolutionary problem. Several hypotheses have been proposed to explain gene imprinting, the most supported being Haig's kinship theory. This theory explains the observed pattern of imprinting and the resulting phenotypes as a competition for resources between related individuals, but despite its relevance it has not been independently tested. Haig's theory predicts that gene imprinting should be present in eusocial insects in many social scenarios. These lineages are therefore ideal for testing both the theory's predictions and the mechanism of gene imprinting. Here we review the behavioral evidence of genomic imprinting in eusocial insects, the evidence of a mechanism for genomic imprinting and finally we evaluate recent results showing parent of origin allele specific expression in honeybees in the light of Haig's theory.
    MeSH term(s) Animals ; Bees/genetics ; Chromatin Assembly and Disassembly ; DNA Methylation ; Epigenomics ; Evolution, Molecular ; Genomic Imprinting ; Models, Genetic ; Social Behavior
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1559-2308
    ISSN (online) 1559-2308
    DOI 10.1080/15592294.2017.1348445
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  9. Article ; Online: The genetic basis of diurnal preference in Drosophila melanogaster.

    Pegoraro, Mirko / Flavell, Laura M M / Menegazzi, Pamela / Colombi, Perrine / Dao, Pauline / Helfrich-Förster, Charlotte / Tauber, Eran

    BMC genomics

    2020  Volume 21, Issue 1, Page(s) 596

    Abstract: Background: Most animals restrict their activity to a specific part of the day, being diurnal, nocturnal or crepuscular. The genetic basis underlying diurnal preference is largely unknown. Under laboratory conditions, Drosophila melanogaster is ... ...

    Abstract Background: Most animals restrict their activity to a specific part of the day, being diurnal, nocturnal or crepuscular. The genetic basis underlying diurnal preference is largely unknown. Under laboratory conditions, Drosophila melanogaster is crepuscular, showing a bi-modal activity profile. However, a survey of strains derived from wild populations indicated that high variability among individuals exists, including flies that are nocturnal.
    Results: Using a highly diverse population, we performed an artificial selection experiment, selecting flies with extreme diurnal or nocturnal preference. After 10 generations, we obtained highly diurnal and nocturnal strains. We used whole-genome expression analysis to identify differentially expressed genes in diurnal, nocturnal and crepuscular (control) flies. Other than one circadian clock gene (pdp1), most differentially expressed genes were associated with either clock output (pdf, to) or input (Rh3, Rh2, msn). This finding was congruent with behavioural experiments indicating that both light masking and the circadian pacemaker are involved in driving nocturnality.
    Conclusions: Our study demonstrates that genetic variation segregating in wild populations contributes to substantial variation in diurnal preference. We identified candidate genes associated with diurnality/nocturnality, while data emerging from our expression analysis and behavioural experiments suggest that both clock and clock-independent pathways are involved in shaping diurnal preference. The diurnal and nocturnal selection strains provide us with a unique opportunity to understand the genetic architecture of diurnal preference.
    MeSH term(s) Animals ; Circadian Clocks/genetics ; Circadian Rhythm/genetics ; Drosophila melanogaster/genetics ; Motor Activity
    Language English
    Publishing date 2020-08-31
    Publishing country England
    Document type Journal Article
    ISSN 1471-2164
    ISSN (online) 1471-2164
    DOI 10.1186/s12864-020-07020-z
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  10. Article ; Online: Interspecific studies of circadian genes period and timeless in Drosophila.

    Noreen, Shumaila / Pegoraro, Mirko / Nouroz, Faisal / Tauber, Eran / Kyriacou, Charalambos P

    Gene

    2018  Volume 648, Page(s) 106–114

    Abstract: The level of rescue of clock function in genetically arrhythmic Drosophila melanogaster hosts using interspecific clock gene transformation was used to study the putative intermolecular coevolution between interacting clock proteins. Among them PER and ... ...

    Abstract The level of rescue of clock function in genetically arrhythmic Drosophila melanogaster hosts using interspecific clock gene transformation was used to study the putative intermolecular coevolution between interacting clock proteins. Among them PER and TIM are the two important negative regulators of the circadian clock feedback loop. We transformed either the D. pseudoobscura per or tim transgenes into the corresponding arrhythmic D. melanogaster mutant (per01 or tim01) and observed >50% rhythmicity but the period of activity rhythm was either longer (D. pseudoobscura-per) or shorter than 24 h (D. pseudoobscura-tim) compared to controls. By introducing both transgenes simultaneously into double mutants, we observed that the period of the activity rhythm was rescued by the pair of hemizygous transgenes (~24 h). These flies also showed a more optimal level of temperature compensation for the period. Under LD 12:12 these flies have a D. pseudoobscura like activity profile with the absence of morning anticipation as well as a very prominent earlier evening peak of activity rhythm. These observation are consistent with the view that TIM and PER form a heterospecific coevolved module at least for the circadian period of activity rhythms. However the strength of rhythmicity was reduced by having both transgenes present, so while evidence for a coevolution between PER and TIM is observed for some characters it is not for others.
    MeSH term(s) Animals ; Animals, Genetically Modified ; Circadian Rhythm/genetics ; Drosophila/classification ; Drosophila/genetics ; Drosophila/metabolism ; Drosophila Proteins/classification ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Genetic Complementation Test ; Motor Activity/genetics ; Mutation ; Period Circadian Proteins/classification ; Period Circadian Proteins/genetics ; Period Circadian Proteins/metabolism ; Phylogeny ; Species Specificity ; Temperature ; Time Factors
    Chemical Substances Drosophila Proteins ; PER protein, Drosophila ; Period Circadian Proteins ; tim protein, Drosophila
    Language English
    Publishing date 2018-02-04
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/j.gene.2018.01.020
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1357: Show issues Location:
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