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Article ; Online: Evaluation of Biofilm Formation, Hydrolytic Eenzymes and Antifungal Susceptibility of Planktonic Cells of Candida Albicans Species Isolated from Different Clinical Samples

Faezeh Mohammadi / Nima Hemmat

مجله دانشکده پزشکی اصفهان, Vol 40, Iss 696, Pp 942-

2023 Volume 949

Abstract: Background: Candida species are common organisms in human and animal mucosa that cause a wide range of Candida infections in immunocompromised patients. This study investigated the ability to produce proteinase, phospholipase and hemolysin as well as ... ...

Abstract	Background: Candida species are common organisms in human and animal mucosa that cause a wide range of Candida infections in immunocompromised patients. This study investigated the ability to produce proteinase, phospholipase and hemolysin as well as biofilm formation in different clinical isolates of Candida albicans.Methods: In this study, ninety-four C. albicans were identified using phenotypic tests and amplification of the hyphal wall protein (HWP1) gene, and the proteinase, phospholipase and hemolysin production in specific mediums, as well as the ability to biofilm formation using the crystal violet method were evaluated. Then, the antifungal susceptibility of planktonic cells was tested on the basis of the CLSI- M27-A3/S protocol.Findings: In this study, the proteinase, phospholipase and hemolysin activities of C.albicans isolated from different body sites were 82%, 75.5%, and 68%, respectively. Additionally, 74.5% of the isolates had the ability to biofilm formation. Among the isolates being studied, the strains isolated from the oral cavity showed the highest activity of proteinase, hemolysin and biofilm formation, and the strains isolated from vaginal secretions showed the highest level of phospholipase activity. The susceptibility pattern of C. albicans species to antifungals showed that all isolates were sensitive to AMB and VRC, and resistance to FLC and ITC was reported as 5.4% and 2.2%, respectively.Conclusion: The results show the importance of molecular epidemiology studies and understanding the role of hydrolytic enzymes and biofilm production in C. albicans strains.
Keywords	candida albicans ; antifungal agents ; disease susceptibility ; virulence ; biofilms ; Medicine ; R ; Medicine (General) ; R5-920
Subject code	610
Language	Persian
Publishing date	2023-01-01T00:00:00Z
Publisher	Vesnu Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: HSV1 microRNAs in glioblastoma development: an in silico study.

Abdoli Shadbad, Mahdi / Hemmat, Nima / Abdoli Shadbad, Mahla / Brunetti, Oronzo / Silvestris, Nicola / Baradaran, Behzad

Scientific reports

2024 Volume 14, Issue 1, Page(s) 27

Abstract: Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor. Recent findings highlighted the significance of viral microRNAs (miRs) in regulating post-transcriptional mRNA expression in various human conditions. Although HSV1 encodes viral ... ...

Abstract	Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor. Recent findings highlighted the significance of viral microRNAs (miRs) in regulating post-transcriptional mRNA expression in various human conditions. Although HSV1 encodes viral miRs and affects the central nervous system, no study investigated the roles of HSV1-encoding miRs in GBM development. This study applied in silico approaches to investigate whether HSV1-encoding miRs are involved in GBM development and, if so, how they regulate tumor-suppressive/oncogenes expression in GBM. This study leveraged bioinformatics approaches to identify the potential effect of HSV1 miRs in GBM development. The GSE158284, GSE153679, and GSE182109 datasets were analyzed to identify differentially expressed genes in GBM tissues and cell lines using the limma package in the R software. The GSE182109 dataset was analyzed to determine gene expression at the single-cell levels using the Seurat package in the R software. The TCGA-GTEX, GDSC, CTRP, immunogenetic, and enrichment analyses were performed to study the impact of identified viral HSV1 miRs targets in GBM development. hsv1-miR-H6-3p is upregulated in GBM and can be responsible for EPB41L1 and SH3PXD2A downregulation in GBM tissues. Also, hsv1-miR-H1-5p is upregulated in GBM and can decrease the expression of MELK, FZD2, NOVA1, TMEM97, PTPRZ1, and PDGFC in GBM development. The single-cell RNA sequencing analyses have demonstrated that MELK, FZD2, NOVA1, TMEM97, PTPRZ1, and PDGFC are expressed in astrocytes residing in the GBM microenvironment. This study provides novel insights into the potential roles of HSV1 miRs in GBM pathogenesis and offers a reference for further studies on the significance of HSV1 miRs in GBM development.
MeSH term(s)	Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Glioblastoma/pathology ; Brain Neoplasms/pathology ; Cell Line ; Herpesvirus 1, Human/genetics ; Herpesvirus 1, Human/metabolism ; RNA-Binding Proteins/metabolism ; Gene Expression Regulation, Neoplastic ; Cell Line, Tumor ; Cell Proliferation ; Tumor Microenvironment ; Protein Serine-Threonine Kinases/metabolism ; Receptor-Like Protein Tyrosine Phosphatases, Class 5/metabolism
Chemical Substances	MicroRNAs ; RNA-Binding Proteins ; MELK protein, human (EC 2.7.1.-) ; Protein Serine-Threonine Kinases (EC 2.7.11.1) ; PTPRZ1 protein, human (EC 3.1.3.48) ; Receptor-Like Protein Tyrosine Phosphatases, Class 5 (EC 3.1.3.48)
Language	English
Publishing date	2024-01-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-45249-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: siRNA-E6 sensitizes HPV-16-related cervical cancer through Oxaliplatin: an in vitro study on anti-cancer combination therapy.

Shiri Aghbash, Parisa / Hemmat, Nima / Baradaran, Behzad / Bannazadeh Baghi, Hossein

European journal of medical research

2023 Volume 28, Issue 1, Page(s) 42

Abstract: Background: Persistent infection with high-risk Human papillomaviruses (HPV), such as hr-HPV-16 and hr-HPV-18, lead to cervical cancer, the fourth most common cancer in the world. In the present study, we investigated the alteration of E6 oncogene ... ...

Abstract	Background: Persistent infection with high-risk Human papillomaviruses (HPV), such as hr-HPV-16 and hr-HPV-18, lead to cervical cancer, the fourth most common cancer in the world. In the present study, we investigated the alteration of E6 oncogene expression by E6-specific short interfering RNA (siRNA) combined with Oxaliplatin. Methods: The cervical cancer cell line, CaSki, was transfected with E6-siRNA, then treated with Oxaliplatin. The cellular genes, such as p53, MMP9, Nanog, and caspases expression, were assessed by quantitative real-time PCR. The cell death rate, cell cycle, and cell viability were assessed by Annexin V/PI staining, DAPI staining, and MTT test, respectively. Furthermore, colony formation assay and scratch test determined the stemness ability and cell metastasis, respectively. Results: Combination therapy increased the re-expression of genes involved in the p53-dependent apoptosis pathway (increase in apoptosis to 44.2%), and reduced stemness and metastasis ability compared to either siRNA or Oxaliplatin monotherapy. Together, our results demonstrate that E6-siRNA and Oxaliplatin combination increased the cervical cancer cells' sensitivity to Oxaliplatin and decreased the survival rate, proliferation, and metastasis, and consequently escalated apoptosis rate, induced cell cycle arrest in the sub-G1 stage, and reduced the chemotherapy drug dosage. Conclusion: Inhibition of E6 oncogene expression and subsequent E6-siRNA with Oxaliplatin combination therapy could be a novel strategy for cervical cancer treatment.
MeSH term(s)	Female ; Humans ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; RNA, Small Interfering/pharmacology ; Oxaliplatin/pharmacology ; Oxaliplatin/therapeutic use ; Oxaliplatin/metabolism ; Uterine Cervical Neoplasms/drug therapy ; Uterine Cervical Neoplasms/genetics ; Human papillomavirus 16/genetics ; Human papillomavirus 16/metabolism ; Tumor Suppressor Protein p53/genetics ; Oncogene Proteins, Viral/genetics ; Oncogene Proteins, Viral/metabolism ; Apoptosis/genetics
Chemical Substances	RNA, Small Interfering ; Oxaliplatin (04ZR38536J) ; Tumor Suppressor Protein p53 ; Oncogene Proteins, Viral
Language	English
Publishing date	2023-01-21
Publishing country	England
Document type	Journal Article
ZDB-ID	1329381-3
ISSN	2047-783X ; 0949-2321
ISSN (online)	2047-783X
ISSN	0949-2321
DOI	10.1186/s40001-023-01014-9
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Article ; Online: HSV1 microRNAs in glioblastoma development

Mahdi Abdoli Shadbad / Nima Hemmat / Mahla Abdoli Shadbad / Oronzo Brunetti / Nicola Silvestris / Behzad Baradaran

Scientific Reports, Vol 14, Iss 1, Pp 1-

an in silico study

2024 Volume 12

Abstract: Abstract Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor. Recent findings highlighted the significance of viral microRNAs (miRs) in regulating post-transcriptional mRNA expression in various human conditions. Although HSV1 ... ...

Abstract	Abstract Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor. Recent findings highlighted the significance of viral microRNAs (miRs) in regulating post-transcriptional mRNA expression in various human conditions. Although HSV1 encodes viral miRs and affects the central nervous system, no study investigated the roles of HSV1-encoding miRs in GBM development. This study applied in silico approaches to investigate whether HSV1-encoding miRs are involved in GBM development and, if so, how they regulate tumor-suppressive/oncogenes expression in GBM. This study leveraged bioinformatics approaches to identify the potential effect of HSV1 miRs in GBM development. The GSE158284, GSE153679, and GSE182109 datasets were analyzed to identify differentially expressed genes in GBM tissues and cell lines using the limma package in the R software. The GSE182109 dataset was analyzed to determine gene expression at the single-cell levels using the Seurat package in the R software. The TCGA-GTEX, GDSC, CTRP, immunogenetic, and enrichment analyses were performed to study the impact of identified viral HSV1 miRs targets in GBM development. hsv1-miR-H6-3p is upregulated in GBM and can be responsible for EPB41L1 and SH3PXD2A downregulation in GBM tissues. Also, hsv1-miR-H1-5p is upregulated in GBM and can decrease the expression of MELK, FZD2, NOVA1, TMEM97, PTPRZ1, and PDGFC in GBM development. The single-cell RNA sequencing analyses have demonstrated that MELK, FZD2, NOVA1, TMEM97, PTPRZ1, and PDGFC are expressed in astrocytes residing in the GBM microenvironment. This study provides novel insights into the potential roles of HSV1 miRs in GBM pathogenesis and offers a reference for further studies on the significance of HSV1 miRs in GBM development.
Keywords	Medicine ; R ; Science ; Q
Subject code	570
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
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Article ; Online: Covid-19: Perspectives on Innate Immune Evasion.

Taefehshokr, Nima / Taefehshokr, Sina / Hemmat, Nima / Heit, Bryan

Frontiers in immunology

2020 Volume 11, Page(s) 580641

Abstract: The ongoing outbreak of Coronavirus disease 2019 infection achieved pandemic status on March 11, 2020. As of September 8, 2020 it has caused over 890,000 mortalities world-wide. Coronaviral infections are enabled by potent immunoevasory mechanisms that ... ...

Abstract	The ongoing outbreak of Coronavirus disease 2019 infection achieved pandemic status on March 11, 2020. As of September 8, 2020 it has caused over 890,000 mortalities world-wide. Coronaviral infections are enabled by potent immunoevasory mechanisms that target multiple aspects of innate immunity, with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) able to induce a cytokine storm, impair interferon responses, and suppress antigen presentation on both MHC class I and class II. Understanding the immune responses to SARS-CoV-2 and its immunoevasion approaches will improve our understanding of pathogenesis, virus clearance, and contribute toward vaccine and immunotherepeutic design and evaluation. This review discusses the known host innate immune response and immune evasion mechanisms driving SARS-CoV-2 infection and pathophysiology.
MeSH term(s)	Betacoronavirus/immunology ; COVID-19 ; Coronavirus Infections/immunology ; Coronavirus Infections/pathology ; Cytokine Release Syndrome/immunology ; Cytokine Release Syndrome/pathology ; Cytokines/blood ; Humans ; Immune Evasion/immunology ; Immunity, Innate/immunology ; Pandemics ; Pneumonia, Viral/immunology ; Pneumonia, Viral/pathology ; SARS-CoV-2 ; Severe Acute Respiratory Syndrome/immunology ; Severe Acute Respiratory Syndrome/pathology
Chemical Substances	Cytokines
Keywords	covid19
Language	English
Publishing date	2020-09-30
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2020.580641
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Effects of neglect and stigmatisation on post-therapy behaviour of patients who are HIV-positive.

Hemmat, Nima / Bannazadeh Baghi, Hossein

Sexually transmitted infections

2019 Volume 95, Issue 7, Page(s) 548

MeSH term(s)	Anti-HIV Agents/therapeutic use ; Drug Utilization/statistics & numerical data ; HIV Infections/drug therapy ; HIV Infections/epidemiology ; HIV Infections/psychology ; Humans ; Middle East ; Social Isolation ; Stereotyping ; Substance Abuse, Intravenous/complications ; Substance Abuse, Intravenous/psychology
Chemical Substances	Anti-HIV Agents
Language	English
Publishing date	2019-10-18
Publishing country	England
Document type	Letter
ZDB-ID	1420303-0
ISSN	1472-3263 ; 1368-4973
ISSN (online)	1472-3263
ISSN	1368-4973
DOI	10.1136/sextrans-2019-054031
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Article ; Online: Covid-19

Nima Taefehshokr / Sina Taefehshokr / Nima Hemmat / Bryan Heit

Frontiers in Immunology, Vol

Perspectives on Innate Immune Evasion

2020 Volume 11

Abstract	The ongoing outbreak of Coronavirus disease 2019 infection achieved pandemic status on March 11, 2020. As of September 8, 2020 it has caused over 890,000 mortalities world-wide. Coronaviral infections are enabled by potent immunoevasory mechanisms that target multiple aspects of innate immunity, with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) able to induce a cytokine storm, impair interferon responses, and suppress antigen presentation on both MHC class I and class II. Understanding the immune responses to SARS-CoV-2 and its immunoevasion approaches will improve our understanding of pathogenesis, virus clearance, and contribute toward vaccine and immunotherepeutic design and evaluation. This review discusses the known host innate immune response and immune evasion mechanisms driving SARS-CoV-2 infection and pathophysiology.
Keywords	SARS-CoV-2 ; macropahge ; innnate immune ; MHC trafficking ; cytokine - immunological terms ; Immunologic diseases. Allergy ; RC581-607 ; covid19
Subject code	570
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Analysis of Biofilm-Related Genes and Antifungal Susceptibility Pattern of Vaginal

Mohammadi, Faezeh / Hemmat, Nima / Bajalan, Zahra / Javadi, Amir

BioMed research international

2021 Volume 2021, Page(s) 5598907

Abstract: Background: Vulvovaginal candidiasis caused by : Methods: We investigated the molecular identification of 70 vaginal isolates of : Results: Our findings showed that the most common yeast isolated from vaginal discharge ... ...

Abstract	Background: Vulvovaginal candidiasis caused by Methods: We investigated the molecular identification of 70 vaginal isolates of Results: Our findings showed that the most common yeast isolated from vaginal discharge was
MeSH term(s)	Adult ; Amphotericin B/pharmacology ; Antifungal Agents/chemistry ; Biofilms ; Biomass ; Candida albicans/pathogenicity ; Candida glabrata/drug effects ; Candidiasis, Vulvovaginal/microbiology ; Drug Resistance, Fungal/drug effects ; Female ; Fluconazole/pharmacology ; Humans ; Itraconazole/pharmacology ; Microbial Sensitivity Tests ; Microscopy, Electron, Scanning ; Middle Aged ; Polymorphism, Restriction Fragment Length ; Vagina/microbiology ; Virulence ; Voriconazole/pharmacology ; Young Adult
Chemical Substances	Antifungal Agents ; Itraconazole (304NUG5GF4) ; Amphotericin B (7XU7A7DROE) ; Fluconazole (8VZV102JFY) ; Voriconazole (JFU09I87TR)
Language	English
Publishing date	2021-05-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2021/5598907
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Article ; Online: Covid-19

Taefehshokr, Nima / Taefehshokr, Sina / Hemmat, Nima / Heit, Bryan

Frontiers in Immunology

Perspectives on Innate Immune Evasion

2020 Volume 11

Keywords	covid19
Publisher	Frontiers Media SA
Publishing country	ch
Document type	Article ; Online
ZDB-ID	2606827-8
ISSN	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2020.580641
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Monoclonal antibodies in cervical malignancy-related HPV.

Aghbash, Parisa Shiri / Hemmat, Nima / Fathi, Hamidreza / Baghi, Hossein Bannazadeh

Frontiers in oncology

2022 Volume 12, Page(s) 904790

Abstract: Despite many efforts to treat HPV infection, cervical cancer survival is still poor for several reasons, including resistance to chemotherapy and relapse. Numerous treatments such as surgery, radiation therapy, immune cell-based therapies, siRNA combined ...

Abstract	Despite many efforts to treat HPV infection, cervical cancer survival is still poor for several reasons, including resistance to chemotherapy and relapse. Numerous treatments such as surgery, radiation therapy, immune cell-based therapies, siRNA combined with various drugs, and immunotherapy are being studied and performed to provide the best treatment. Depending on the stage and size of the tumor, methods such as radical hysterectomy, pelvic lymphadenectomy, or chemotherapy can be utilized to treat cervical cancer. While accepted, these treatments lead to interruptions in cellular pathways and immune system homeostasis. In addition to a low survival rate, cervical neoplasm incidence has been rising significantly. However, new strategies have been proposed to increase patient survival while reducing the toxicity of chemotherapy, including targeted therapy and monoclonal antibodies. In this article, we discuss the types and potential therapeutic roles of monoclonal antibodies in cervical cancer.
Language	English
Publishing date	2022-10-06
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2022.904790
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