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Article ; Online: Sensitivity and specificity of the ICDAS II system and bitewing radiographs for detecting occlusal caries using the Spectra™ caries detection system as the reference test in children.

Caceda, Jorge H / Jiang, Shuying / Calderon, Victor / Villavicencio-Caparo, Ebingen

2023 Volume 23, Issue 1, Page(s) 896

Abstract: Background: Most studies assessing the sensitivity and specificity of caries detection methods have been performed in vitro using the histological method as the gold standard showing inconsistent values. The aim of this study was to compare the ... ...

Abstract	Background: Most studies assessing the sensitivity and specificity of caries detection methods have been performed in vitro using the histological method as the gold standard showing inconsistent values. The aim of this study was to compare the sensitivity and specificity in detecting occlusal caries using the International Caries Detection and Assessment System (ICDAS II) with the radiographic method (RM), while using the Spectra™ Caries Detection System (SCDS) as the reference test. Methods: One hundred sixty children, ages 7-12 years, participated in the study. Five zones in the occlusal surfaces of 859 primary and 632 first permanent molars were examined visually using ICDAS-II, the RM using bitewing radiographs and SCDS. The descriptive statistics of sensitivity and specificity were calculated and compared. Results: For all molars combined and for primary molars only, the sensitivity of ICDAS II was higher for detecting total caries (p < 0.001), caries in enamel (p < 0.001), and caries in dentin (p = 0.016), but it was not different for detecting caries in the dentin of permanent first molars (p = 0.214), and primary second molars (p = 0.761). The specificity of RM was higher for detecting total caries, caries in enamel for all molars combined and for permanent first molars (p < 0.001). For caries in dentin, the specificity of ICDAS II was higher for all molars combined and for primary molars only (p < 0.001). For total caries in primary molars only, and caries in dentin of permanent first molars only, the specificity was not different (p = 0.156 and p = 0.181 respectively). Conclusions: The sensitivity and specificity of ICDAS II and RM changes depending on whether the carious lesion compromises the enamel or dentin, and if the caries detection is performed in the primary molars or permanent first molars.
MeSH term(s)	Child ; Humans ; Dental Caries Susceptibility ; Sensitivity and Specificity ; Dental Caries/diagnostic imaging ; Dental Caries/pathology ; Dental Enamel/pathology ; Molar/diagnostic imaging ; Molar/pathology ; Reproducibility of Results ; Dentin/diagnostic imaging
Language	English
Publishing date	2023-11-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2091511-1
ISSN	1472-6831 ; 1472-6831
ISSN (online)	1472-6831
ISSN	1472-6831
DOI	10.1186/s12903-023-03615-6
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Article ; Online: Effect of exposure to low doses of ozone on interleukin 17A expression during progressive neurodegeneration in the rat hippocampus.

Solleiro-Villavicencio, H / Hernández-Orozco, E / Rivas-Arancibia, S

Neurologia (Barcelona, Spain)

2020 Volume 36, Issue 9, Page(s) 673–680

Abstract: Introduction: Chronic exposure to low doses of ozone causes oxidative stress and loss of regulation of the inflammatory response, leading to progressive neurodegeneration.: Objective: We studied the effect of chronic exposure to low doses of ozone on ...

Abstract	Introduction: Chronic exposure to low doses of ozone causes oxidative stress and loss of regulation of the inflammatory response, leading to progressive neurodegeneration. Objective: We studied the effect of chronic exposure to low doses of ozone on IL-17A concentration and expression in neurons, microglia, astrocytes, and T cells in the rat hippocampus. Methods: We used 72 Wistar rats, divided into 6 groups (n=12): a control group (no ozone exposure) and 5 groups exposed to ozone (0.25ppm, 4h daily) for 7, 15, 30, 60, and 90 days. We processed 6 rats from each group to quantify IL-17A by ELISA; the remaining 6 were processed for immunohistochemistry (against IL-17A and GFAP, Iba1, NeuN, and CD3). Results: The ELISA study data showed a significant increase in IL-17A concentrations in the 7-, 15-, 30-, and 60-day exposure groups, with regard to the control group (P<.05). Furthermore, they indicate that hippocampal neurons were the cells showing greatest immunoreactivity against IL-17A between 60 and 90 days of exposure to ozone; we also observed an increase in activated astrocytes in the 30- and 60-day exposure groups. Conclusion: Exposure to ozone in rats induces an increase in IL-17A expression, mainly in hippocampal neurons, accompanied by hippocampal astrocyte activation during chronic neurodegeneration, similar to that observed in Alzheimer disease in humans.
MeSH term(s)	Animals ; Hippocampus/metabolism ; Interleukin-17/metabolism ; Microglia/metabolism ; Ozone/adverse effects ; Rats ; Rats, Wistar
Chemical Substances	Interleukin-17 ; Ozone (66H7ZZK23N)
Language	English
Publishing date	2020-09-22
Publishing country	Spain
Document type	Journal Article
ZDB-ID	2654369-2
ISSN	2173-5808 ; 2173-5808
ISSN (online)	2173-5808
ISSN	2173-5808
DOI	10.1016/j.nrleng.2018.08.003
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Article: A FLEXIBLE BAYESIAN FRAMEWORK TO ESTIMATE AGE- AND CAUSE-SPECIFIC CHILD MORTALITY OVER TIME FROM SAMPLE REGISTRATION DATA.

Schumacher, Austin E / McCormick, Tyler H / Wakefield, Jon / Chu, Yue / Perin, Jamie / Villavicencio, Francisco / Simon, Noah / Liu, Li

The annals of applied statistics

2022 Volume 16, Issue 1, Page(s) 124–143

Abstract: In order to implement disease-specific interventions in young age groups, policy makers in low- and middle-income countries require timely and accurate estimates of age- and cause-specific child mortality. High-quality data is not available in settings ... ...

Abstract	In order to implement disease-specific interventions in young age groups, policy makers in low- and middle-income countries require timely and accurate estimates of age- and cause-specific child mortality. High-quality data is not available in settings where these interventions are most needed, but there is a push to create sample registration systems that collect detailed mortality information. current methods that estimate mortality from this data employ multistage frameworks without rigorous statistical justification that separately estimate all-cause and cause-specific mortality and are not sufficiently adaptable to capture important features of the data. We propose a flexible Bayesian modeling framework to estimate age- and cause-specific child mortality from sample registration data. We provide a theoretical justification for the framework, explore its properties via simulation, and use it to estimate mortality trends using data from the Maternal and Child Health Surveillance System in China.
Language	English
Publishing date	2022-03-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2376910-5
ISSN	1941-7330 ; 1932-6157
ISSN (online)	1941-7330
ISSN	1932-6157
DOI	10.1214/21-aoas1489
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Article ; Online: National population-based estimates for major birth defects, 2016-2020.

Stallings, Erin B / Isenburg, Jennifer L / Rutkowski, Rachel E / Kirby, Russell S / Nembhard, Wendy N / Sandidge, Theresa / Villavicencio, Stephan / Nguyen, Hoang H / McMahon, Daria M / Nestoridi, Eirini / Pabst, Laura J

Birth defects research

2024 Volume 116, Issue 1, Page(s) e2301

Abstract: Background: We provide updated crude and adjusted prevalence estimates of major birth defects in the United States for the period 2016-2020.: Methods: Data were collected from 13 US population-based surveillance programs that used active or a ... ...

Abstract	Background: We provide updated crude and adjusted prevalence estimates of major birth defects in the United States for the period 2016-2020. Methods: Data were collected from 13 US population-based surveillance programs that used active or a combination of active and passive case ascertainment methods to collect all birth outcomes. These data were used to calculate pooled prevalence estimates and national prevalence estimates adjusted for maternal race/ethnicity for all conditions, and maternal age for trisomies and gastroschisis. Prevalence was compared to previously published national estimates from 1999 to 2014. Results: Adjusted national prevalence estimates per 10,000 live births ranged from 0.63 for common truncus to 18.65 for clubfoot. Temporal changes were observed for several birth defects, including increases in the prevalence of atrioventricular septal defect, tetralogy of Fallot, omphalocele, trisomy 18, and trisomy 21 (Down syndrome) and decreases in the prevalence of anencephaly, common truncus, transposition of the great arteries, and cleft lip with and without cleft palate. Conclusion: This study provides updated national estimates of selected major birth defects in the United States. These data can be used for continued temporal monitoring of birth defects prevalence. Increases and decreases in prevalence since 1999 observed in this study warrant further investigation.
MeSH term(s)	Humans ; Down Syndrome ; Gastroschisis/epidemiology ; Heart Defects, Congenital/epidemiology ; Maternal Age ; Transposition of Great Vessels ; United States/epidemiology ; Female
Language	English
Publishing date	2024-01-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	2104792-3
ISSN	2472-1727
ISSN (online)	2472-1727
DOI	10.1002/bdr2.2301
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Article ; Online: The effect of intraoperative intrathecal opioid administration on the length of stay and postoperative pain control for patients undergoing lumbar interbody fusion.

Villavicencio, Alan / Taha, Hash Brown / Nelson, E Lee / Rajpal, Sharad / Beasley, Kara / Burneikiene, Sigita

Acta neurochirurgica

2022 Volume 164, Issue 11, Page(s) 3061–3069

Abstract: ... 20.8, p = 0.047; β = - 16.4, p = 0.030). Mean LOS was 15.4 h less in the ITO group (mean ± SD, 63.4 ...

Abstract	Purpose: In an effort to control postoperative pain more effectively in spinal fusion patients, intraoperative intrathecal morphine (ITM) administration is gaining popularity and acceptance with clinicians. This study seeks to determine the impact of intraoperative intrathecal opioid (ITO) administration following lumbar fusion surgery on postoperative pain and length of hospitalization as primary outcomes. Secondary outcomes will investigate postoperative opioid intake and side effects. Methods: The retrospective analysis of collected data was performed. The study compared patients undergoing one- or two-level transforaminal interbody fusions between 2019 and 2021 who intraoperatively received two different ITO doses (n = 89) vs. the reference group (n = 48) that did not receive ITO. The patients in the ITO group received either 0.2 mg (n = 44) of duramorph or 0.2 mg duramorph + 50 mcg fentanyl (n = 45). The effect of ITO was evaluated for the first four postoperative days (POD) on pain scores (visual analog scale), length of stay (LOS, hours) and opioid requirement (MED, morphine equivalent dose). Results: In the ITO group, a significant reduction of postoperative pain scores (t(99) = 4.3, p < 0.001) and opioid intake (t(70) = 2.49, p = 0.015) was noted on POD1. Cohen's d effect sizes were 0.76 and 0.50, meaning that postoperative pain and MED intake were reduced by about ¾ to ½ standard deviations (SD) in the ITO group. Further, multivariate regression models revealed that ITO administration predicted lower postoperative pain scores for the two PODs (β = - 0.83, p < 0.001; β = - 0.63, p = 0.022) and MED intake for the first two PODs (β = - 20.8, p = 0.047; β = - 16.4, p = 0.030). Mean LOS was 15.4 h less in the ITO group (mean ± SD, 63.4 ± 37.1 vs. 78.8 ± 39.6, p = 0.10). Conclusions: In conclusion, our study provides results in a large sample of patients undergoing transforaminal lumbar fusions. The results demonstrated that ITO administration is effective in reducing POD1 pain scores and POD1-2 opioid requirement while not increasing the risk of any opioid-related side effects.
MeSH term(s)	Humans ; Analgesics, Opioid/therapeutic use ; Analgesics, Opioid/adverse effects ; Morphine/adverse effects ; Retrospective Studies ; Length of Stay ; Injections, Spinal ; Pain, Postoperative/drug therapy ; Pain, Postoperative/prevention & control ; Spinal Fusion/methods
Chemical Substances	Analgesics, Opioid ; Morphine (76I7G6D29C)
Language	English
Publishing date	2022-09-17
Publishing country	Austria
Document type	Journal Article
ZDB-ID	80010-7
ISSN	0942-0940 ; 0001-6268
ISSN (online)	0942-0940
ISSN	0001-6268
DOI	10.1007/s00701-022-05359-8
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Article ; Online: Optimizing electronic blood ordering and supporting administration workflows to improve blood utilization in the pediatric hospital setting.

Stoffel, Michelle / Leu, Michael G / Barry, Dwight / Hrachovec, Jennifer / Saifee, Nabiha H / Migita, Darren S / Deam, Nate / Villavicencio, Carlos E / O'Hare, M Pauline / Pagliarulo, Amy / Delaney, Meghan

Transfusion

2023 Volume 63, Issue 12, Page(s) 2328–2340

Abstract: Background: Red blood cell wastage occurs when blood is discarded rather than transfused, and ineffective ordering results in unnecessary crossmatch procedures. We describe how a multimodal approach to redesigning electronic ordering tools improved ... ...

Abstract	Background: Red blood cell wastage occurs when blood is discarded rather than transfused, and ineffective ordering results in unnecessary crossmatch procedures. We describe how a multimodal approach to redesigning electronic ordering tools improved blood utilization in a pediatric inpatient setting and how using innovative application of time series data analysis provides insights into intervention effectiveness, which can guide future process improvement cycles. Methods: A multidisciplinary team used best practices and Toyota Production System methodology to redesign electronic blood ordering and improve administration processes. We analyzed crossmatch to transfusion ratio and red blood cell wastage time series data extracted from our laboratory information system and electronic health record. We used changepoint analysis to identify statistically discernible breaks in each time series, compatible with known interventions. We performed causal impact analysis on red blood cell wastage time series data to estimate blood wastage avoided due to the interventions. Results: Changepoint analysis estimated an 11% decrease in crossmatch to transfusion ratio and a 77% decrease in red blood cell monthly wastage rate during the intervention period. Causal impact analysis estimated a 61% reduction in expected wastage compared to the scenario if the interventions had not occurred. Discussion: Our results show that electronic health record design is an important factor in reducing waste and preventing unnecessary crossmatching, and that time series analysis can be a useful tool for evaluating the long-term impact of each stage of intervention in a longitudinal process redesign effort for the purpose of effectively targeting future improvement efforts.
MeSH term(s)	Humans ; Child ; Hospitals, Pediatric ; Workflow ; Blood Transfusion/methods ; Blood Grouping and Crossmatching ; Erythrocytes
Language	English
Publishing date	2023-11-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	208417-x
ISSN	1537-2995 ; 0041-1132
ISSN (online)	1537-2995
ISSN	0041-1132
DOI	10.1111/trf.17587
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Article ; Online: Effect of exposure to low doses of ozone on interleukin 17A expression during progressive neurodegeneration in the rat hippocampus.

Solleiro-Villavicencio, H / Hernández-Orozco, E / Rivas-Arancibia, S

Neurologia

2018

Title translation	Efecto de la exposición a bajas dosis de ozono en la expresión de la interleucina 17A durante el proceso de neurodegeneración progresiva en el hipocampo de ratas.
Abstract	Introduction: Chronic exposure to low doses of ozone causes oxidative stress and loss of regulation of the inflammatory response, leading to progressive neurodegeneration. Objective: We studied the effect of chronic exposure to low doses of ozone on IL-17A concentration and expression in neurons, microglia, astrocytes, and T cells in the rat hippocampus. Methods: We used 72 Wistar rats, divided into 6 groups (n=12): a control group (no ozone exposure) and 5 groups exposed to ozone (0.25ppm, 4h daily) for 7, 15, 30, 60, and 90 days. We processed 6 rats from each group to quantify IL-17A by ELISA; the remaining 6 were processed for immunohistochemistry (against IL-17A and GFAP, Iba1, NeuN, and CD3). Results: The ELISA study data showed a significant increase in IL-17A concentrations in the 7-, 15-, 30-, and 60-day exposure groups, with regard to the control group (P<.05). Furthermore, they indicate that hippocampal neurons were the cells showing greatest immunoreactivity against IL-17A between 60 and 90 days of exposure to ozone; we also observed an increase in activated astrocytes in the 30- and 60-day exposure groups. Conclusion: Exposure to ozone in rats induces an increase in IL-17A expression, mainly in hippocampal neurons, accompanied by hippocampal astrocyte activation during chronic neurodegeneration, similar to that observed in Alzheimer disease in humans.
Language	Spanish
Publishing date	2018-11-14
Publishing country	Spain
Document type	Journal Article
ZDB-ID	2654369-2
ISSN	2173-5808 ; 2173-5808
ISSN (online)	2173-5808
ISSN	2173-5808
DOI	10.1016/j.nrl.2018.08.001
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Article ; Online: Global, regional, and national causes of death in children and adolescents younger than 20 years: an open data portal with estimates for 2000-21.

Villavicencio, Francisco / Perin, Jamie / Eilerts-Spinelli, Hallie / Yeung, Diana / Prieto-Merino, David / Hug, Lucia / Sharrow, David / You, Danzhen / Strong, Kathleen L / Black, Robert E / Liu, Li

The Lancet. Global health

2023 Volume 12, Issue 1, Page(s) e16–e17

MeSH term(s)	Child ; Humans ; Adolescent ; Infant ; Cause of Death ; Causality ; Child Mortality ; Global Health
Language	English
Publishing date	2023-10-25
Publishing country	England
Document type	Journal Article
ZDB-ID	2723488-5
ISSN	2214-109X ; 2214-109X
ISSN (online)	2214-109X
ISSN	2214-109X
DOI	10.1016/S2214-109X(23)00496-5
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Article ; Online: Forensic parameters and population structure based on 21 autosomal STRs with the investigator 24plex QS in mestizos from the Mexico City population.

Salinas-Pineda, Lizette / Berumen, Jaime / Lopez Armenta, Mauro / Leon Campos, Carolina / Rangel-Villalobos, Hector / Juarez-Torres, Eligia / Huerta-Pacheco, N Sofia / Aguilar-Velazquez, Jose Alonso / Villavicencio-Queijeiro, Alexa / Guardado-Estrada, Mariano

Legal medicine (Tokyo, Japan)

2023 Volume 66, Page(s) 102344

Abstract: Allele frequencies and forensic parameters for 21 STR autosomal markers (CSF1PO, D10S1248, D12S391, D13S317,D16S539, D18S51, D19S433, D1S1656,D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, FGA, SE33, TH01, TPOX and vWA) were ... ...

Abstract	Allele frequencies and forensic parameters for 21 STR autosomal markers (CSF1PO, D10S1248, D12S391, D13S317,D16S539, D18S51, D19S433, D1S1656,D21S11, D22S1045, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, FGA, SE33, TH01, TPOX and vWA) were reported in 289 unrelated individuals from Mexico City, Mexico. In addition, an interpopulation analysis was performed including other world populations. In brief, the established population database of 21 autosomal STR markers in the present work is adequate for human identification purposes.
MeSH term(s)	Humans ; Genetics, Population ; Mexico ; Microsatellite Repeats/genetics ; DNA Fingerprinting ; Gene Frequency
Language	English
Publishing date	2023-11-08
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	2019555-2
ISSN	1873-4162 ; 1344-6223
ISSN (online)	1873-4162
ISSN	1344-6223
DOI	10.1016/j.legalmed.2023.102344
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Article: Vertical transmission and pathological findings in the mother, the placenta and the offspring, during first and last thirds of gestation in a mouse model of congenital toxoplasmosis

Vargas-Villavicencio, J.A. / Cedillo-Peláez, C. / Aguilar-Orozco, M.I. / Rico-Torres, C.P. / Farfan-Morales, J.E. / Correa, D.

Parasitology international. 2022 Dec., v. 91

2022

Abstract: ... euthanized them 72 h later. The tissues were analyzed by histopathology, immunohistochemistry and parasite ...

Abstract	We performed a study of congenital toxoplasmosis of the first and third gestation periods in mice, and determined its effects on the embryos/fetuses, the placentae and the maternal organs. We infected pregnant BALB/c mice by i.v. injection of 2.5‐–10.0 × 10⁶ tachyzoites of the ME49 T. gondii strain and euthanized them 72 h later. The tissues were analyzed by histopathology, immunohistochemistry and parasite-specific qPCR. Infections with the lowest dose induced remarkably different changes in the two thirds: a) all doses diminished the number of products/litter, the lowest dose only by 14%; but most embryos still visible were degenerated in the case of the first period, while the fetuses of the last third were perfectly preserved; b) the transmission rate in the first third was relatively high, but with a very low parasite burden; c) with the lowest dose, strong vascular changes (congestion, thrombosis and hemorrhage) predominated in the placentas of the first period, while they were absent in the last third; d) necrosis caused by T. gondii to maternal organs was much stronger during the last gestation period than in the first. Our results suggest that the vascular alterations at the placenta of the first third of pregnancy prevent embryo from large parasite burden, but provoke its death by starvation. In the last gestation period, there was poor control of parasite dissemination to the placenta and the fetus, but there was greater capacity of the product to defend itself from T. gondii.
Keywords	death ; fetus ; gestation period ; hemorrhage ; histopathology ; immunohistochemistry ; mice ; necrosis ; parasites ; parasitology ; placenta ; pregnancy ; progeny ; starvation ; tachyzoites ; thrombosis ; toxoplasmosis
Language	English
Dates of publication	2022-12
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1363151-2
ISSN	1383-5769
ISSN	1383-5769
DOI	10.1016/j.parint.2022.102640
Database	NAL-Catalogue (AGRICOLA)

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